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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

12172R-1

Symbol

Spn43Aa

Full Name

Serine protease inhibitor 43Aa

CG No

CG12172

Old CG No

CG12172

Synonyms

Ser1, SER1, anon-WO0118057.1, CG12172, Spn43Aa

Accession No (Link to NCBI)

NM_080066.2

Inserted Chr.

lll

Insertional Mutation

lethal

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCATTCTTCTTGGGGTGTGGAT

Primer Seq. 3'

aatctagaggtaccTGTCCGGCTCCAGGCTTTCCA

Predicted Fragment Size

IR fragment full Seq

0001 CATTCTTCTT GGGGTGTGGA TATCCGCTCC TGAAGGTCTG GGTAACACGA TCAAGGATCG 
0061 CAATCTCTTC GCCACCGAAC TCTTCCAAAC CCTCGCCACA GATCGCCAGG ATGAGAACGT 
0121 GATCATCTCG CCGGTTTCCA TCCAGCTGGC CCTCGGGTTG GCTTACTACG GAGCTGAGGG 
0181 CAGGACGGCC GCGGAACTGC AGAAGACCTT GCACGCCTCC GCCAAGGAGA GCAAAGATGG 
0241 TCTGGCCGAG AGCTACCACA ACCTGCTGCA CTCTTACATC AAGTCCAAGA CCGTGTTGGA 
0301 GATCGCCAAC AAGGTGTACA CCCGGCAGAA TCTCACGGTA TCTAGCCACT TCCGAGAGGT 
0361 GGCCCAGAAG TACTTCGACT CCGAGGTAGA ACCACTGGAC TTCAGTCGCG AAACGGAGGC 
0421 CGTGGAGCAG ATCAACCGCT GGGTGAAGCA GCAGACGGAG AACAAGATCG AACGGGTGGT 
0481 GGAAAGCCTG GAGCCGGACA

in silico PCR Fragment

0001 CATTCTTCTT GGGGTGTGGA TATCCGCTCC TGAAGGTCTG GGTAACACGA TCAAGGATCG 
0061 CAATCTCTTC GCCACCGAAC TCTTCCAAAC CCTCGCCACA GATCGCCAGG ATGAGAACGT 
0121 GATCATCTCG CCGGTTTCCA TCCAGCTGGC CCTCGGGTTG GCTTACTACG GAGCTGAGGG 
0181 CAGGACGGCC GCGGAACTGC AGAAGACCTT GCACGCCTCC GCCAAGGAGA GCAAAGATGG 
0241 TCTGGCCGAG AGCTACCACA ACCTGCTGCA CTCTTACATC AAGTCCAAGA CCGTGTTGGA 
0301 GATCGCCAAC AAGGTGTACA CCCGGCAGAA TCTCACGGTA TCTAGCCACT TCCGAGAGGT 
0361 GGCCCAGAAG TACTTCGACT CCGAGGTAGA ACCACTGGAC TTCAGTCGCG AAACGGAGGC 
0421 CGTGGAGCAG ATCAACCGCT GGGTGAAGCA GCAGACGGAG AACAAGATCG AACGGGTGGT 
0481 GGAAAGCCTG GAGCCGGACA

Assemble Data


12172R-1.IR_full       1   CATTCTTCTTGGGGTGTGGATATCCGCTCCTGAAGGTCTGGGTAACACGATCAAGGATCG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12172R-1.in silico     1   CATTCTTCTTGGGGTGTGGATATCCGCTCCTGAAGGTCTGGGTAACACGATCAAGGATCG 60


12172R-1.IR_full       61  CAATCTCTTCGCCACCGAACTCTTCCAAACCCTCGCCACAGATCGCCAGGATGAGAACGT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12172R-1.in silico     61  CAATCTCTTCGCCACCGAACTCTTCCAAACCCTCGCCACAGATCGCCAGGATGAGAACGT 120


12172R-1.IR_full       121 GATCATCTCGCCGGTTTCCATCCAGCTGGCCCTCGGGTTGGCTTACTACGGAGCTGAGGG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12172R-1.in silico     121 GATCATCTCGCCGGTTTCCATCCAGCTGGCCCTCGGGTTGGCTTACTACGGAGCTGAGGG 180


12172R-1.IR_full       181 CAGGACGGCCGCGGAACTGCAGAAGACCTTGCACGCCTCCGCCAAGGAGAGCAAAGATGG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12172R-1.in silico     181 CAGGACGGCCGCGGAACTGCAGAAGACCTTGCACGCCTCCGCCAAGGAGAGCAAAGATGG 240


