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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 12189R-3

Symbol Rev1 Full Name Rev1

CG No CG12189 Old CG No CG12189

Synonyms DmREV1, CG12189, AAF47401, drev1, DmRev1, dREV1, REV1, Rev1

Accession No (Link to NCBI) NM_138203.2

Inserted Chr. X

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
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Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATTTGTGAACGGCCGGACTGA

Primer Seq. 3'

aatctagaggtaccTGCGAGCGGTGGTGGAGTTAT

Predicted Fragment Size

IR fragment full Seq

0001 ATTTGTGAAC GGCCGGACTG ACCCTTCGGC GGATGAACTG AAGCGTATTA TGATGGTGCA 
0061 CGGCGGGACC TTTCACCACT ACGAAAGGAG CCACACGACG TACATAATAG CCTCCGTTCT 
0121 GCCCGATGTG AAGGTCAGGA ACATGAATCT CAGCAAGTTC ATCAGCGCCA AGTGGGTGGT 
0181 GGATTGTCTG GAGAAAAAGA AGATAGTAGA CTACAAACCC TATCTGCTGT ACACGAACCA 
0241 GAAGACATCG CAGCCCATGC TCATCTTTGG GAAACCCAAA GACAACGGCG CAAATGAGAG 
0301 CAAATCGGAT GTGGAGCCGC CGAAAGATAA AGCGGAAGTG GAAGTAGACT CTACAAAAGA 
0361 TGAAACGCAA ATGGAGTTGG GTGGCATTCT CAAGAATTTG CAGCAGGCTG TGGCTACTTC 
0421 GCCGGAAAAG GAGGCCAGTG CATCAGAGAG CAAGATCACA AACTTATCCA CCACCTCGAA 
0481 TAACTCCACC ACCGCTCGCA

in silico PCR Fragment

0001 ATTTGTGAAC GGCCGGACTG ACCCTTCGGC GGATGAACTG AAGCGTATTA TGATGGTGCA 
0061 CGGCGGGACC TTTCACCACT ACGAAAGGAG CCACACGACG TACATAATAG CCTCCGTTCT 
0121 GCCCGATGTG AAGGTCAGGA ACATGAATCT CAGCAAGTTC ATCAGCGCCA AGTGGGTGGT 
0181 GGATTGTCTG GAGAAAAAGA AGATAGTAGA CTACAAACCC TATCTGCTGT ACACGAACCA 
0241 GAAGACATCG CAGCCCATGC TCATCTTTGG GAAACCCAAA GACAACGGCG CAAATGAGAG 
0301 CAAATCGGAT GTGGAGCCGC CGAAAGATAA AGCGGAAGTG GAAGTAGACT CTACAAAAGA 
0361 TGAAACGCAA ATGGAGTTGG GTGGCATTCT CAAGAATTTG CAGCAGGCTG TGGCCACTTC 
0421 GCCGGAAAAG GAGGCCAGTG CATCAGAGAG CAAGATCACA AACTTATCCA CCACCTCGAA 
0481 TAACTCCACC ACCGCTCGCA

Assemble Data


12189R-3.IR_full       1   ATTTGTGAACGGCCGGACTGACCCTTCGGCGGATGAACTGAAGCGTATTATGATGGTGCA 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12189R-3.in silico     1   ATTTGTGAACGGCCGGACTGACCCTTCGGCGGATGAACTGAAGCGTATTATGATGGTGCA 60


12189R-3.IR_full       61  CGGCGGGACCTTTCACCACTACGAAAGGAGCCACACGACGTACATAATAGCCTCCGTTCT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12189R-3.in silico     61  CGGCGGGACCTTTCACCACTACGAAAGGAGCCACACGACGTACATAATAGCCTCCGTTCT 120


12189R-3.IR_full       121 GCCCGATGTGAAGGTCAGGAACATGAATCTCAGCAAGTTCATCAGCGCCAAGTGGGTGGT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12189R-3.in silico     121 GCCCGATGTGAAGGTCAGGAACATGAATCTCAGCAAGTTCATCAGCGCCAAGTGGGTGGT 180


12189R-3.IR_full       181 GGATTGTCTGGAGAAAAAGAAGATAGTAGACTACAAACCCTATCTGCTGTACACGAACCA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12189R-3.in silico     181 GGATTGTCTGGAGAAAAAGAAGATAGTAGACTACAAACCCTATCTGCTGTACACGAACCA 240


