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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

11274R-3

Symbol

SRm160

Full Name

SRm160

CG No

CG11274

Old CG No

CG11274

Synonyms

CG11274, SRm160

Accession No (Link to NCBI)

NM_140370.1

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Fan YJ, Gittis AH, Juge F, Qiu C, Xu YZ, Rabinow L.<br> Multifunctional RNA processing protein SRm160 induces apoptosis and regulates eye and genital development in Drosophila.<br> Genetics (2014) 197(4) 1251-65 [ PubMed ID = 24907259 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGATGTTCACGGGCACCAATCA

Primer Seq. 3'

aatctagaggtaccACTTGGATTTGGAGCGGTCAC

Predicted Fragment Size

IR fragment full Seq

0001 GATGTTCACG GGCACCAATC AGCAGCAGGA CACGCGGTTC AGCGACAAGG AGAAGAAGTT 
0061 GATGAAGCAA ATGAAGTNTC GGCGATTGCC TGAACAAGAG GGTGGATATG TCGAAAGTCA 
0121 AGCTGGACGT ACTGCGTCCG TGGATCAGCA AGAAGATCAC CGACATCCTG CACATCGANG 
0181 GACGACGTGG TCGTCGAGNT TTGTCTACAA TCAGCTGGAG GAGGAGAAGT ATCCGTGCCC 
0241 CAAGAAAATG CAGATCAATA TGACGGGCTT CTTGAACGGC CGAAATGCCC GCCAGTTTAT 
0301 GGGTGAACTT TGGGCCCTGC TGCTTTCGGC ACAGGAAAGC GATTCGGGTA TACCAGCGGA 
0361 GTTTATACAG CAGAAAAAGG ACGAGATACT CAAACGGGAG GAGGAACAGC GGCAGCGGGA 
0421 TCGATCGAGA TCCCGTTCGC GTTCCCGCTC CCAACGAGAC AGTTCTAGGC GAGATCGGCA 
0481 GCGTGACCGC TCCAAATCCA AGT

in silico PCR Fragment

0001 GATGTTCACG GGCACCAATC AGCAGCAGGA CACGCGGTTC AGCGACAAGG AGAAGAAGCT 
0061 GATGAAGCAA ATGAAGT-TC GGCGATTGCC TGAACAAGAG GGTGGATATG TCGAAAGTCA 
0121 AGCTGGACGT ACTGCGTCCG TGGATCAGCA AGAAGATCAC CGACATCCTG CACATCGA-G 
0181 GACGACGTGG TCGTCGAG-T TTGTCTACAA TCAGCTGGAG GAGGAGAAGT ATCCGTGCCC 
0241 CAAGAAAATG CAGATCAATA TGACGGGCTT CTTGAACGGC CGAAATGCCC GCCAGTTTAT 
0301 GGGTGAACTT TGGGCCCTGC TGCTTTCGGC ACAGGAAAGC GATTCGGGTA TACCAGCGGA 
0361 GTTTATACAG CAGAAAAAGG ACGAGATACT CAAACGGGAG GAGGAACAGC GGCAGCGGGA 
0421 TCGATCGAGA TCCCGTTCGC GTTCCCGCTC CCAACGAGAC AGTTCTAGGC GAGATCGGCA 
0481 GCGTGACCGC TCCAAATCCA AGT

Assemble Data

<PRE>

11274R-3.IR_full       1   GATGTTCACGGGCACCAATCAGCAGCAGGACACGCGGTTCAGCGACAAGGAGAAGAAG<span class="snp"><tt>T</tt></span>T 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|
11274R-3.in silico     1   GATGTTCACGGGCACCAATCAGCAGCAGGACACGCGGTTCAGCGACAAGGAGAAGAAG<span class="snp"><tt>C</tt></span>T 60


11274R-3.IR_full       61  GATGAAGCAAATGAAGT<span class="snp"><tt>N</tt></span>TCGGCGATTGCCTGAACAAGAGGGTGGATATGTCGAAAGTCA 120
                           |||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||
11274R-3.in silico     61  GATGAAGCAAATGAAGT<span class="snp"><tt>-</tt></span>TCGGCGATTGCCTGAACAAGAGGGTGGATATGTCGAAAGTCA 120


11274R-3.IR_full       121 AGCTGGACGTACTGCGTCCGTGGATCAGCAAGAAGATCACCGACATCCTGCACATCGA<span class="snp"><tt>N</tt></span>G 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|
11274R-3.in silico     121 AGCTGGACGTACTGCGTCCGTGGATCAGCAAGAAGATCACCGACATCCTGCACATCGA<span class="snp"><tt>-</tt></span>G 180


11274R-3.IR_full       181 GACGACGTGGTCGTCGAG<span class="snp"><tt>N</tt></span>TTTGTCTACAATCAGCTGGAGGAGGAGAAGTATCCGTGCCC 240
                           ||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||
11274R-3.in silico     181 GACGACGTGGTCGTCGAG<span class="snp"><tt>-</tt></span>TTTGTCTACAATCAGCTGGAGGAGGAGAAGTATCCGTGCCC 240


