NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

11253R-2

Symbol

CG11253

Full Name

CG11253

CG No

CG11253

Old CG No

CG11253

Synonyms

CG11253

Accession No (Link to NCBI)

NM_140368.1

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGAATCGCGAGGAGGAGGTGAA

Primer Seq. 3'

aatctagaggtaccTGAGCCACGGAATGTCATGGG

Predicted Fragment Size

IR fragment full Seq

0001 GAATCGCGAG GAGGAGGTGA AGGAGTTCCT CATCTCGCGT GACAAGCTAC GCGTNGCTGA 
0061 TCCACGAAGC CTATTGCGTA ACGTTGTGGA AGACGCGTGT TCTGCCGCAT CTGCTGGAGA 
0121 TTGATCCCAA TCCGCAGGCC ACGTTCCTTA TCTACACGGT GCTATATCAC GAGGCTGCTT 
0181 TGGTAGCGCT TCTGGATATG TGTCTCTACC ATCCCAGTGG CTGTGAGACG CTGCAGGAGT 
0241 CCGTGCTGGA TTTGATTGAC TATTGCGCCC AGGCCATTTC CCAGGTCATT GGCTTGGTCA 
0301 GCATGGGTTA CCACGAGAAC GAGACAAAGC TGGACGTGGA TGAGGCAGTG CTCACGGAGC 
0361 TGGAGCGCCA GAAGCGCGAC TTNTATCTAT AAGATTGGAC TGCGCTGCAT CTCAGTGCTG 
0421 AACTACATAG CAGATAATGT GACATTGTTT CATCTAAgNG CCGCACGCCG TTTGCTGGTG 
0481 ACCCATGACA TTCCGTGGCT CA

in silico PCR Fragment

0001 GAATCGCGAG GAGGAGGTGA AGGAGTTCCT CATCTCGCGC GACAAGCTAC GCGT-GCTGA 
0061 TCCACGAAGC CTATTGCGTA ACGTTGTGGA AGACGCGTGT TCTGCCGCAT CTGCTGGAGA 
0121 TTGATCCCAA TCCGCAGGCC ACGTTCCTTA TCTACACGGT GCTATATCAC GAGGCTGCTT 
0181 TGGTAGCGCT TCTGGATGTG TGCCTCTACC ATCCGAGTGG CTGTGAGACG CTGCAGGAGT 
0241 CCGTGCTGGA TTTGATTGAC TATTGCGCCC AGGCCATTTC CCAGGTCATT GGCTTGGTTA 
0301 GCATGGGTTA CCACGAGAAC GAGACAAAGC TGGACGTGGA TGAGGCAGTG CTCACGGAGC 
0361 TGGAGCGCCA GAAGCGCGAC TT-TATCTAT AAGATTGGAC TGCGCTGCAT CTCAGTGCTG 
0421 AACTACATAG CAGATAATGT GACATTGTTT CATCTAAGCG CCGCACGCCG TTTGCTGGTG 
0481 ACCCATGACA TTCCGTGGCT CA

Assemble Data

<PRE>

11253R-2.IR_full       1   GAATCGCGAGGAGGAGGTGAAGGAGTTCCTCATCTCGCG<span class="snp"><tt>T</tt></span>GACAAGCTACGCGT<span class="snp"><tt>N</tt></span>GCTGA 60
                           |||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||
11253R-2.in silico     1   GAATCGCGAGGAGGAGGTGAAGGAGTTCCTCATCTCGCG<span class="snp"><tt>C</tt></span>GACAAGCTACGCGT<span class="snp"><tt>-</tt></span>GCTGA 60


11253R-2.IR_full       61  TCCACGAAGCCTATTGCGTAACGTTGTGGAAGACGCGTGTTCTGCCGCATCTGCTGGAGA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11253R-2.in silico     61  TCCACGAAGCCTATTGCGTAACGTTGTGGAAGACGCGTGTTCTGCCGCATCTGCTGGAGA 120


11253R-2.IR_full       121 TTGATCCCAATCCGCAGGCCACGTTCCTTATCTACACGGTGCTATATCACGAGGCTGCTT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11253R-2.in silico     121 TTGATCCCAATCCGCAGGCCACGTTCCTTATCTACACGGTGCTATATCACGAGGCTGCTT 180


11253R-2.IR_full       181 TGGTAGCGCTTCTGGAT<span class="snp"><tt>A</tt></span>TGTG<span class="snp"><tt>T</tt></span>CTCTACCATCC<span class="snp"><tt>C</tt></span>AGTGGCTGTGAGACGCTGCAGGAGT 240
                           |||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||
11253R-2.in silico     181 TGGTAGCGCTTCTGGAT<span class="snp"><tt>G</tt></span>TGTG<span class="snp"><tt>C</tt></span>CTCTACCATCC<span class="snp"><tt>G</tt></span>AGTGGCTGTGAGACGCTGCAGGAGT 240


