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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 10973R-1 
 Symbol CG10973  Full Name CG10973 
 CG No CG10973  Old CG No CG10973 
 Synonyms CG10973 
 Accession No (Link to NCBI) NM_140337.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees semi-lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgATGACAGTGGATGCTGCAGCG 
 Primer Seq. 3' 
aatctagaggtaccCATCTCTGACAGACTTCTCGA 
 Predicted Fragment Size 
511 
 IR fragment full Seq 
0001 ATGACAGTGG ATGCTGCAGC GGCCCTGAAA GCAGCTTTGG TGATCTTGAA CAGCGAAGAA 
0061 TCCAGCACGG ACNGACCAAA TCGAGAGTCT GGATGTCATA AGGAGCCACA TCGATGACAT 
0121 CGACAATGCC ATAACGCTGG TGAAGCTAGG AGGAACTGCA ACTCTACTCC GCTACATAAC 
0181 ACATTCGGAT AGCGAAGTGC GAGAATCGGC ACTCAATACT GTCGCTGAAG TGGCCCAGAA 
0241 TAATGTATTT TGTCAGAACG CCCTCATAAA CGATAAGTTT TTGCCTGCAC TTGCAAAGAA 
0301 CTTGAGCCAT TCGAATCCGA ATACCGTGCG GTGCTCCTTG TATGCGATAT CATCGCTGAT 
0361 AAGGAACTTT CAGCCTGGAT ACGACGAGTT TAAGCGCATA AAGGGGATCA GGTCCTTGAT 
0421 ACCCTGTCTG AAATCCACAA ATACAAATGT CTACGTGAAG ACTGCTTTTC TAATTGCATC 
0481 GTTAACTTCA ATCGAGAAGT CTGTCAGAGA Tg 
 in silico PCR Fragment 
0001 ATGACAGTGG ATGCTGCAGC GGCCCTGAAA GCAGCTTTGG TGATCTTGAA CAGCGAAGAA 
0061 TCCAGCACGG AC-GACCAAA TCGAGAGTCT GGATGTCATA AGGAGCCACA TCGATGACAT 
0121 CGACAATGCC ATAACGCTGG TGAAGCTAGG AGGAACTGCA ACTCTACTCC GCTACATAAC 
0181 ACATTCGGAT AGCGAAGTGC GAGAATCGGC ACTCAATACT GTCGCTGAAG TGGCCCAGAA 
0241 TAATGTATTT TGTCAGAACG CCCTCATAAA CGATAAGTTT TTGCCTGCAC TTGCAAAGAA 
0301 CTTGAGCCAT TCGAATCCGA ATACCGTGCG GTGCTCCTTG TATGCGATAT CATCGCTGAT 
0361 AAGGAACTTT CAGCCTGGAT ACGACGAGTT TAAGCGCATA AAGGGGATCA GGTCCTTGAT 
0421 ACCCTGTCTG AAATCCACAA ATACAAATGT CTACGTGAAG ACTGCTTTTC TAATTGCATC 
0481 GTTAACTTCA ATCGAGAAGT CTGTCAGAGA TG 
 Assemble Data 
<PRE>

10973R-1.IR_full       1   ATGACAGTGGATGCTGCAGCGGCCCTGAAAGCAGCTTTGGTGATCTTGAACAGCGAAGAA 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10973R-1.in silico     1   ATGACAGTGGATGCTGCAGCGGCCCTGAAAGCAGCTTTGGTGATCTTGAACAGCGAAGAA 60


10973R-1.IR_full       61  TCCAGCACGGAC<span class="snp"><tt>N</tt></span>GACCAAATCGAGAGTCTGGATGTCATAAGGAGCCACATCGATGACAT 120
                           ||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||
10973R-1.in silico     61  TCCAGCACGGAC<span class="snp"><tt>-</tt></span>GACCAAATCGAGAGTCTGGATGTCATAAGGAGCCACATCGATGACAT 120


10973R-1.IR_full       121 CGACAATGCCATAACGCTGGTGAAGCTAGGAGGAACTGCAACTCTACTCCGCTACATAAC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10973R-1.in silico     121 CGACAATGCCATAACGCTGGTGAAGCTAGGAGGAACTGCAACTCTACTCCGCTACATAAC 180


10973R-1.IR_full       181 ACATTCGGATAGCGAAGTGCGAGAATCGGCACTCAATACTGTCGCTGAAGTGGCCCAGAA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10973R-1.in silico     181 ACATTCGGATAGCGAAGTGCGAGAATCGGCACTCAATACTGTCGCTGAAGTGGCCCAGAA 240


10973R-1.IR_full       241 TAATGTATTTTGTCAGAACGCCCTCATAAACGATAAGTTTTTGCCTGCACTTGCAAAGAA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10973R-1.in silico     241 TAATGTATTTTGTCAGAACGCCCTCATAAACGATAAGTTTTTGCCTGCACTTGCAAAGAA 300


