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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

11864R-4

Symbol

CG11864

Full Name

CG11864

CG No

CG11864

Old CG No

CG11864

Synonyms

BACR44L22.8, BG:BACR44L22.8, CG11864

Accession No (Link to NCBI)

NM_135912.2

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCGCAGATATGGGGTGTGATTT

Primer Seq. 3'

aatctagaggtaccCCACTGGATGCTGACGTATTG

Predicted Fragment Size

IR fragment full Seq

0001 CGCAGATATG GGGTGTGATT TTTTTGTTCA CTCCTACTGT TTTTAGTGAA CTCTTTAAGG 
0061 ATCCGGAGCT GCTTGCCGGA TTTTATCAGG GGGACATAAA AGCTCATCCA ATTCGTACTC 
0121 GAAACGGAAT TGTCAATCAG ATTTATCACT GGCCCAATCG CACTGTACCA TATATGATTG 
0181 AAGATGATGC ATTTGCCGAC AGTCATTATC GGGAAATTCT TAGAGCCATT TCCATCATAG 
0241 AGGAAAACTC ATGCGTTATT TTCAAGCCAG CTACTGAAAT GGATTTTCCC ATGGCGCTCG 
0301 TCATTACGTC AAAAGGTTTG GGCTGCAATA CCGTTCACTT GGGCTATAGG AATAAGACAC 
0361 AGGTGGTAAA TCTGGAGATT TACCCACTTG GCGAAGGCTG CTTTCGCATC GGTTCCATTA 
0421 TCCACGAGCT CCTTCACGTT TTGGGCTTCG AACATCAGCA TGTGTCCCAA AATCGGGACC 
0481 AATACGTCAG CATCCAGTGG

in silico PCR Fragment

0001 CGCAGATATG GGGTGTGATT TTTTTGTTCA CTCCTACTGT TTTTAGTGAA CTCTTTAAGG 
0061 ATCCGGAGCT GCTTGCCGGA TTTTATCAGG GGGACATAAA AGCTCATCCA ATTCGTACTC 
0121 GAAACGGAAT TGTCAATCAG ATTTATCACT GGCCCAATCG CACTGTACCA TATATGATTG 
0181 AAGATGATGC ATTTGCCGAC AGTCATTATC GGGAAATTCT TAGAGCCATT TCCATCATAG 
0241 AGGAAAACTC ATGCGTTATT TTCAAGCCAG CTACTGAAAT GGATTTTCCC ATGGCGCTCG 
0301 TCATTACGTC AAAAGGTTTG GGCTGCAATA CCGTTCACTT GGGCTATAGG AATAAGACAC 
0361 AGGTGGTAAA TCTGGAGATT TACCCACTTG GCGAAGGCTG CTTTCGCATC GGTTCCATTA 
0421 TCCACGAGCT CCTTCACGTT TTGGGCTTCG AACATCAGCA TGTGTCCCAA AATCGGGACC 
0481 AATACGTCAG CATCCAGTGG

Assemble Data


11864R-4.IR_full       1   CGCAGATATGGGGTGTGATTTTTTTGTTCACTCCTACTGTTTTTAGTGAACTCTTTAAGG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11864R-4.in silico     1   CGCAGATATGGGGTGTGATTTTTTTGTTCACTCCTACTGTTTTTAGTGAACTCTTTAAGG 60


11864R-4.IR_full       61  ATCCGGAGCTGCTTGCCGGATTTTATCAGGGGGACATAAAAGCTCATCCAATTCGTACTC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11864R-4.in silico     61  ATCCGGAGCTGCTTGCCGGATTTTATCAGGGGGACATAAAAGCTCATCCAATTCGTACTC 120


11864R-4.IR_full       121 GAAACGGAATTGTCAATCAGATTTATCACTGGCCCAATCGCACTGTACCATATATGATTG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11864R-4.in silico     121 GAAACGGAATTGTCAATCAGATTTATCACTGGCCCAATCGCACTGTACCATATATGATTG 180


11864R-4.IR_full       181 AAGATGATGCATTTGCCGACAGTCATTATCGGGAAATTCTTAGAGCCATTTCCATCATAG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11864R-4.in silico     181 AAGATGATGCATTTGCCGACAGTCATTATCGGGAAATTCTTAGAGCCATTTCCATCATAG 240