11253R-2.IR_full       241 CCGTGCTGGATTTGATTGACTATTGCGCCCAGGCCATTTCCCAGGTCATTGGCTTGGT<span class="snp"><tt>C</tt></span>A 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|
11253R-2.in silico     241 CCGTGCTGGATTTGATTGACTATTGCGCCCAGGCCATTTCCCAGGTCATTGGCTTGGT<span class="snp"><tt>T</tt></span>A 300


11253R-2.IR_full       301 GCATGGGTTACCACGAGAACGAGACAAAGCTGGACGTGGATGAGGCAGTGCTCACGGAGC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11253R-2.in silico     301 GCATGGGTTACCACGAGAACGAGACAAAGCTGGACGTGGATGAGGCAGTGCTCACGGAGC 360


11253R-2.IR_full       361 TGGAGCGCCAGAAGCGCGACTT<span class="snp"><tt>N</tt></span>TATCTATAAGATTGGACTGCGCTGCATCTCAGTGCTG 420
                           ||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||
11253R-2.in silico     361 TGGAGCGCCAGAAGCGCGACTT<span class="snp"><tt>-</tt></span>TATCTATAAGATTGGACTGCGCTGCATCTCAGTGCTG 420


11253R-2.IR_full       421 AACTACATAGCAGATAATGTGACATTGTTTCATCTAAG<span class="snp"><tt>N</tt></span>GCCGCACGCCGTTTGCTGGTG 480
                           ||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||
11253R-2.in silico     421 AACTACATAGCAGATAATGTGACATTGTTTCATCTAAG<span class="snp"><tt>C</tt></span>GCCGCACGCCGTTTGCTGGTG 480


11253R-2.IR_full       481 ACCCATGACATTCCGTGGCTCA 502
                           ||||||||||||||||||||||
11253R-2.in silico     481 ACCCATGACATTCCGTGGCTCA 502

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	1	NM_140368.1	CG11253-RA (CG11253), mRNA
0.2	1	2	4	2	NM_132765.1	CG5548-RA (CG5548), mRNA
0	0	6	13	50	NM_167319.3	CG32656-RA (CG32656), mRNA
0	0	4	5	2	NM_138052.2	CG3356-RA (CG3356), mRNA
0	0	4	4	2	NM_057666.3	CG10922-RA, transcript variant A (La), mRNA
0	0	4	4	2	NM_165326.1	CG10922-RB, transcript variant B (La), mRNA
0	0	4	3	4	NM_136580.1	CG8243-RA (CG8243), mRNA
0	0	4	2	2	NM_079230.2	CG32386-RA (corn), mRNA
0	0	3	2	2	NM_142174.1	CG6623-RA (CG6623), mRNA
0	0	2	5	2	NM_001042868.1	CG34124-RA (CG34124), mRNA
0	0	2	4	7	NM_165629.1	CG30350-RA (CG30350), mRNA
0	0	2	4	3	NM_132826.2	CG9240-RA (CG9240), mRNA
0	0	2	3	8	NM_168571.2	CG32133-RA (CG32133), mRNA
0	0	2	3	7	NM_142225.2	CG5404-RA (CG5404), mRNA
0	0	2	3	3	NM_169588.1	CG31317-RA, transcript variant A (stumps), mRNA
0	0	2	3	3	NM_169589.1	CG31317-RB, transcript variant B (stumps), mRNA
0	0	2	3	3	NM_167190.1	CG15316-RB, transcript variant B (CG15316), mRNA
0	0	2	3	3	NM_132338.1	CG15316-RA, transcript variant A (CG15316), mRNA
0	0	2	2	5	NM_205934.1	CG13391-RB, transcript variant B (Aats-ala), mRNA
0	0	2	2	5	NM_078787.2	CG13391-RA, transcript variant A (Aats-ala), mRNA
0	0	2	2	2	NM_167003.1	CG32775-RA (GlcAT-I), mRNA
0	0	1	6	6	NM_139554.3	CG12076-RA, transcript variant A (YT521-B), mRNA
0	0	1	6	6	NM_168029.1	CG12076-RB, transcript variant B (YT521-B), mRNA
0	0	1	6	3	NM_135024.1	CG11926-RA (CG11926), mRNA
0	0	1	6	2	NM_167445.2	CG6294-RA (CG6294), mRNA
0	0	1	5	3	NM_079468.2	CG10582-RA (Sin), mRNA
0	0	1	4	5	NM_079700.1	CG3723-RA (Dhc93AB), mRNA
0	0	1	3	5	NM_078800.2	CG4422-RA (Gdi), mRNA
0	0	1	3	3	NM_169803.3	CG7125-RB, transcript variant B (PKD), mRNA
0	0	1	3	3	NM_169804.3	CG7125-RC, transcript variant C (PKD), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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