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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 11593R-2 
 Symbol CG11593  Full Name CG11593 
 CG No CG11593  Old CG No CG11593 
 Synonyms anon-EST:Posey64, CG11593 
 Accession No (Link to NCBI) NM_139594.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees semi-lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCGCCGGAGAAGAATATCCTGA 
 Primer Seq. 3' 
aatctagaggtaccAGGGAGTGCAGTCGATCGTTT 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CGCCGGAGAA GAATATCCTG AAATTGGAGC AGGCCAAGCA GGATCACGAT AAGGAGGAAC 
0061 TGGAGTTCAA GACGACCTCC GCGCTGGACA ACAGAATACT GCGTCCAGAG CTGCTGCCCA 
0121 TGGAGGCGAT GCCACAACGA CACAACATCA TCGAGCGCAC CCTGGCCAAC AGCCATCCGC 
0181 TGATGTCGCC AACCAACACG GACTCAGACT CGGAACCCTT TCAGCTGAAG AAGCAGGAGA 
0241 AACAGCTGCC GAGGAGCAAC TTTCCGGACG TTGTGCCCAC CACGGAGACG GTGCAGGTGA 
0301 AGAACAACCT GAACCAATGC CGGAACAAGC CGCAGGACGC CAAGGTCAGC CTGCTAAGGG 
0361 CCAACAGTCC GGATATCCTG AGCAGCGAGT CAGATGCGGA TGTGTCCCAG TACTTGGCCG 
0421 CCGTGGAGTC TAACTTTCAG TCCGTTTCCC TGATgNCCAA TGGCAATGGC AAGAAATCCA 
0481 AACGATCGAC TGCACTCCCT  
 in silico PCR Fragment 
0001 CGCCGGAGAA GAATATCCTG AAATTGGAGC AGGCCAAGCA GGATCACGAT AAGGAGGAAC 
0061 TGGAGTTCAA GACGACCTCC GCGCTGGACA ACAGAATACT GCGTCCAGAG CTGCTGCCCA 
0121 TGGAGGCGAT GCCACAACGA CACAACATCA TCGAGCGCAC CCTGGCCAAC AGCCATCCGC 
0181 TGATGTCGCC AACCAACACG GACTCAGACT CGGAACCCTT TCAGCTGAAG AAGCAGGAGA 
0241 AACAGCTGCC GAGGAGCAAC TTTCCGGACG TTGTGCCCAC CACGGAGACG GTGCAGGTGA 
0301 AGAACAACCT GAACCAATGC CGGAACAAGC CGCAGGACGC CAAGGTCAGC CTGCTAAGGG 
0361 CCAACAGTCC GGATATCCTG AGCAGCGAGT CAGATGCGGA TGTGTCCCAG TACTTGGCCG 
0421 CCGTGGAGTC TAACTTTCAG TCCGTTTCCC TGATGCCCAA TGGCAATGGC AAGAAATCCA 
0481 AACGATCGAC TGCACTCCCT  
 Assemble Data 
<PRE>

11593R-2.IR_full       1   CGCCGGAGAAGAATATCCTGAAATTGGAGCAGGCCAAGCAGGATCACGATAAGGAGGAAC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11593R-2.in silico     1   CGCCGGAGAAGAATATCCTGAAATTGGAGCAGGCCAAGCAGGATCACGATAAGGAGGAAC 60


11593R-2.IR_full       61  TGGAGTTCAAGACGACCTCCGCGCTGGACAACAGAATACTGCGTCCAGAGCTGCTGCCCA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11593R-2.in silico     61  TGGAGTTCAAGACGACCTCCGCGCTGGACAACAGAATACTGCGTCCAGAGCTGCTGCCCA 120


11593R-2.IR_full       121 TGGAGGCGATGCCACAACGACACAACATCATCGAGCGCACCCTGGCCAACAGCCATCCGC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11593R-2.in silico     121 TGGAGGCGATGCCACAACGACACAACATCATCGAGCGCACCCTGGCCAACAGCCATCCGC 180


11593R-2.IR_full       181 TGATGTCGCCAACCAACACGGACTCAGACTCGGAACCCTTTCAGCTGAAGAAGCAGGAGA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11593R-2.in silico     181 TGATGTCGCCAACCAACACGGACTCAGACTCGGAACCCTTTCAGCTGAAGAAGCAGGAGA 240


