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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
1114R-2 |
| Symbol |
Hph |
Full Name |
HIF prolyl hydroxylase |
| CG No |
CG31543 |
Old CG No |
CG1114 |
| Synonyms |
hph, CG31543, fga, CG14665, l(3)02255, CG1114, dmHPH, Dm.egl-9, 0303/04, anon-EST:Posey69, l(3)S030304, Hph |
| Accession No (Link to NCBI) |
NM_141268.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Dorogova NV, Khruscheva AS, Galimova IA, Oshchepkov DY, Maslov DE, Shvedkina ED, Akhmetova KA, Fedorova SA.<br> Migration of primordial germline cells is negatively regulated by surrounding somatic cells during early embryogenesis in Drosophila melanogaster.<br> Vavilovskii Zhurnal Genet Selektsii (2020) 24(5) 525-532
[
PubMed ID = 33659837
]
[
RRC reference
]
Álvarez-Fernández C, Tamirisa S, Prada F, Chernomoretz A, Podhajcer O, Blanco E, Martín-Blanco E.<br> Identification and functional analysis of healing regulators in Drosophila.<br> PLoS Genet (2015) 11(2) e1004965
[
PubMed ID = 25647511
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCACGACCACGGACTACAAAAA |
| Primer Seq. 3' |
aatctagaggtaccCCACATGCATGACGTAGTGAG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CACGACCACG GACTACAAAA ACTTTTTTNA AACACTCGGC ACATCCTGCC AATGCTGAGC
0061 AGTACTTTCG GGAACTTTTG GACAAGCGTG aGNGCCGCTA TGAGGATCTG TGCCGTAACA
0121 TCATCAGCGA CATGAACCAG TATGGTCTGT CCGTGGTGGA CGACTTCCTG GGGATGGAGA
0181 CGGGCCTGAA GATCCTCAAC GAGGTTCGAA GCATGTACAA CGCAGGAGCC TTCCAGGATG
0241 GCCAAGTGGT GACCAACCAG ACGCCCGANT GCACCCGCGG TGCGCGGTGN ACAAGATCCG
0301 AGGCGATAAG ATCAAGTGGG TTGGTGGCAA TGAGCCGGGC NTGCAGCAAT GTCTGGTATC
0361 TGACCAATCA GATTGACTCT GTGGTGTATC GTGTTAACAC GATGAAGGAT AATGGCATCT
0421 TGGGCAACTA CCACATCAGG GAGCGCACGA GGGCAATGGT CGCTTGTTAT CCGGGATCGG
0481 GAACTCACTA CGTCATGCAT GTGG |
| in silico PCR Fragment |
0001 CACGACCACG GACTACAAAA ACTTTTTT-A AACACTCGGC ACATCCTGCC AATGCTGAGC
0061 AGTACTTTCG GGAACTTTTG GACAAGCGTG AGCGCCGCTA TGAGGATCTG TGCCGTAACA
0121 TCATCAGCGA CATGAACCAG TATGGTCTGT CCGTGGTGGA CGACTTCCTG GGGATGGAGA
0181 CGGGCCTGAA GATCCTCAAC GAGGTTCGAA GCATGTACAA CGCAGGAGCC TTCCAGGATG
0241 GCCAAGTGGT GACCAACCAG ACGCCCGA-T GCACCCGCGG TGCGCGGTG- ACAAGATCCG
0301 AGGCGATAAG ATCAAGTGGG TTGGTGGCAA TGAGCCGGGC -TGCAGCAAT GTCTGGTATC
0361 TGACCAATCA GATTGACTCT GTGGTGTATC GTGTTAACAC GATGAAGGAT AATGGCATCT
0421 TGGGCAACTA CCACATCAGG GAGCGCACGA GGGCAATGGT CGCTTGTTAT CCGGGATCGG
0481 GAACTCACTA CGTCATGCAT GTGG |
| Assemble Data |
<PRE>
1114R-2.IR_full 1 CACGACCACGGACTACAAAAACTTTTTT<span class="snp"><tt>N</tt></span>AAACACTCGGCACATCCTGCCAATGCTGAGC 60
||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||
1114R-2.in silico 1 CACGACCACGGACTACAAAAACTTTTTT<span class="snp"><tt>-</tt></span>AAACACTCGGCACATCCTGCCAATGCTGAGC 60
1114R-2.IR_full 61 AGTACTTTCGGGAACTTTTGGACAAGCGTGAG<span class="snp"><tt>N</tt></span>GCCGCTATGAGGATCTGTGCCGTAACA 120
||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||
1114R-2.in silico 61 AGTACTTTCGGGAACTTTTGGACAAGCGTGAG<span class="snp"><tt>C</tt></span>GCCGCTATGAGGATCTGTGCCGTAACA 120
1114R-2.IR_full 121 TCATCAGCGACATGAACCAGTATGGTCTGTCCGTGGTGGACGACTTCCTGGGGATGGAGA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1114R-2.in silico 121 TCATCAGCGACATGAACCAGTATGGTCTGTCCGTGGTGGACGACTTCCTGGGGATGGAGA 180
1114R-2.IR_full 181 CGGGCCTGAAGATCCTCAACGAGGTTCGAAGCATGTACAACGCAGGAGCCTTCCAGGATG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1114R-2.in silico 181 CGGGCCTGAAGATCCTCAACGAGGTTCGAAGCATGTACAACGCAGGAGCCTTCCAGGATG 240
1114R-2.IR_full 241 GCCAAGTGGTGACCAACCAGACGCCCGA<span class="snp"><tt>N</tt></span>TGCACCCGCGGTGCGCGGTG<span class="snp"><tt>N</tt></span>ACAAGATCCG 300
||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||
1114R-2.in silico 241 GCCAAGTGGTGACCAACCAGACGCCCGA<span class="snp"><tt>-</tt></span>TGCACCCGCGGTGCGCGGTG<span class="snp"><tt>-</tt></span>ACAAGATCCG 300
