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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
11561R-1 |
| Symbol |
smo |
Full Name |
smoothened |
| CG No |
CG11561 |
Old CG No |
CG11561 |
| Synonyms |
CG11561, Smo, dSmo, smo, SMO |
| Accession No (Link to NCBI) |
NM_078719.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Goins LM, Girard JR, Mondal BC, Buran S, Su CC, Tang R, Biswas T, Kissi JA, Banerjee U.<br> Wnt signaling couples G2 phase control with differentiation during hematopoiesis in Drosophila.<br> Dev Cell (2024)
[
PubMed ID = 38866012
]
[
RRC reference
]
Lai CM, Lin KY, Kao SH, Chen YN, Huang F, Hsu HJ.<br> Hedgehog signaling establishes precursors for germline stem cell niches by regulating cell adhesion.<br> J Cell Biol (2017) 216(5) 1439-1453
[
PubMed ID = 28363970
]
[
RRC reference
]
Zhang Z, Lv X, Jiang J, Zhang L, Zhao Y.<br> Dual roles of Hh signaling in the regulation of somatic stem cell self-renewal and germline stem cell maintenance in Drosophila testis.<br> Cell Res (2013) 23(4) 573-6
[
PubMed ID = 23419515
]
[
RRC reference
]
Han H, Pan C, Liu C, Lv X, Yang X, Xiong Y, Lu Y, Wu W, Han J, Zhou Z, Jiang H, Zhang L, Zhao Y.<br> Gut-neuron interaction via Hh signaling regulates intestinal progenitor cell differentiation in Drosophila.<br> Cell Discov (2015) 1 15006
[
PubMed ID = 27462407
]
[
RRC reference
]
Li Z, Guo Y, Han L, Zhang Y, Shi L, Huang X, Lin X.<br> Debra-mediated Ci degradation controls tissue homeostasis in Drosophila adult midgut.<br> Stem Cell Reports (2014) 2(2) 135-44
[
PubMed ID = 24527387
]
[
RRC reference
]
Yang X, Mao F, Lv X, Zhang Z, Fu L, Lu Y, Wu W, Zhou Z, Zhang L, Zhao Y.<br> Drosophila Vps36 regulates Smo trafficking in Hedgehog signaling.<br> J Cell Sci (2013) 126(Pt 18) 4230-8
[
PubMed ID = 23843610
]
[
RRC reference
]
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| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTTACCGACTGTACGCCAAGAA |
| Primer Seq. 3' |
aatctagaggtaccCTGTCGGAACCAAAGGACTTA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TTACCGACTG TACGCCAAGA AGGGCAGGGA CGACAAACCC TGGTTTGATG GCCTAGACAG
0061 CAGGCACATC CAGTGTGTCC GACGTGCCCG TTGCTACCCC ACCTCGAACG CAACCAACAC
0121 CTGTTTCGGC TCAAAATTGG CCTATGAGCT GAGCAGCCTA GATCTCACCG ACTTCCACAC
0181 CGAAAAGGAG CTGAACGATA AGCTGAACGA CTACTATGCC CTGAAGCACG TGCCCAAATG
0241 TTGGGCAGCT ATACAGCCCT TTTTGTGCGC CGTCTTTAAG CCGAAGTGTG AAAAAATCAA
0301 CGGCGAGGAC ATGGTCTACC TGCCATCTTA CGAGATGTGC CGAATTACCA TGGAACCCTG
0361 TCGCATTTTG TACAACACGA CGTTTTTCCC AAAATTCCTT CGCTGCAACG AAACACTCTT
0421 TCCGACGAAA TGCACAAACG GAGCACGAGG AATGAAATTC AACGGAACTG GCCAGTGTTT
0481 AAGTCCTTTG GTTCCGACAG |
| in silico PCR Fragment |
0001 TTACCGACTG TACGCCAAGA AGGGCAGGGA CGACAAACCC TGGTTTGATG GCCTAGACAG
0061 CAGGCACATC CAGTGTGTCC GACGTGCCCG TTGCTACCCC ACCTCGAACG CAACCAACAC
0121 CTGTTTCGGC TCAAAATTGC CCTATGAGCT GAGCAGCCTA GATCTCACCG ACTTCCACAC
0181 CGAAAAGGAG CTGAACGATA AGCTGAACGA CTACTATGCC CTGAAGCACG TGCCCAAATG
0241 TTGGGCAGCT ATACAGCCCT TTTTGTGCGC CGTCTTTAAG CCGAAGTGTG AAAAAATCAA
0301 CGGCGAGGAC ATGGTCTACC TGCCATCTTA CGAGATGTGC CGAATTACCA TGGAACCCTG
0361 TCGCATTTTG TACAACACGA CGTTTTTCCC AAAATTCCTT CGCTGCAACG AAACACTCTT
0421 TCCGACGAAA TGCACAAACG GAGCACGAGG AATGAAATTC AACGGAACTG GCCAGTGTTT
0481 AAGTCCTTTG GTTCCGACAG |
| Assemble Data |
<PRE>
11561R-1.IR_full 1 TTACCGACTGTACGCCAAGAAGGGCAGGGACGACAAACCCTGGTTTGATGGCCTAGACAG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11561R-1.in silico 1 TTACCGACTGTACGCCAAGAAGGGCAGGGACGACAAACCCTGGTTTGATGGCCTAGACAG 60
11561R-1.IR_full 61 CAGGCACATCCAGTGTGTCCGACGTGCCCGTTGCTACCCCACCTCGAACGCAACCAACAC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11561R-1.in silico 61 CAGGCACATCCAGTGTGTCCGACGTGCCCGTTGCTACCCCACCTCGAACGCAACCAACAC 120
11561R-1.IR_full 121 CTGTTTCGGCTCAAAATTG<span class="snp"><tt>G</tt></span>CCTATGAGCTGAGCAGCCTAGATCTCACCGACTTCCACAC 180
|||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||||
11561R-1.in silico 121 CTGTTTCGGCTCAAAATTG<span class="snp"><tt>C</tt></span>CCTATGAGCTGAGCAGCCTAGATCTCACCGACTTCCACAC 180
11561R-1.IR_full 181 CGAAAAGGAGCTGAACGATAAGCTGAACGACTACTATGCCCTGAAGCACGTGCCCAAATG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11561R-1.in silico 181 CGAAAAGGAGCTGAACGATAAGCTGAACGACTACTATGCCCTGAAGCACGTGCCCAAATG 240
