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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

11324R-3

Symbol

homer

Full Name

homer

CG No

CG11324

Old CG No

CG11324

Synonyms

Dm Homer, Dhomer, dhom, Dhom, LD03829, Dvh, CG11324, BEST:LD03829, homer, Homer

Accession No (Link to NCBI)

NM_058048.3

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATTTTCACCTGCCAGGCGCAC

Primer Seq. 3'

aatctagaggtaccGGACTGTCCGGATTGGCGTTC

Predicted Fragment Size

IR fragment full Seq

0001 ATTTTCACCT GCCAGGCGCA CGTCTTCCAC ATCGATCCGA ANGACGAAGC GCACCTGGAT 
0061 CACGGCCAGC ATGAAGGCGG TCAACGTTAG CTTCTTCTAC GACAGCTCGC GAAATCTGTA 
0121 CCGCATCATC AGTGTCGAGG GCACCAAGGC GGTGATAAAC TCGACGATCA CACCCAACAT 
0181 GACCTTCACC CAGACCTCCC AGAAGTTCGG CCAGTGGAGC GATGTGCGGG CAAACACGGT 
0241 GTACGGCTTG GGATTTGCCT CAGAGGCGGA ATTGACCAAG TTCGTAGAGA AGTTCCAGGA 
0301 GGTCAAGGAG GCCACCAAGA ATGCCATGAA ATCGGCCAAC GGATCGAATG CCGTCACGCC 
0361 CACCACCTCG GCGAACACGT CGCCCATTTC CGGAAGAGCT GTGGGCTCCA TGCAGAACGA 
0421 CAACACAGCC ATCGATCCGC ACACTGTGGA GCCGCCCAAC ATGAGCAACA CGAATACCCA 
0481 GAACGCCAAT CCGGACAGTC C

in silico PCR Fragment

0001 ATTTTCACCT GCCAGGCGCA CGTCTTCCAC ATCGATCCGA A-GACGAAGC GCACCTGGAT 
0061 CACGGCCAGC ATGAAGGCGG TCAACGTTAG CTTCTTCTAC GACAGCTCGC GAAATCTGTA 
0121 CCGCATCATC AGTGTCGAGG GCACCAAGGC GGTGATAAAC TCGACGATCA CACCCAACAT 
0181 GACCTTCACC CAGACCTCCC AGAAGTTCGG CCAGTGGAGC GATGTGCGGG CAAACACGGT 
0241 GTACGGCTTG GGATTTGCCT CAGAGGCGGA ATTGACCAAG TTCGTAGAGA AGTTCCAGGA 
0301 GGTCAAGGAG GCCACCAAGA ATGCCATGAA ATCGGCCAAC GGATCGAATG CCGTCACGCC 
0361 CACCACCTCG GCGAACACGT CGCCCATTTC CGGGAGAGCT GTGGGCTCCA TGCAGAACGA 
0421 CAACACAGCC ATCGATCCGC ACACTGTGGA GCCGCCCAAC ATGAGCAACA CGAATACCCA 
0481 GAACGCCAAT CCGGACAGTC C

Assemble Data

<PRE>

11324R-3.IR_full       1   ATTTTCACCTGCCAGGCGCACGTCTTCCACATCGATCCGAA<span class="snp"><tt>N</tt></span>GACGAAGCGCACCTGGAT 60
                           |||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||
11324R-3.in silico     1   ATTTTCACCTGCCAGGCGCACGTCTTCCACATCGATCCGAA<span class="snp"><tt>-</tt></span>GACGAAGCGCACCTGGAT 60


11324R-3.IR_full       61  CACGGCCAGCATGAAGGCGGTCAACGTTAGCTTCTTCTACGACAGCTCGCGAAATCTGTA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11324R-3.in silico     61  CACGGCCAGCATGAAGGCGGTCAACGTTAGCTTCTTCTACGACAGCTCGCGAAATCTGTA 120


11324R-3.IR_full       121 CCGCATCATCAGTGTCGAGGGCACCAAGGCGGTGATAAACTCGACGATCACACCCAACAT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11324R-3.in silico     121 CCGCATCATCAGTGTCGAGGGCACCAAGGCGGTGATAAACTCGACGATCACACCCAACAT 180


11324R-3.IR_full       181 GACCTTCACCCAGACCTCCCAGAAGTTCGGCCAGTGGAGCGATGTGCGGGCAAACACGGT 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11324R-3.in silico     181 GACCTTCACCCAGACCTCCCAGAAGTTCGGCCAGTGGAGCGATGTGCGGGCAAACACGGT 240


