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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 11123R-3 
 Symbol CG11123  Full Name CG11123 
 CG No CG11123  Old CG No CG11123 
 Synonyms CG11123 
 Accession No (Link to NCBI) NM_136432.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgCCAAGAAGAAGGGCAACCGCT 
 Primer Seq. 3'
aatctagaggtaccGTGATCATCTGTGAAGTCCGC 
 Predicted Fragment Size
500 
 IR fragment full Seq
0001 CCAAGAAGAA GGGCAACCNC TTTATgCGGA ACGCCCAAGG GGTTCGCCAA GCAGGGGATA 
0061 TTCGGCCGGG GTACGCACAT CGACGACGAG CAGTTCAACT ACTTCATCAA CATTCTGGAT 
0121 GCTATGAAAG TGGGATTCGA GGACGTAGAG GAGCGAGTCA TCATGGCCAA CAATGTGTTC 
0181 GAGCAGACCC ACGATCAGGA GATCCATCTG GCCTCCAATC AGATCGTGTC CAAGGCTCTG 
0241 GAGTCGTTAG TGGGCTTCGT GGACGACGTG CAGTTGGAGC GCTTTTTTAG CAAGTTTGGC 
0301 GACAAcCTCC GGCCCATGTG CTCCGATCGG TTTGCCTCGC ACGTCCTGCA GAAAATGCTG 
0361 GAAATCGCCT TTTTGCGCGG AGTGGGAAAA TCTGCAGCCC AGGATACCAG CGATGcNGNT 
0421 AGCGCCAACA AAAAAGCTAA GCCGGATGCC GCTCAAGAGG AGGAGGAGTA CAACCTGCAG 
0481 GCGGACTTCA CAGATGATCA C 
 in silico PCR Fragment
0001 CCAAGAAGAA GGGCAACCGC TTTATGCGGA ACG-CCAAGG GGTTCGCCAA GCAGGGGATA 
0061 TTCGGCCGGG GTACGCACAT CGACGACGAG CAGTTCAACT ACTTCATCAA CATTCTGGAT 
0121 GCTATGAAAG TGGGATTCGA GGACGTAGAG GAGCGAGTCA TCATGGCCAA CAATGTGTTC 
0181 GAGCAGACCC ACGATCAGGA GATCCATCTG GCCTCCAATC AGATCGTGTC CAAGGCTCTG 
0241 GAGTCGTTAG TGGGCTTCGT GGACGACGTG CAGTTGGAGC GCTTTTTTAG CAAGTTTGGC 
0301 GACAACCTCC GGCCCATGTG CTCCGATCGG TTTGCCTCGC ACGTCCTGCA GAAAATGCTG 
0361 GAAATCGCCT TTTTGCGCGG AGTGGGAAAA TCTGCAGCCC AGGATACCAG CGATGCAGCT 
0421 AGCGCCAACA AAAAAGCTAA GCCGGATGCC GCTCAAGAGG AGGAGGAGTA CAACCTGCAG 
0481 GCGGACTTCA CAGATGATCA C 
 Assemble Data

11123R-3.IR_full       1   CCAAGAAGAAGGGCAACCNCTTTATGCGGAACGCCCAAGGGGTTCGCCAAGCAGGGGATA 60
                           |||||||||||||||||| |||||||||||||| ||||||||||||||||||||||||||
11123R-3.in silico     1   CCAAGAAGAAGGGCAACCGCTTTATGCGGAACG-CCAAGGGGTTCGCCAAGCAGGGGATA 60


11123R-3.IR_full       61  TTCGGCCGGGGTACGCACATCGACGACGAGCAGTTCAACTACTTCATCAACATTCTGGAT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11123R-3.in silico     61  TTCGGCCGGGGTACGCACATCGACGACGAGCAGTTCAACTACTTCATCAACATTCTGGAT 120


11123R-3.IR_full       121 GCTATGAAAGTGGGATTCGAGGACGTAGAGGAGCGAGTCATCATGGCCAACAATGTGTTC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11123R-3.in silico     121 GCTATGAAAGTGGGATTCGAGGACGTAGAGGAGCGAGTCATCATGGCCAACAATGTGTTC 180


11123R-3.IR_full       181 GAGCAGACCCACGATCAGGAGATCCATCTGGCCTCCAATCAGATCGTGTCCAAGGCTCTG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11123R-3.in silico     181 GAGCAGACCCACGATCAGGAGATCCATCTGGCCTCCAATCAGATCGTGTCCAAGGCTCTG 240


