NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

12204R-2

Symbol

CG12204

Full Name

CG12204

CG No

CG12204

Old CG No

CG12204

Synonyms

CG12204

Accession No (Link to NCBI)

NM_133158.2

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Molnar C, Casado M, López-Varea A, Cruz C, de Celis JF.<br> Genetic annotation of gain-of-function screens using RNA interference and in situ hybridization of candidate genes in the Drosophila wing.<br> Genetics (2012) 192(2) 741-52 [ PubMed ID = 22798488 ] [ RRC reference ]

Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.<br> Zyxin links fat signaling to the hippo pathway.<br> PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGTAGCCACAGACCACAGGACA

Primer Seq. 3'

aatctagaggtaccTTGCGTCCTTTGCTGGGATTG

Predicted Fragment Size

IR fragment full Seq

0001 GTAGCCACAG ACCACAGGAC AAAAACGGCG CAACTAAATC GGAGGTGTTA GAGCCATTGC 
0061 CATTGCCGGC GTCATGCAGC AACAATCCCG ACTTTAGCAT CCAGCTCGAT GACTCGACTA 
0121 TTCAGGGCAT TGTGGGCACC GAAAGAGCAC GACGCCTCCT TCCTGCTGCC TCCTTTGATG 
0181 ATNCTGCAGG CAGGACTCGA CGCGAGTCAG CGGCTGCTCA TCTACGAGCA CGTTCTGCAG 
0241 CACACCAAGA AGCACCCGCA GTTCCAGGAC AACACCGCCT TCGCCGAGGT GGTGGGCGAA 
0301 ACCCGGCGTC AGCAAAAGAA TCATCGGCGC CACCGCAAGA GCAAGCCCAC GCTCAACGAG 
0361 GAGCTGCACC AGCTAGTCAG CCTGCAGATG CAGGCGCTGG AGAAGCAGTG GGAACAGGAG 
0421 CACGGCGGGC GGCATCATAA GCAAGTCGAC CGACAGGAGC ACCGCGGACG GTATGTTGAG 
0481 CAATCCCAGC AAAGGACGCA A

in silico PCR Fragment

0001 GTAGCCACAG ACCACAGGAC AAAAACGGCG CAACTAAATC GGAGGTGTTA GAGCCATTGC 
0061 CATTGCCGGC GTCATGCAGC AACAATCCCG ACTTTAGCAT CCAGCTCGAT GACTCGACTA 
0121 TTCAGGGCAT TGTGGGCACC GAAAGAGCAC GACGCCTCCT TCCTGCTGCC TCCTTTGATG 
0181 AT-CTGCAGG CAGGACTCGA CGCGAGTCAG CGGCTGCTCA TCTACGAGCA CGTTCTAAAG 
0241 CACACCAAGA AGCACCCGCA GTTCCAGGAC AACACCGCCT TCGCCGAGGT GGTGGGCGAA 
0301 ACCCGGCGTC AGCAAAAGAA TCATCGGCGC CACCGCAAGA GCAAGCCCAC GCTCAACGAG 
0361 GAGCTGCACC AGCTAGTCAG CCTGCAGATG CAGGCGCTGG AGAAGCAGTG GGAACAGGAG 
0421 CACGGCGGGC GGCATCATAA GCAAGTCGAC CGACAGGAGC ACCGCGGACG GTATGTTGAG 
0481 CAATCCCAGC AAAGGACGCA A

Assemble Data

<PRE>

12204R-2.IR_full       1   GTAGCCACAGACCACAGGACAAAAACGGCGCAACTAAATCGGAGGTGTTAGAGCCATTGC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12204R-2.in silico     1   GTAGCCACAGACCACAGGACAAAAACGGCGCAACTAAATCGGAGGTGTTAGAGCCATTGC 60


12204R-2.IR_full       61  CATTGCCGGCGTCATGCAGCAACAATCCCGACTTTAGCATCCAGCTCGATGACTCGACTA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12204R-2.in silico     61  CATTGCCGGCGTCATGCAGCAACAATCCCGACTTTAGCATCCAGCTCGATGACTCGACTA 120


12204R-2.IR_full       121 TTCAGGGCATTGTGGGCACCGAAAGAGCACGACGCCTCCTTCCTGCTGCCTCCTTTGATG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12204R-2.in silico     121 TTCAGGGCATTGTGGGCACCGAAAGAGCACGACGCCTCCTTCCTGCTGCCTCCTTTGATG 180


12204R-2.IR_full       181 AT<span class="snp"><tt>N</tt></span>CTGCAGGCAGGACTCGACGCGAGTCAGCGGCTGCTCATCTACGAGCACGTTCT<span class="snp"><tt>G</tt></span><span class="snp"><tt>C</tt></span>AG 240
                           ||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>||
12204R-2.in silico     181 AT<span class="snp"><tt>-</tt></span>CTGCAGGCAGGACTCGACGCGAGTCAGCGGCTGCTCATCTACGAGCACGTTCT<span class="snp"><tt>A</tt></span><span class="snp"><tt>A</tt></span>AG 240