11324R-3.IR_full       241 GTACGGCTTGGGATTTGCCTCAGAGGCGGAATTGACCAAGTTCGTAGAGAAGTTCCAGGA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11324R-3.in silico     241 GTACGGCTTGGGATTTGCCTCAGAGGCGGAATTGACCAAGTTCGTAGAGAAGTTCCAGGA 300


11324R-3.IR_full       301 GGTCAAGGAGGCCACCAAGAATGCCATGAAATCGGCCAACGGATCGAATGCCGTCACGCC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11324R-3.in silico     301 GGTCAAGGAGGCCACCAAGAATGCCATGAAATCGGCCAACGGATCGAATGCCGTCACGCC 360


11324R-3.IR_full       361 CACCACCTCGGCGAACACGTCGCCCATTTCCGG<span class="snp"><tt>A</tt></span>AGAGCTGTGGGCTCCATGCAGAACGA 420
                           |||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||
11324R-3.in silico     361 CACCACCTCGGCGAACACGTCGCCCATTTCCGG<span class="snp"><tt>G</tt></span>AGAGCTGTGGGCTCCATGCAGAACGA 420


11324R-3.IR_full       421 CAACACAGCCATCGATCCGCACACTGTGGAGCCGCCCAACATGAGCAACACGAATACCCA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11324R-3.in silico     421 CAACACAGCCATCGATCCGCACACTGTGGAGCCGCCCAACATGAGCAACACGAATACCCA 480


11324R-3.IR_full       481 GAACGCCAATCCGGACAGTCC 501
                           |||||||||||||||||||||
11324R-3.in silico     481 GAACGCCAATCCGGACAGTCC 501

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_058048.3	CG11324-RA, transcript variant A (homer), mRNA
100	482	0	0	0	NM_001042876.1	CG11324-RC, transcript variant C (homer), mRNA
100	482	0	0	0	NM_164706.1	CG11324-RB, transcript variant B (homer), mRNA
0.2	1	2	6	2	NM_133158.2	CG12204-RA (CG12204), mRNA
0	0	6	6	3	NM_142520.2	CG6013-RA (CG6013), mRNA
0	0	3	9	3	NM_136597.3	CG8170-RA, transcript variant A (CG8170), mRNA
0	0	3	9	3	NM_001043068.1	CG8170-RB, transcript variant B (CG8170), mRNA
0	0	2	4	13	NM_167572.1	CG32555-RA, transcript variant A (RhoGAPp190), mRNA
0	0	2	4	13	NM_167573.1	CG32555-RC, transcript variant C (RhoGAPp190), mRNA
0	0	2	4	13	NM_133003.2	CG32555-RB, transcript variant B (RhoGAPp190), mRNA
0	0	2	2	6	NM_140492.2	CG17081-RA (CG17081), mRNA
0	0	2	2	5	NM_167185.1	CG32704-RA (CG32704), mRNA
0	0	2	2	2	NM_135602.2	CG6743-RA (Nup170), mRNA
0	0	1	8	8	NM_058025.3	CG4008-RA (und), mRNA
0	0	1	8	6	NM_057353.3	CG9668-RA (Rh4), mRNA
0	0	1	5	4	NM_139457.1	CG13801-RA (CG13801), mRNA
0	0	1	5	3	NM_176144.1	CG33144-RA (CG33144), mRNA
0	0	1	4	6	NM_132187.2	CG17256-RA (Nek2), mRNA
0	0	1	4	4	NM_165130.1	CG3903-RB, transcript variant B (Gli), mRNA
0	0	1	4	4	NM_057254.3	CG3903-RA, transcript variant A (Gli), mRNA
0	0	1	4	4	NM_165128.1	CG3903-RD, transcript variant D (Gli), mRNA
0	0	1	4	4	NM_165127.1	CG3903-RC, transcript variant C (Gli), mRNA
0	0	1	4	4	NM_165129.1	CG3903-RE, transcript variant E (Gli), mRNA
0	0	1	3	5	NM_079636.2	CG4898-RA, transcript variant A (Tm1), mRNA
0	0	1	3	5	NM_206494.1	CG4898-RL, transcript variant L (Tm1), mRNA
0	0	1	3	3	NM_135982.2	CG6304-RA (CG6304), mRNA
0	0	1	3	3	NM_134495.2	CG12234-RA (Ranbp21), mRNA
0	0	1	3	2	NM_139405.1	CG12020-RA (CG12020), mRNA
0	0	1	2	6	NM_080259.1	CG14066-RA, transcript variant A (larp), mRNA
0	0	1	2	6	NM_170366.1	CG14066-RB, transcript variant B (larp), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

dme04068 : FoxO signaling pathway

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