11864R-4.IR_full       241 AGGAAAACTCATGCGTTATTTTCAAGCCAGCTACTGAAATGGATTTTCCCATGGCGCTCG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11864R-4.in silico     241 AGGAAAACTCATGCGTTATTTTCAAGCCAGCTACTGAAATGGATTTTCCCATGGCGCTCG 300


11864R-4.IR_full       301 TCATTACGTCAAAAGGTTTGGGCTGCAATACCGTTCACTTGGGCTATAGGAATAAGACAC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11864R-4.in silico     301 TCATTACGTCAAAAGGTTTGGGCTGCAATACCGTTCACTTGGGCTATAGGAATAAGACAC 360


11864R-4.IR_full       361 AGGTGGTAAATCTGGAGATTTACCCACTTGGCGAAGGCTGCTTTCGCATCGGTTCCATTA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11864R-4.in silico     361 AGGTGGTAAATCTGGAGATTTACCCACTTGGCGAAGGCTGCTTTCGCATCGGTTCCATTA 420


11864R-4.IR_full       421 TCCACGAGCTCCTTCACGTTTTGGGCTTCGAACATCAGCATGTGTCCCAAAATCGGGACC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11864R-4.in silico     421 TCCACGAGCTCCTTCACGTTTTGGGCTTCGAACATCAGCATGTGTCCCAAAATCGGGACC 480


11864R-4.IR_full       481 AATACGTCAGCATCCAGTGG 500
                           ||||||||||||||||||||
11864R-4.in silico     481 AATACGTCAGCATCCAGTGG 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	2	2	NM_135912.2	CG11864-RA (CG11864), mRNA
0	0	3	2	2	NM_135455.2	CG4389-RA, transcript variant A (CG4389), mRNA
0	0	3	2	2	NM_164860.1	CG4389-RB, transcript variant B (CG4389), mRNA
0	0	3	2	2	NM_164861.1	CG4389-RC, transcript variant C (CG4389), mRNA
0	0	2	8	4	NM_001032051.1	CG33715-RD, transcript variant D (Msp-300), mRNA
0	0	1	5	8	NM_141738.3	CG6303-RA (Bruce), mRNA
0	0	1	3	6	NM_079205.3	CG7507-RA, transcript variant A (Dhc64C), mRNA
0	0	1	3	6	NM_168103.1	CG7507-RB, transcript variant B (Dhc64C), mRNA
0	0	1	3	4	NM_134311.2	CG9741-RC, transcript variant C (Dhod), mRNA
0	0	1	3	4	NM_057876.3	CG9741-RA, transcript variant A (Dhod), mRNA
0	0	1	2	7	NM_140809.2	CG3945-RA (Rad9), mRNA
0	0	1	2	5	NM_164998.1	CG31860-RA (CG31860), mRNA
0	0	1	2	2	NM_168784.1	CG32204-RA (CG32204), mRNA
0	0	0	5	4	NM_130643.2	CG2854-RA (CG2854), mRNA
0	0	0	5	3	NM_001014583.1	CG32134-RB, transcript variant B (btl), mRNA
0	0	0	5	3	NM_168577.2	CG32134-RA, transcript variant A (btl), mRNA
0	0	0	4	6	NM_136212.1	CG9323-RA (CG9323), mRNA
0	0	0	4	5	NM_139635.1	CG15014-RA (CG15014), mRNA
0	0	0	3	3	NM_137046.3	CG6191-RA (CG6191), mRNA
0	0	0	3	3	NM_079384.2	CG4314-RA (st), mRNA
0	0	0	3	3	NM_001032103.1	CG33678-RA (CG33678), mRNA
0	0	0	3	2	NM_169751.1	CG31267-RA (CG31267), mRNA
0	0	0	2	56	NM_168226.1	CG32377-RA (CG32377), mRNA
0	0	0	2	11	NM_135911.1	CG15255-RA (CG15255), mRNA
0	0	0	2	7	NM_138251.2	CG2277-RA (CG2277), mRNA
0	0	0	2	7	NM_078521.2	CG32858-RC, transcript variant C (sn), mRNA
0	0	0	2	7	NM_167142.1	CG32858-RA, transcript variant A (sn), mRNA
0	0	0	2	7	NM_167143.1	CG32858-RB, transcript variant B (sn), mRNA
0	0	0	2	6	NM_138031.1	CG3173-RA (CG3173), mRNA
0	0	0	2	6	NM_139943.1	CG7201-RA (CG7201), mRNA

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