10973R-1.IR_full       301 CTTGAGCCATTCGAATCCGAATACCGTGCGGTGCTCCTTGTATGCGATATCATCGCTGAT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10973R-1.in silico     301 CTTGAGCCATTCGAATCCGAATACCGTGCGGTGCTCCTTGTATGCGATATCATCGCTGAT 360


10973R-1.IR_full       361 AAGGAACTTTCAGCCTGGATACGACGAGTTTAAGCGCATAAAGGGGATCAGGTCCTTGAT 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10973R-1.in silico     361 AAGGAACTTTCAGCCTGGATACGACGAGTTTAAGCGCATAAAGGGGATCAGGTCCTTGAT 420


10973R-1.IR_full       421 ACCCTGTCTGAAATCCACAAATACAAATGTCTACGTGAAGACTGCTTTTCTAATTGCATC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10973R-1.in silico     421 ACCCTGTCTGAAATCCACAAATACAAATGTCTACGTGAAGACTGCTTTTCTAATTGCATC 480


10973R-1.IR_full       481 GTTAACTTCAATCGAGAAGTCTGTCAGAGATG 512
                           ||||||||||||||||||||||||||||||||
10973R-1.in silico     481 GTTAACTTCAATCGAGAAGTCTGTCAGAGATG 512

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   493  0  0  0  NM_140337.2  CG10973-RA, transcript variant A (CG10973), mRNA 
100   493  0  0  0  NM_001038913.1  CG10973-RB, transcript variant B (CG10973), mRNA 
0   0  2  4  3  NM_137238.2  CG8403-RA (SP2353), mRNA 
0   0  2  4  3  NM_134573.1  CG12092-RA (NPC1b), mRNA 
0   0  2  3  6  NM_168367.1  CG32046-RA, transcript variant A (CG32046), mRNA 
0   0  2  3  6  NM_168366.2  CG32046-RB, transcript variant B (CG32046), mRNA 
0   0  2  3  2  NM_176104.1  CG33087-RC (CG33087), mRNA 
0   0  2  2  4  NM_166099.1  CG8182-RB, transcript variant B (GalNAc-T1), mRNA 
0   0  2  2  4  NM_137199.2  CG8182-RA, transcript variant A (GalNAc-T1), mRNA 
0   0  2  2  2  NM_136992.2  CG13325-RA (CG13325), mRNA 
0   0  2  2  2  NM_167691.1  CG32824-RA (CG32824), mRNA 
0   0  2  2  2  NM_167466.1  CG9012-RB, transcript variant B (Chc), mRNA 
0   0  2  2  2  NM_057694.2  CG9012-RA, transcript variant A (Chc), mRNA 
0   0  2  2  2  NM_206729.1  CG9012-RC, transcript variant C (Chc), mRNA 
0   0  2  2  2  NM_206728.1  CG9012-RD, transcript variant D (Chc), mRNA 
0   0  1  4  7  NM_143615.2  CG11518-RA (pygo), mRNA 
0   0  1  3  4  NM_001043208.1  CG41467-RA, transcript variant A (CG41467), mRNA 
0   0  1  3  4  NM_001043207.1  CG41467-RB, transcript variant B (CG41467), mRNA 
0   0  1  3  2  NM_164448.1  CG4244-RA, transcript variant A (Su(dx)), mRNA 
0   0  1  3  2  NM_057405.2  CG4244-RB, transcript variant B (Su(dx)), mRNA 
0   0  1  3  2  NM_164449.1  CG4244-RC, transcript variant C (Su(dx)), mRNA 
0   0  1  2  3  NM_137111.1  CG12505-RA (CG12505), mRNA 
0   0  1  2  3  NM_141953.1  CG18554-RA (CG18554), mRNA 
0   0  1  2  2  NM_165292.1  CG31798-RA (CG31798), mRNA 
0   0  0  5  8  NM_135031.3  CG12787-RA, transcript variant A (hoe1), mRNA 
0   0  0  5  5  NM_135032.1  CG12787-RC, transcript variant C (hoe1), mRNA 
0   0  0  5  5  NM_175967.1  CG12787-RE, transcript variant E (hoe1), mRNA 
0   0  0  5  5  NM_164603.1  CG12787-RB, transcript variant B (hoe1), mRNA 
0   0  0  5  5  NM_175968.1  CG12787-RF, transcript variant F (hoe1), mRNA 
0   0  0  5  5  NM_175966.1  CG12787-RD, transcript variant D (hoe1), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
+- 
DC
+- 
DM
 
VM
 
VB
 
Caspase-3 signal
N
+ 
DB
+ 
DC
+ 
DM
 
VM
 
VB
 
Dcompartment   Penetrance
     
 Pathways (updated: 2024/04/26)
 KEGG Pathway dme04141 : Protein processing in endoplasmic reticulum

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