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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

11534R-1

Symbol

CG11534

Full Name

CG11534

CG No

CG11534

Old CG No

CG11534

Synonyms

BEST:GH12489, CG11534

Accession No (Link to NCBI)

NM_140292.2

Inserted Chr.

lll

Insertional Mutation

lethal

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Du J, Zhang J, Su Y, Liu M, Ospina JK, Yang S, Zhu AJ.<br> In vivo RNAi screen reveals neddylation genes as novel regulators of Hedgehog signaling.<br> PLoS One (2011) 6(9) e24168 [ PubMed ID = 21931660 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATGAGCTCCTGGAAGGACAGT

Primer Seq. 3'

aatctagaggtaccTTCCGTTTGCCATGATGCGGG

Predicted Fragment Size

IR fragment full Seq

0001 ATGAGCTCCT GGAAGGACAG TCTCACGAGC ATTCCGGGCA CGGTGGCGCA GTTGATTAAC 
0061 GAGAGCGCCA GCAACCTGTT ACACGCCTCA TCCACGCTGG GATCAACAGT TGGCCTTGGA 
0121 GGCTCTGGCT CCACTGGATC TGGATCCGAG GCCGGAGGCT CCGAGGNAAA GTGGTCCACA 
0181 AGGTGCGGAG TANCAGGGCG TTGCCCATTC CCGCGTCCTT GGTGCGCGAG CAATGGCGCC 
0241 TAATCTTCAC CAGCGATGCC AATATCCAGG ATTTGCAGGC GGCCATCGCC CATTGCAGAG 
0301 ATCTCGTCCT GCTGAGCGAG GAACTCAGCG AGGAGCGTCG CTGGNTTGGT GCGGCACCTG 
0361 GTAGATTTGC GATACTCACT GCAGGAGCTG GAGGAGGCCC AGGAGCAGCA TTCCCTATCG 
0421 TCGGACATGG TAGTTATGAA TGCCATACGT GCTGTGGTGG GTCATCATTT TGTGCCCCAT 
0481 CACCCGCATC ATGGCAAACG GAA

in silico PCR Fragment

0001 ATGAGCTCCT GGAAGGACAG TCTCACGAGC ATTCCGGGCA CGGTGGCGCA GTTGATTAAC 
0061 GAGAGCGCCA GCAACCTGTT ACACGCCTCA TCCACGCTGG GATCAACAGT TGGCCTTGGA 
0121 GGCTCTGGCT CCACTGGATC TGGATCCGAG GCCGGAGGCT CCGAGG-AAA GTGGTCCACA 
0181 AGGTGCGGAG TA-CAGGGCG TTGCCCATTC CCGCGTCCTT GGTGCGCGAG CAATGGCGCC 
0241 TAATCTTCAC CAGCGATGCC AATATCCAGG ATTTGCAGGC GGCCATCGCC CATTGCAGAG 
0301 ATCTCGTCCT GCTGAGCGAG GAACTCAGCG AGGAGCGTCG CTGG-TTGGT GCGGCACCTG 
0361 GTAGATTTGC GATACTCACT GCAGGAGCTG GAGGAGGCCC AGGAGCAGCA TTCCCTATCG 
0421 TCGGACATGG TAGTTATGAA TGCCATACGT GCTGTGGTGG GTCATCATTT TGTGCCCCAT 
0481 CACCCGCATC ATGGCAAACG GAA

Assemble Data

<PRE>

11534R-1.IR_full       1   ATGAGCTCCTGGAAGGACAGTCTCACGAGCATTCCGGGCACGGTGGCGCAGTTGATTAAC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11534R-1.in silico     1   ATGAGCTCCTGGAAGGACAGTCTCACGAGCATTCCGGGCACGGTGGCGCAGTTGATTAAC 60


11534R-1.IR_full       61  GAGAGCGCCAGCAACCTGTTACACGCCTCATCCACGCTGGGATCAACAGTTGGCCTTGGA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11534R-1.in silico     61  GAGAGCGCCAGCAACCTGTTACACGCCTCATCCACGCTGGGATCAACAGTTGGCCTTGGA 120


11534R-1.IR_full       121 GGCTCTGGCTCCACTGGATCTGGATCCGAGGCCGGAGGCTCCGAGG<span class="snp"><tt>N</tt></span>AAAGTGGTCCACA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||
11534R-1.in silico     121 GGCTCTGGCTCCACTGGATCTGGATCCGAGGCCGGAGGCTCCGAGG<span class="snp"><tt>-</tt></span>AAAGTGGTCCACA 180


11534R-1.IR_full       181 AGGTGCGGAGTA<span class="snp"><tt>N</tt></span>CAGGGCGTTGCCCATTCCCGCGTCCTTGGTGCGCGAGCAATGGCGCC 240
                           ||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||
11534R-1.in silico     181 AGGTGCGGAGTA<span class="snp"><tt>-</tt></span>CAGGGCGTTGCCCATTCCCGCGTCCTTGGTGCGCGAGCAATGGCGCC 240


