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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
12034R-1 |
| Symbol |
CG12034 |
Full Name |
CG12034 |
| CG No |
CG12034 |
Old CG No |
CG12034 |
| Synonyms |
nSMase, CG12034 |
| Accession No (Link to NCBI) |
NM_139533.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
2 lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
semi-lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Swarup S, Pradhan-Sundd T, Verheyen EM.<br> Genome-wide identification of phospho-regulators of Wnt signaling in Drosophila.<br> Development (2015) 142(8) 1502-15
[
PubMed ID = 25852200
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.<br> An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.<br> PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATGTTGCTGCTGGAGCTGAAC |
| Primer Seq. 3' |
aatctagaggtaccGAGTTCCCCCTGGTGGCTTCA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ATGTTGCTGC TGGAGCTGAA CATTCTGACC CTGAATATAT GGGGCATTCC GTATGTGTCG
0061 AGTGATCGCA GACCCCGCAT CGATGCCATC TGCAAGGAGC TGGCTAGCGG GAAGTATGAC
0121 ATCGTGTCCC TGCAGGAGGT GTGGGCGCAA GAGGACAGCG AGCTACTCCA AAAGGGCACG
0181 GAGGCGGTCC TGCCCCATAG CCACTACTTT CACAGCGGCG TGATAGGCGC CGGTCTTGCT
0241 GGTACTGTCC AAGTACCCCA TCCTGGGCAC TCTGTTTCAT GCGTGGTCCG TAAATGGCTA
0301 CTTCCACCGT ATTCAGCATG CCGATTGGTT CGGCGGCAAA GGTGTGGGCC TGTGCCGCAT
0361 ACTCGTCGGC GGGCAGATGG TGCACCTATA TAATGCCCAC CTGCACGCGG AGTACGACAA
0421 TGCCAACGAC GAGTACAAGA CACATCGCGT TATTCAGGCC TTCGATACTG CTCAATTCAT
0481 TGAAGCCACC AGGGGGAACT C |
| in silico PCR Fragment |
0001 ATGTTGCTGC TGGAGCTGAA CATTCTGACC CTGAATATAT GGGGCATTCC GTATGTGTCG
0061 AGTGATCGCA GACCCCGCAT CGATGCCATC TGCAAGGAGC TGGCTAGCGG GAAGTATGAC
0121 ATCGTGTCCC TGCAGGAGGT GTGGGCGCAA GAGGACAGCG AGCTACTCCA AAAGGGCACG
0181 GAGGCGGTCC TGCCCCATAG CCACTACTTT CACAGCGGCG TGATGGGCGC CGGTC-TGCT
0241 GGTACTGTCC AAGTACCCCA TCCTGGGCAC TCTGTTTCAT GCGTGGTCCG TAAATGGCTA
0301 CTTCCACCGT ATTCAGCATG CCGATTGGTT CGGCGGCAAA GGTGTGGGCC TGTGCCGCAT
0361 ACTCGTCGGC GGGCAGATGG TGCACCTATA TAATGCCCAC CTGCACGCGG AGTACGACAA
0421 TGCCAACGAC GAGTACAAGA CACATCGCGT TATTCAGGCC TTCGATACTG CTCAATTCAT
0481 TGAAGCCACC AGGGGGAACT C |
| Assemble Data |
<PRE>
12034R-1.IR_full 1 ATGTTGCTGCTGGAGCTGAACATTCTGACCCTGAATATATGGGGCATTCCGTATGTGTCG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12034R-1.in silico 1 ATGTTGCTGCTGGAGCTGAACATTCTGACCCTGAATATATGGGGCATTCCGTATGTGTCG 60
12034R-1.IR_full 61 AGTGATCGCAGACCCCGCATCGATGCCATCTGCAAGGAGCTGGCTAGCGGGAAGTATGAC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12034R-1.in silico 61 AGTGATCGCAGACCCCGCATCGATGCCATCTGCAAGGAGCTGGCTAGCGGGAAGTATGAC 120
12034R-1.IR_full 121 ATCGTGTCCCTGCAGGAGGTGTGGGCGCAAGAGGACAGCGAGCTACTCCAAAAGGGCACG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12034R-1.in silico 121 ATCGTGTCCCTGCAGGAGGTGTGGGCGCAAGAGGACAGCGAGCTACTCCAAAAGGGCACG 180
12034R-1.IR_full 181 GAGGCGGTCCTGCCCCATAGCCACTACTTTCACAGCGGCGTGAT<span class="snp"><tt>A</tt></span>GGCGCCGGTC<span class="snp"><tt>T</tt></span>TGCT 240
||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||<span class="snp"><tt> </tt></span>||||
12034R-1.in silico 181 GAGGCGGTCCTGCCCCATAGCCACTACTTTCACAGCGGCGTGAT<span class="snp"><tt>G</tt></span>GGCGCCGGTC<span class="snp"><tt>-</tt></span>TGCT 240
12034R-1.IR_full 241 GGTACTGTCCAAGTACCCCATCCTGGGCACTCTGTTTCATGCGTGGTCCGTAAATGGCTA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12034R-1.in silico 241 GGTACTGTCCAAGTACCCCATCCTGGGCACTCTGTTTCATGCGTGGTCCGTAAATGGCTA 300
12034R-1.IR_full 301 CTTCCACCGTATTCAGCATGCCGATTGGTTCGGCGGCAAAGGTGTGGGCCTGTGCCGCAT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12034R-1.in silico 301 CTTCCACCGTATTCAGCATGCCGATTGGTTCGGCGGCAAAGGTGTGGGCCTGTGCCGCAT 360
