NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

10907R-1

Symbol

CG10907

Full Name

CG10907

CG No

CG10907

Old CG No

CG10907

Synonyms

CG10907

Accession No (Link to NCBI)

NM_140251.2

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgAAGACCACTGTGGGCGACATA

Primer Seq. 3'

aatctagaggtaccGTGGAAGGTTGGGAGAGAATA

Predicted Fragment Size

IR fragment full Seq

0001 AAGACCACTG TGGGCGACAT AGACATCGAG TTGTGGGCAC GGGAGTGTCC GAAgGCGTGC 
0061 CGCAACTTTG TGCAACTCTG CCTAGAGGGC TACTACAAGA ACACCGAATT CCACCGGCTG 
0121 GTCAAGGGCT TTATTGTCCA GGGAGGCGAT CCGAATGGCG ACGGCACCGG CGGCGAGTCC 
0181 ATATACGGAC AGCCCTTCAA GGATGAGTTC CACTCCCGAC TCCGGTACAC GCGTCGTGGC 
0241 CTTGTGGGCA TGGCAAATTC TGGAAAGGAT GACAATGGCT CCCAGTTCTT CTTCACATTT 
0301 GCTCCGACAC CGGAGCTGCA GAATAAGAAC ACGCTTTTTG GAAAGATCAC CGGCGACACC 
0361 ATCTACAACA TGCTCAAGCT GGAGGATGGC ATTGTTGACC ACCAGGAAAG ACCGATGCAC 
0421 GCACATCGCA TCGTTTCTAC GGAAGTCCTC TCGAACCCAT TCGATGATAT CGTTCCTCGT 
0481 ATTCTCTCCC AACCTTCCAC

in silico PCR Fragment

0001 AAGACCACTG TGGGCGACAT AGACATCGAG TTGTGGGCAC GGGAGTGTCC GAAGGCGTGC 
0061 CGCAACTTTG TGCAACTCTG CCTAGAGGGC TACTACAAGA ACACCGAATT CCACCGGCTG 
0121 GTCAAGGGCT TTATTGTCCA GGGAGGCGAT CCGAATGGCG ACGGCACCGG CGGCGAGTCC 
0181 ATATACGGAC AGCCCTTCAA GGATGAGTTC CACTCCCGAC TCCGGTACAC GCGTCGTGGC 
0241 CTCGTCGGCA TGGCAAATTC TGGAAAGGAT GACAATGGCT CACAGTTCTT CTTCACATTT 
0301 GCTCCGACAC CGGAGCTGCA GAATAAGAAC ACGCTTTTTG GAAAGATCAC CGGCGATACC 
0361 ATCTACAACA TGCTCAAGCT GGAAGATGGC ATTGTTGACC ACCAGGAAAG ACCGATGCAC 
0421 GCACATCGCA TCGTTTCTAC GGAAGTCCTC TCGAACCCAT TCGATGATAT CGTTCCTCGT 
0481 ATTCTCTCCC AACCTTCCAC

Assemble Data

<PRE>

10907R-1.IR_full       1   AAGACCACTGTGGGCGACATAGACATCGAGTTGTGGGCACGGGAGTGTCCGAAGGCGTGC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10907R-1.in silico     1   AAGACCACTGTGGGCGACATAGACATCGAGTTGTGGGCACGGGAGTGTCCGAAGGCGTGC 60


10907R-1.IR_full       61  CGCAACTTTGTGCAACTCTGCCTAGAGGGCTACTACAAGAACACCGAATTCCACCGGCTG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10907R-1.in silico     61  CGCAACTTTGTGCAACTCTGCCTAGAGGGCTACTACAAGAACACCGAATTCCACCGGCTG 120


10907R-1.IR_full       121 GTCAAGGGCTTTATTGTCCAGGGAGGCGATCCGAATGGCGACGGCACCGGCGGCGAGTCC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10907R-1.in silico     121 GTCAAGGGCTTTATTGTCCAGGGAGGCGATCCGAATGGCGACGGCACCGGCGGCGAGTCC 180


10907R-1.IR_full       181 ATATACGGACAGCCCTTCAAGGATGAGTTCCACTCCCGACTCCGGTACACGCGTCGTGGC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10907R-1.in silico     181 ATATACGGACAGCCCTTCAAGGATGAGTTCCACTCCCGACTCCGGTACACGCGTCGTGGC 240


10907R-1.IR_full       241 CT<span class="snp"><tt>T</tt></span>GT<span class="snp"><tt>G</tt></span>GGCATGGCAAATTCTGGAAAGGATGACAATGGCTC<span class="snp"><tt>C</tt></span>CAGTTCTTCTTCACATTT 300
                           ||<span class="snp"><tt>&nbsp;</tt></span>||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||
10907R-1.in silico     241 CT<span class="snp"><tt>C</tt></span>GT<span class="snp"><tt>C</tt></span>GGCATGGCAAATTCTGGAAAGGATGACAATGGCTC<span class="snp"><tt>A</tt></span>CAGTTCTTCTTCACATTT 300