11561R-1.IR_full 241 TTGGGCAGCTATACAGCCCTTTTTGTGCGCCGTCTTTAAGCCGAAGTGTGAAAAAATCAA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11561R-1.in silico 241 TTGGGCAGCTATACAGCCCTTTTTGTGCGCCGTCTTTAAGCCGAAGTGTGAAAAAATCAA 300
11561R-1.IR_full 301 CGGCGAGGACATGGTCTACCTGCCATCTTACGAGATGTGCCGAATTACCATGGAACCCTG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11561R-1.in silico 301 CGGCGAGGACATGGTCTACCTGCCATCTTACGAGATGTGCCGAATTACCATGGAACCCTG 360
11561R-1.IR_full 361 TCGCATTTTGTACAACACGACGTTTTTCCCAAAATTCCTTCGCTGCAACGAAACACTCTT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11561R-1.in silico 361 TCGCATTTTGTACAACACGACGTTTTTCCCAAAATTCCTTCGCTGCAACGAAACACTCTT 420
11561R-1.IR_full 421 TCCGACGAAATGCACAAACGGAGCACGAGGAATGAAATTCAACGGAACTGGCCAGTGTTT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11561R-1.in silico 421 TCCGACGAAATGCACAAACGGAGCACGAGGAATGAAATTCAACGGAACTGGCCAGTGTTT 480
11561R-1.IR_full 481 AAGTCCTTTGGTTCCGACAG 500
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11561R-1.in silico 481 AAGTCCTTTGGTTCCGACAG 500
</PRE>
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_078719.2 |
CG11561-RA (smo), mRNA |
| 0 |
0 |
4 |
2 |
5 |
NM_137046.3 |
CG6191-RA (CG6191), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_206188.1 |
CG30296-RA, transcript variant A (CG30296), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_166418.1 |
CG30296-RD, transcript variant D (CG30296), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_137693.1 |
CG30296-RC, transcript variant C (CG30296), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_140687.4 |
CG6664-RA, transcript variant A (CG6664), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_168710.3 |
CG6664-RC, transcript variant C (CG6664), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_168709.3 |
CG6664-RB, transcript variant B (CG6664), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_168711.2 |
CG6664-RD, transcript variant D (CG6664), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_057659.2 |
CG9652-RA (DopR), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_140963.1 |
CG5847-RA (CG5847), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_165132.1 |
CG18109-RA (CG18109), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_136013.1 |
CG15148-RA, transcript variant A (btv), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_001042905.1 |
CG15148-RC, transcript variant C (btv), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_001042904.1 |
CG15148-RB, transcript variant B (btv), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_165233.1 |
CG5674-RC, transcript variant C (CG5674), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_080051.2 |
CG1214-RA (ru), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_001043185.1 |
CG12460-RA (CG12460), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_167505.1 |
CG4211-RB, transcript variant B (nonA), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_001014747.1 |
CG4211-RC, transcript variant C (nonA), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_078643.2 |
CG4211-RA, transcript variant A (nonA), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_166742.1 |
CG31998-RA (CG31998), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_001014750.1 |
CG7826-RD, transcript variant D (mnb), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_170658.2 |
CG7826-RC, transcript variant C (mnb), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_167581.2 |
CG7826-RA, transcript variant A (mnb), mRNA |
| 0 |
0 |
0 |
6 |
7 |
NM_164985.1 |
CG6756-RB, transcript variant B (Tom70), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_167081.2 |
CG4094-RB, transcript variant B (l(1)G0255), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_132111.2 |
CG4094-RA, transcript variant A (l(1)G0255), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_079372.2 |
CG6215-RA (CkIIalpha-i1), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_079123.2 |
CG4556-RA (eyc), mRNA |
|
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Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
dme04341
:
Hedgehog signaling pathway - fly
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