NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

11900R-1

Symbol

CG11900

Full Name

CG11900

CG No

CG11900

Old CG No

CG11900

Synonyms

CG11900

Accession No (Link to NCBI)

NM_143425.1

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGGAGTGCCTCCAATATGCAGC

Primer Seq. 3'

aatctagaggtaccCGCGTTGGCGGAAGATCTCGT

Predicted Fragment Size

IR fragment full Seq

0001 GGAGTGCCTC CAATATGCAG CTTTTAAGCA CCGGCAGCAA CGTCGCAAGG ATCCTCAGGA 
0061 AACCCCCTAT GTGAACCATG TGATCAATGT GAGCACTATT CTCTCCGTGG AGGCCTGCAT 
0121 CACAGATGAG GGAGTCCTAA TGGCTGCACT TCTGCACGAT GTCGTGGAGG ATACGGACGC 
0181 ATCTTTCGAG GATGTTGAGA AACTCTTTGG ACCGGATGTT TGTGGACTAG TGAGGGAGGT 
0241 GACGGACGAC AAGTCCCTGG AGAAACAGGA GAGAAAACGC CTGCAAATAG AAAATGCAGC 
0301 CAAAAGCAGC TGCAGAGCAA AGCTCATAAA GCTGGCTGAC AAATTGGATA ACCTAAGGGA 
0361 TCTGCAAGTC AACACACCGA CAGGGTGGAC ACATGAGCGT CGTGATCAGT ACTTCGTTTG 
0421 GGCCAAAAAG GTGGTGGACA ATCTGCGAGG AACCAACGCC AACCTGGAGC TCAAGCTGGA 
0481 CGAGATCTTC CGCCANCNCG

in silico PCR Fragment

0001 GGAGTGCCTC CAATATGCAG CTTTTAAGCA CCGGCAGCAA CGTCGCAAGG ATCCTCAGGA 
0061 AACCCCCTAT GTGAACCATG TGATCAATGT GAGCACTATT CTCTCCGTGG AGGCCTGCAT 
0121 CACAGATGAG GGAGTCCTAA TGGCTGCACT TCTGCACGAT GTCGTGGAGG ATACGGACGC 
0181 ATCTTTCGAG GATGTTGAGA AACTCTTTGG ACCGGATGTT TGTGGACTAG TGAGGGAGGT 
0241 GACGGACGAC AAGTCCCTGG AGAAACAGGA GAGAAAACGC CTGCAAATAG AAAATGCAGC 
0301 CAAAAGCAGC TGCAGAGCAA AGCTCATAAA GCTGGCTGAC AAATTGGATA ACCTAAGGGA 
0361 TCTGCAAGTC AACACACCGA CAGGGTGGAC ACAGGAGCGT CGTGATCAGT ACTTCGTTTG 
0421 GGCCAAAAAG GTGGTGGACA ATCTGCGAGG AACCAACGCC AACCTGGAGC TCAAGCTGGA 
0481 CGAGATCTTC CGCCAACGCG

Assemble Data

<PRE>

11900R-1.IR_full       1   GGAGTGCCTCCAATATGCAGCTTTTAAGCACCGGCAGCAACGTCGCAAGGATCCTCAGGA 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11900R-1.in silico     1   GGAGTGCCTCCAATATGCAGCTTTTAAGCACCGGCAGCAACGTCGCAAGGATCCTCAGGA 60


11900R-1.IR_full       61  AACCCCCTATGTGAACCATGTGATCAATGTGAGCACTATTCTCTCCGTGGAGGCCTGCAT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11900R-1.in silico     61  AACCCCCTATGTGAACCATGTGATCAATGTGAGCACTATTCTCTCCGTGGAGGCCTGCAT 120


11900R-1.IR_full       121 CACAGATGAGGGAGTCCTAATGGCTGCACTTCTGCACGATGTCGTGGAGGATACGGACGC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11900R-1.in silico     121 CACAGATGAGGGAGTCCTAATGGCTGCACTTCTGCACGATGTCGTGGAGGATACGGACGC 180


11900R-1.IR_full       181 ATCTTTCGAGGATGTTGAGAAACTCTTTGGACCGGATGTTTGTGGACTAGTGAGGGAGGT 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11900R-1.in silico     181 ATCTTTCGAGGATGTTGAGAAACTCTTTGGACCGGATGTTTGTGGACTAGTGAGGGAGGT 240


11900R-1.IR_full       241 GACGGACGACAAGTCCCTGGAGAAACAGGAGAGAAAACGCCTGCAAATAGAAAATGCAGC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11900R-1.in silico     241 GACGGACGACAAGTCCCTGGAGAAACAGGAGAGAAAACGCCTGCAAATAGAAAATGCAGC 300


