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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
11133R-4 |
| Symbol |
CG11133 |
Full Name |
CG11133 |
| CG No |
CG11133 |
Old CG No |
CG11133 |
| Synonyms |
CG11133 |
| Accession No (Link to NCBI) |
NM_141173.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Ohhara Y, Hoshino G, Imahori K, Matsuyuki T, Yamakawa-Kobayashi K.<br> The Nutrient-Responsive Molecular Chaperone Hsp90 Supports Growth and Development in <i>Drosophila</i>.<br> Front Physiol (2021) 12 690564
[
PubMed ID = 34239451
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGAAAAGACCTGGCAGAGCAGC |
| Primer Seq. 3' |
aatctagaggtaccTTCTTATGCGCCTTGCGCAGC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GAAAAGACCT GGCAGAGCAG CAGAGACTCA GTCTACGAAG TTGGCGACTC GTATAGCGGC
0061 CAGAATGCCA ATCGACCTCT GGTGGCCACG AACTGCTCCG AATATGCGGT GGCCCATGCA
0121 AACTGCCAGT TGCGTCCGGT TCGTCGATTT GCGGAGGTTT CTCTTCTGCC GGACATCCTG
0181 GAAACCATGA GAAACTTGGG CCTAAACCGG TTGCTCCGAC TGCAAAGCTA CACATGGCCA
0241 CACTTGGCCG GAGGATCTGG GCACGGGGCG ATGATTGTGG GGTCTCCGGC CAGTGGTCGC
0301 ACTTTTGCTT ATATACCACC TGTTTGCCAT GCTGTTTCCA GAGCTCTAAT GGATTTCACA
0361 GGTCAGTGCG AGGATGTGGA GCACGATATC TCTCAACCGG ATCGCTATGG CCCCATAGCA
0421 TTGATCCTCG TACCGGATCT GCGACGCGTT CACCAGGTCA GCGCGATGTG TCTANGCCCT
0481 GCTGCGCAAG GCGCATAAGA A |
| in silico PCR Fragment |
0001 GAAAAGACCT GGCAGAGCAG CAGAGACTCA GTCTACGAAG TTGGCGACTC GTATAGCGGC
0061 CAGAATGCCA ATCGACCTCT GGTGGCCACG AACTGCTCCG AATATGCGGT GGCCCATGCA
0121 AACTGCCAGT TGCGTCCGGT TCGTCGATTT GCGGAGGTTT CTCTTCTGCC GGACATCCTG
0181 GAAACCATGA GAAACTTGGG CCTAAACCGG TTGCTCCGAC TGCAAAGCTA CACATGGCCA
0241 CACTTGGCCG GAGGATCTGG GCACGGGGCG ATGATTGTGG GGTCTCCGGC CAGTGGTCGC
0301 ACTTTTGCTT ATATACCACC TGTTTGCCAT GCTGTTTCCA GAGCTCTAAT GGATTTCACA
0361 GGTCAGTGCG AGGATGTGGA GCACGATATC TCTCAACCGG ATCGCTATGG CCCCATAGCA
0421 TTGATCCTCG TACCGGATCT GCGACGCGTT CACCAGGTCA GCGCGATGTG TCTA-GCCCT
0481 GCTGCGCAAG GCGCATAAGA A |
| Assemble Data |
<PRE>
11133R-4.IR_full 1 GAAAAGACCTGGCAGAGCAGCAGAGACTCAGTCTACGAAGTTGGCGACTCGTATAGCGGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11133R-4.in silico 1 GAAAAGACCTGGCAGAGCAGCAGAGACTCAGTCTACGAAGTTGGCGACTCGTATAGCGGC 60
11133R-4.IR_full 61 CAGAATGCCAATCGACCTCTGGTGGCCACGAACTGCTCCGAATATGCGGTGGCCCATGCA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11133R-4.in silico 61 CAGAATGCCAATCGACCTCTGGTGGCCACGAACTGCTCCGAATATGCGGTGGCCCATGCA 120
11133R-4.IR_full 121 AACTGCCAGTTGCGTCCGGTTCGTCGATTTGCGGAGGTTTCTCTTCTGCCGGACATCCTG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11133R-4.in silico 121 AACTGCCAGTTGCGTCCGGTTCGTCGATTTGCGGAGGTTTCTCTTCTGCCGGACATCCTG 180
11133R-4.IR_full 181 GAAACCATGAGAAACTTGGGCCTAAACCGGTTGCTCCGACTGCAAAGCTACACATGGCCA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11133R-4.in silico 181 GAAACCATGAGAAACTTGGGCCTAAACCGGTTGCTCCGACTGCAAAGCTACACATGGCCA 240
11133R-4.IR_full 241 CACTTGGCCGGAGGATCTGGGCACGGGGCGATGATTGTGGGGTCTCCGGCCAGTGGTCGC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11133R-4.in silico 241 CACTTGGCCGGAGGATCTGGGCACGGGGCGATGATTGTGGGGTCTCCGGCCAGTGGTCGC 300
11133R-4.IR_full 301 ACTTTTGCTTATATACCACCTGTTTGCCATGCTGTTTCCAGAGCTCTAATGGATTTCACA 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11133R-4.in silico 301 ACTTTTGCTTATATACCACCTGTTTGCCATGCTGTTTCCAGAGCTCTAATGGATTTCACA 360
11133R-4.IR_full 361 GGTCAGTGCGAGGATGTGGAGCACGATATCTCTCAACCGGATCGCTATGGCCCCATAGCA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11133R-4.in silico 361 GGTCAGTGCGAGGATGTGGAGCACGATATCTCTCAACCGGATCGCTATGGCCCCATAGCA 420
11133R-4.IR_full 421 TTGATCCTCGTACCGGATCTGCGACGCGTTCACCAGGTCAGCGCGATGTGTCTA<span class="snp"><tt>N</tt></span>GCCCT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||
