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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 11136R-2 
 Symbol CG11136  Full Name CG11136 
 CG No CG11136  Old CG No CG11136 
 Synonyms tartan/capricious-like, CT31131, CG11136 
 Accession No (Link to NCBI) NM_137662.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS ONE (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgCTCGAGTGCGGATGCGATCTG 
 Primer Seq. 3'
aatctagaggtaccTCACCCAATCGATTCCCGCCC 
 Predicted Fragment Size
500 
 IR fragment full Seq
0001 CTCGAGTGCG GATGCGATGC TGCCTCACAC ACTTCGATGC AACATTGATC TGCACGGGAT 
0061 GATTGCTCCT GGCGGATCGA CTACGCACTT CTCCCTACTC CATATCATTG CTGGACTGTT 
0121 CCCTGCGCAA CGTGACCTTC CTAAAGCGAC GCGAAGATCT TCGACAATGT CTCGCTCCAC 
0181 GGACTAGTAA TCTCGTCCGG AGAAATCAAG CGGGTACACA AGTCCGCCGT TCCTGGGCAT 
0241 TCGAGGACCG CTGCAGGCAC TCGGTCTGCC GGGAAACGCA CTGATGAGCG TCCCCTGGAA 
0301 CGCCCTGTCC ACGCTGAGCG CCTNTGGAGC GCTTGGATCT GGCCAATAAC AAGATCAAGG 
0361 CTCTGGGAAC GGCGGACTTT GTGGGATTGA CTAGCTTGGT TTATCTGGAG CTGAGCAACA 
0421 ATCAAATCTC TAGCATATCC CAACGCACGT TTGTTAATCT TCGAAAGCTG GAGGTGCTGA 
0481 AGCTGGGCGG GAATCGATTG GGTGA 
 in silico PCR Fragment
0001 CTCGAGTGCG GATGCGAT-C TGCCTCACAC ACTTCGATGC AACATTGATC TGCACGGGAT 
0061 GA-TGCTCCT GGCGGATCGA CTACGCACTT CTCCCTACTC CATATCATTG CTGGACTGTT 
0121 CCTTGCGCAA CGTGACCTTC CT-GAGCGAC GCGAAGATCT TCGACAATGT CTCGCTCCAC 
0181 GGACTAGTAA TCTCGTCCGG AGAGATCAAG CGGGTACACA AGTCCGCC-T TCCTGGGCAT 
0241 TCGAGGACCG CTGCAAGCAC TCGGTCTGCC CGGGAACGCA CTGATGAGCG TGCCCTGGAA 
0301 CGCCCTGTCC ACGCTGAGCG CCT-TGGAGC GCTTGGATCT GGCCAATAAC AAGATCAAGG 
0361 CTCTGGGAAC GGCGGACTTT GTGGGATTGA CTAGCTTGGT TTATCTGGAG CTGAGCAACA 
0421 ATCAAATCTC TAGCATATCC CAACGCACGT TTGTTAATCT TCGAAAGCTG GAGGTGCTGA 
0481 AGCTGGGCGG GAATCGATTG GGTGA 
 Assemble Data

11136R-2.IR_full       1   CTCGAGTGCGGATGCGATGCTGCCTCACACACTTCGATGCAACATTGATCTGCACGGGAT 60
                           |||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||
11136R-2.in silico     1   CTCGAGTGCGGATGCGAT-CTGCCTCACACACTTCGATGCAACATTGATCTGCACGGGAT 60


11136R-2.IR_full       61  GATTGCTCCTGGCGGATCGACTACGCACTTCTCCCTACTCCATATCATTGCTGGACTGTT 120
                           || |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11136R-2.in silico     61  GA-TGCTCCTGGCGGATCGACTACGCACTTCTCCCTACTCCATATCATTGCTGGACTGTT 120


11136R-2.IR_full       121 CCCTGCGCAACGTGACCTTCCTAAAGCGACGCGAAGATCTTCGACAATGTCTCGCTCCAC 180
                           || |||||||||||||||||||  ||||||||||||||||||||||||||||||||||||
11136R-2.in silico     121 CCTTGCGCAACGTGACCTTCCT-GAGCGACGCGAAGATCTTCGACAATGTCTCGCTCCAC 180


11136R-2.IR_full       181 GGACTAGTAATCTCGTCCGGAGAAATCAAGCGGGTACACAAGTCCGCCGTTCCTGGGCAT 240
                           ||||||||||||||||||||||| |||||||||||||||||||||||| |||||||||||
11136R-2.in silico     181 GGACTAGTAATCTCGTCCGGAGAGATCAAGCGGGTACACAAGTCCGCC-TTCCTGGGCAT 240