1114R-2.IR_full 301 AGGCGATAAGATCAAGTGGGTTGGTGGCAATGAGCCGGGC<span class="snp"><tt>N</tt></span>TGCAGCAATGTCTGGTATC 360
||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||
1114R-2.in silico 301 AGGCGATAAGATCAAGTGGGTTGGTGGCAATGAGCCGGGC<span class="snp"><tt>-</tt></span>TGCAGCAATGTCTGGTATC 360
1114R-2.IR_full 361 TGACCAATCAGATTGACTCTGTGGTGTATCGTGTTAACACGATGAAGGATAATGGCATCT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1114R-2.in silico 361 TGACCAATCAGATTGACTCTGTGGTGTATCGTGTTAACACGATGAAGGATAATGGCATCT 420
1114R-2.IR_full 421 TGGGCAACTACCACATCAGGGAGCGCACGAGGGCAATGGTCGCTTGTTATCCGGGATCGG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1114R-2.in silico 421 TGGGCAACTACCACATCAGGGAGCGCACGAGGGCAATGGTCGCTTGTTATCCGGGATCGG 480
1114R-2.IR_full 481 GAACTCACTACGTCATGCATGTGG 504
||||||||||||||||||||||||
1114R-2.in silico 481 GAACTCACTACGTCATGCATGTGG 504
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
2 |
4 |
NM_141268.2 |
CG31543-RA, transcript variant A (Hph), mRNA |
| 82.15 |
396 |
1 |
3 |
5 |
NM_169036.1 |
CG31543-RC, transcript variant C (Hph), mRNA |
| 81.95 |
395 |
1 |
4 |
5 |
NM_169037.1 |
CG31543-RB, transcript variant B (Hph), mRNA |
| 0 |
0 |
2 |
5 |
6 |
NM_165341.1 |
CG1768-RB, transcript variant B (dia), mRNA |
| 0 |
0 |
2 |
5 |
6 |
NM_057633.3 |
CG1768-RA, transcript variant A (dia), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_132797.2 |
CG9198-RA, transcript variant A (shtd), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_167443.1 |
CG9198-RB, transcript variant B (shtd), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_206394.1 |
CG7555-RD, transcript variant D (Nedd4), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_168737.1 |
CG7555-RB, transcript variant B (Nedd4), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_168736.1 |
CG7555-RC, transcript variant C (Nedd4), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_140736.2 |
CG7555-RA, transcript variant A (Nedd4), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_139622.1 |
CG15011-RA (CG15011), mRNA |
| 0 |
0 |
0 |
7 |
7 |
NM_170512.1 |
CG31019-RA (CG31019), mRNA |
| 0 |
0 |
0 |
5 |
11 |
NM_135190.3 |
CG9539-RA (Sec61alpha), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_206236.1 |
CG15804-RB, transcript variant B (Dhc62B), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_057737.2 |
CG15804-RA, transcript variant A (Dhc62B), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_135247.2 |
CG9138-RA (SP1070), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_139742.1 |
CG13288-RA (CG13288), mRNA |
| 0 |
0 |
0 |
4 |
12 |
NM_001032051.1 |
CG33715-RD, transcript variant D (Msp-300), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_135839.2 |
CG7953-RA (CG7953), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_139421.3 |
CG5687-RA (CG5687), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_143209.1 |
CG6142-RA (CG6142), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_141529.2 |
CG11694-RA (CG11694), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_134745.1 |
CG14343-RA (CG14343), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_132701.2 |
CG11092-RA (CG11092), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_001032053.1 |
CG33715-RB, transcript variant B (Msp-300), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_132740.1 |
CG15890-RA (CG15890), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_057676.3 |
CG11312-RA (insc), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_165239.2 |
CG5758-RB, transcript variant B (CG5758), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_136022.5 |
CG5758-RA, transcript variant A (CG5758), mRNA |
|
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Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
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