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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 12010R-3 
 Symbol CG12010  Full Name CG12010 
 CG No CG12010  Old CG No CG12010 
 Synonyms CG12010 
 Accession No (Link to NCBI) NM_139555.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees larval lethal 
 Map Viewer
 Reference
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 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgTCAGCGGTTGGTGCTTCTGGA 
 Primer Seq. 3' 
aatctagaggtaccTGCAGCTATGACGACGCAGGA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 TCAGCGGTTG GTGCTTCTGG AGCCACTTAA ATTAGTTCCC TGCGTGAAGA GGATTTCATG 
0061 CGTGATTACA GTCACCCAGG AGCTCCTTCA GCGACCTCCG GTTTCCCTGG AGGATCTGAA 
0121 GGACGATGTG CGAATCTTCC TGCGCCACCT GAAGCTTACC AAAGGATGCT CCGTGCAGCA 
0181 CAGACGCCTT GTGCAGCTGG GCATTGCCAG TGTGGAGATA TTCGATGCTC CAGGAGTTGC 
0241 TGATGACGAG TGTTTTGAAA TAGCCCACGA TGCTGAGCTA CTCCTGGCGG ACATACGCCT 
0301 GGGAATGCCC TTTTCTTTAG ATTTTATCTT CAAGTATTTG CCACACAGCT TCGAGCGACC 
0361 CATGGAGCAG TTGGAGAAGC TCCTGGAAAC CTCTAAAATC AGCTTTTCGC ATAGATTTCC 
0421 TACTACAGCA TTGGTTGTGG GTCCGGTGGG CTGTGGAAAA AGTGGTCTAC TCAGTGAGTT 
0481 CCTGCGTCGT CATAGCTGCA  
 in silico PCR Fragment 
0001 TCAGCGGTTG GTGCTTCTGG AGCCACTTAA ATTAGTTCCC TGCGTGAAGA GGATTTCATG 
0061 CGTGATTACA GTCACCCAGG AGCTCCTTCA GCGACCTCCG GTTTCCCTGG AGGATCTGAA 
0121 GGACGATGTG CGAATCTTCC TGCGCCACCT GAAGCTTACC AAAGGATGCT CCGTGCAGCA 
0181 CAGACGCCTT GTGCAGCTGG GCATTGCCAG TGTGGAGATA TTCGATGCTC CAGGAGTTGC 
0241 TGATGACGAG TGTTTTGAAA TAGCCCACGA TGCTGAGCTA CTCCTGGCGG ACATACGCCT 
0301 GGGAATGCCC TTTTCTTTAG ATTTTATCTT CAAGTATTTG CCACACAGCT TCGAGCGACC 
0361 CATGGAGCAG TTGGAGAAGC TCCTGGAAAC CTCTAAAATC AGCTTTTCGC ATAGATTTCC 
0421 TACTACAGCA TTGGTTGTGG GTCCGGTGGA CTGTGGAAAA AGTGGTCTAC TCAGTGAGTT 
0481 CCTGCGTCGT CATAGCTGCA  
 Assemble Data 
<PRE>

12010R-3.IR_full       1   TCAGCGGTTGGTGCTTCTGGAGCCACTTAAATTAGTTCCCTGCGTGAAGAGGATTTCATG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12010R-3.in silico     1   TCAGCGGTTGGTGCTTCTGGAGCCACTTAAATTAGTTCCCTGCGTGAAGAGGATTTCATG 60


12010R-3.IR_full       61  CGTGATTACAGTCACCCAGGAGCTCCTTCAGCGACCTCCGGTTTCCCTGGAGGATCTGAA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12010R-3.in silico     61  CGTGATTACAGTCACCCAGGAGCTCCTTCAGCGACCTCCGGTTTCCCTGGAGGATCTGAA 120


12010R-3.IR_full       121 GGACGATGTGCGAATCTTCCTGCGCCACCTGAAGCTTACCAAAGGATGCTCCGTGCAGCA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12010R-3.in silico     121 GGACGATGTGCGAATCTTCCTGCGCCACCTGAAGCTTACCAAAGGATGCTCCGTGCAGCA 180


12010R-3.IR_full       181 CAGACGCCTTGTGCAGCTGGGCATTGCCAGTGTGGAGATATTCGATGCTCCAGGAGTTGC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12010R-3.in silico     181 CAGACGCCTTGTGCAGCTGGGCATTGCCAGTGTGGAGATATTCGATGCTCCAGGAGTTGC 240


12010R-3.IR_full       241 TGATGACGAGTGTTTTGAAATAGCCCACGATGCTGAGCTACTCCTGGCGGACATACGCCT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12010R-3.in silico     241 TGATGACGAGTGTTTTGAAATAGCCCACGATGCTGAGCTACTCCTGGCGGACATACGCCT 300


