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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
10913R-1 |
| Symbol |
Spn6 |
Full Name |
Serine protease inhibitor 6 |
| CG No |
CG10913 |
Old CG No |
CG10913 |
| Synonyms |
CG10913, sp6, sp-6, Spn6 |
| Accession No (Link to NCBI) |
NM_080214.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
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| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Gubb D, Sanz-Parra A, Barcena L, Troxler L, Fullaondo A.
Protease inhibitors and proteolytic signalling cascades in insects.
Biochimie (2010) 92(12) 1749-59
[
PubMed ID = 20850496
]
[
RRC reference
]
Nakaoka T, Iga M, Yamada T, Koujima I, Takeshima M, Zhou X, Suzuki Y, Ogihara MH, Kataoka H.
Deep sequencing of the prothoracic gland transcriptome reveals new players in insect ecdysteroidogenesis.
PLoS ONE (2017) 12(3) e0172951
[
PubMed ID = 28257485
]
[
RRC reference
]
Álvarez-Fernández C, Tamirisa S, Prada F, Chernomoretz A, Podhajcer O, Blanco E, Martín-Blanco E.
Identification and functional analysis of healing regulators in Drosophila.
PLoS Genet. (2015) 11(2) e1004965
[
PubMed ID = 25647511
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS ONE (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCAATTTGGAATTTGCGCGTGG |
| Primer Seq. 3' |
aatctagaggtaccCCTTGAAGTGGAGGGCATTGA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CAATTTGGAA TTTGCGCGTG GAGGTGCCCG TTTCACCAGC GAACTTTTTC AGCTTTTGAG
0061 CGCTGGCGGG CTCAAGGAGA ACGTCGTCTT CTCGCCCTTC TCCATCCAAA CTTGCATTGC
0121 CCTGGCCTTT GCCGGATCGC AGGGTGAAAC CGCCGACGAG ATCGCCAAGG CACTGCACTT
0181 CGTCTCCAAC TTTCCGCCGG AGGTGGCCCA AACGTTCCAG TTTGTACTGG AGAAGTACCG
0241 CAACAGTAAC CTGCTGCGCG TTGCCAACAA GCTGTACGTG CAAGAGGGCA AGCANGCTGA
0301 AGCCCGCCTA CCAGTCGGCT ATCAAGGAGC AGTATCATTC GGAGGCGGAG TCCATTAACT
0361 TTGCGCTCAA CGATGCCGCC GCCCAGGCCA TCAATGCCTG GGTGAATGCC AAGACTCAGG
0421 GCAAGATTAC GGAGCTGGTC AGCGCAGGAC TCGTTCTCGG ACAACACCAG GCTGGTCCTG
0481 CTCAATGCCC TCCACTTCAA GG |
| in silico PCR Fragment |
0001 CAATTTGGAA TTTGCGCGTG GAGGTGCCCG TTTCACCAGC GAACTTTTTC AGCTTTTGAG
0061 CGCTGGCGGG CTCAAGGAGA ACGTCGTCTT CTCGCCCTTC TCCATCCAAA CTTGCATTGC
0121 CCTGGCCTTT GCCGGATCGC AGGGTGAAAC CGCCGACGAG ATCGCCAAGG CACTGCACTT
0181 CGTCTCCAAC TTTCCGCCGG AGGTGGCCCA AACGTTCCAG TTTGTGCTGG AGAAGTACCG
0241 CAACAGTAAC CTGCTGCGCG TTGCCAACAA GCTGTACGTG CAGGAGGGCA AGCA-GCTGA
0301 AGCCCGCCTA CCAGTCGGCC ATCAAGGAGC AGTATCATTC GGAGGCGGAG TCCATTAACT
0361 TTGCGCTCAA CGATGCCGCC GCCCAGGCCA TCAATGCCTG GGTGAATGCC AAGACTCAGG
0421 GCAAGATTAC GGAGCTGGTC AGCGC-GGAC TCGTTCTCGG ACAACACCAG GCTGGTCCTG
0481 CTCAATGCCC TCCACTTCAA GG |
| Assemble Data |
10913R-1.IR_full 1 CAATTTGGAATTTGCGCGTGGAGGTGCCCGTTTCACCAGCGAACTTTTTCAGCTTTTGAG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10913R-1.in silico 1 CAATTTGGAATTTGCGCGTGGAGGTGCCCGTTTCACCAGCGAACTTTTTCAGCTTTTGAG 60
10913R-1.IR_full 61 CGCTGGCGGGCTCAAGGAGAACGTCGTCTTCTCGCCCTTCTCCATCCAAACTTGCATTGC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10913R-1.in silico 61 CGCTGGCGGGCTCAAGGAGAACGTCGTCTTCTCGCCCTTCTCCATCCAAACTTGCATTGC 120
10913R-1.IR_full 121 CCTGGCCTTTGCCGGATCGCAGGGTGAAACCGCCGACGAGATCGCCAAGGCACTGCACTT 180
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10913R-1.in silico 121 CCTGGCCTTTGCCGGATCGCAGGGTGAAACCGCCGACGAGATCGCCAAGGCACTGCACTT 180
10913R-1.IR_full 181 CGTCTCCAACTTTCCGCCGGAGGTGGCCCAAACGTTCCAGTTTGTACTGGAGAAGTACCG 240
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10913R-1.in silico 181 CGTCTCCAACTTTCCGCCGGAGGTGGCCCAAACGTTCCAGTTTGTGCTGGAGAAGTACCG 240
10913R-1.IR_full 241 CAACAGTAACCTGCTGCGCGTTGCCAACAAGCTGTACGTGCAAGAGGGCAAGCANGCTGA 300
|||||||||||||||||||||||||||||||||||||||||| ||||||||||| |||||
