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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
11263R-2 |
| Symbol |
CG11263 |
Full Name |
CG11263 |
| CG No |
CG11263 |
Old CG No |
CG11263 |
| Synonyms |
CG11263 |
| Accession No (Link to NCBI) |
NM_140361.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Yang Z, Chen KM, Pandey RR, Homolka D, Reuter M, Janeiro BK, Sachidanandam R, Fauvarque MO, McCarthy AA, Pillai RS.<br> PIWI Slicing and EXD1 Drive Biogenesis of Nuclear piRNAs from Cytosolic Targets of the Mouse piRNA Pathway.<br> Mol Cell (2016) 61(1) 138-52
[
PubMed ID = 26669262
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCCTGGAGAAGCAACTAGATCG |
| Primer Seq. 3' |
aatctagaggtaccCCAACTTGGCCAAGTATCTCA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CCTGGAGAAG CAACTAGATC GAATTGTTTT GATATACCAG GTGGATACCA CATACCACAG
0061 TGCACTAAAG GACATAAAGG ATCAGAAGAT CATATCGCTG CTGGTGGAGC CCAGTTTTTA
0121 CGGACGACAT CACCCCACGT CTATTTTGGT GGTAGCCACG TGCAATGGAA CGTACATTTT
0181 CGACATCAAG GCCCTTGGCT TGATATTTCT GGAACTGGCC AAGATTCTGG AGGCGGATCA
0241 GCCGCGCNAA GGTAATCCAC TACAGCCACC GGATCGCCGA TCATCTGTTG CATANGGCAA
0301 CGCATCTCGT TGGGAGGAAT ATTTGATACC TTTGTGGCTC TATGCCTGAG CAGCAACACA
0361 AGAATTCCGT ACACCTTGCC AGAGGCTATA TCTCTCGTCT TCGGACTACC CATGGAGAAG
0421 GTGACTGGAG GCTGTGAGTC GCAGCGTAAC TTCACTGCTC GTCCACTGAC CCACAGTCAA
0481 ATGAGATACT TGGCCAAGTT GG |
| in silico PCR Fragment |
0001 CCTGGAGAAG CAACTAGATC GAATTGTTTT GATATACCAG GTGGATACCA CATACCACAG
0061 TGCACTAAAG GACATAAAGG ATCAGAAGAT CATATCGCTG CTGGTGGAGC CCAGTTTTTA
0121 CGGACGACAT CACCCCACGT CTATTTTGGT GGTAGCCACG TGCAATGGAA CGTACATTTT
0181 CGACATCAAG GCCCTTGGCT TGATATTTCT GGAACTGGCC AAGATTCTGG AGGCGGATCA
0241 GCCGCGC-AA GGTAATCCAC TACAGCCACC GGATCGCCGA TCATCTGTTG CATA-GGCAA
0301 CGCATCTCGT TGGGAGGAAT ATTTGATACC TTTGTGGCTG TATGCCTGAG CAGCAACACA
0361 AGAATTCCGT ACACCTTGCC AGAGGCTATA TCTCTCGTCT TCGGACTACC CATGGAGAAG
0421 GTGACTGGAG GCTGTGAGTC GCAGCGTAAC TTCACTGCTC GTCCACTGAC CCACAGTCAA
0481 ATGAGATACT TGGCCAAGTT GG |
| Assemble Data |
<PRE>
11263R-2.IR_full 1 CCTGGAGAAGCAACTAGATCGAATTGTTTTGATATACCAGGTGGATACCACATACCACAG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11263R-2.in silico 1 CCTGGAGAAGCAACTAGATCGAATTGTTTTGATATACCAGGTGGATACCACATACCACAG 60
11263R-2.IR_full 61 TGCACTAAAGGACATAAAGGATCAGAAGATCATATCGCTGCTGGTGGAGCCCAGTTTTTA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11263R-2.in silico 61 TGCACTAAAGGACATAAAGGATCAGAAGATCATATCGCTGCTGGTGGAGCCCAGTTTTTA 120
11263R-2.IR_full 121 CGGACGACATCACCCCACGTCTATTTTGGTGGTAGCCACGTGCAATGGAACGTACATTTT 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11263R-2.in silico 121 CGGACGACATCACCCCACGTCTATTTTGGTGGTAGCCACGTGCAATGGAACGTACATTTT 180
11263R-2.IR_full 181 CGACATCAAGGCCCTTGGCTTGATATTTCTGGAACTGGCCAAGATTCTGGAGGCGGATCA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11263R-2.in silico 181 CGACATCAAGGCCCTTGGCTTGATATTTCTGGAACTGGCCAAGATTCTGGAGGCGGATCA 240
11263R-2.IR_full 241 GCCGCGC<span class="snp"><tt>N</tt></span>AAGGTAATCCACTACAGCCACCGGATCGCCGATCATCTGTTGCATA<span class="snp"><tt>N</tt></span>GGCAA 300
|||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||
11263R-2.in silico 241 GCCGCGC<span class="snp"><tt>-</tt></span>AAGGTAATCCACTACAGCCACCGGATCGCCGATCATCTGTTGCATA<span class="snp"><tt>-</tt></span>GGCAA 300
11263R-2.IR_full 301 CGCATCTCGTTGGGAGGAATATTTGATACCTTTGTGGCT<span class="snp"><tt>C</tt></span>TATGCCTGAGCAGCAACACA 360
|||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||
11263R-2.in silico 301 CGCATCTCGTTGGGAGGAATATTTGATACCTTTGTGGCT<span class="snp"><tt>G</tt></span>TATGCCTGAGCAGCAACACA 360
11263R-2.IR_full 361 AGAATTCCGTACACCTTGCCAGAGGCTATATCTCTCGTCTTCGGACTACCCATGGAGAAG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11263R-2.in silico 361 AGAATTCCGTACACCTTGCCAGAGGCTATATCTCTCGTCTTCGGACTACCCATGGAGAAG 420
