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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
11901R-2 |
| Symbol |
Ef1gamma |
Full Name |
Ef1gamma |
| CG No |
CG11901 |
Old CG No |
CG11901 |
| Synonyms |
EF1gamma, Ef1Bggr, CG11901, Ef1gamma, eEF1gamma |
| Accession No (Link to NCBI) |
NM_143743.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K.<br> Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila.<br> PLoS Genet (2019) 15(4) e1008121
[
PubMed ID = 31034473
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
Fan Y, Schlierf M, Gaspar AC, Dreux C, Kpebe A, Chaney L, Mathieu A, Hitte C, Grémy O, Sarot E, Horn M, Zhao Y, Kinzy TG, Rabinow L.<br> Drosophila translational elongation factor-1gamma is modified in response to DOA kinase activity and is essential for cellular viability.<br> Genetics (2010) 184(1) 141-54
[
PubMed ID = 19841092
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGAGACCAACAAGTCGGCTGAG |
| Primer Seq. 3' |
aatctagaggtaccAGCTTGTAGTCCTTGACGACG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GAGACCAACA AGTCGGCTGA GTTCCTCAAG AAGTTCCCCG GTGGCAAGGT GCCCGCCTTT
0061 GAGACCGCCG AAGGACAGTA CTTGAGCGAG TCCAATGCCA TCGCCTACCT GCTGGCCAAC
0121 GAGCAGCTGC GCGGCGGAAA GTGCCCCTTC GTGCAGGCCC AGGTCCAGCA GTGGATCTCC
0181 TTCGCCGACA ACGAGATTGT GCCTGCCTCC TGTGCCTGGG TCTTCCCCCT GCTGGGCATC
0241 CTGCCGCAGC AGAAGAACAG CACTGCCAAG CAAGAGGCCG AGGCTGTGCT GCAGCAGCTC
0301 AACCAGAAGC TGCAGGACGC CACCTTNCCT GGCCGGCGAG AGGATCACAT TGGCCGACAT
0361 TGTGGTCTTC AGCAGTCTGC TCCACCTGTA CGAGTACGTC CTGGAGCCCA GTGTGCGNCA
0421 GTGCCTTCGG CAACGTGAAC CGCTGGTTCG TCACCATCCT CAACCAGAAG CAGGTCCAGG
0481 CCGTCGTCAA GGACTACAAG CT |
| in silico PCR Fragment |
0001 GAGACCAACA AGTCGGCTGA GTTCCTCAAG AAGTTCCCCG GTGGCAAGGT GCCCGCCTTT
0061 GAGACCGCCG AAGGACAGTA CTTGAGCGAG TCCAATGCCA TCGCCTACCT GCTGGCCAAC
0121 GAGCAGCTGC GCGGCGGAAA GTGCCCCTTC GTGCAGGCCC AGGTCCAGCA GTGGATCTCC
0181 TTCGCCGACA ACGAGATTGT GCCTGCCTCC TGTGCCTGGG TCTTCCCCCT GCTGGGCATT
0241 CTGCCGCAGC AGAAGAACAG CACTGCCAAG CAAGAGGCCG AGGCTGTGCT GCAGCAGCTC
0301 AACCAGAAGC TGCAGGACGC CACCTT-CCT GGCCGGCGAG AGGATCACAT TGGCCGACAT
0361 TGTGGTCTTC AGCAGTCTGC TCCACCTGTA CGAGTACGTC CTGGAGCCCA GTGTGCG-CA
0421 GTGCCTTCGG CAACGTGAAC CGCTGGTTCG TCACCATCCT CAACCAGAAG CAGGTCCAGG
0481 CCGTCGTCAA GGACTACAAG CT |
| Assemble Data |
<PRE>
11901R-2.IR_full 1 GAGACCAACAAGTCGGCTGAGTTCCTCAAGAAGTTCCCCGGTGGCAAGGTGCCCGCCTTT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11901R-2.in silico 1 GAGACCAACAAGTCGGCTGAGTTCCTCAAGAAGTTCCCCGGTGGCAAGGTGCCCGCCTTT 60
11901R-2.IR_full 61 GAGACCGCCGAAGGACAGTACTTGAGCGAGTCCAATGCCATCGCCTACCTGCTGGCCAAC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11901R-2.in silico 61 GAGACCGCCGAAGGACAGTACTTGAGCGAGTCCAATGCCATCGCCTACCTGCTGGCCAAC 120
11901R-2.IR_full 121 GAGCAGCTGCGCGGCGGAAAGTGCCCCTTCGTGCAGGCCCAGGTCCAGCAGTGGATCTCC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11901R-2.in silico 121 GAGCAGCTGCGCGGCGGAAAGTGCCCCTTCGTGCAGGCCCAGGTCCAGCAGTGGATCTCC 180
11901R-2.IR_full 181 TTCGCCGACAACGAGATTGTGCCTGCCTCCTGTGCCTGGGTCTTCCCCCTGCTGGGCAT<span class="snp"><tt>C</tt></span> 240
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>
11901R-2.in silico 181 TTCGCCGACAACGAGATTGTGCCTGCCTCCTGTGCCTGGGTCTTCCCCCTGCTGGGCAT<span class="snp"><tt>T</tt></span> 240
11901R-2.IR_full 241 CTGCCGCAGCAGAAGAACAGCACTGCCAAGCAAGAGGCCGAGGCTGTGCTGCAGCAGCTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11901R-2.in silico 241 CTGCCGCAGCAGAAGAACAGCACTGCCAAGCAAGAGGCCGAGGCTGTGCTGCAGCAGCTC 300
11901R-2.IR_full 301 AACCAGAAGCTGCAGGACGCCACCTT<span class="snp"><tt>N</tt></span>CCTGGCCGGCGAGAGGATCACATTGGCCGACAT 360
||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||
11901R-2.in silico 301 AACCAGAAGCTGCAGGACGCCACCTT<span class="snp"><tt>-</tt></span>CCTGGCCGGCGAGAGGATCACATTGGCCGACAT 360