10913R-1.in silico 241 CAACAGTAACCTGCTGCGCGTTGCCAACAAGCTGTACGTGCAGGAGGGCAAGCA-GCTGA 300
10913R-1.IR_full 301 AGCCCGCCTACCAGTCGGCTATCAAGGAGCAGTATCATTCGGAGGCGGAGTCCATTAACT 360
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10913R-1.in silico 301 AGCCCGCCTACCAGTCGGCCATCAAGGAGCAGTATCATTCGGAGGCGGAGTCCATTAACT 360
10913R-1.IR_full 361 TTGCGCTCAACGATGCCGCCGCCCAGGCCATCAATGCCTGGGTGAATGCCAAGACTCAGG 420
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10913R-1.in silico 361 TTGCGCTCAACGATGCCGCCGCCCAGGCCATCAATGCCTGGGTGAATGCCAAGACTCAGG 420
10913R-1.IR_full 421 GCAAGATTACGGAGCTGGTCAGCGCAGGACTCGTTCTCGGACAACACCAGGCTGGTCCTG 480
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10913R-1.in silico 421 GCAAGATTACGGAGCTGGTCAGCGC-GGACTCGTTCTCGGACAACACCAGGCTGGTCCTG 480
10913R-1.IR_full 481 CTCAATGCCCTCCACTTCAAGG 502
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10913R-1.in silico 481 CTCAATGCCCTCCACTTCAAGG 502
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_080214.2 |
CG10913-RA (Spn6), mRNA |
| 0 |
0 |
12 |
9 |
4 |
NM_080216.2 |
CG9453-RB, transcript variant B (Spn4), mRNA |
| 0 |
0 |
12 |
9 |
4 |
NM_206032.1 |
CG9453-RK, transcript variant K (Spn4), mRNA |
| 0 |
0 |
12 |
9 |
4 |
NM_206035.1 |
CG9453-RH, transcript variant H (Spn4), mRNA |
| 0 |
0 |
12 |
9 |
4 |
NM_165496.2 |
CG9453-RA, transcript variant A (Spn4), mRNA |
| 0 |
0 |
12 |
9 |
4 |
NM_165495.2 |
CG9453-RC, transcript variant C (Spn4), mRNA |
| 0 |
0 |
12 |
9 |
4 |
NM_206033.1 |
CG9453-RJ, transcript variant J (Spn4), mRNA |
| 0 |
0 |
12 |
9 |
4 |
NM_206036.1 |
CG9453-RG, transcript variant G (Spn4), mRNA |
| 0 |
0 |
12 |
9 |
4 |
NM_206034.1 |
CG9453-RI, transcript variant I (Spn4), mRNA |
| 0 |
0 |
12 |
9 |
4 |
NM_206037.1 |
CG9453-RF, transcript variant F (Spn4), mRNA |
| 0 |
0 |
12 |
9 |
4 |
NM_206039.1 |
CG9453-RD, transcript variant D (Spn4), mRNA |
| 0 |
0 |
12 |
9 |
4 |
NM_206038.1 |
CG9453-RE, transcript variant E (Spn4), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_058045.4 |
CG9148-RB, transcript variant B (scf), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_206231.1 |
CG9148-RC, transcript variant C (scf), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_058044.4 |
CG9148-RA, transcript variant A (scf), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_001038784.1 |
CG8867-RB, transcript variant B (Jon25Bi), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_078753.2 |
CG8867-RA, transcript variant A (Jon25Bi), mRNA |
| 0 |
0 |
2 |
12 |
6 |
NM_080112.1 |
CG1857-RA (nec), mRNA |
| 0 |
0 |
2 |
6 |
4 |
NM_176501.1 |
CG18525-RB, transcript variant B (Spn5), mRNA |
| 0 |
0 |
2 |
6 |
4 |
NM_080215.2 |
CG18525-RA, transcript variant A (Spn5), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_141985.1 |
CG7381-RA, transcript variant A (CG7381), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_169485.1 |
CG7381-RB, transcript variant B (CG7381), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_169486.1 |
CG7381-RC, transcript variant C (CG7381), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_137077.2 |
CG13350-RA (CG13350), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_132483.2 |
CG1737-RA (CG1737), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_079738.2 |
CG6768-RA (DNApol-epsilon), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_079088.2 |
CG9952-RA (ppa), mRNA |
| 0 |
0 |
1 |
9 |
11 |
NM_080219.3 |
CG9456-RA (Spn1), mRNA |
| 0 |
0 |
1 |
5 |
5 |
NM_169629.1 |
CG6476-RB, transcript variant B (Su(var)3-9), mRNA |
| 0 |
0 |
1 |
5 |
5 |
NM_169630.1 |
CG6476-RC, transcript variant C (Su(var)3-9), mRNA |
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