NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

11327R-1

Symbol

CG11327

Full Name

CG11327

CG No

CG11327

Old CG No

CG11327

Synonyms

CG11327

Accession No (Link to NCBI)

NM_135221.2

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATCCCAGGAAGAGCTGAAGCG

Primer Seq. 3'

aatctagaggtaccCCTGGTCAATATCATCGGGCA

Predicted Fragment Size

IR fragment full Seq

0001 ATCCCAGGAA GAGCTGAAGC GGACTGAGAA CAACAAGAAT GTGAAAAACT CCGAAGAATG 
0061 TCAAGCAATG GAGGATGATG TGGACGCGGT TGTCCCATCT TCGTCGGAGA GTGAGCTGCG 
0121 GCCTCGCAGT TCGGGAGTTA GGCCCACTAA AATTCCGGCT CCACTTTTCT TGGGACCCAG 
0181 TGCCAGCAGC CGGATCTACA GTTCACTGAT GCCCGAGAGA AAGCGACGCT CCTCCATGGA 
0241 AAGTCAACAG ATGGCCGACG ATTCTGGCAT CAATTTGGAT TGCTCCACGG ACGAGCAGCA 
0301 GACTACGCTG TCGGCCAGAC CGAGTCCAAT GCTGCAGCCG ATGCTCGACA GGATTCCGGA 
0361 CACTAGCAGC GAGTTGGAGC TCCGACTGGC AGAGGAGCAG ACTCTCAAGG AACTTCAACG 
0421 CGTCGTCGCC GAAATGGAGG CTGATGTGGC TTTTAATAAT TTAGATATGC AAAATCTACT 
0481 GCCCGATGAT ATTGACCAGG

in silico PCR Fragment

0001 ATCCCAGGAA GAGCTGAAGC GGACTGAGAA CAACAAGAAT GTGAAAAACT CCGAAGAATG 
0061 TCAATCAATG GAAAACGATG TGGACGCGGT TGTCCCATCT TCGTCGGAGA GTGAGCTGCG 
0121 GCCTCGCAGT TCGGGAGTTA GGCCCACTAA AATTCCGGCT CCACTTTTCT TGGGACCCAG 
0181 TGCCAGCAGC CGGATCTACA GTTCACTGAT GCCCGAGAGA AAGCGACGCT CCTCCATGGA 
0241 AAGTCAACAG ATGGCCGACG ATTCTGGCAT CAATTTGGAT TGCTCCACGG ACGAGCAGCA 
0301 GACTACGCTG TCGGCCAGAC CGAGTCCAAT GCTGCAGCCG ATGCTCGACA GGATTCCGGA 
0361 CACTAGCAGC GAGTTGGAGC TCCGACTGGC AGAGGAGCAG ACTCTCAAGG AACTTCAACG 
0421 CGTCGTCGCC GAAATGGAGG CTGATGTGGC TTTTAATAAT TTAGATATGC AAAATCTACT 
0481 GCCCGATGAT ATTGACCAGG

Assemble Data

<PRE>

11327R-1.IR_full       1   ATCCCAGGAAGAGCTGAAGCGGACTGAGAACAACAAGAATGTGAAAAACTCCGAAGAATG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11327R-1.in silico     1   ATCCCAGGAAGAGCTGAAGCGGACTGAGAACAACAAGAATGTGAAAAACTCCGAAGAATG 60


11327R-1.IR_full       61  TCAA<span class="snp"><tt>G</tt></span>CAATGGA<span class="snp"><tt>G</tt></span><span class="snp"><tt>G</tt></span>A<span class="snp"><tt>T</tt></span>GATGTGGACGCGGTTGTCCCATCTTCGTCGGAGAGTGAGCTGCG 120
                           ||||<span class="snp"><tt>&nbsp;</tt></span>|||||||<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>|<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||
11327R-1.in silico     61  TCAA<span class="snp"><tt>T</tt></span>CAATGGA<span class="snp"><tt>A</tt></span><span class="snp"><tt>A</tt></span>A<span class="snp"><tt>C</tt></span>GATGTGGACGCGGTTGTCCCATCTTCGTCGGAGAGTGAGCTGCG 120


11327R-1.IR_full       121 GCCTCGCAGTTCGGGAGTTAGGCCCACTAAAATTCCGGCTCCACTTTTCTTGGGACCCAG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11327R-1.in silico     121 GCCTCGCAGTTCGGGAGTTAGGCCCACTAAAATTCCGGCTCCACTTTTCTTGGGACCCAG 180


11327R-1.IR_full       181 TGCCAGCAGCCGGATCTACAGTTCACTGATGCCCGAGAGAAAGCGACGCTCCTCCATGGA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11327R-1.in silico     181 TGCCAGCAGCCGGATCTACAGTTCACTGATGCCCGAGAGAAAGCGACGCTCCTCCATGGA 240