11274R-3.IR_full       241 CAAGAAAATGCAGATCAATATGACGGGCTTCTTGAACGGCCGAAATGCCCGCCAGTTTAT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11274R-3.in silico     241 CAAGAAAATGCAGATCAATATGACGGGCTTCTTGAACGGCCGAAATGCCCGCCAGTTTAT 300


11274R-3.IR_full       301 GGGTGAACTTTGGGCCCTGCTGCTTTCGGCACAGGAAAGCGATTCGGGTATACCAGCGGA 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11274R-3.in silico     301 GGGTGAACTTTGGGCCCTGCTGCTTTCGGCACAGGAAAGCGATTCGGGTATACCAGCGGA 360


11274R-3.IR_full       361 GTTTATACAGCAGAAAAAGGACGAGATACTCAAACGGGAGGAGGAACAGCGGCAGCGGGA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11274R-3.in silico     361 GTTTATACAGCAGAAAAAGGACGAGATACTCAAACGGGAGGAGGAACAGCGGCAGCGGGA 420


11274R-3.IR_full       421 TCGATCGAGATCCCGTTCGCGTTCCCGCTCCCAACGAGACAGTTCTAGGCGAGATCGGCA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11274R-3.in silico     421 TCGATCGAGATCCCGTTCGCGTTCCCGCTCCCAACGAGACAGTTCTAGGCGAGATCGGCA 480


11274R-3.IR_full       481 GCGTGACCGCTCCAAATCCAAGT 503
                           |||||||||||||||||||||||
11274R-3.in silico     481 GCGTGACCGCTCCAAATCCAAGT 503

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	3	12	NM_140370.1	CG11274-RA (SRm160), mRNA
0.2	1	6	2	4	NM_168126.1	CG5406-RB, transcript variant B (sif), mRNA
0.2	1	3	3	3	NM_142882.1	CG17083-RA (CG17083), mRNA
0.2	1	3	2	4	NM_135685.2	CG6686-RB, transcript variant B (CG6686), mRNA
0.2	1	3	2	4	NM_164977.1	CG6686-RA, transcript variant A (CG6686), mRNA
0	0	9	4	3	NM_057576.3	CG6407-RA (Wnt5), mRNA
0	0	6	4	10	NM_139949.2	CG7185-RA (CG7185), mRNA
0	0	4	5	17	NM_164887.1	CG31714-RA (CG31714), mRNA
0	0	3	10	32	NM_132858.2	CG9056-RA (CG9056), mRNA
0	0	3	7	3	NM_141408.3	CG1137-RA (CG1137), mRNA
0	0	3	2	8	NM_170388.1	CG11876-RC, transcript variant C (CG11876), mRNA
0	0	3	2	8	NM_170387.1	CG11876-RB, transcript variant B (CG11876), mRNA
0	0	3	2	7	NM_138049.2	CG3328-RA (CG3328), mRNA
0	0	2	6	16	NM_206773.1	CG32556-RB, transcript variant B (CG32556), mRNA
0	0	2	6	14	NM_167574.1	CG32556-RA, transcript variant A (CG32556), mRNA
0	0	2	6	6	NM_139874.1	CG7548-RA (CG7548), mRNA
0	0	2	5	14	NM_134516.2	CG32529-RA, transcript variant A (CG32529), mRNA
0	0	2	4	5	NM_058105.4	CG7697-RA (CstF-64), mRNA
0	0	2	4	3	NM_057516.3	CG5403-RA, transcript variant A (retn), mRNA
0	0	2	4	3	NM_176254.1	CG5403-RB, transcript variant B (retn), mRNA
0	0	2	3	4	NM_001014484.1	CG15279-RC, transcript variant C (CG15279), mRNA
0	0	2	3	4	NM_140499.2	CG6876-RA (CG6876), mRNA
0	0	2	3	4	NM_135882.2	CG15279-RA, transcript variant A (CG15279), mRNA
0	0	2	3	4	NM_165093.1	CG15279-RB, transcript variant B (CG15279), mRNA
0	0	2	2	4	NM_167121.1	CG9650-RC, transcript variant C (CG9650), mRNA
0	0	2	2	4	NM_132157.1	CG9650-RB, transcript variant B (CG9650), mRNA
0	0	2	2	4	NM_167123.2	CG9650-RA, transcript variant A (CG9650), mRNA
0	0	2	2	3	NM_136854.1	CG13194-RA (pyr), mRNA
0	0	2	2	3	NM_167916.2	CG32315-RA (dlt), mRNA
0	0	1	8	36	NM_133111.1	CG7358-RA (CG7358), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

dme03013 : Nucleocytoplasmic transport
dme03015 : mRNA surveillance pathway

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