12204R-2.IR_full       241 CACACCAAGAAGCACCCGCAGTTCCAGGACAACACCGCCTTCGCCGAGGTGGTGGGCGAA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12204R-2.in silico     241 CACACCAAGAAGCACCCGCAGTTCCAGGACAACACCGCCTTCGCCGAGGTGGTGGGCGAA 300


12204R-2.IR_full       301 ACCCGGCGTCAGCAAAAGAATCATCGGCGCCACCGCAAGAGCAAGCCCACGCTCAACGAG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12204R-2.in silico     301 ACCCGGCGTCAGCAAAAGAATCATCGGCGCCACCGCAAGAGCAAGCCCACGCTCAACGAG 360


12204R-2.IR_full       361 GAGCTGCACCAGCTAGTCAGCCTGCAGATGCAGGCGCTGGAGAAGCAGTGGGAACAGGAG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12204R-2.in silico     361 GAGCTGCACCAGCTAGTCAGCCTGCAGATGCAGGCGCTGGAGAAGCAGTGGGAACAGGAG 420


12204R-2.IR_full       421 CACGGCGGGCGGCATCATAAGCAAGTCGACCGACAGGAGCACCGCGGACGGTATGTTGAG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12204R-2.in silico     421 CACGGCGGGCGGCATCATAAGCAAGTCGACCGACAGGAGCACCGCGGACGGTATGTTGAG 480


12204R-2.IR_full       481 CAATCCCAGCAAAGGACGCAA 501
                           |||||||||||||||||||||
12204R-2.in silico     481 CAATCCCAGCAAAGGACGCAA 501

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	2	11	NM_133158.2	CG12204-RA (CG12204), mRNA
0.41	2	2	6	7	NM_169450.1	CG14741-RA (CG14741), mRNA
0.2	1	2	6	2	NM_058048.3	CG11324-RA, transcript variant A (homer), mRNA
0.2	1	2	6	2	NM_001042876.1	CG11324-RC, transcript variant C (homer), mRNA
0.2	1	2	6	2	NM_164706.1	CG11324-RB, transcript variant B (homer), mRNA
0.2	1	2	2	6	NM_136043.2	CG10413-RA (CG10413), mRNA
0	0	4	3	2	NM_057242.3	CG7765-RA (Khc), mRNA
0	0	3	3	3	NM_135024.1	CG11926-RA (CG11926), mRNA
0	0	3	3	2	NM_136365.1	CG11212-RA (Ptr), mRNA
0	0	3	2	4	NM_057752.3	CG9378-RA, transcript variant A (Rlc1), mRNA
0	0	3	2	4	NM_176433.1	CG9378-RB, transcript variant B (Rlc1), mRNA
0	0	2	5	2	NM_143750.2	CG6133-RA (CG6133), mRNA
0	0	2	4	3	NM_140876.1	CG8798-RA, transcript variant A (CG8798), mRNA
0	0	2	4	3	NM_168806.1	CG8798-RB, transcript variant B (CG8798), mRNA
0	0	2	3	4	NM_143021.2	CG5796-RA (Ppox), mRNA
0	0	2	3	2	NM_170162.1	CG31126-RA (CG31126), mRNA
0	0	2	2	8	NM_080047.2	CG8491-RA (kto), mRNA
0	0	2	2	3	NM_132838.2	CG8231-RA (l(1)G0022), mRNA
0	0	2	2	2	NM_078598.1	CG14413-RA (mRpS25), mRNA
0	0	1	6	3	NM_137192.1	CG12958-RA (CG12958), mRNA
0	0	1	6	2	NM_078559.2	CG18085-RA (sev), mRNA
0	0	1	5	5	NM_139675.2	CG7479-RA (Aats-leu), mRNA
0	0	1	4	10	NM_136397.1	CG15236-RA, transcript variant A (CG15236), mRNA
0	0	1	4	10	NM_165493.1	CG15236-RB, transcript variant B (CG15236), mRNA
0	0	1	4	7	NM_132519.1	CG9369-RA (m), mRNA
0	0	1	4	6	NM_133079.2	CG6481-RA (CG6481), mRNA
0	0	1	4	3	NM_078866.2	CG6549-RA, transcript variant A (fws), mRNA
0	0	1	4	3	NM_165221.1	CG6549-RC, transcript variant C (fws), mRNA
0	0	1	3	4	NM_057737.2	CG15804-RA, transcript variant A (Dhc62B), mRNA
0	0	1	3	4	NM_206236.1	CG15804-RB, transcript variant B (Dhc62B), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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