12034R-1.IR_full 361 ACTCGTCGGCGGGCAGATGGTGCACCTATATAATGCCCACCTGCACGCGGAGTACGACAA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12034R-1.in silico 361 ACTCGTCGGCGGGCAGATGGTGCACCTATATAATGCCCACCTGCACGCGGAGTACGACAA 420
12034R-1.IR_full 421 TGCCAACGACGAGTACAAGACACATCGCGTTATTCAGGCCTTCGATACTGCTCAATTCAT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12034R-1.in silico 421 TGCCAACGACGAGTACAAGACACATCGCGTTATTCAGGCCTTCGATACTGCTCAATTCAT 480
12034R-1.IR_full 481 TGAAGCCACCAGGGGGAACTC 501
|||||||||||||||||||||
12034R-1.in silico 481 TGAAGCCACCAGGGGGAACTC 501
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_139533.2 |
CG12034-RA (CG12034), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_176162.1 |
CG33138-RA (CG33138), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_079224.3 |
CG10037-RA (vvl), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_143102.1 |
CG10423-RA (RpS27), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_078902.2 |
CG8276-RB, transcript variant B (bin3), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_165468.1 |
CG8276-RA, transcript variant A (bin3), mRNA |
| 0 |
0 |
1 |
3 |
1 |
NM_057345.2 |
CG10325-RA, transcript variant A (abd-A), mRNA |
| 0 |
0 |
1 |
3 |
1 |
NM_169733.2 |
CG10325-RB, transcript variant B (abd-A), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_142215.2 |
CG5205-RA (CG5205), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_080340.2 |
CG2302-RA (nAcRalpha-7E), mRNA |
| 0 |
0 |
1 |
1 |
1 |
NM_143694.1 |
CG11152-RA (CG11152), mRNA |
| 0 |
0 |
1 |
1 |
1 |
NM_001014630.1 |
CG33547-RA (Rim), mRNA |
| 0 |
0 |
0 |
8 |
5 |
NM_132522.2 |
CG32662-RA (CG32662), mRNA |
| 0 |
0 |
0 |
6 |
9 |
NM_167646.1 |
CG32827-RA (CG32827), mRNA |
| 0 |
0 |
0 |
6 |
5 |
NM_079662.2 |
CG5400-RA (Eh), mRNA |
| 0 |
0 |
0 |
5 |
12 |
NM_132435.1 |
CG1582-RA (CG1582), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_080138.2 |
CG1618-RA (comt), mRNA |
| 0 |
0 |
0 |
4 |
9 |
NM_165218.1 |
CG6667-RB, transcript variant B (dl), mRNA |
| 0 |
0 |
0 |
4 |
9 |
NM_165217.1 |
CG6667-RA, transcript variant A (dl), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_140189.1 |
CG7616-RA (CG7616), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_058023.4 |
CG4900-RA (Irp-1A), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_078931.2 |
CG1877-RD, transcript variant D (lin19), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_132724.2 |
CG1810-RA (mRNA-capping-enzyme), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_165571.1 |
CG1877-RC, transcript variant C (lin19), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_166244.1 |
CG30109-RA (CG30109), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_165569.1 |
CG1877-RA, transcript variant A (lin19), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_079579.2 |
CG6342-RA (Irp-1B), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_165570.1 |
CG1877-RB, transcript variant B (lin19), mRNA |
| 0 |
0 |
0 |
3 |
9 |
NM_168571.2 |
CG32133-RA (CG32133), mRNA |
| 0 |
0 |
0 |
3 |
9 |
NM_143320.2 |
CG18437-RA (CG18437), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
|
JNK signal
|
Caspase-3 signal
| N |
 |
| |
|
| DB |
 |
| + |
|
| DC |
 |
| + |
|
| DM |
 |
| ++ |
|
| VM |
 |
| ++ |
|
| VB |
 |
| |
|
|
| Dcompartment |
|
Penetrance |
| + |
|
|
|
|
|
Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
dme00600
:
Sphingolipid metabolism
dme01100
:
Metabolic pathways
|
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