11123R-3.IR_full       241 GAGTCGTTAGTGGGCTTCGTGGACGACGTGCAGTTGGAGCGCTTTTTTAGCAAGTTTGGC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11123R-3.in silico     241 GAGTCGTTAGTGGGCTTCGTGGACGACGTGCAGTTGGAGCGCTTTTTTAGCAAGTTTGGC 300


11123R-3.IR_full       301 GACAACCTCCGGCCCATGTGCTCCGATCGGTTTGCCTCGCACGTCCTGCAGAAAATGCTG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11123R-3.in silico     301 GACAACCTCCGGCCCATGTGCTCCGATCGGTTTGCCTCGCACGTCCTGCAGAAAATGCTG 360


11123R-3.IR_full       361 GAAATCGCCTTTTTGCGCGGAGTGGGAAAATCTGCAGCCCAGGATACCAGCGATGCNGNT 420
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||||| | |
11123R-3.in silico     361 GAAATCGCCTTTTTGCGCGGAGTGGGAAAATCTGCAGCCCAGGATACCAGCGATGCAGCT 420


11123R-3.IR_full       421 AGCGCCAACAAAAAAGCTAAGCCGGATGCCGCTCAAGAGGAGGAGGAGTACAACCTGCAG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11123R-3.in silico     421 AGCGCCAACAAAAAAGCTAAGCCGGATGCCGCTCAAGAGGAGGAGGAGTACAACCTGCAG 480


11123R-3.IR_full       481 GCGGACTTCACAGATGATCAC 501
                           |||||||||||||||||||||
11123R-3.in silico     481 GCGGACTTCACAGATGATCAC 501

 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  NM_136432.1  CG11123-RA (CG11123), mRNA 
0.2   NM_176103.1  CG14764-RA (CG14764), mRNA 
0   NM_135222.2  CG11188-RA (CG11188), mRNA 
0   NM_137252.2  CG8445-RA, transcript variant A (CG8445), mRNA 
0   NM_176194.1  CG8445-RB, transcript variant B (CG8445), mRNA 
0   NM_001038789.1  CG34011-RA (CG34011), mRNA 
0   NM_139448.2  CG32300-RB (oxt), mRNA 
0   NM_138025.2  CG3121-RA (CG3121), mRNA 
0   NM_143526.1  CG9737-RA (CG9737), mRNA 
0   NM_140119.2  CG6685-RA (CG6685), mRNA 
0   NM_079393.2  CG4083-RA (Mo25), mRNA 
0   NM_079617.3  CG7855-RA (timeout), mRNA 
0   NM_140889.1  CG14101-RA (CG14101), mRNA 
0   NM_140029.2  CG4942-RA (CG4942), mRNA 
0   NM_206212.1  CG3318-RB, transcript variant B (Dat), mRNA 
0   NM_136985.2  CG13320-RA, transcript variant A (Sans), mRNA 
0   NM_176160.1  CG13320-RB, transcript variant B (Sans), mRNA 
0   NM_057796.2  CG10379-RA (mbc), mRNA 
0   NM_167891.2  CG32313-RA (CG32313), mRNA 
0   NM_166546.2  CG30092-RD, transcript variant D (jbug), mRNA 
0   NM_139450.2  CG5714-RA (ecd), mRNA 
0   NM_141568.2  CG9797-RA (CG9797), mRNA 
0   NM_001014610.1  CG33546-RB, transcript variant B (gfzf), mRNA 
0   NM_001014609.1  CG33546-RC, transcript variant C (gfzf), mRNA 
0   12  NM_001014571.1  CG33556-RA (form3), mRNA 
0   NM_132622.1  CG18646-RA (CG18646), mRNA 
0   NM_142992.1  CG6454-RB, transcript variant B (CG6454), mRNA 
0   NM_139356.1  CG3524-RA (v(2)k05816), mRNA 
0   NM_132186.1  CG10932-RA (CG10932), mRNA 
0   NM_001014461.1  CG9967-RB, transcript variant B (CG9967), mRNA 
 Wing Disc
GFP
Puc
Caspase
DAPI
Merge
Figure
JNK signal
N
 
DB
+- 
DC
DM
+- 
VM
 
VB
 
Caspase-3 signal
N
DB
++ 
DC
DM
 
VM
 
VB
 
Dcompartment   Penetrance
     

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