12010R-3.IR_full       301 GGGAATGCCCTTTTCTTTAGATTTTATCTTCAAGTATTTGCCACACAGCTTCGAGCGACC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12010R-3.in silico     301 GGGAATGCCCTTTTCTTTAGATTTTATCTTCAAGTATTTGCCACACAGCTTCGAGCGACC 360


12010R-3.IR_full       361 CATGGAGCAGTTGGAGAAGCTCCTGGAAACCTCTAAAATCAGCTTTTCGCATAGATTTCC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12010R-3.in silico     361 CATGGAGCAGTTGGAGAAGCTCCTGGAAACCTCTAAAATCAGCTTTTCGCATAGATTTCC 420


12010R-3.IR_full       421 TACTACAGCATTGGTTGTGGGTCCGGTGG<span class="snp"><tt>G</tt></span>CTGTGGAAAAAGTGGTCTACTCAGTGAGTT 480
                           |||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||
12010R-3.in silico     421 TACTACAGCATTGGTTGTGGGTCCGGTGG<span class="snp"><tt>A</tt></span>CTGTGGAAAAAGTGGTCTACTCAGTGAGTT 480


12010R-3.IR_full       481 CCTGCGTCGTCATAGCTGCA 500
                           ||||||||||||||||||||
12010R-3.in silico     481 CCTGCGTCGTCATAGCTGCA 500

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  2  NM_139555.1  CG12010-RA, transcript variant A (CG12010), mRNA 
100   482  0  0  2  NM_168030.1  CG12010-RB, transcript variant B (CG12010), mRNA 
0   0  3  2  7  NM_001043010.1  CG9432-RD, transcript variant D (l(2)01289), mRNA 
0   0  3  2  6  NM_165485.1  CG9432-RB, transcript variant B (l(2)01289), mRNA 
0   0  3  2  6  NM_143769.3  CG9432-RA, transcript variant A (l(2)01289), mRNA 
0   0  3  2  2  NM_001043009.1  CG9432-RE, transcript variant E (l(2)01289), mRNA 
0   0  2  6  5  NM_165800.1  CG12342-RB, transcript variant B (CG12342), mRNA 
0   0  2  6  5  NM_136770.1  CG12342-RA, transcript variant A (CG12342), mRNA 
0   0  2  2  6  NM_078582.3  CG32659-RA (Ten-a), mRNA 
0   0  2  2  2  NM_139879.1  CG14837-RB, transcript variant B (CG14837), mRNA 
0   0  1  4  3  NM_142921.2  CG10225-RA (CG10225), mRNA 
0   0  1  3  3  NM_139838.2  CG14830-RA (CG14830), mRNA 
0   0  1  3  2  NM_176221.2  CG33198-RA (pen-2), mRNA 
0   0  1  3  2  NM_078666.2  CG8146-RA (Socs16D), mRNA 
0   0  1  2  5  NM_141667.2  CG8358-RA (CG8358), mRNA 
0   0  1  2  5  NM_080110.2  CG32356-RA, transcript variant A (ImpE1), mRNA 
0   0  1  2  5  NM_143032.1  CG6980-RA (CG6980), mRNA 
0   0  1  2  4  NM_169881.1  CG31213-RA (CG31213), mRNA 
0   0  1  2  2  NM_079050.2  CG6510-RA (RpL18A), mRNA 
0   0  0  6  17  NM_132831.1  CG8184-RB (CG8184), mRNA 
0   0  0  6  8  NM_130571.1  CG14796-RA (CG14796), mRNA 
0   0  0  6  5  NM_132704.1  CG11071-RA (CG11071), mRNA 
0   0  0  5  10  NM_176447.1  CG33208-RF, transcript variant F (MICAL), mRNA 
0   0  0  5  10  NM_176445.1  CG33208-RC, transcript variant C (MICAL), mRNA 
0   0  0  5  10  NM_176449.1  CG33208-RH, transcript variant H (MICAL), mRNA 
0   0  0  5  10  NM_176448.1  CG33208-RG, transcript variant G (MICAL), mRNA 
0   0  0  5  10  NM_176446.1  CG33208-RD, transcript variant D (MICAL), mRNA 
0   0  0  5  9  NM_137652.2  CG16742-RA, transcript variant A (CG16742), mRNA 
0   0  0  5  4  NM_170386.1  CG11876-RA, transcript variant A (CG11876), mRNA 
0   0  0  5  4  NM_170388.1  CG11876-RC, transcript variant C (CG11876), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway

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