11136R-2.IR_full       241 TCGAGGACCGCTGCAGGCACTCGGTCTGCCGGGAAACGCACTGATGAGCGTCCCCTGGAA 300
                           ||||||||||||||| |||||||||||||| || ||||||||||||||||| ||||||||
11136R-2.in silico     241 TCGAGGACCGCTGCAAGCACTCGGTCTGCCCGGGAACGCACTGATGAGCGTGCCCTGGAA 300


11136R-2.IR_full       301 CGCCCTGTCCACGCTGAGCGCCTNTGGAGCGCTTGGATCTGGCCAATAACAAGATCAAGG 360
                           ||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||
11136R-2.in silico     301 CGCCCTGTCCACGCTGAGCGCCT-TGGAGCGCTTGGATCTGGCCAATAACAAGATCAAGG 360


11136R-2.IR_full       361 CTCTGGGAACGGCGGACTTTGTGGGATTGACTAGCTTGGTTTATCTGGAGCTGAGCAACA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11136R-2.in silico     361 CTCTGGGAACGGCGGACTTTGTGGGATTGACTAGCTTGGTTTATCTGGAGCTGAGCAACA 420


11136R-2.IR_full       421 ATCAAATCTCTAGCATATCCCAACGCACGTTTGTTAATCTTCGAAAGCTGGAGGTGCTGA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11136R-2.in silico     421 ATCAAATCTCTAGCATATCCCAACGCACGTTTGTTAATCTTCGAAAGCTGGAGGTGCTGA 480


11136R-2.IR_full       481 AGCTGGGCGGGAATCGATTGGGTGA 505
                           |||||||||||||||||||||||||
11136R-2.in silico     481 AGCTGGGCGGGAATCGATTGGGTGA 505

 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  NM_137662.1  CG11136-RA (CG11136), mRNA 
0   NM_138093.2  CG4681-RA (CG4681), mRNA 
0   NM_130481.2  CG13375-RA (CG13375), mRNA 
0   NM_135264.1  CG4969-RA (Wnt6), mRNA 
0   NM_135340.2  CG8668-RA (CG8668), mRNA 
0   NM_137628.1  CG13868-RA (CG13868), mRNA 
0   NM_168811.1  CG32209-RB (CG32209), mRNA 
0   NM_057737.2  CG15804-RA, transcript variant A (Dhc62B), mRNA 
0   NM_206236.1  CG15804-RB, transcript variant B (Dhc62B), mRNA 
0   NM_136472.3  CG1399-RB, transcript variant B (CG1399), mRNA 
0   NM_142046.1  CG9591-RA (omd), mRNA 
0   NM_166546.2  CG30092-RD, transcript variant D (jbug), mRNA 
0   NM_141545.2  CG11732-RA (Obp85a), mRNA 
0   NM_058125.3  CG1341-RA (Rpt1), mRNA 
0   NM_170222.1  CG10618-RA (CHKov1), mRNA 
0   NM_169512.1  CG9813-RF, transcript variant F (CG9813), mRNA 
0   NM_169516.1  CG9813-RE, transcript variant E (CG9813), mRNA 
0   NM_169511.1  CG9813-RC, transcript variant C (CG9813), mRNA 
0   NM_169515.1  CG9813-RD, transcript variant D (CG9813), mRNA 
0   NM_169514.1  CG9813-RB, transcript variant B (CG9813), mRNA 
0   NM_169513.1  CG9813-RA, transcript variant A (CG9813), mRNA 
0   NM_206101.1  CG13168-RC, transcript variant C (CG13168), mRNA 
0   NM_206100.1  CG13168-RB, transcript variant B (CG13168), mRNA 
0   NM_132722.2  CG1434-RA (CG1434), mRNA 
0   NM_142838.3  CG31152-RA (CG31152), mRNA 
0   20  NM_143497.2  CG7896-RA (CG7896), mRNA 
0   10  NM_165089.2  CG3479-RA, transcript variant A (osp), mRNA 
0   10  NM_078843.4  CG3479-RB, transcript variant B (osp), mRNA 
0   NM_079331.2  CG11280-RA (trn), mRNA 
0   NM_132035.1  CG4064-RA (CG4064), mRNA 
 Wing Disc
GFP
Puc
Caspase
DAPI
Merge
Figure
JNK signal
N
DB
DC
DM
VM
+- 
VB
 
Caspase-3 signal
N
DB
++ 
DC
DM
VM
VB
 
Dcompartment   Penetrance
     

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