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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 12034R-2 
 Symbol CG12034  Full Name CG12034 
 CG No CG12034  Old CG No CG12034 
 Synonyms nSMase, CG12034 
 Accession No (Link to NCBI) NM_139533.2 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees semi-lethal 
 Map Viewer
 Reference
[Please submit your publication] 		 
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgATGTTGCTGCTGGAGCTGAAC 
 Primer Seq. 3' 
aatctagaggtaccGAGTTCCCCCTGGTGGCTTCA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 ATGTTGCTGC TGGAGCTGAA CATTCTGACC CTGAATATAT GGGGCATTCC GTATGTGTCG 
0061 AGTGATCGCA GACCCCGCAT CGATGCCATC TGCAAGGAGC TGGCTAGCGG GAAGTATGAC 
0121 ATCGTGTCCC TGCAGGAGGT GTGGGCGCAA GAGGACAGCG AGCTACTCCA AAAGGGCACG 
0181 GAGGCGGTCC TGCCCCATAG CCACTACTTT CACAGCGGCG TGATAGGCGC CGGTCTTGCT 
0241 GGTACTGTCC AAGTACCCCA TCCTGGGCAC TCTGTTTCAT GCGTGGTCCG TAAATGGCTA 
0301 CTTCCACCGT ATTCAGCATG CCGATTGGTT CGGCGGCAAA GGTGTGGGCC TGTGCCGCAT 
0361 ACTCGTCGGC GGGCAGATGG TGCACCTATA TAATGCCCAC CTGCACGCGG AGTACGACAA 
0421 TGCCAACGAC GAGTACAAGA CACATCGCGT TATTCAGGCC TTCGATACTG CTCAATTCAT 
0481 TGAAGCCACC AGGGGGAACT C 
 in silico PCR Fragment 
0001 ATGTTGCTGC TGGAGCTGAA CATTCTGACC CTGAATATAT GGGGCATTCC GTATGTGTCG 
0061 AGTGATCGCA GACCCCGCAT CGATGCCATC TGCAAGGAGC TGGCTAGCGG GAAGTATGAC 
0121 ATCGTGTCCC TGCAGGAGGT GTGGGCGCAA GAGGACAGCG AGCTACTCCA AAAGGGCACG 
0181 GAGGCGGTCC TGCCCCATAG CCACTACTTT CACAGCGGCG TGATGGGCGC CGGTC-TGCT 
0241 GGTACTGTCC AAGTACCCCA TCCTGGGCAC TCTGTTTCAT GCGTGGTCCG TAAATGGCTA 
0301 CTTCCACCGT ATTCAGCATG CCGATTGGTT CGGCGGCAAA GGTGTGGGCC TGTGCCGCAT 
0361 ACTCGTCGGC GGGCAGATGG TGCACCTATA TAATGCCCAC CTGCACGCGG AGTACGACAA 
0421 TGCCAACGAC GAGTACAAGA CACATCGCGT TATTCAGGCC TTCGATACTG CTCAATTCAT 
0481 TGAAGCCACC AGGGGGAACT C 
 Assemble Data 
<PRE>

12034R-2.IR_full       1   ATGTTGCTGCTGGAGCTGAACATTCTGACCCTGAATATATGGGGCATTCCGTATGTGTCG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12034R-2.in silico     1   ATGTTGCTGCTGGAGCTGAACATTCTGACCCTGAATATATGGGGCATTCCGTATGTGTCG 60


12034R-2.IR_full       61  AGTGATCGCAGACCCCGCATCGATGCCATCTGCAAGGAGCTGGCTAGCGGGAAGTATGAC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12034R-2.in silico     61  AGTGATCGCAGACCCCGCATCGATGCCATCTGCAAGGAGCTGGCTAGCGGGAAGTATGAC 120


12034R-2.IR_full       121 ATCGTGTCCCTGCAGGAGGTGTGGGCGCAAGAGGACAGCGAGCTACTCCAAAAGGGCACG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12034R-2.in silico     121 ATCGTGTCCCTGCAGGAGGTGTGGGCGCAAGAGGACAGCGAGCTACTCCAAAAGGGCACG 180


12034R-2.IR_full       181 GAGGCGGTCCTGCCCCATAGCCACTACTTTCACAGCGGCGTGAT<span class="snp"><tt>A</tt></span>GGCGCCGGTC<span class="snp"><tt>T</tt></span>TGCT 240
                           ||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||
12034R-2.in silico     181 GAGGCGGTCCTGCCCCATAGCCACTACTTTCACAGCGGCGTGAT<span class="snp"><tt>G</tt></span>GGCGCCGGTC<span class="snp"><tt>-</tt></span>TGCT 240