12189R-3.IR_full       241 GAAGACATCGCAGCCCATGCTCATCTTTGGGAAACCCAAAGACAACGGCGCAAATGAGAG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12189R-3.in silico     241 GAAGACATCGCAGCCCATGCTCATCTTTGGGAAACCCAAAGACAACGGCGCAAATGAGAG 300


12189R-3.IR_full       301 CAAATCGGATGTGGAGCCGCCGAAAGATAAAGCGGAAGTGGAAGTAGACTCTACAAAAGA 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12189R-3.in silico     301 CAAATCGGATGTGGAGCCGCCGAAAGATAAAGCGGAAGTGGAAGTAGACTCTACAAAAGA 360


12189R-3.IR_full       361 TGAAACGCAAATGGAGTTGGGTGGCATTCTCAAGAATTTGCAGCAGGCTGTGGCTACTTC 420
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||
12189R-3.in silico     361 TGAAACGCAAATGGAGTTGGGTGGCATTCTCAAGAATTTGCAGCAGGCTGTGGCCACTTC 420


12189R-3.IR_full       421 GCCGGAAAAGGAGGCCAGTGCATCAGAGAGCAAGATCACAAACTTATCCACCACCTCGAA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12189R-3.in silico     421 GCCGGAAAAGGAGGCCAGTGCATCAGAGAGCAAGATCACAAACTTATCCACCACCTCGAA 480


12189R-3.IR_full       481 TAACTCCACCACCGCTCGCA 500
                           ||||||||||||||||||||
12189R-3.in silico     481 TAACTCCACCACCGCTCGCA 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	2	6	NM_138203.2	CG12189-RA (Rev1), mRNA
0	0	4	3	10	NM_142538.1	CG5237-RA (CG5237), mRNA
0	0	3	3	2	NM_141399.1	CG9727-RA (CG9727), mRNA
0	0	3	2	2	NM_167239.2	CG32677-RA (CG32677), mRNA
0	0	2	4	3	NM_139641.2	CG7447-RA, transcript variant A (CG7447), mRNA
0	0	2	4	3	NM_176283.1	CG7447-RB, transcript variant B (CG7447), mRNA
0	0	2	3	9	NM_142051.2	CG9345-RA (Adgf-C), mRNA
0	0	2	3	4	NM_136954.2	CG8594-RA (CG8594), mRNA
0	0	2	2	2	NM_136457.2	CG1358-RA, transcript variant A (CG1358), mRNA
0	0	2	2	2	NM_165545.1	CG1358-RB, transcript variant B (CG1358), mRNA
0	0	2	2	2	NM_165546.1	CG1358-RC, transcript variant C (CG1358), mRNA
0	0	2	2	2	NM_137051.1	CG13337-RA (CG13337), mRNA
0	0	1	4	2	NM_164463.1	CG7082-RD, transcript variant D (CG7082), mRNA
0	0	1	4	2	NM_164462.1	CG7082-RB, transcript variant B (CG7082), mRNA
0	0	1	4	2	NM_134813.2	CG7082-RC, transcript variant C (CG7082), mRNA
0	0	1	4	2	NM_164461.1	CG7082-RA, transcript variant A (CG7082), mRNA
0	0	1	3	4	NM_137314.2	CG5522-RC, transcript variant C (CG5522), mRNA
0	0	1	3	4	NM_166193.1	CG5522-RA, transcript variant A (CG5522), mRNA
0	0	1	3	4	NM_166194.1	CG5522-RB, transcript variant B (CG5522), mRNA
0	0	1	3	4	NM_166197.1	CG5522-RF, transcript variant F (CG5522), mRNA
0	0	1	3	4	NM_166195.1	CG5522-RE, transcript variant E (CG5522), mRNA
0	0	1	3	4	NM_166196.1	CG5522-RD, transcript variant D (CG5522), mRNA
0	0	1	2	6	NM_169698.1	CG5000-RA (msps), mRNA
0	0	1	2	5	NM_001038883.1	CG33988-RA (CG33988), mRNA
0	0	1	2	2	NM_142086.2	CG9649-RA (CG9649), mRNA
0	0	0	6	4	NM_137455.4	CG10915-RA (CG10915), mRNA
0	0	0	6	3	NM_144376.2	CG1656-RA (lectin-46Ca), mRNA
0	0	0	5	6	NM_137120.1	CG17386-RA (CG17386), mRNA
0	0	0	5	3	NM_138200.2	CG12030-RA (CG12030), mRNA
0	0	0	4	8	NM_165355.1	CG9256-RA, transcript variant A (Nhe2), mRNA

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