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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
11440R-2 |
| Symbol |
laza |
Full Name |
lazaro |
| CG No |
CG11440 |
Old CG No |
CG11440 |
| Synonyms |
WUN-like, laza, Laza, CG11440 |
| Accession No (Link to NCBI) |
NM_141134.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
pupal lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.<br> Zyxin links fat signaling to the hippo pathway.<br> PLoS Biol (2011) 9(6) e1000624
[
PubMed ID = 21666802
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCCTACAAGGACTGCACGATCA |
| Primer Seq. 3' |
aatctagaggtaccGGCAGCCATAAGGAGCAGGAA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CCTACAAGGA CTGCACGATC ACGGTGCCGA TGCTACTGCT CATGATGCTC CTGCTCCCGA
0061 TGCTCTTCGT GGCCGTGGTG GAGATCATGC GGATCTGCAA GCGCTTCCGc ACCAGGCTCT
0121 ACTTCCGCAA TCTGTGGCGC GCAGAGGCCA CCTTCAGCTT CGGCTTCATA GCCACCTATC
0181 TCACCACCGA GCTGGCGAAg cNCGCCGTCg GGNGGNTGAg GCCGCACTTC TTCCACGGTT
0241 GCCAGCCGCG CCTCGACGAT GGCAGCTCCT GCTCGGACCT CCAGAACGCT GAGCTGTACG
0301 TGGAGCAGTT CCACTGCACT AACAACAACC TCTCCACGCG ACAGATCCGC GAGTTGCACG
0361 TCAGTTTCCC GAGTGCCCAC AGCAGCTTGA GCTTCTATTC GATGGTCCTG CTGGCCCTCT
0421 ACGTACACGG GGTGTGGCGG GGACGAGGGG GAGTGCGAGT CCTGCGGCAC GTCCTGCAGT
0481 TCCTGCTCCT TATGGCTGCC |
| in silico PCR Fragment |
0001 CCTACAAGGA CTGCACGATC ACGGTGCCGA TGCTACTGCT CATGATGCTC CTGCTCCCGA
0061 TGCTCTTCGT GGCCGTGGTG GAGATCATGC GGATCTGCAA GCGCTTCCGC ACCAGGCTCT
0121 ACTTCCGCAA TCTGTGGCGC GCAGAGGCCA CCTTCAGCTT CGGCTTCATA GCCACCTATC
0181 TCACCACCGA GCTGGCGAAG CACGCCGTCG GGCGGCTGAG GCCGCACTTC TTCCACGGTT
0241 GCCAGCCGCG CCTCGACGAT GGCAGCTCCT GCTCGGACCT CCAGAACGCT GAGCTGTACG
0301 TGGAGCAGTT CCACTGCACT AACAACAACC TCTCCACGCG ACAGATCCGC GAGTTGCACG
0361 TCAGTTTCCC GAGTGCCCAC AGCAGCTTGA GCTTCTATTC GATGGTCCTG CTGGCCCTCT
0421 ACGTACACGG GGTGTGGCGG GGACGAGGGG GAGTGCGAGT CCTGCGGCAC GTCCTGCAGT
0481 TCCTGCTCCT TATGGCTGCC |
| Assemble Data |
<PRE>
11440R-2.IR_full 1 CCTACAAGGACTGCACGATCACGGTGCCGATGCTACTGCTCATGATGCTCCTGCTCCCGA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11440R-2.in silico 1 CCTACAAGGACTGCACGATCACGGTGCCGATGCTACTGCTCATGATGCTCCTGCTCCCGA 60
11440R-2.IR_full 61 TGCTCTTCGTGGCCGTGGTGGAGATCATGCGGATCTGCAAGCGCTTCCGCACCAGGCTCT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11440R-2.in silico 61 TGCTCTTCGTGGCCGTGGTGGAGATCATGCGGATCTGCAAGCGCTTCCGCACCAGGCTCT 120
11440R-2.IR_full 121 ACTTCCGCAATCTGTGGCGCGCAGAGGCCACCTTCAGCTTCGGCTTCATAGCCACCTATC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11440R-2.in silico 121 ACTTCCGCAATCTGTGGCGCGCAGAGGCCACCTTCAGCTTCGGCTTCATAGCCACCTATC 180
11440R-2.IR_full 181 TCACCACCGAGCTGGCGAAGC<span class="snp"><tt>N</tt></span>CGCCGTCGGG<span class="snp"><tt>N</tt></span>GG<span class="snp"><tt>N</tt></span>TGAGGCCGCACTTCTTCCACGGTT 240
|||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||<span class="snp"><tt> </tt></span>||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||
11440R-2.in silico 181 TCACCACCGAGCTGGCGAAGC<span class="snp"><tt>A</tt></span>CGCCGTCGGG<span class="snp"><tt>C</tt></span>GG<span class="snp"><tt>C</tt></span>TGAGGCCGCACTTCTTCCACGGTT 240
11440R-2.IR_full 241 GCCAGCCGCGCCTCGACGATGGCAGCTCCTGCTCGGACCTCCAGAACGCTGAGCTGTACG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11440R-2.in silico 241 GCCAGCCGCGCCTCGACGATGGCAGCTCCTGCTCGGACCTCCAGAACGCTGAGCTGTACG 300
11440R-2.IR_full 301 TGGAGCAGTTCCACTGCACTAACAACAACCTCTCCACGCGACAGATCCGCGAGTTGCACG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11440R-2.in silico 301 TGGAGCAGTTCCACTGCACTAACAACAACCTCTCCACGCGACAGATCCGCGAGTTGCACG 360