12034R-2.IR_full       241 GGTACTGTCCAAGTACCCCATCCTGGGCACTCTGTTTCATGCGTGGTCCGTAAATGGCTA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12034R-2.in silico     241 GGTACTGTCCAAGTACCCCATCCTGGGCACTCTGTTTCATGCGTGGTCCGTAAATGGCTA 300


12034R-2.IR_full       301 CTTCCACCGTATTCAGCATGCCGATTGGTTCGGCGGCAAAGGTGTGGGCCTGTGCCGCAT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12034R-2.in silico     301 CTTCCACCGTATTCAGCATGCCGATTGGTTCGGCGGCAAAGGTGTGGGCCTGTGCCGCAT 360


12034R-2.IR_full       361 ACTCGTCGGCGGGCAGATGGTGCACCTATATAATGCCCACCTGCACGCGGAGTACGACAA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12034R-2.in silico     361 ACTCGTCGGCGGGCAGATGGTGCACCTATATAATGCCCACCTGCACGCGGAGTACGACAA 420


12034R-2.IR_full       421 TGCCAACGACGAGTACAAGACACATCGCGTTATTCAGGCCTTCGATACTGCTCAATTCAT 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12034R-2.in silico     421 TGCCAACGACGAGTACAAGACACATCGCGTTATTCAGGCCTTCGATACTGCTCAATTCAT 480


12034R-2.IR_full       481 TGAAGCCACCAGGGGGAACTC 501
                           |||||||||||||||||||||
12034R-2.in silico     481 TGAAGCCACCAGGGGGAACTC 501

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_139533.2  CG12034-RA (CG12034), mRNA 
0   0  3  2  3  NM_176162.1  CG33138-RA (CG33138), mRNA 
0   0  2  3  5  NM_079224.3  CG10037-RA (vvl), mRNA 
0   0  1  5  4  NM_143102.1  CG10423-RA (RpS27), mRNA 
0   0  1  3  4  NM_078902.2  CG8276-RB, transcript variant B (bin3), mRNA 
0   0  1  3  4  NM_165468.1  CG8276-RA, transcript variant A (bin3), mRNA 
0   0  1  3  1  NM_057345.2  CG10325-RA, transcript variant A (abd-A), mRNA 
0   0  1  3  1  NM_169733.2  CG10325-RB, transcript variant B (abd-A), mRNA 
0   0  1  2  6  NM_142215.2  CG5205-RA (CG5205), mRNA 
0   0  1  2  3  NM_080340.2  CG2302-RA (nAcRalpha-7E), mRNA 
0   0  1  1  1  NM_143694.1  CG11152-RA (CG11152), mRNA 
0   0  1  1  1  NM_001014630.1  CG33547-RA (Rim), mRNA 
0   0  0  8  5  NM_132522.2  CG32662-RA (CG32662), mRNA 
0   0  0  6  9  NM_167646.1  CG32827-RA (CG32827), mRNA 
0   0  0  6  5  NM_079662.2  CG5400-RA (Eh), mRNA 
0   0  0  5  12  NM_132435.1  CG1582-RA (CG1582), mRNA 
0   0  0  5  3  NM_080138.2  CG1618-RA (comt), mRNA 
0   0  0  4  9  NM_165218.1  CG6667-RB, transcript variant B (dl), mRNA 
0   0  0  4  9  NM_165217.1  CG6667-RA, transcript variant A (dl), mRNA 
0   0  0  4  4  NM_140189.1  CG7616-RA (CG7616), mRNA 
0   0  0  4  3  NM_058023.4  CG4900-RA (Irp-1A), mRNA 
0   0  0  4  2  NM_078931.2  CG1877-RD, transcript variant D (lin19), mRNA 
0   0  0  4  2  NM_132724.2  CG1810-RA (mRNA-capping-enzyme), mRNA 
0   0  0  4  2  NM_165571.1  CG1877-RC, transcript variant C (lin19), mRNA 
0   0  0  4  2  NM_166244.1  CG30109-RA (CG30109), mRNA 
0   0  0  4  2  NM_165569.1  CG1877-RA, transcript variant A (lin19), mRNA 
0   0  0  4  2  NM_079579.2  CG6342-RA (Irp-1B), mRNA 
0   0  0  4  2  NM_165570.1  CG1877-RB, transcript variant B (lin19), mRNA 
0   0  0  3  9  NM_168571.2  CG32133-RA (CG32133), mRNA 
0   0  0  3  9  NM_143320.2  CG18437-RA (CG18437), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway dme00600 : Sphingolipid metabolism
dme01100 : Metabolic pathways

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