11133R-4.in silico 421 TTGATCCTCGTACCGGATCTGCGACGCGTTCACCAGGTCAGCGCGATGTGTCTA<span class="snp"><tt>-</tt></span>GCCCT 480
11133R-4.IR_full 481 GCTGCGCAAGGCGCATAAGAA 501
|||||||||||||||||||||
11133R-4.in silico 481 GCTGCGCAAGGCGCATAAGAA 501
</PRE>
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_141173.1 |
CG11133-RA (CG11133), mRNA |
| 0 |
0 |
2 |
5 |
5 |
NM_140188.2 |
CG6210-RA, transcript variant A (srt), mRNA |
| 0 |
0 |
2 |
5 |
5 |
NM_168450.1 |
CG6210-RB, transcript variant B (srt), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_137571.1 |
CG9416-RA (CG9416), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_079867.2 |
CG12220-RA (mRpL32), mRNA |
| 0 |
0 |
2 |
2 |
6 |
NM_080014.2 |
CG3064-RB (futsch), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_058039.2 |
CG11325-RA, transcript variant A (GRHR), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_205917.1 |
CG11325-RC, transcript variant C (GRHR), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_164705.1 |
CG11325-RB, transcript variant B (GRHR), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_132370.1 |
CG2989-RA (CG2989), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_169057.1 |
CG31547-RB, transcript variant B (CG31547), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_169058.1 |
CG31547-RA, transcript variant A (CG31547), mRNA |
| 0 |
0 |
1 |
3 |
1 |
NM_058049.4 |
CG6759-RA (cdc16), mRNA |
| 0 |
0 |
1 |
2 |
10 |
NM_167368.1 |
CG1640-RE, transcript variant E (CG1640), mRNA |
| 0 |
0 |
1 |
2 |
10 |
NM_167369.1 |
CG1640-RF, transcript variant F (CG1640), mRNA |
| 0 |
0 |
1 |
2 |
10 |
NM_167367.1 |
CG1640-RD, transcript variant D (CG1640), mRNA |
| 0 |
0 |
1 |
2 |
10 |
NM_132651.2 |
CG1640-RA, transcript variant A (CG1640), mRNA |
| 0 |
0 |
1 |
2 |
10 |
NM_167366.1 |
CG1640-RC, transcript variant C (CG1640), mRNA |
| 0 |
0 |
1 |
2 |
10 |
NM_167365.1 |
CG1640-RB, transcript variant B (CG1640), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_132210.2 |
CG2257-RB, transcript variant B (Ubc-E2H), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_167145.1 |
CG2257-RA, transcript variant A (Ubc-E2H), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_167146.1 |
CG2257-RC, transcript variant C (Ubc-E2H), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_137977.2 |
CG11299-RA (CG11299), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_168366.2 |
CG32046-RB, transcript variant B (CG32046), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_168367.1 |
CG32046-RA, transcript variant A (CG32046), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_135937.2 |
CG4930-RA (CG4930), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_143679.3 |
CG1793-RA, transcript variant A (MED26), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_166791.1 |
CG1793-RB, transcript variant B (MED26), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_132543.1 |
CG10362-RA (CG10362), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_132097.1 |
CG3950-RA (CG3950), mRNA |
|
| Wing Disc |
GFP
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Puc
 |
Caspase
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DAPI
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Merge
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Figure
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JNK signal
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Caspase-3 signal
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Pathways (updated: 2024/04/26)
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| KEGG Pathway |
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