11901R-2.IR_full 361 TGTGGTCTTCAGCAGTCTGCTCCACCTGTACGAGTACGTCCTGGAGCCCAGTGTGCG<span class="snp"><tt>N</tt></span>CA 420
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||
11901R-2.in silico 361 TGTGGTCTTCAGCAGTCTGCTCCACCTGTACGAGTACGTCCTGGAGCCCAGTGTGCG<span class="snp"><tt>-</tt></span>CA 420
11901R-2.IR_full 421 GTGCCTTCGGCAACGTGAACCGCTGGTTCGTCACCATCCTCAACCAGAAGCAGGTCCAGG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11901R-2.in silico 421 GTGCCTTCGGCAACGTGAACCGCTGGTTCGTCACCATCCTCAACCAGAAGCAGGTCCAGG 480
11901R-2.IR_full 481 CCGTCGTCAAGGACTACAAGCT 502
||||||||||||||||||||||
11901R-2.in silico 481 CCGTCGTCAAGGACTACAAGCT 502
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_170401.1 |
CG11901-RB, transcript variant B (Ef1gamma), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_143743.1 |
CG11901-RA, transcript variant A (Ef1gamma), mRNA |
| 0.2 |
1 |
3 |
3 |
9 |
NM_138088.2 |
CG11414-RA (CG11414), mRNA |
| 0 |
0 |
5 |
2 |
3 |
NM_080264.2 |
CG12767-RA (Dip3), mRNA |
| 0 |
0 |
4 |
2 |
37 |
NM_001032051.1 |
CG33715-RD, transcript variant D (Msp-300), mRNA |
| 0 |
0 |
4 |
2 |
23 |
NM_001032053.1 |
CG33715-RB, transcript variant B (Msp-300), mRNA |
| 0 |
0 |
3 |
7 |
4 |
NM_176203.1 |
CG4945-RC, transcript variant C (CG4945), mRNA |
| 0 |
0 |
3 |
7 |
4 |
NM_176202.1 |
CG4945-RB, transcript variant B (CG4945), mRNA |
| 0 |
0 |
3 |
4 |
6 |
NM_165696.1 |
CG1648-RB, transcript variant B (CG1648), mRNA |
| 0 |
0 |
3 |
4 |
6 |
NM_136674.2 |
CG1648-RA, transcript variant A (CG1648), mRNA |
| 0 |
0 |
3 |
3 |
5 |
NM_078639.2 |
CG13956-RB, transcript variant B (kat80), mRNA |
| 0 |
0 |
3 |
3 |
5 |
NM_167488.1 |
CG13956-RA, transcript variant A (kat80), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_206678.1 |
CG33175-RA, transcript variant A (spri), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_176723.2 |
CG33175-RG, transcript variant G (spri), mRNA |
| 0 |
0 |
2 |
5 |
6 |
NM_132322.2 |
CG15797-RA (ric8a), mRNA |
| 0 |
0 |
2 |
5 |
6 |
NM_078988.2 |
CG8529-RB, transcript variant B (Dyb), mRNA |
| 0 |
0 |
2 |
5 |
6 |
NM_165905.2 |
CG8529-RC, transcript variant C (Dyb), mRNA |
| 0 |
0 |
2 |
5 |
6 |
NM_165904.1 |
CG8529-RA, transcript variant A (Dyb), mRNA |
| 0 |
0 |
2 |
5 |
6 |
NM_001032236.1 |
CG8529-RE, transcript variant E (Dyb), mRNA |
| 0 |
0 |
2 |
5 |
6 |
NM_001032237.1 |
CG8529-RD, transcript variant D (Dyb), mRNA |
| 0 |
0 |
2 |
5 |
2 |
NM_166554.1 |
CG3612-RA (blw), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_132287.2 |
CG4040-RA (CG4040), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_057693.3 |
CG4799-RA (Pen), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_136840.2 |
CG7759-RB, transcript variant B (CG7759), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_165831.1 |
CG7759-RA, transcript variant A (CG7759), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_133077.1 |
CG6335-RB, transcript variant B (Aats-his), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_167624.1 |
CG6335-RA, transcript variant A (Aats-his), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_143463.3 |
CG7609-RB, transcript variant B (CG7609), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_170424.1 |
CG7609-RA, transcript variant A (CG7609), mRNA |
| 0 |
0 |
2 |
2 |
17 |
NM_057643.2 |
CG10844-RA, transcript variant A (Rya-r44F), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
|
JNK signal
|
Caspase-3 signal
| N |
 |
| ++ |
|
| DB |
 |
| + |
|
| DC |
 |
| |
|
| DM |
 |
| + |
|
| VM |
 |
| + |
|
| VB |
 |
| |
|
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Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
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