10907R-1.IR_full       301 GCTCCGACACCGGAGCTGCAGAATAAGAACACGCTTTTTGGAAAGATCACCGGCGA<span class="snp"><tt>C</tt></span>ACC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||
10907R-1.in silico     301 GCTCCGACACCGGAGCTGCAGAATAAGAACACGCTTTTTGGAAAGATCACCGGCGA<span class="snp"><tt>T</tt></span>ACC 360


10907R-1.IR_full       361 ATCTACAACATGCTCAAGCTGGA<span class="snp"><tt>G</tt></span>GATGGCATTGTTGACCACCAGGAAAGACCGATGCAC 420
                           |||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||
10907R-1.in silico     361 ATCTACAACATGCTCAAGCTGGA<span class="snp"><tt>A</tt></span>GATGGCATTGTTGACCACCAGGAAAGACCGATGCAC 420


10907R-1.IR_full       421 GCACATCGCATCGTTTCTACGGAAGTCCTCTCGAACCCATTCGATGATATCGTTCCTCGT 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10907R-1.in silico     421 GCACATCGCATCGTTTCTACGGAAGTCCTCTCGAACCCATTCGATGATATCGTTCCTCGT 480


10907R-1.IR_full       481 ATTCTCTCCCAACCTTCCAC 500
                           ||||||||||||||||||||
10907R-1.in silico     481 ATTCTCTCCCAACCTTCCAC 500

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_140251.2	CG10907-RA (CG10907), mRNA
0.82	4	12	7	21	NM_143361.3	CG1866-RB, transcript variant B (Moca-cyp), mRNA
0.82	4	12	7	16	NM_170367.1	CG1866-RA, transcript variant A (Moca-cyp), mRNA
0	0	4	3	4	NM_164780.1	CG7392-RC, transcript variant C (Cka), mRNA
0	0	4	3	4	NM_164781.1	CG7392-RD, transcript variant D (Cka), mRNA
0	0	4	3	4	NM_164779.1	CG7392-RB, transcript variant B (Cka), mRNA
0	0	4	3	4	NM_135333.2	CG7392-RA, transcript variant A (Cka), mRNA
0	0	3	2	2	NM_176406.1	CG12746-RD, transcript variant D (CG12746), mRNA
0	0	3	2	2	NM_169067.2	CG12746-RB, transcript variant B (CG12746), mRNA
0	0	3	2	2	NM_169065.1	CG12746-RA, transcript variant A (CG12746), mRNA
0	0	3	2	2	NM_141294.3	CG12746-RC, transcript variant C (CG12746), mRNA
0	0	2	5	5	NM_079049.2	CG4886-RA (cyp33), mRNA
0	0	2	4	5	NM_166539.1	CG2852-RB, transcript variant B (CG2852), mRNA
0	0	2	4	5	NM_137851.2	CG2852-RA, transcript variant A (CG2852), mRNA
0	0	1	3	2	NM_078509.2	CG3929-RA (dx), mRNA
0	0	1	3	2	NM_079885.1	CG1507-RB, transcript variant B (Pur-alpha), mRNA
0	0	1	3	2	NM_166767.1	CG1507-RA, transcript variant A (Pur-alpha), mRNA
0	0	1	3	2	NM_135336.2	CG7466-RA (CG7466), mRNA
0	0	1	2	4	NM_132323.1	CG17438-RA (CG17438), mRNA
0	0	1	2	3	NM_078537.2	CG10966-RA, transcript variant A (rdgA), mRNA
0	0	1	2	3	NM_138101.2	CG3579-RA (CG3579), mRNA
0	0	1	2	3	NM_001042799.1	CG10966-RB, transcript variant B (rdgA), mRNA
0	0	1	2	3	NM_168231.1	CG32371-RA (CG32371), mRNA
0	0	1	2	2	NM_165776.1	CG11777-RA (CG11777), mRNA
0	0	0	6	10	NM_078642.3	CG9916-RA (Cyp1), mRNA
0	0	0	5	11	NM_079741.2	CG10210-RA (tst), mRNA
0	0	0	5	4	NM_135684.1	CG16965-RA (CG16965), mRNA
0	0	0	5	2	NM_136620.2	CG8008-RA (CG8008), mRNA
0	0	0	4	6	NM_130658.2	CG10260-RB (CG10260), mRNA
0	0	0	4	5	NM_131938.1	CG3568-RA (CG3568), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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