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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
11621R-1 |
| Symbol |
Pi3K68D |
Full Name |
Phosphotidylinositol 3 kinase 68D |
| CG No |
CG11621 |
Old CG No |
CG11621 |
| Synonyms |
PI3K, dPI3K, PI3K_68D, CG11621, PI(3)K, cpk, PI3K 68D, PI3K-68D/E, Cpk, PI3K 68_D, PI3K-68D, dPIK, Pi3K, BcDNA:LD15217, Pi3K68D |
| Accession No (Link to NCBI) |
NM_079304.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Ohhara Y, Hoshino G, Imahori K, Matsuyuki T, Yamakawa-Kobayashi K.<br> The Nutrient-Responsive Molecular Chaperone Hsp90 Supports Growth and Development in <i>Drosophila</i>.<br> Front Physiol (2021) 12 690564
[
PubMed ID = 34239451
]
[
RRC reference
]
Perkins AD, Lee MJ, Tanentzapf G.<br> The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance.<br> Sci Data (2014) 1 140002
[
PubMed ID = 25977760
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, Dürrenberger M, Paro R, Boutros M, Merdes G.<br> A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.<br> Dev Cell (2010) 18(5) 862-76
[
PubMed ID = 20493818
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgAGAGCAGCATGCCCTCGATGA |
| Primer Seq. 3' |
aatctagaggtaccGCTGAGCAGCTGGTTGGTAAG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 AGAGCAGCAT NNCCTCGATG ACTACAGGCG AAACAAGAAG TACGGCTCCG GGTATCAGCA
0061 AANCTCCACC GTTGCTGGCC GAGATTACCA GGCGGCGCAA CGTAGTNCAG AGCCTACATC
0121 AACCACGACG GCACTCGGAG GTGCATCAGG TGGCCATCAG TCCGGAGAAT GCGGAACGAT
0181 TCGCGCACAC CGCCGGCCCA GGGAACGGAT AACGATCTGA TACTGCTTCG CAAGTCCCAC
0241 CAGCAAGCAG CCAGAGAGTA GCAGTCCCTT TGGCAAACTT ATAGAGGATC TGCAGCGGAT
0301 GCAGCCGACC AATCCGCAGT CGGCCCTGGT GCCAATGGGT CCAGTTGCGT CGGCTTCGAT
0361 TCCTCCTCAA TACGGNCTTT CCACCTCATC AGCAACGTCC AACGGCTGCT CAGCCCACAC
0421 CGTACGGCAT GGTTGCAGGT GGAGTTGTTG GTGGACCGGC TTACGGTGAC CTGCAGTTGG
0481 TGCCTTACCA ACCAGCTGCT CAGC |
| in silico PCR Fragment |
0001 AGAGCAGCAT GCCCTCGATG ACTACAGGCG AAACAAGAAG TACGGCTCCG GGTATCAGCA
0061 AAGCTCCACC GTTGCTGGCC GAGATTACCA GGCGGCGCAA CGTAGT-CAG AGCCTACATC
0121 AACCACGACG GCACTCGGAG GTGCATCAGG TGGCCATCAG TCCGGAGAAT GCGGAACGA-
0181 TCGCGCACAC CGCCGGCCCA GGGAACGGAT AACGATCTGA T-CTGCTTCG CAAGTCCCAC
0241 CAGCAAGCAG CCAGAGAGTA GCAGTCCCTT TGGCAAACTT ATAGAGGATC TGCAGCGGAT
0301 GCAGCCGACC AATCCGCAGT CGGCCCTGGT GCCAATGGGT CCAGTTGCGT CGGCTTCGAT
0361 TCCTCCTCAA TACGG-CTTT CCACCTCATC AGCAACGTCC AACGGCTGCT CAGCCCACAC
0421 CGTACGGCAT GGTTGCAGGT GGAGTTGTTG GTGGACCGGC TTACGGTGAC CTGCAGTTGG
0481 TGCCTTACCA ACCAGCTGCT CAGC |
| Assemble Data |
<PRE>
11621R-1.IR_full 1 AGAGCAGCAT<span class="snp"><tt>N</tt></span><span class="snp"><tt>N</tt></span>CCTCGATGACTACAGGCGAAACAAGAAGTACGGCTCCGGGTATCAGCA 60
||||||||||<span class="snp"><tt> </tt></span><span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||||||||||||
11621R-1.in silico 1 AGAGCAGCAT<span class="snp"><tt>G</tt></span><span class="snp"><tt>C</tt></span>CCTCGATGACTACAGGCGAAACAAGAAGTACGGCTCCGGGTATCAGCA 60
11621R-1.IR_full 61 AA<span class="snp"><tt>N</tt></span>CTCCACCGTTGCTGGCCGAGATTACCAGGCGGCGCAACGTAGT<span class="snp"><tt>N</tt></span>CAGAGCCTACATC 120
||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||
11621R-1.in silico 61 AA<span class="snp"><tt>G</tt></span>CTCCACCGTTGCTGGCCGAGATTACCAGGCGGCGCAACGTAGT<span class="snp"><tt>-</tt></span>CAGAGCCTACATC 120
11621R-1.IR_full 121 AACCACGACGGCACTCGGAGGTGCATCAGGTGGCCATCAGTCCGGAGAATGCGGAACGA<span class="snp"><tt>T</tt></span> 180
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>
11621R-1.in silico 121 AACCACGACGGCACTCGGAGGTGCATCAGGTGGCCATCAGTCCGGAGAATGCGGAACGA<span class="snp"><tt>-</tt></span> 180
11621R-1.IR_full 181 TCGCGCACACCGCCGGCCCAGGGAACGGATAACGATCTGAT<span class="snp"><tt>A</tt></span>CTGCTTCGCAAGTCCCAC 240
|||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||
11621R-1.in silico 181 TCGCGCACACCGCCGGCCCAGGGAACGGATAACGATCTGAT<span class="snp"><tt>-</tt></span>CTGCTTCGCAAGTCCCAC 240
11621R-1.IR_full 241 CAGCAAGCAGCCAGAGAGTAGCAGTCCCTTTGGCAAACTTATAGAGGATCTGCAGCGGAT 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11621R-1.in silico 241 CAGCAAGCAGCCAGAGAGTAGCAGTCCCTTTGGCAAACTTATAGAGGATCTGCAGCGGAT 300
