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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

11251R-1

Symbol

CG11251

Full Name

CG11251

CG No

CG11251

Old CG No

CG11251

Synonyms

CG11251

Accession No (Link to NCBI)

NM_140375.1

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgACCTCCTTCGCAACTTCATTC

Primer Seq. 3'

aatctagaggtaccGTTGTCCAGAGCTCCGGTTAT

Predicted Fragment Size

IR fragment full Seq

0001 ACCTCCTTCG CAACTTCATT CCCAAGTGGT GTCTCCCACG TCCAGAGAAG CATCTCCAGA 
0061 ACCACATATT CCGTCAGCTC CAGACGAATC ACCCATCCGC CGCACTGGTG GCCATCAAAA 
0121 CAAGTGAACA GGTGCTGCCC AAGTGCAGAT TCATGCAGTA CCGGCGCGCC AATGAGCAGG 
0181 TGAAGCGATT GGGCATGGTC TCCGAGGATG GCAGCAAGAT GGCGGAGCTA NTCGAGCATG 
0241 ACCTGCGCCG CCCCCAATAT GATGGACTTC ATCCAGCAGA GATACTGCAT GGTTAGCCTG 
0301 CTGGACAGTG TGCAATTCAT GAAGATCGAG GATGTGGAGG CCGTGGATCT CCGCCTGCTG 
0361 CCACCCATCG ATTCCCCCGG TAAGATCATT GGCGTGGACT GCAACTATGT GGACAACTGT 
0421 GATGAGCAGC ACATCTCCAT TCCGAGGGAG CCGAGCTTTC ACGTCAAGTT CGCCTCCTCG 
0481 ATAACCGGAG CTCTGGACAA C

in silico PCR Fragment

0001 ACCTCCTTCG CAACTTCATT CCCAAGTGGT GTCTCCCACG TCCAGAGAAG CATCTCCAGA 
0061 ACCACATATT CCGTCAGCTC CAGACGAATC ACCCATCCGC CGCACTGGTG GCCATCAAAA 
0121 CAAGTGAACA GGTGCTGCCC AAGTGCAGAT TCATGCAGTA CCGGCGCGCC AATGAGCAGG 
0181 TGAAGCGATT GGGCATGGTC TCCGAGGATG GCAGCAAGAT GGCGGAGCTA -TCGAGCATG 
0241 ACCTGCGCCG CCCCCAATAT GATGGACTTC ATCCAGCAGA GATACTGCAT GGTTAGCCTG 
0301 CTGGACAGTG TGCAATTCAT GAAGATCGAG GATGTGGAGG CCGTGGATCT CCGCCTGCTG 
0361 CCACCCATCG ATTCCCCCAG TAAGATCATT GGCGTGGACT GCAACTATGT GGATAACTGT 
0421 GATGAGCAGC ACATCTCGAT TCCGAGGGAG CCGAGTTTTC ACGTCAAGTT CGCCTCCTCG 
0481 ATAACCGGAG CTCTGGACAA C

Assemble Data

<PRE>

11251R-1.IR_full       1   ACCTCCTTCGCAACTTCATTCCCAAGTGGTGTCTCCCACGTCCAGAGAAGCATCTCCAGA 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11251R-1.in silico     1   ACCTCCTTCGCAACTTCATTCCCAAGTGGTGTCTCCCACGTCCAGAGAAGCATCTCCAGA 60


11251R-1.IR_full       61  ACCACATATTCCGTCAGCTCCAGACGAATCACCCATCCGCCGCACTGGTGGCCATCAAAA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11251R-1.in silico     61  ACCACATATTCCGTCAGCTCCAGACGAATCACCCATCCGCCGCACTGGTGGCCATCAAAA 120


11251R-1.IR_full       121 CAAGTGAACAGGTGCTGCCCAAGTGCAGATTCATGCAGTACCGGCGCGCCAATGAGCAGG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11251R-1.in silico     121 CAAGTGAACAGGTGCTGCCCAAGTGCAGATTCATGCAGTACCGGCGCGCCAATGAGCAGG 180


11251R-1.IR_full       181 TGAAGCGATTGGGCATGGTCTCCGAGGATGGCAGCAAGATGGCGGAGCTA<span class="snp"><tt>N</tt></span>TCGAGCATG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||
11251R-1.in silico     181 TGAAGCGATTGGGCATGGTCTCCGAGGATGGCAGCAAGATGGCGGAGCTA<span class="snp"><tt>-</tt></span>TCGAGCATG 240