11593R-2.IR_full       241 AACAGCTGCCGAGGAGCAACTTTCCGGACGTTGTGCCCACCACGGAGACGGTGCAGGTGA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11593R-2.in silico     241 AACAGCTGCCGAGGAGCAACTTTCCGGACGTTGTGCCCACCACGGAGACGGTGCAGGTGA 300


11593R-2.IR_full       301 AGAACAACCTGAACCAATGCCGGAACAAGCCGCAGGACGCCAAGGTCAGCCTGCTAAGGG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11593R-2.in silico     301 AGAACAACCTGAACCAATGCCGGAACAAGCCGCAGGACGCCAAGGTCAGCCTGCTAAGGG 360


11593R-2.IR_full       361 CCAACAGTCCGGATATCCTGAGCAGCGAGTCAGATGCGGATGTGTCCCAGTACTTGGCCG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11593R-2.in silico     361 CCAACAGTCCGGATATCCTGAGCAGCGAGTCAGATGCGGATGTGTCCCAGTACTTGGCCG 420


11593R-2.IR_full       421 CCGTGGAGTCTAACTTTCAGTCCGTTTCCCTGATG<span class="snp"><tt>N</tt></span>CCAATGGCAATGGCAAGAAATCCA 480
                           |||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||
11593R-2.in silico     421 CCGTGGAGTCTAACTTTCAGTCCGTTTCCCTGATG<span class="snp"><tt>C</tt></span>CCAATGGCAATGGCAAGAAATCCA 480


11593R-2.IR_full       481 AACGATCGACTGCACTCCCT 500
                           ||||||||||||||||||||
11593R-2.in silico     481 AACGATCGACTGCACTCCCT 500

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  1  NM_139594.1  CG11593-RA (CG11593), mRNA 
0.2   1  4  3  5  NM_134474.4  CG32532-RA (CG32532), mRNA 
0   0  3  5  13  NM_078977.2  CG10897-RA, transcript variant A (tou), mRNA 
0   0  3  4  4  NM_142205.2  CG6130-RA (CG6130), mRNA 
0   0  3  4  3  NM_165820.1  CG13211-RB, transcript variant B (CG13211), mRNA 
0   0  3  4  3  NM_136824.2  CG13211-RA, transcript variant A (CG13211), mRNA 
0   0  3  2  4  NM_205877.1  CG32019-RD, transcript variant D (bt), mRNA 
0   0  3  2  4  NM_205876.1  CG32019-RC, transcript variant C (bt), mRNA 
0   0  3  2  4  NM_205875.1  CG32019-RE, transcript variant E (bt), mRNA 
0   0  3  2  4  NM_166790.2  CG32019-RA, transcript variant A (bt), mRNA 
0   0  3  2  3  NM_206335.1  CG7334-RB, transcript variant B (Sug), mRNA 
0   0  3  2  3  NM_140236.2  CG7334-RA, transcript variant A (Sug), mRNA 
0   0  3  2  3  NM_079976.3  CG17320-RA (ScpX), mRNA 
0   0  2  7  2  NM_139811.1  CG10063-RA (CG10063), mRNA 
0   0  2  3  3  NM_139388.1  CG13930-RA (CG13930), mRNA 
0   0  2  2  5  NM_170295.1  CG31078-RA (CG31078), mRNA 
0   0  2  2  3  NM_167233.1  CG32687-RA (CG32687), mRNA 
0   0  2  2  2  NM_132340.2  CG2998-RA (RpS28b), mRNA 
0   0  1  6  7  NM_138984.2  CG5712-RA (ACXD), mRNA 
0   0  1  5  9  NM_001014610.1  CG33546-RB, transcript variant B (gfzf), mRNA 
0   0  1  5  7  NM_134763.1  CG14351-RA (CG14351), mRNA 
0   0  1  5  6  NM_132660.2  CG1716-RA (CG1716), mRNA 
0   0  1  5  3  NM_143422.1  CG11898-RA (CG11898), mRNA 
0   0  1  4  5  NM_001014504.1  CG30372-RB (CG30372), mRNA 
0   0  1  4  4  NM_135993.2  CG15141-RA (CG15141), mRNA 
0   0  1  4  3  NM_142385.1  CG14322-RA (CG14322), mRNA 
0   0  1  4  2  NM_169996.1  CG5740-RA, transcript variant A (CG5740), mRNA 
0   0  1  4  2  NM_142753.2  CG5740-RB, transcript variant B (CG5740), mRNA 
0   0  1  3  7  NM_141657.2  CG8273-RA (CG8273), mRNA 
0   0  1  3  5  NM_057502.2  CG1322-RB, transcript variant B (zfh1), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway

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