11621R-1.IR_full 301 GCAGCCGACCAATCCGCAGTCGGCCCTGGTGCCAATGGGTCCAGTTGCGTCGGCTTCGAT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11621R-1.in silico 301 GCAGCCGACCAATCCGCAGTCGGCCCTGGTGCCAATGGGTCCAGTTGCGTCGGCTTCGAT 360
11621R-1.IR_full 361 TCCTCCTCAATACGG<span class="snp"><tt>N</tt></span>CTTTCCACCTCATCAGCAACGTCCAACGGCTGCTCAGCCCACAC 420
|||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||||||||
11621R-1.in silico 361 TCCTCCTCAATACGG<span class="snp"><tt>-</tt></span>CTTTCCACCTCATCAGCAACGTCCAACGGCTGCTCAGCCCACAC 420
11621R-1.IR_full 421 CGTACGGCATGGTTGCAGGTGGAGTTGTTGGTGGACCGGCTTACGGTGACCTGCAGTTGG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11621R-1.in silico 421 CGTACGGCATGGTTGCAGGTGGAGTTGTTGGTGGACCGGCTTACGGTGACCTGCAGTTGG 480
11621R-1.IR_full 481 TGCCTTACCAACCAGCTGCTCAGC 504
||||||||||||||||||||||||
11621R-1.in silico 481 TGCCTTACCAACCAGCTGCTCAGC 504
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
3 |
NM_168475.1 |
CG11621-RC, transcript variant C (Pi3K68D), mRNA |
| 100 |
482 |
0 |
0 |
3 |
NM_079304.2 |
CG11621-RA, transcript variant A (Pi3K68D), mRNA |
| 100 |
482 |
0 |
0 |
3 |
NM_168476.1 |
CG11621-RB, transcript variant B (Pi3K68D), mRNA |
| 0 |
0 |
4 |
2 |
6 |
NM_132591.3 |
CG17762-RB, transcript variant B (tomosyn), mRNA |
| 0 |
0 |
4 |
2 |
5 |
NM_136966.1 |
CG8771-RA (CG8771), mRNA |
| 0 |
0 |
4 |
2 |
4 |
NM_167329.2 |
CG17762-RC, transcript variant C (tomosyn), mRNA |
| 0 |
0 |
4 |
2 |
2 |
NM_170319.1 |
CG31067-RA (CG31067), mRNA |
| 0 |
0 |
4 |
2 |
2 |
NM_167330.2 |
CG17762-RD, transcript variant D (tomosyn), mRNA |
| 0 |
0 |
4 |
2 |
2 |
NM_167328.2 |
CG17762-RA, transcript variant A (tomosyn), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_168640.1 |
CG5942-RD, transcript variant D (brm), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_168641.1 |
CG5942-RB, transcript variant B (brm), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_080497.4 |
CG5942-RC, transcript variant C (brm), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_080498.2 |
CG5942-RA, transcript variant A (brm), mRNA |
| 0 |
0 |
2 |
2 |
6 |
NM_164375.1 |
CG18497-RC, transcript variant C (spen), mRNA |
| 0 |
0 |
2 |
2 |
6 |
NM_164374.1 |
CG18497-RA, transcript variant A (spen), mRNA |
| 0 |
0 |
2 |
2 |
6 |
NM_079979.2 |
CG18497-RB, transcript variant B (spen), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_206620.4 |
CG32782-RD, transcript variant D (tlk), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_130717.3 |
CG32782-RC, transcript variant C (tlk), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_136251.2 |
CG9246-RA (CG9246), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_001043286.1 |
CG6383-RB, transcript variant B (crb), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_079756.2 |
CG6383-RA, transcript variant A (crb), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_078687.1 |
CG12529-RA, transcript variant A (Zw), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_167676.1 |
CG12529-RB, transcript variant B (Zw), mRNA |
| 0 |
0 |
0 |
7 |
10 |
NM_001014630.1 |
CG33547-RA (Rim), mRNA |
| 0 |
0 |
0 |
7 |
5 |
NM_132595.1 |
CG15725-RA (CG15725), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_142902.2 |
CG16723-RA (CG16723), mRNA |
| 0 |
0 |
0 |
5 |
15 |
NM_137081.2 |
CG30483-RA (Prosap), mRNA |
| 0 |
0 |
0 |
5 |
8 |
NM_141474.2 |
CG2616-RA (CG2616), mRNA |
| 0 |
0 |
0 |
5 |
7 |
NM_136638.2 |
CG8799-RA (l(2)03659), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_132640.2 |
CG1770-RA, transcript variant A (HDAC4), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| +- |
|
| DB |
 |
| +- |
|
| DC |
 |
| +- |
|
| DM |
 |
| |
|
| VM |
 |
| |
|
| VB |
 |
| |
|
|
Caspase-3 signal
|
|
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Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
dme00562
:
Inositol phosphate metabolism
dme01100
:
Metabolic pathways
dme04070
:
Phosphatidylinositol signaling system
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|
