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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

11661R-2

Symbol

Nc73EF

Full Name

Neural conserved at 73EF

CG No

CG11661

Old CG No

CG11661

Synonyms

141810_at, FBgn0036682, CG11661, anon-WO0118547.329, Nc73EF, Neural conserved at 73EF

Accession No (Link to NCBI)

NM_176340.1

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGTCCAGGCCATCATCAGGAGC

Primer Seq. 3'

aatctagaggtaccCCCAACTCCGTTGATACGTCG

Predicted Fragment Size

IR fragment full Seq

0001 GTCCAGGCCA TCATCAGGAG CTACCAGTCA CGAGGTCATA ATATTGGCAT CTGATCTGGA 
0061 TCCGCTTGGA ATTTTGACTC GCGAAAAAAC CGTCTGCAAG GATGGGCTAG CACGTCGTCG 
0121 TAACGAAGAT GTGCTCAGGC AGCACTCTGG GTTTTTGTTT GGCGAGCAGG ACATGGATCG 
0181 CCAGTTCAAG CTGCCCAGCA CTACCTTCAT CGGAGGCGAT GAGGCTTCGT TGCCCCTAAA 
0241 GGAAATCCTG AACCGCCTGG AGAATGTCTA CTGTAATAAG ATTGGCGTGG GGTTCATGTT 
0301 CATCAACTCT CTGGAGCAAT GCAACTGGAT CCGCAAGCGT TTTGAGACCC CCGGCGTCCT 
0361 TAACTTCTCG CCCGAGGAGA AGCGTCTGAT CCTGGCCCGT TTGACCCGTG CCACCGGCTT 
0421 TGAGGCTTTC CTGGCCAAGA AGTACTCTTC TGAGAAACGT TTCGGTCTGG AGGGTTGCGA 
0481 GATCATGATC CCAGCCCTGA AGGAGATCAT CGACGTATCA ACGGAGTTGG G

in silico PCR Fragment

0001 GTCCAGGCCA TCATCAGGAG CTACCAGTCA CGAGGTCAT- ---TTGGCAT CTGATCTGGA 
0061 TCCGCTTGGA ATTTTGACTC GCGAAAAAAC CGTCTGCAAG GATGGGCTAG CACGTCGTGC 
0121 TAACGAAGAT GTGCTCAGGC AGCACTCTGG GTTTTTGTTT GGCGAGCAGG ACATGGATCG 
0181 CCAGTTCAAG CTGCCCAGCA CTACCTTCAT CGGAGGCGAT GAGGCTTCGT TGCCCCTAAA 
0241 GGAAATCCTG AACCGCCTGG AGAATGTCTA CTGTAATAAG ATTGGCGTGG AGTTCATGTT 
0301 CATCAACTCT CTGGAGCAAT GCAACTGGAT CCGCAAGCGT TTTGAGACCC CCGGCGTCCT 
0361 TAACTTCTCG CCCGAGGAGA AGCGTCTGAT CCTGGCCCGT TTGACCCGTG CCACCGGCTT 
0421 TGAGGCTTTC CTGGCCAAGA AGTACTCTTC TGAGAAACGT TTCGGTCTGG AGGGTTGCGA 
0481 GATCATGATC CCAGCCCTGA AGGAGATCAT CGACGTATCA ACGGAGTTGG G

Assemble Data

<PRE>

11661R-2.IR_full       1   GTCCAGGCCATCATCAGGAGCTACCAGTCACGAGGTCAT<span class="snp"><tt>A</tt></span><span class="snp"><tt>A</tt></span><span class="snp"><tt>T</tt></span><span class="snp"><tt>A</tt></span>TTGGCATCTGATCTGGA 60
                           |||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||
11661R-2.in silico     1   GTCCAGGCCATCATCAGGAGCTACCAGTCACGAGGTCAT<span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span>TTGGCATCTGATCTGGA 60


11661R-2.IR_full       61  TCCGCTTGGAATTTTGACTCGCGAAAAAACCGTCTGCAAGGATGGGCTAGCACGTCGT<span class="snp"><tt>C</tt></span><span class="snp"><tt>G</tt></span> 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>
11661R-2.in silico     61  TCCGCTTGGAATTTTGACTCGCGAAAAAACCGTCTGCAAGGATGGGCTAGCACGTCGT<span class="snp"><tt>G</tt></span><span class="snp"><tt>C</tt></span> 120


11661R-2.IR_full       121 TAACGAAGATGTGCTCAGGCAGCACTCTGGGTTTTTGTTTGGCGAGCAGGACATGGATCG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11661R-2.in silico     121 TAACGAAGATGTGCTCAGGCAGCACTCTGGGTTTTTGTTTGGCGAGCAGGACATGGATCG 180


11661R-2.IR_full       181 CCAGTTCAAGCTGCCCAGCACTACCTTCATCGGAGGCGATGAGGCTTCGTTGCCCCTAAA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11661R-2.in silico     181 CCAGTTCAAGCTGCCCAGCACTACCTTCATCGGAGGCGATGAGGCTTCGTTGCCCCTAAA 240


