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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
11253R-1 |
| Symbol |
CG11253 |
Full Name |
CG11253 |
| CG No |
CG11253 |
Old CG No |
CG11253 |
| Synonyms |
CG11253 |
| Accession No (Link to NCBI) |
NM_140368.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
semi-lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGAATCGCGAGGAGGAGGTGAA |
| Primer Seq. 3' |
aatctagaggtaccTGAGCCACGGAATGTCATGGG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GAATCGCGAG GAGGAGGTGA AGGAGTTCCT CATCTCGCGT GACAAGCTAC GCGTNGCTGA
0061 TCCACGAAGC CTATTGCGTA ACGTTGTGGA AGACGCGTGT TCTGCCGCAT CTGCTGGAGA
0121 TTGATCCCAA TCCGCAGGCC ACGTTCCTTA TCTACACGGT GCTATATCAC GAGGCTGCTT
0181 TGGTAGCGCT TCTGGATATG TGTCTCTACC ATCCCAGTGG CTGTGAGACG CTGCAGGAGT
0241 CCGTGCTGGA TTTGATTGAC TATTGCGCCC AGGCCATTTC CCAGGTCATT GGCTTGGTCA
0301 GCATGGGTTA CCACGAGAAC GAGACAAAGC TGGACGTGGA TGAGGCAGTG CTCACGGAGC
0361 TGGAGCGCCA GAAGCGCGAC TTNTATCTAT AAGATTGGAC TGCGCTGCAT CTCAGTGCTG
0421 AACTACATAG CAGATAATGT GACATTGTTT CATCTAAgNG CCGCACGCCG TTTGCTGGTG
0481 ACCCATGACA TTCCGTGGCT CA |
| in silico PCR Fragment |
0001 GAATCGCGAG GAGGAGGTGA AGGAGTTCCT CATCTCGCGC GACAAGCTAC GCGT-GCTGA
0061 TCCACGAAGC CTATTGCGTA ACGTTGTGGA AGACGCGTGT TCTGCCGCAT CTGCTGGAGA
0121 TTGATCCCAA TCCGCAGGCC ACGTTCCTTA TCTACACGGT GCTATATCAC GAGGCTGCTT
0181 TGGTAGCGCT TCTGGATGTG TGCCTCTACC ATCCGAGTGG CTGTGAGACG CTGCAGGAGT
0241 CCGTGCTGGA TTTGATTGAC TATTGCGCCC AGGCCATTTC CCAGGTCATT GGCTTGGTTA
0301 GCATGGGTTA CCACGAGAAC GAGACAAAGC TGGACGTGGA TGAGGCAGTG CTCACGGAGC
0361 TGGAGCGCCA GAAGCGCGAC TT-TATCTAT AAGATTGGAC TGCGCTGCAT CTCAGTGCTG
0421 AACTACATAG CAGATAATGT GACATTGTTT CATCTAAGCG CCGCACGCCG TTTGCTGGTG
0481 ACCCATGACA TTCCGTGGCT CA |
| Assemble Data |
<PRE>
11253R-1.IR_full 1 GAATCGCGAGGAGGAGGTGAAGGAGTTCCTCATCTCGCG<span class="snp"><tt>T</tt></span>GACAAGCTACGCGT<span class="snp"><tt>N</tt></span>GCTGA 60
|||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||<span class="snp"><tt> </tt></span>|||||
11253R-1.in silico 1 GAATCGCGAGGAGGAGGTGAAGGAGTTCCTCATCTCGCG<span class="snp"><tt>C</tt></span>GACAAGCTACGCGT<span class="snp"><tt>-</tt></span>GCTGA 60
11253R-1.IR_full 61 TCCACGAAGCCTATTGCGTAACGTTGTGGAAGACGCGTGTTCTGCCGCATCTGCTGGAGA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11253R-1.in silico 61 TCCACGAAGCCTATTGCGTAACGTTGTGGAAGACGCGTGTTCTGCCGCATCTGCTGGAGA 120
11253R-1.IR_full 121 TTGATCCCAATCCGCAGGCCACGTTCCTTATCTACACGGTGCTATATCACGAGGCTGCTT 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11253R-1.in silico 121 TTGATCCCAATCCGCAGGCCACGTTCCTTATCTACACGGTGCTATATCACGAGGCTGCTT 180
11253R-1.IR_full 181 TGGTAGCGCTTCTGGAT<span class="snp"><tt>A</tt></span>TGTG<span class="snp"><tt>T</tt></span>CTCTACCATCC<span class="snp"><tt>C</tt></span>AGTGGCTGTGAGACGCTGCAGGAGT 240
|||||||||||||||||<span class="snp"><tt> </tt></span>||||<span class="snp"><tt> </tt></span>|||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||
11253R-1.in silico 181 TGGTAGCGCTTCTGGAT<span class="snp"><tt>G</tt></span>TGTG<span class="snp"><tt>C</tt></span>CTCTACCATCC<span class="snp"><tt>G</tt></span>AGTGGCTGTGAGACGCTGCAGGAGT 240
11253R-1.IR_full 241 CCGTGCTGGATTTGATTGACTATTGCGCCCAGGCCATTTCCCAGGTCATTGGCTTGGT<span class="snp"><tt>C</tt></span>A 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|
11253R-1.in silico 241 CCGTGCTGGATTTGATTGACTATTGCGCCCAGGCCATTTCCCAGGTCATTGGCTTGGT<span class="snp"><tt>T</tt></span>A 300
11253R-1.IR_full 301 GCATGGGTTACCACGAGAACGAGACAAAGCTGGACGTGGATGAGGCAGTGCTCACGGAGC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11253R-1.in silico 301 GCATGGGTTACCACGAGAACGAGACAAAGCTGGACGTGGATGAGGCAGTGCTCACGGAGC 360
