|
Notice:
|
|
Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
|
|
| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
12052R-3 |
| Symbol |
lola |
Full Name |
longitudinals lacking |
| CG No |
CG12052 |
Old CG No |
CG12052 |
| Synonyms |
CG12052, eyeful, CG18381, CG18379, CG18378, CG30012, BTB-IV, LD03274, l(2)00642, l(2)s3697, bs06a08.y1, NEST:bs06a08, BcDNA:RH31485, sw59, CG30014, CG30013, CG18380, CG18376, BtbIV, BEST:LD03274, BcDNA:LD17006, lola |
| Accession No (Link to NCBI) |
NM_170623.4 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCGTGTTCGACACGTTGCTGGA |
| Primer Seq. 3' |
aatctagaggtaccTGCTGCGACGTCGGACTTTTC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CGTGTTCGAC ACGTTGCTGG AGAACGAGAC TCTAGTCGAT TGCACGCTAG CCGCCGAGGG
0061 CAAATTTCTC AAGGCCCACA AGGTGGTGCT GTCAGCATGC AGTCCCTACT TTGCTACCTT
0121 ACTACAAGAA CAGTACGACA AACATCCCAT CTTTATACTC AAGGATGTCA AGTACCAAGA
0181 GCTGCGCGCC ATGATGGACT ACATGTACCG CGGCGAGGTC AATATCTCGC AGGATCAGCT
0241 GGCCGCTCTG CNTCAAGGCC GCCGAATCGC TTCAGATCAA GGGCCTTTCG GACAATCGCA
0301 CTGGCGGCGG AGTAGCTCCC AAGCCANGAG TCCTCCGGCC ATCATCGCGG CGGTAAGCTG
0361 AGCGGTGCCT ACACACTGGA GCAAACTAAG CGGGCTCGAC TGGCCACCGG CGGAGCGATG
0421 GATACGTCTG GCGATGTGTC CGGTTCAGCG CGAGGGCTCC TCGAGTCCGT CgNGTCGTCG
0481 CCGAAAAGTC CGACGTCGCA GCA |
| in silico PCR Fragment |
0001 CGTGTTCGAC ACGTTGCTGG AGAACGAGAC TCTAGTCGAT TGCACGCTAG CCGCCGAGGG
0061 CAAATTTCTC AAGGCCCACA AGGTGGTGCT GTCAGCATGC AGTCCCTACT TTGCTACCTT
0121 ACTACAAGAA CAGTACGACA AACATCCCAT CTTTATACTC AAGGATGTCA AGTACCAAGA
0181 GCTGCGCGCC ATGATGGACT ACATGTACCG CGGCGAGGTC AATATCTCGC AGGATCAGCT
0241 GGCCGCTCTG C-TCAAGGCC GCCGAATCGC TTCAGATCAA GGGCCTTTCG GACAATCGCA
0301 CTGGCGGCGG AGTAGCTCCC AAGCCA-GAG TCCTCCGGCC ATCATCGCGG CGGTAAGCTG
0361 AGCGGTGCCT ACACACTGGA GCAAACTAAG CGGGCTCGAC TGGCCACCGG CGGAGCGATG
0421 GATACGTCTG GCGATGTGTC CGGTTC-GCG CGAGGGCTCC TCGAGTCCGT CGCGTCGTCG
0481 CCGAAAAGTC CGACGTCGCA GCA |
| Assemble Data |
<PRE>
12052R-3.IR_full 1 CGTGTTCGACACGTTGCTGGAGAACGAGACTCTAGTCGATTGCACGCTAGCCGCCGAGGG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12052R-3.in silico 1 CGTGTTCGACACGTTGCTGGAGAACGAGACTCTAGTCGATTGCACGCTAGCCGCCGAGGG 60
12052R-3.IR_full 61 CAAATTTCTCAAGGCCCACAAGGTGGTGCTGTCAGCATGCAGTCCCTACTTTGCTACCTT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12052R-3.in silico 61 CAAATTTCTCAAGGCCCACAAGGTGGTGCTGTCAGCATGCAGTCCCTACTTTGCTACCTT 120
12052R-3.IR_full 121 ACTACAAGAACAGTACGACAAACATCCCATCTTTATACTCAAGGATGTCAAGTACCAAGA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12052R-3.in silico 121 ACTACAAGAACAGTACGACAAACATCCCATCTTTATACTCAAGGATGTCAAGTACCAAGA 180
12052R-3.IR_full 181 GCTGCGCGCCATGATGGACTACATGTACCGCGGCGAGGTCAATATCTCGCAGGATCAGCT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12052R-3.in silico 181 GCTGCGCGCCATGATGGACTACATGTACCGCGGCGAGGTCAATATCTCGCAGGATCAGCT 240
12052R-3.IR_full 241 GGCCGCTCTGC<span class="snp"><tt>N</tt></span>TCAAGGCCGCCGAATCGCTTCAGATCAAGGGCCTTTCGGACAATCGCA 300
|||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||||||||||||
12052R-3.in silico 241 GGCCGCTCTGC<span class="snp"><tt>-</tt></span>TCAAGGCCGCCGAATCGCTTCAGATCAAGGGCCTTTCGGACAATCGCA 300
12052R-3.IR_full 301 CTGGCGGCGGAGTAGCTCCCAAGCCA<span class="snp"><tt>N</tt></span>GAGTCCTCCGGCCATCATCGCGGCGGTAAGCTG 360
||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||
12052R-3.in silico 301 CTGGCGGCGGAGTAGCTCCCAAGCCA<span class="snp"><tt>-</tt></span>GAGTCCTCCGGCCATCATCGCGGCGGTAAGCTG 360
12052R-3.IR_full 361 AGCGGTGCCTACACACTGGAGCAAACTAAGCGGGCTCGACTGGCCACCGGCGGAGCGATG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12052R-3.in silico 361 AGCGGTGCCTACACACTGGAGCAAACTAAGCGGGCTCGACTGGCCACCGGCGGAGCGATG 420