11900R-1.IR_full       301 CAAAAGCAGCTGCAGAGCAAAGCTCATAAAGCTGGCTGACAAATTGGATAACCTAAGGGA 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11900R-1.in silico     301 CAAAAGCAGCTGCAGAGCAAAGCTCATAAAGCTGGCTGACAAATTGGATAACCTAAGGGA 360


11900R-1.IR_full       361 TCTGCAAGTCAACACACCGACAGGGTGGACACA<span class="snp"><tt>T</tt></span>GAGCGTCGTGATCAGTACTTCGTTTG 420
                           |||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||
11900R-1.in silico     361 TCTGCAAGTCAACACACCGACAGGGTGGACACA<span class="snp"><tt>G</tt></span>GAGCGTCGTGATCAGTACTTCGTTTG 420


11900R-1.IR_full       421 GGCCAAAAAGGTGGTGGACAATCTGCGAGGAACCAACGCCAACCTGGAGCTCAAGCTGGA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11900R-1.in silico     421 GGCCAAAAAGGTGGTGGACAATCTGCGAGGAACCAACGCCAACCTGGAGCTCAAGCTGGA 480


11900R-1.IR_full       481 CGAGATCTTCCGCCA<span class="snp"><tt>N</tt></span>C<span class="snp"><tt>N</tt></span>CG 500
                           |||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|<span class="snp"><tt>&nbsp;</tt></span>||
11900R-1.in silico     481 CGAGATCTTCCGCCA<span class="snp"><tt>A</tt></span>C<span class="snp"><tt>G</tt></span>CG 500

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_143425.1	CG11900-RA (CG11900), mRNA
0	0	3	2	4	NM_143338.2	CG12259-RA (CG12259), mRNA
0	0	2	5	2	NM_079097.1	CG9820-RA (Or59a), mRNA
0	0	2	4	3	NM_137212.2	CG30089-RA (CG30089), mRNA
0	0	2	2	4	NM_143459.1	CG1964-RA (Kul), mRNA
0	0	2	2	3	NM_133085.2	CG6606-RA (l(1)G0003), mRNA
0	0	2	2	3	NM_142930.2	CG10198-RA (Nup98), mRNA
0	0	1	4	11	NM_135068.2	CG10833-RA (Cyp28d1), mRNA
0	0	1	4	6	NM_169115.1	CG2017-RB, transcript variant B (CG2017), mRNA
0	0	1	4	6	NM_169116.1	CG2017-RC, transcript variant C (CG2017), mRNA
0	0	1	4	6	NM_169117.1	CG2017-RD, transcript variant D (CG2017), mRNA
0	0	1	4	6	NM_141346.2	CG2017-RA, transcript variant A (CG2017), mRNA
0	0	1	3	5	NM_166149.1	CG30094-RA (CG30094), mRNA
0	0	1	3	3	NM_166285.2	CG5174-RI, transcript variant I (CG5174), mRNA
0	0	1	3	3	NM_137487.3	CG5174-RA, transcript variant A (CG5174), mRNA
0	0	1	3	3	NM_166286.2	CG5174-RH, transcript variant H (CG5174), mRNA
0	0	1	3	3	NM_166283.1	CG5174-RJ, transcript variant J (CG5174), mRNA
0	0	1	3	3	NM_166287.1	CG5174-RK, transcript variant K (CG5174), mRNA
0	0	1	3	3	NM_166284.1	CG5174-RG, transcript variant G (CG5174), mRNA
0	0	1	3	3	NM_166288.2	CG5174-RB, transcript variant B (CG5174), mRNA
0	0	1	3	2	NM_137185.3	CG8155-RA (CG8155), mRNA
0	0	1	3	2	NM_167389.1	CG32613-RA (CG32613), mRNA
0	0	1	2	5	NM_141378.2	CG1155-RA (Osi14), mRNA
0	0	1	2	3	NM_132244.1	CG15344-RA (CG15344), mRNA
0	0	1	2	2	NM_165552.2	CG18812-RC, transcript variant C (CG18812), mRNA
0	0	1	2	2	NM_168861.1	CG32425-RE, transcript variant E (CG32425), mRNA
0	0	1	2	2	NM_167544.1	CG9623-RA, transcript variant A (if), mRNA
0	0	1	2	2	NM_078654.2	CG9623-RB, transcript variant B (if), mRNA
0	0	0	7	4	NM_135641.2	CG4705-RA, transcript variant A (CG4705), mRNA
0	0	0	7	4	NM_205960.1	CG4705-RB, transcript variant B (CG4705), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

dme00230 : Purine metabolism
dme01100 : Metabolic pathways

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