11253R-1.IR_full 361 TGGAGCGCCAGAAGCGCGACTT<span class="snp"><tt>N</tt></span>TATCTATAAGATTGGACTGCGCTGCATCTCAGTGCTG 420
||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||||
11253R-1.in silico 361 TGGAGCGCCAGAAGCGCGACTT<span class="snp"><tt>-</tt></span>TATCTATAAGATTGGACTGCGCTGCATCTCAGTGCTG 420
11253R-1.IR_full 421 AACTACATAGCAGATAATGTGACATTGTTTCATCTAAG<span class="snp"><tt>N</tt></span>GCCGCACGCCGTTTGCTGGTG 480
||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||
11253R-1.in silico 421 AACTACATAGCAGATAATGTGACATTGTTTCATCTAAG<span class="snp"><tt>C</tt></span>GCCGCACGCCGTTTGCTGGTG 480
11253R-1.IR_full 481 ACCCATGACATTCCGTGGCTCA 502
||||||||||||||||||||||
11253R-1.in silico 481 ACCCATGACATTCCGTGGCTCA 502
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
1 |
NM_140368.1 |
CG11253-RA (CG11253), mRNA |
| 0.2 |
1 |
2 |
4 |
2 |
NM_132765.1 |
CG5548-RA (CG5548), mRNA |
| 0 |
0 |
6 |
13 |
50 |
NM_167319.3 |
CG32656-RA (CG32656), mRNA |
| 0 |
0 |
4 |
5 |
2 |
NM_138052.2 |
CG3356-RA (CG3356), mRNA |
| 0 |
0 |
4 |
4 |
2 |
NM_057666.3 |
CG10922-RA, transcript variant A (La), mRNA |
| 0 |
0 |
4 |
4 |
2 |
NM_165326.1 |
CG10922-RB, transcript variant B (La), mRNA |
| 0 |
0 |
4 |
3 |
4 |
NM_136580.1 |
CG8243-RA (CG8243), mRNA |
| 0 |
0 |
4 |
2 |
2 |
NM_079230.2 |
CG32386-RA (corn), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_142174.1 |
CG6623-RA (CG6623), mRNA |
| 0 |
0 |
2 |
5 |
2 |
NM_001042868.1 |
CG34124-RA (CG34124), mRNA |
| 0 |
0 |
2 |
4 |
7 |
NM_165629.1 |
CG30350-RA (CG30350), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_132826.2 |
CG9240-RA (CG9240), mRNA |
| 0 |
0 |
2 |
3 |
8 |
NM_168571.2 |
CG32133-RA (CG32133), mRNA |
| 0 |
0 |
2 |
3 |
7 |
NM_142225.2 |
CG5404-RA (CG5404), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_169588.1 |
CG31317-RA, transcript variant A (stumps), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_169589.1 |
CG31317-RB, transcript variant B (stumps), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_167190.1 |
CG15316-RB, transcript variant B (CG15316), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_132338.1 |
CG15316-RA, transcript variant A (CG15316), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_205934.1 |
CG13391-RB, transcript variant B (Aats-ala), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_078787.2 |
CG13391-RA, transcript variant A (Aats-ala), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_167003.1 |
CG32775-RA (GlcAT-I), mRNA |
| 0 |
0 |
1 |
6 |
6 |
NM_139554.3 |
CG12076-RA, transcript variant A (YT521-B), mRNA |
| 0 |
0 |
1 |
6 |
6 |
NM_168029.1 |
CG12076-RB, transcript variant B (YT521-B), mRNA |
| 0 |
0 |
1 |
6 |
3 |
NM_135024.1 |
CG11926-RA (CG11926), mRNA |
| 0 |
0 |
1 |
6 |
2 |
NM_167445.2 |
CG6294-RA (CG6294), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_079468.2 |
CG10582-RA (Sin), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_079700.1 |
CG3723-RA (Dhc93AB), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_078800.2 |
CG4422-RA (Gdi), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_169803.3 |
CG7125-RB, transcript variant B (PKD), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_169804.3 |
CG7125-RC, transcript variant C (PKD), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| +- |
|
| DB |
 |
| +- |
|
| DC |
 |
| + |
|
| DM |
 |
| +- |
|
| VM |
 |
| +- |
|
| VB |
 |
| |
|
|
Caspase-3 signal
| N |
|
| + |
|
| DB |
|
| + |
|
| DC |
|
| ++ |
|
| DM |
|
| + |
|
| VM |
|
| + |
|
| VB |
|
| |
|
|
|
|
|
Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
|
|
|