11327R-1.IR_full       241 AAGTCAACAGATGGCCGACGATTCTGGCATCAATTTGGATTGCTCCACGGACGAGCAGCA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11327R-1.in silico     241 AAGTCAACAGATGGCCGACGATTCTGGCATCAATTTGGATTGCTCCACGGACGAGCAGCA 300


11327R-1.IR_full       301 GACTACGCTGTCGGCCAGACCGAGTCCAATGCTGCAGCCGATGCTCGACAGGATTCCGGA 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11327R-1.in silico     301 GACTACGCTGTCGGCCAGACCGAGTCCAATGCTGCAGCCGATGCTCGACAGGATTCCGGA 360


11327R-1.IR_full       361 CACTAGCAGCGAGTTGGAGCTCCGACTGGCAGAGGAGCAGACTCTCAAGGAACTTCAACG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11327R-1.in silico     361 CACTAGCAGCGAGTTGGAGCTCCGACTGGCAGAGGAGCAGACTCTCAAGGAACTTCAACG 420


11327R-1.IR_full       421 CGTCGTCGCCGAAATGGAGGCTGATGTGGCTTTTAATAATTTAGATATGCAAAATCTACT 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11327R-1.in silico     421 CGTCGTCGCCGAAATGGAGGCTGATGTGGCTTTTAATAATTTAGATATGCAAAATCTACT 480


11327R-1.IR_full       481 GCCCGATGATATTGACCAGG 500
                           ||||||||||||||||||||
11327R-1.in silico     481 GCCCGATGATATTGACCAGG 500

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	2	NM_135221.2	CG11327-RA, transcript variant A (CG11327), mRNA
100	482	0	0	2	NM_175979.1	CG11327-RB, transcript variant B (CG11327), mRNA
71.36	344	2	3	5	NM_001042875.1	CG11327-RC, transcript variant C (CG11327), mRNA
0	0	2	3	4	NM_079470.2	CG10564-RA (Ac78C), mRNA
0	0	2	2	7	NM_001015159.1	CG12449-PB.3 (CG12449), mRNA
0	0	2	2	7	NM_001015162.1	CG12449-PF.3 (CG12449), mRNA
0	0	2	2	7	NM_001015158.1	CG12449-PD.3 (CG12449), mRNA
0	0	2	2	7	NM_001015157.1	CG12449-PA.3 (CG12449), mRNA
0	0	2	2	7	NM_001015161.1	CG12449-PE.3 (CG12449), mRNA
0	0	2	2	7	NM_001015160.1	CG12449-PC.3 (CG12449), mRNA
0	0	1	7	14	NM_134544.1	CG11734-RB (HERC2), mRNA
0	0	1	6	5	NM_135357.3	CG8183-RA, transcript variant A (Khc-73), mRNA
0	0	1	6	5	NM_176176.1	CG8183-RB, transcript variant B (Khc-73), mRNA
0	0	1	5	2	NM_137827.1	CG10344-RB, transcript variant B (CG10344), mRNA
0	0	1	5	2	NM_166534.1	CG10344-RA, transcript variant A (CG10344), mRNA
0	0	1	3	7	NM_164542.3	CG3234-RB, transcript variant B (tim), mRNA
0	0	1	3	7	NM_164540.2	CG3234-RD, transcript variant D (tim), mRNA
0	0	1	3	7	NM_001038783.1	CG3234-RF, transcript variant F (tim), mRNA
0	0	1	3	7	NM_001014463.1	CG3234-RE, transcript variant E (tim), mRNA
0	0	1	3	7	NM_078744.3	CG3234-RA, transcript variant A (tim), mRNA
0	0	1	3	6	NM_134892.2	CG17264-RA (CG17264), mRNA
0	0	1	3	4	NM_135977.1	CG15133-RA (CG15133), mRNA
0	0	1	2	5	NM_164541.3	CG3234-RC, transcript variant C (tim), mRNA
0	0	1	2	4	NM_057385.4	CG11121-RA (so), mRNA
0	0	1	2	3	NM_135762.2	CG16975-RB, transcript variant B (CG16975), mRNA
0	0	1	2	2	NM_170053.2	CG17894-RC, transcript variant C (cnc), mRNA
0	0	0	10	8	NM_057241.2	CG7210-RB, transcript variant B (kel), mRNA
0	0	0	10	8	NM_165242.1	CG7210-RA, transcript variant A (kel), mRNA
0	0	0	8	30	NM_079919.2	CG5700-RB (prc), mRNA
0	0	0	6	15	NM_132457.1	CG11122-RA (CG11122), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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