11251R-1.IR_full       241 ACCTGCGCCGCCCCCAATATGATGGACTTCATCCAGCAGAGATACTGCATGGTTAGCCTG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11251R-1.in silico     241 ACCTGCGCCGCCCCCAATATGATGGACTTCATCCAGCAGAGATACTGCATGGTTAGCCTG 300


11251R-1.IR_full       301 CTGGACAGTGTGCAATTCATGAAGATCGAGGATGTGGAGGCCGTGGATCTCCGCCTGCTG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11251R-1.in silico     301 CTGGACAGTGTGCAATTCATGAAGATCGAGGATGTGGAGGCCGTGGATCTCCGCCTGCTG 360


11251R-1.IR_full       361 CCACCCATCGATTCCCCC<span class="snp"><tt>G</tt></span>GTAAGATCATTGGCGTGGACTGCAACTATGTGGA<span class="snp"><tt>C</tt></span>AACTGT 420
                           ||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||
11251R-1.in silico     361 CCACCCATCGATTCCCCC<span class="snp"><tt>A</tt></span>GTAAGATCATTGGCGTGGACTGCAACTATGTGGA<span class="snp"><tt>T</tt></span>AACTGT 420


11251R-1.IR_full       421 GATGAGCAGCACATCTC<span class="snp"><tt>C</tt></span>ATTCCGAGGGAGCCGAG<span class="snp"><tt>C</tt></span>TTTCACGTCAAGTTCGCCTCCTCG 480
                           |||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||
11251R-1.in silico     421 GATGAGCAGCACATCTC<span class="snp"><tt>G</tt></span>ATTCCGAGGGAGCCGAG<span class="snp"><tt>T</tt></span>TTTCACGTCAAGTTCGCCTCCTCG 480


11251R-1.IR_full       481 ATAACCGGAGCTCTGGACAAC 501
                           |||||||||||||||||||||
11251R-1.in silico     481 ATAACCGGAGCTCTGGACAAC 501

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_140375.1	CG11251-RA (CG11251), mRNA
0	0	3	2	4	NM_132004.2	CG4136-RA (CG4136), mRNA
0	0	3	2	3	NM_137803.1	CG6758-RA (CG6758), mRNA
0	0	2	3	4	NM_132300.2	CG12664-RB (ld14), mRNA
0	0	2	3	3	NM_168843.1	CG32225-RA (CG32225), mRNA
0	0	1	5	2	NM_135485.4	CG4619-RA (CG4619), mRNA
0	0	1	3	8	NM_165090.1	CG31835-RA (CG31835), mRNA
0	0	1	3	3	NM_001043247.1	CG6535-RB (tefu), mRNA
0	0	1	3	2	NM_137578.2	CG7744-RA (CG7744), mRNA
0	0	1	2	4	NM_141026.1	CG10566-RA (CG10566), mRNA
0	0	1	2	3	NM_137925.2	CG3162-RA (CG3162), mRNA
0	0	1	2	3	NM_135643.2	CG6192-RA (CG6192), mRNA
0	0	1	2	2	NM_134512.4	CG12701-RA, transcript variant A (CG12701), mRNA
0	0	1	2	2	NM_167685.1	CG12701-RB, transcript variant B (CG12701), mRNA
0	0	1	2	2	NM_136788.1	CG12944-RA, transcript variant A (Obp47a), mRNA
0	0	1	2	2	NM_206088.1	CG12944-RB, transcript variant B (Obp47a), mRNA
0	0	1	2	2	NM_136224.2	CG14400-RA (CG14400), mRNA
0	0	0	8	4	NM_170513.1	CG31021-RA (CG31021), mRNA
0	0	0	6	18	NM_132335.2	CG12139-RB (CG12139), mRNA
0	0	0	6	3	NM_166290.1	CG30120-RA (CG30120), mRNA
0	0	0	5	16	NM_057368.3	CG2621-RD, transcript variant D (sgg), mRNA
0	0	0	5	5	NM_001031910.1	CG11566-RA (CG11566), mRNA
0	0	0	5	5	NM_001031911.1	CG33670-RA (CG33670), mRNA
0	0	0	5	4	NM_168844.1	CG6680-RB, transcript variant B (CG6680), mRNA
0	0	0	5	4	NM_140948.2	CG6680-RA, transcript variant A (CG6680), mRNA
0	0	0	5	4	NM_168232.2	CG32369-RA, transcript variant A (CG32369), mRNA
0	0	0	5	4	NM_139899.3	CG32369-RB, transcript variant B (CG32369), mRNA
0	0	0	4	10	NM_136158.2	CG10538-RA (CdGAPr), mRNA
0	0	0	4	5	NM_057420.3	CG7875-RA (trp), mRNA
0	0	0	4	5	NM_170512.1	CG31019-RA (CG31019), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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