11661R-2.IR_full       241 GGAAATCCTGAACCGCCTGGAGAATGTCTACTGTAATAAGATTGGCGTGG<span class="snp"><tt>G</tt></span>GTTCATGTT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||
11661R-2.in silico     241 GGAAATCCTGAACCGCCTGGAGAATGTCTACTGTAATAAGATTGGCGTGG<span class="snp"><tt>A</tt></span>GTTCATGTT 300


11661R-2.IR_full       301 CATCAACTCTCTGGAGCAATGCAACTGGATCCGCAAGCGTTTTGAGACCCCCGGCGTCCT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11661R-2.in silico     301 CATCAACTCTCTGGAGCAATGCAACTGGATCCGCAAGCGTTTTGAGACCCCCGGCGTCCT 360


11661R-2.IR_full       361 TAACTTCTCGCCCGAGGAGAAGCGTCTGATCCTGGCCCGTTTGACCCGTGCCACCGGCTT 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11661R-2.in silico     361 TAACTTCTCGCCCGAGGAGAAGCGTCTGATCCTGGCCCGTTTGACCCGTGCCACCGGCTT 420


11661R-2.IR_full       421 TGAGGCTTTCCTGGCCAAGAAGTACTCTTCTGAGAAACGTTTCGGTCTGGAGGGTTGCGA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11661R-2.in silico     421 TGAGGCTTTCCTGGCCAAGAAGTACTCTTCTGAGAAACGTTTCGGTCTGGAGGGTTGCGA 480


11661R-2.IR_full       481 GATCATGATCCCAGCCCTGAAGGAGATCATCGACGTATCAACGGAGTTGGG 531
                           |||||||||||||||||||||||||||||||||||||||||||||||||||
11661R-2.in silico     481 GATCATGATCCCAGCCCTGAAGGAGATCATCGACGTATCAACGGAGTTGGG 531

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	3	NM_168703.2	CG11661-RC, transcript variant C (Nc73EF), mRNA
100	482	0	0	3	NM_176341.1	CG11661-RG, transcript variant G (Nc73EF), mRNA
100	482	0	0	3	NM_168702.1	CG11661-RB, transcript variant B (Nc73EF), mRNA
100	482	0	0	3	NM_168701.2	CG11661-RA, transcript variant A (Nc73EF), mRNA
100	482	0	0	3	NM_176342.1	CG11661-RH, transcript variant H (Nc73EF), mRNA
75.72	365	2	2	10	NM_176340.1	CG11661-RF, transcript variant F (Nc73EF), mRNA
0.62	3	3	2	2	NM_140258.2	CG7264-RA (CG7264), mRNA
0	0	3	2	3	NM_132220.2	CG2263-RA (CG2263), mRNA
0	0	2	2	2	NM_079745.2	CG5289-RA (Pros26.4), mRNA
0	0	2	1	1	NM_135441.1	CG3752-RA (Aldh), mRNA
0	0	1	7	7	NM_164692.2	CG31638-RA (CG31638), mRNA
0	0	1	5	3	NM_132946.2	CG9104-RA (CG9104), mRNA
0	0	1	5	3	NM_132945.2	CG4872-RA (CG4872), mRNA
0	0	1	3	4	NM_131933.1	CG12693-RB (CG12693), mRNA
0	0	1	3	3	NM_169594.2	CG3389-RA (Cad88C), mRNA
0	0	1	3	3	NM_137235.2	CG8399-RA (CG8399), mRNA
0	0	1	2	6	NM_166131.1	CG8322-RB, transcript variant B (ATPCL), mRNA
0	0	1	2	5	NM_143759.2	CG6760-RA (l(3)70Da), mRNA
0	0	1	2	5	NM_170493.1	CG31020-RA (spdo), mRNA
0	0	1	2	5	NM_079031.1	CG8322-RA, transcript variant A (ATPCL), mRNA
0	0	1	2	4	NM_135371.3	CG7627-RA (CG7627), mRNA
0	0	1	2	3	NM_078676.3	CG6223-RA (betaCop), mRNA
0	0	1	2	2	NM_140465.2	CG9311-RA (CG9311), mRNA
0	0	1	2	2	NM_206157.1	CG6446-RB, transcript variant B (Sema-1b), mRNA
0	0	1	2	2	NM_137400.2	CG6446-RA, transcript variant A (Sema-1b), mRNA
0	0	0	10	25	NM_001031931.1	CG33791-RD, transcript variant D (CG33791), mRNA
0	0	0	10	25	NM_001031929.1	CG33791-RB, transcript variant B (CG33791), mRNA
0	0	0	10	25	NM_001031930.1	CG33791-RA, transcript variant A (CG33791), mRNA
0	0	0	10	25	NM_001031928.1	CG33791-RC, transcript variant C (CG33791), mRNA
0	0	0	10	25	NM_001031926.1	CG18170-RB, transcript variant B (CG18170), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

dme00020 : Citrate cycle (TCA cycle)
dme00785 : Lipoic acid metabolism
dme01100 : Metabolic pathways
dme01200 : Carbon metabolism
dme01210 : 2-Oxocarboxylic acid metabolism

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