11263R-2.IR_full 421 GTGACTGGAGGCTGTGAGTCGCAGCGTAACTTCACTGCTCGTCCACTGACCCACAGTCAA 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11263R-2.in silico 421 GTGACTGGAGGCTGTGAGTCGCAGCGTAACTTCACTGCTCGTCCACTGACCCACAGTCAA 480
11263R-2.IR_full 481 ATGAGATACTTGGCCAAGTTGG 502
||||||||||||||||||||||
11263R-2.in silico 481 ATGAGATACTTGGCCAAGTTGG 502
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_140361.1 |
CG11263-RA (CG11263), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_170036.1 |
CG31239-RA (CG31239), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_142947.2 |
CG5933-RA (CG5933), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_132760.3 |
CG9009-RA (CG9009), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_167164.2 |
CG12737-RB, transcript variant B (Crag), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_080341.2 |
CG12737-RA, transcript variant A (Crag), mRNA |
| 0 |
0 |
1 |
7 |
3 |
NM_142581.1 |
CG4562-RA (CG4562), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_132616.1 |
CG15721-RA (CG15721), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_139928.3 |
CG7999-RA (MED24), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_136483.2 |
CG12822-RA, transcript variant A (CG12822), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_206052.1 |
CG12822-RB, transcript variant B (CG12822), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_140431.2 |
CG6451-RA (blue), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_080340.2 |
CG2302-RA (nAcRalpha-7E), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_080189.2 |
CG16880-RA (l(2)34Fa), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_140447.1 |
CG9598-RA (CG9598), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_144328.2 |
CG10570-RA (CG10570), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_164377.1 |
CG3645-RB, transcript variant B (CG3645), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_134663.2 |
CG3645-RA, transcript variant A (CG3645), mRNA |
| 0 |
0 |
0 |
5 |
7 |
NM_057406.3 |
CG6222-RA (su(s)), mRNA |
| 0 |
0 |
0 |
5 |
7 |
NM_176542.1 |
CG7029-RA (CG7029), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_001014545.1 |
CG33519-RB (Unc-89), mRNA |
| 0 |
0 |
0 |
4 |
7 |
NM_057604.3 |
CG1825-RA (Map60), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_206019.1 |
CG9270-RB, transcript variant B (CG9270), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_136235.2 |
CG9270-RA, transcript variant A (CG9270), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_142676.2 |
CG11622-RA (Rlip), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_140439.2 |
CG5842-RA (nan), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_166669.1 |
CG4527-RB, transcript variant B (slik), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_206214.1 |
CG4527-RC, transcript variant C (slik), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_206213.1 |
CG4527-RD, transcript variant D (slik), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_136155.2 |
CG10628-RA (CG10628), mRNA |
|
| Wing Disc |
GFP
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Puc
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Caspase
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DAPI
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Merge
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Figure
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JNK signal
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Caspase-3 signal
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Pathways (updated: 2024/04/26)
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| KEGG Pathway |
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