11440R-2.IR_full 361 TCAGTTTCCCGAGTGCCCACAGCAGCTTGAGCTTCTATTCGATGGTCCTGCTGGCCCTCT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11440R-2.in silico 361 TCAGTTTCCCGAGTGCCCACAGCAGCTTGAGCTTCTATTCGATGGTCCTGCTGGCCCTCT 420
11440R-2.IR_full 421 ACGTACACGGGGTGTGGCGGGGACGAGGGGGAGTGCGAGTCCTGCGGCACGTCCTGCAGT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11440R-2.in silico 421 ACGTACACGGGGTGTGGCGGGGACGAGGGGGAGTGCGAGTCCTGCGGCACGTCCTGCAGT 480
11440R-2.IR_full 481 TCCTGCTCCTTATGGCTGCC 500
||||||||||||||||||||
11440R-2.in silico 481 TCCTGCTCCTTATGGCTGCC 500
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
8 |
NM_141134.1 |
CG11440-RA (CG11440), mRNA |
| 0.41 |
2 |
2 |
3 |
30 |
NM_142710.2 |
CG16791-RA (CG16791), mRNA |
| 0 |
0 |
4 |
2 |
5 |
NM_170038.1 |
CG31151-RB, transcript variant B (wge), mRNA |
| 0 |
0 |
4 |
2 |
5 |
NM_170037.1 |
CG31151-RA, transcript variant A (wge), mRNA |
| 0 |
0 |
4 |
2 |
5 |
NM_001043281.1 |
CG31151-RC, transcript variant C (wge), mRNA |
| 0 |
0 |
4 |
2 |
4 |
NM_130656.2 |
CG9904-RA (CG9904), mRNA |
| 0 |
0 |
4 |
2 |
4 |
NM_080025.1 |
CG11387-RA, transcript variant A (ct), mRNA |
| 0 |
0 |
4 |
2 |
3 |
NM_140372.1 |
CG14115-RA (CG14115), mRNA |
| 0 |
0 |
3 |
3 |
4 |
NM_078710.2 |
CG1666-RA (Hlc), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_164790.1 |
CG31605-RG, transcript variant G (Bsg), mRNA |
| 0 |
0 |
2 |
5 |
3 |
NM_164618.1 |
CG14041-RA, transcript variant A (SP555), mRNA |
| 0 |
0 |
2 |
5 |
3 |
NM_144359.2 |
CG14041-RB, transcript variant B (SP555), mRNA |
| 0 |
0 |
2 |
5 |
3 |
NM_001042871.1 |
CG14042-RA, transcript variant A (CG14042), mRNA |
| 0 |
0 |
2 |
5 |
3 |
NM_001042872.1 |
CG14042-RB, transcript variant B (CG14042), mRNA |
| 0 |
0 |
2 |
4 |
9 |
NM_136158.2 |
CG10538-RA (CdGAPr), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_164696.1 |
CG31635-RA (CG31635), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_001032402.1 |
CG33957-RB, transcript variant B (cp309), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_001032401.1 |
CG33957-RC, transcript variant C (cp309), mRNA |
| 0 |
0 |
1 |
8 |
22 |
NM_143310.2 |
CG12885-RA (CG12885), mRNA |
| 0 |
0 |
1 |
7 |
5 |
NM_132704.1 |
CG11071-RA (CG11071), mRNA |
| 0 |
0 |
1 |
5 |
14 |
NM_080026.2 |
CG10847-RB, transcript variant B (enc), mRNA |
| 0 |
0 |
1 |
5 |
14 |
NM_206270.1 |
CG10847-RA, transcript variant A (enc), mRNA |
| 0 |
0 |
1 |
5 |
6 |
NM_168254.1 |
CG7915-RB, transcript variant B (Ect4), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_001043129.1 |
CG7915-RC, transcript variant C (Ect4), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_168542.1 |
CG10171-RB, transcript variant B (CG10171), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_140382.1 |
CG10171-RA, transcript variant A (CG10171), mRNA |
| 0 |
0 |
1 |
4 |
9 |
NM_140893.2 |
CG15881-RB, transcript variant B (CG15881), mRNA |
| 0 |
0 |
1 |
4 |
9 |
NM_001043151.1 |
CG34116-RA (CG34116), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_165508.1 |
CG11084-RA, transcript variant A (pk), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_143348.2 |
CG4849-RA (CG4849), mRNA |
|
| Wing Disc |
GFP
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Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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JNK signal
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Caspase-3 signal
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Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
dme00561
:
Glycerolipid metabolism
dme00564
:
Glycerophospholipid metabolism
dme00565
:
Ether lipid metabolism
dme00600
:
Sphingolipid metabolism
dme01100
:
Metabolic pathways
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