12052R-3.IR_full 421 GATACGTCTGGCGATGTGTCCGGTTC<span class="snp"><tt>A</tt></span>GCGCGAGGGCTCCTCGAGTCCGTCG<span class="snp"><tt>N</tt></span>GTCGTCG 480
||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||
12052R-3.in silico 421 GATACGTCTGGCGATGTGTCCGGTTC<span class="snp"><tt>-</tt></span>GCGCGAGGGCTCCTCGAGTCCGTCG<span class="snp"><tt>C</tt></span>GTCGTCG 480
12052R-3.IR_full 481 CCGAAAAGTCCGACGTCGCAGCA 503
|||||||||||||||||||||||
12052R-3.in silico 481 CCGAAAAGTCCGACGTCGCAGCA 503
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
3 |
NM_176139.2 |
CG12052-RQ, transcript variant Q (lola), mRNA |
| 100 |
482 |
0 |
0 |
3 |
NM_170621.3 |
CG12052-RE, transcript variant E (lola), mRNA |
| 100 |
482 |
0 |
0 |
3 |
NM_170620.2 |
CG12052-RD, transcript variant D (lola), mRNA |
| 100 |
482 |
0 |
0 |
1 |
NM_176129.3 |
CG12052-RO, transcript variant O (lola), mRNA |
| 100 |
482 |
0 |
0 |
1 |
NM_170618.3 |
CG12052-RC, transcript variant C (lola), mRNA |
| 100 |
482 |
0 |
0 |
1 |
NM_170619.2 |
CG12052-RB, transcript variant B (lola), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_176140.2 |
CG12052-RM, transcript variant M (lola), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_176138.3 |
CG12052-RL, transcript variant L (lola), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_170623.4 |
CG12052-RA, transcript variant A (lola), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_001032229.1 |
CG12052-RZ, transcript variant Z (lola), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_176137.3 |
CG12052-RS, transcript variant S (lola), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_206085.2 |
CG12052-RV, transcript variant V (lola), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_176136.3 |
CG12052-RK, transcript variant K (lola), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_170622.4 |
CG12052-RF, transcript variant F (lola), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_176132.2 |
CG12052-RR, transcript variant R (lola), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_080027.4 |
CG12052-RG, transcript variant G (lola), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_206083.2 |
CG12052-RX, transcript variant X (lola), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_206082.2 |
CG12052-RY, transcript variant Y (lola), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_176128.3 |
CG12052-RN, transcript variant N (lola), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_206084.2 |
CG12052-RW, transcript variant W (lola), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_176130.3 |
CG12052-RJ, transcript variant J (lola), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_176131.3 |
CG12052-RP, transcript variant P (lola), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_170617.3 |
CG12052-RH, transcript variant H (lola), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_176133.3 |
CG12052-RI, transcript variant I (lola), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_176134.2 |
CG12052-RT, transcript variant T (lola), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_176135.3 |
CG12052-RU, transcript variant U (lola), mRNA |
| 0.2 |
1 |
4 |
4 |
6 |
NM_132049.3 |
CG12236-RB, transcript variant B (CG12236), mRNA |
| 0.2 |
1 |
4 |
4 |
6 |
NM_167054.1 |
CG12236-RA, transcript variant A (CG12236), mRNA |
| 0 |
0 |
3 |
8 |
16 |
NM_163886.1 |
CG32491-RP, transcript variant P (mod(mdg4)), mRNA |
| 0 |
0 |
3 |
8 |
16 |
NM_163888.1 |
CG32491-RK, transcript variant K (mod(mdg4)), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
|
JNK signal
|
Caspase-3 signal
|
|
|
|
Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
|
|
|
