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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

11401R-3

Symbol

Trxr-2

Full Name

thioredoxin reductase 2

CG No

CG11401

Old CG No

CG11401

Synonyms

trxr-2, Trxr2, DmTrxR-2, CG11401, Trxr-2

Accession No (Link to NCBI)

NM_079492.2

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGAATGTGGGCTGCATCCCCAA

Primer Seq. 3'

aatctagaggtaccGCACAATGGAGCGCACCATGA

Predicted Fragment Size

IR fragment full Seq

0001 GAATGTGGGC TGCATCCCCA AGAAGCTAAT GCACCAGGCC TCGCTGCTGG GCGAGGCTGT 
0061 TCACGAGGCG GTGGCCTACG GCTGGAATGT AGACGACACG AACATACGGC CCGATTGGCG 
0121 CAAGTTGGTG CGCTCCGTCC AGAACCACAT CAAGTCCGTC AACTGGGTGA CCCGCGTGGA 
0181 CCTGCGCGAC AAAAAGGTGG AGTACGTAAA TTCGATGGCC ACCTTTCGCG ACAGCCACAC 
0241 CATCGAGTAT GTGGCGATGC NAGGTGCCGA GCACCGTCAA GTGACCTCAG AGTACGTGGT 
0301 GGTAGCCGTC GGCGGGAGGC CACGCTACCC GGACATTCCC GGAGCCGTTG AACTGGGCAT 
0361 CACCAGCGAC GATATATTCA GCTACGAGCG AGAGCCGGGT CGTACCCTTG TGGTGGGCGC 
0421 CGGATACGTG GGTCTCGAGT GCGCCTGTTT CCTTAAGGGT CTCGGCTACG AGCCCACTGT 
0481 CATGGTGCGC TCCATTGTGC

in silico PCR Fragment

0001 GAATGTGGGC TGCATCCCCA AGAAGCTAAT GCACCAGGCC TCGCTGCTGG GCGAGGCTGT 
0061 TCACGAGGCG GTGGCCTACG GCTGGAATGT AGACGACACG AACATACGGC CCGATTGGCG 
0121 CAAGTTGGTG CGCTCCGTCC AGAACCACAT CAAGTCCGTC AACTGGGTGA CCCGCGTGGA 
0181 CCTGCGCGAC AAAAAGGTGG AGTACGTAAA TTCGATGGCC ACCTTTCGCG ACAGCCACAC 
0241 CATCGAGTAT GTGGCGATGC CAGGTGCCGA GCACCGTCAA GTGACCTCAG AGTACGTGGT 
0301 GGTAGCCGTC GGCGGGAGGC CACGCTACCC GGACATTCCC GGAGCCGTTG AACTGGGCAT 
0361 CACCAGCGAC GATATATTCA GCTACGAGCG AGAGCCGGGT CGTACCCTTG TGGTGGGCGC 
0421 CGGATACGTG GGTCTCGAGT GCGCCTGTTT CCTTAAGGGT CTCGGCTACG AGCCCACTGT 
0481 CATGGTGCGC TCCATTGTGC

Assemble Data

<PRE>

11401R-3.IR_full       1   GAATGTGGGCTGCATCCCCAAGAAGCTAATGCACCAGGCCTCGCTGCTGGGCGAGGCTGT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11401R-3.in silico     1   GAATGTGGGCTGCATCCCCAAGAAGCTAATGCACCAGGCCTCGCTGCTGGGCGAGGCTGT 60


11401R-3.IR_full       61  TCACGAGGCGGTGGCCTACGGCTGGAATGTAGACGACACGAACATACGGCCCGATTGGCG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11401R-3.in silico     61  TCACGAGGCGGTGGCCTACGGCTGGAATGTAGACGACACGAACATACGGCCCGATTGGCG 120


11401R-3.IR_full       121 CAAGTTGGTGCGCTCCGTCCAGAACCACATCAAGTCCGTCAACTGGGTGACCCGCGTGGA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11401R-3.in silico     121 CAAGTTGGTGCGCTCCGTCCAGAACCACATCAAGTCCGTCAACTGGGTGACCCGCGTGGA 180


11401R-3.IR_full       181 CCTGCGCGACAAAAAGGTGGAGTACGTAAATTCGATGGCCACCTTTCGCGACAGCCACAC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11401R-3.in silico     181 CCTGCGCGACAAAAAGGTGGAGTACGTAAATTCGATGGCCACCTTTCGCGACAGCCACAC 240


11401R-3.IR_full       241 CATCGAGTATGTGGCGATGC<span class="snp"><tt>N</tt></span>AGGTGCCGAGCACCGTCAAGTGACCTCAGAGTACGTGGT 300
                           ||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||
11401R-3.in silico     241 CATCGAGTATGTGGCGATGC<span class="snp"><tt>C</tt></span>AGGTGCCGAGCACCGTCAAGTGACCTCAGAGTACGTGGT 300