11534R-1.IR_full       241 TAATCTTCACCAGCGATGCCAATATCCAGGATTTGCAGGCGGCCATCGCCCATTGCAGAG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11534R-1.in silico     241 TAATCTTCACCAGCGATGCCAATATCCAGGATTTGCAGGCGGCCATCGCCCATTGCAGAG 300


11534R-1.IR_full       301 ATCTCGTCCTGCTGAGCGAGGAACTCAGCGAGGAGCGTCGCTGG<span class="snp"><tt>N</tt></span>TTGGTGCGGCACCTG 360
                           ||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||
11534R-1.in silico     301 ATCTCGTCCTGCTGAGCGAGGAACTCAGCGAGGAGCGTCGCTGG<span class="snp"><tt>-</tt></span>TTGGTGCGGCACCTG 360


11534R-1.IR_full       361 GTAGATTTGCGATACTCACTGCAGGAGCTGGAGGAGGCCCAGGAGCAGCATTCCCTATCG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11534R-1.in silico     361 GTAGATTTGCGATACTCACTGCAGGAGCTGGAGGAGGCCCAGGAGCAGCATTCCCTATCG 420


11534R-1.IR_full       421 TCGGACATGGTAGTTATGAATGCCATACGTGCTGTGGTGGGTCATCATTTTGTGCCCCAT 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11534R-1.in silico     421 TCGGACATGGTAGTTATGAATGCCATACGTGCTGTGGTGGGTCATCATTTTGTGCCCCAT 480


11534R-1.IR_full       481 CACCCGCATCATGGCAAACGGAA 503
                           |||||||||||||||||||||||
11534R-1.in silico     481 CACCCGCATCATGGCAAACGGAA 503

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_140292.2	CG11534-RA (CG11534), mRNA
0	0	3	3	3	NM_205898.1	CG33281-RA (CG33281), mRNA
0	0	2	4	7	NM_165838.2	CG17835-RC, transcript variant C (inv), mRNA
0	0	2	4	7	NM_078975.3	CG17835-RB, transcript variant B (inv), mRNA
0	0	2	4	7	NM_165837.2	CG17835-RA, transcript variant A (inv), mRNA
0	0	2	4	7	NM_165839.2	CG17835-RD, transcript variant D (inv), mRNA
0	0	2	2	4	NM_079558.4	CG31453-RA (CG31453), mRNA
0	0	1	13	17	NM_165182.1	CG17927-RG, transcript variant G (Mhc), mRNA
0	0	1	13	17	NM_165183.1	CG17927-RE, transcript variant E (Mhc), mRNA
0	0	1	13	17	NM_165184.1	CG17927-RJ, transcript variant J (Mhc), mRNA
0	0	1	13	17	NM_165185.1	CG17927-RF, transcript variant F (Mhc), mRNA
0	0	1	13	17	NM_165186.1	CG17927-RD, transcript variant D (Mhc), mRNA
0	0	1	13	17	NM_165187.1	CG17927-RA, transcript variant A (Mhc), mRNA
0	0	1	13	17	NM_165188.1	CG17927-RI, transcript variant I (Mhc), mRNA
0	0	1	13	17	NM_078863.4	CG17927-RH, transcript variant H (Mhc), mRNA
0	0	1	13	17	NM_165190.1	CG17927-RK, transcript variant K (Mhc), mRNA
0	0	1	13	17	NM_165191.1	CG17927-RL, transcript variant L (Mhc), mRNA
0	0	1	13	17	NM_165192.1	CG17927-RM, transcript variant M (Mhc), mRNA
0	0	1	13	17	NM_165189.1	CG17927-RB, transcript variant B (Mhc), mRNA
0	0	1	13	17	NM_165181.1	CG17927-RC, transcript variant C (Mhc), mRNA
0	0	1	4	8	NM_079008.2	CG6315-RA, transcript variant A (fl(2)d), mRNA
0	0	1	4	8	NM_166010.1	CG6315-RB, transcript variant B (fl(2)d), mRNA
0	0	1	4	8	NM_135178.2	CG9507-RA (CG9507), mRNA
0	0	1	4	6	NM_165978.1	CG17041-RC, transcript variant C (CG17041), mRNA
0	0	1	4	6	NM_165977.1	CG17041-RB, transcript variant B (CG17041), mRNA
0	0	1	4	6	NM_137016.3	CG17041-RA, transcript variant A (CG17041), mRNA
0	0	1	3	3	NM_139590.2	CG1134-RA (CG1134), mRNA
0	0	1	3	2	NM_144220.1	CG12714-RB (CG12714), mRNA
0	0	1	2	7	NM_078787.2	CG13391-RA, transcript variant A (Aats-ala), mRNA
0	0	1	2	7	NM_205934.1	CG13391-RB, transcript variant B (Aats-ala), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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