12172R-1.IR_full       241 TCTGGCCGAGAGCTACCACAACCTGCTGCACTCTTACATCAAGTCCAAGACCGTGTTGGA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12172R-1.in silico     241 TCTGGCCGAGAGCTACCACAACCTGCTGCACTCTTACATCAAGTCCAAGACCGTGTTGGA 300


12172R-1.IR_full       301 GATCGCCAACAAGGTGTACACCCGGCAGAATCTCACGGTATCTAGCCACTTCCGAGAGGT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12172R-1.in silico     301 GATCGCCAACAAGGTGTACACCCGGCAGAATCTCACGGTATCTAGCCACTTCCGAGAGGT 360


12172R-1.IR_full       361 GGCCCAGAAGTACTTCGACTCCGAGGTAGAACCACTGGACTTCAGTCGCGAAACGGAGGC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12172R-1.in silico     361 GGCCCAGAAGTACTTCGACTCCGAGGTAGAACCACTGGACTTCAGTCGCGAAACGGAGGC 420


12172R-1.IR_full       421 CGTGGAGCAGATCAACCGCTGGGTGAAGCAGCAGACGGAGAACAAGATCGAACGGGTGGT 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12172R-1.in silico     421 CGTGGAGCAGATCAACCGCTGGGTGAAGCAGCAGACGGAGAACAAGATCGAACGGGTGGT 480


12172R-1.IR_full       481 GGAAAGCCTGGAGCCGGACA 500
                           ||||||||||||||||||||
12172R-1.in silico     481 GGAAAGCCTGGAGCCGGACA 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	8	NM_080066.2	CG12172-RA (Spn43Aa), mRNA
0	0	4	2	3	NM_078723.2	CG3022-RA, transcript variant A (GABA-B-R3), mRNA
0	0	3	2	3	NM_057466.2	CG8896-RA (18w), mRNA
0	0	3	2	2	NM_167722.1	CG10998-RB, transcript variant B (r-cup), mRNA
0	0	3	2	2	NM_134575.2	CG10998-RA, transcript variant A (r-cup), mRNA
0	0	2	6	8	NM_143767.2	CG11331-RA (Spn27A), mRNA
0	0	2	2	4	NM_134690.2	CG2807-RA (CG2807), mRNA
0	0	2	2	3	NM_141865.1	CG6959-RA, transcript variant A (CG6959), mRNA
0	0	2	2	3	NM_206475.1	CG6959-RB, transcript variant B (CG6959), mRNA
0	0	1	7	9	NM_139839.3	CG32381-RA (unc-13-4A), mRNA
0	0	1	5	4	NM_144026.1	CG11458-RA (CG11458), mRNA
0	0	1	4	3	NM_078510.2	CG4039-RA (Mcm6), mRNA
0	0	1	3	8	NM_136455.1	CG2093-RA (CG2093), mRNA
0	0	1	3	4	NM_080301.2	CG3848-RC, transcript variant C (trr), mRNA
0	0	1	3	4	NM_166911.3	CG3848-RD, transcript variant D (trr), mRNA
0	0	1	2	9	NM_142636.1	CG5191-RB, transcript variant B (CG5191), mRNA
0	0	1	2	9	NM_169904.1	CG5191-RA, transcript variant A (CG5191), mRNA
0	0	1	2	9	NM_169902.1	CG5191-RC, transcript variant C (CG5191), mRNA
0	0	1	2	9	NM_169905.1	CG5191-RD, transcript variant D (CG5191), mRNA
0	0	1	2	9	NM_169903.1	CG5191-RE, transcript variant E (CG5191), mRNA
0	0	1	2	3	NM_140058.2	CG3529-RB (CG3529), mRNA
0	0	1	2	3	NM_167243.1	CG32675-RC, transcript variant C (Tango5), mRNA
0	0	1	2	3	NM_167242.1	CG32675-RA, transcript variant A (Tango5), mRNA
0	0	1	2	3	NM_167244.1	CG32675-RB, transcript variant B (Tango5), mRNA
0	0	0	37	66	NM_079919.2	CG5700-RB (prc), mRNA
0	0	0	6	3	NM_176553.1	CG6129-RC, transcript variant C (CG6129), mRNA
0	0	0	6	3	NM_142959.2	CG6129-RB, transcript variant B (CG6129), mRNA
0	0	0	6	2	NM_167335.1	CG4013-RC, transcript variant C (Smr), mRNA
0	0	0	6	2	NM_167334.1	CG4013-RB, transcript variant B (Smr), mRNA
0	0	0	6	2	NM_080536.2	CG4013-RA, transcript variant A (Smr), mRNA

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