11401R-3.IR_full       301 GGTAGCCGTCGGCGGGAGGCCACGCTACCCGGACATTCCCGGAGCCGTTGAACTGGGCAT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11401R-3.in silico     301 GGTAGCCGTCGGCGGGAGGCCACGCTACCCGGACATTCCCGGAGCCGTTGAACTGGGCAT 360


11401R-3.IR_full       361 CACCAGCGACGATATATTCAGCTACGAGCGAGAGCCGGGTCGTACCCTTGTGGTGGGCGC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11401R-3.in silico     361 CACCAGCGACGATATATTCAGCTACGAGCGAGAGCCGGGTCGTACCCTTGTGGTGGGCGC 420


11401R-3.IR_full       421 CGGATACGTGGGTCTCGAGTGCGCCTGTTTCCTTAAGGGTCTCGGCTACGAGCCCACTGT 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11401R-3.in silico     421 CGGATACGTGGGTCTCGAGTGCGCCTGTTTCCTTAAGGGTCTCGGCTACGAGCCCACTGT 480


11401R-3.IR_full       481 CATGGTGCGCTCCATTGTGC 500
                           ||||||||||||||||||||
11401R-3.in silico     481 CATGGTGCGCTCCATTGTGC 500

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_079492.2	CG11401-RA (Trxr-2), mRNA
5.18	25	40	77	54	NM_078527.2	CG2151-RA, transcript variant A (Trxr-1), mRNA
5.18	25	40	77	54	NM_167149.1	CG2151-RB, transcript variant B (Trxr-1), mRNA
5.18	25	40	77	54	NM_167150.1	CG2151-RC, transcript variant C (Trxr-1), mRNA
3.52	17	25	60	24	NM_167151.1	CG32715-RA (CG32715), mRNA
0.2	1	2	2	2	NM_079750.3	CG5436-RA (Hsp68), mRNA
0	0	2	3	4	NM_132640.2	CG1770-RA, transcript variant A (HDAC4), mRNA
0	0	2	3	4	NM_001014736.1	CG1770-RC, transcript variant C (HDAC4), mRNA
0	0	2	3	4	NM_167356.1	CG1770-RB, transcript variant B (HDAC4), mRNA
0	0	2	2	2	NM_168194.1	CG32389-RA (CG32389), mRNA
0	0	1	6	8	NM_167654.1	CG3400-RF, transcript variant F (Pfrx), mRNA
0	0	1	6	8	NM_167655.1	CG3400-RH, transcript variant H (Pfrx), mRNA
0	0	1	6	8	NM_167653.1	CG3400-RE, transcript variant E (Pfrx), mRNA
0	0	1	6	8	NM_167651.1	CG3400-RA, transcript variant A (Pfrx), mRNA
0	0	1	6	8	NM_167656.1	CG3400-RI, transcript variant I (Pfrx), mRNA
0	0	1	6	8	NM_058104.4	CG3400-RB, transcript variant B (Pfrx), mRNA
0	0	1	6	8	NM_058103.2	CG3400-RG, transcript variant G (Pfrx), mRNA
0	0	1	6	8	NM_167652.1	CG3400-RD, transcript variant D (Pfrx), mRNA
0	0	1	4	3	NM_142465.1	CG18617-RB, transcript variant B (Vha100-2), mRNA
0	0	1	4	3	NM_169810.1	CG18617-RA, transcript variant A (Vha100-2), mRNA
0	0	1	3	8	NM_132420.2	CG32676-RA (CG32676), mRNA
0	0	1	3	2	NM_140197.1	CG18490-RB, transcript variant B (CG18490), mRNA
0	0	1	3	2	NM_168453.1	CG18490-RC, transcript variant C (CG18490), mRNA
0	0	1	3	2	NM_079092.2	CG2956-RA, transcript variant A (twi), mRNA
0	0	1	3	2	NM_001038878.1	CG2956-RB, transcript variant B (twi), mRNA
0	0	1	2	10	NM_143024.2	CG13630-RA (CG13630), mRNA
0	0	1	2	6	NM_001014681.1	CG33553-RD, transcript variant D (Doa), mRNA
0	0	1	2	6	NM_001014678.1	CG33553-RE, transcript variant E (Doa), mRNA
0	0	1	2	5	NM_001014680.1	CG33553-RA, transcript variant A (Doa), mRNA
0	0	1	2	5	NM_001043300.1	CG33553-RF, transcript variant F (Doa), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

dme00450 : Selenocompound metabolism

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