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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 10901R-2

Symbol osk Full Name oskar

CG No CG10901 Old CG No CG10901

Synonyms CG10901, osk, Oskar, Osk, OSK

Accession No (Link to NCBI) NM_169248.2

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Cho Y, Lai CM, Lin KY, Hsu HJ.
A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.
G3 (Bethesda) (2018) 8(7) 2345-2354 [ PubMed ID = 29764959 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATTCCCCAGCAAACCGATCAG

Primer Seq. 3'

aatctagaggtaccTCACTCTTGATGCTCGATATC

Predicted Fragment Size

IR fragment full Seq

0001 aTTCCCCAGC AAACCGATCA GTTATACCAG CACCAATACT TCCGCCAAAA CCTATTATCT 
0061 TAAGTCCGTG AAAAAGCGGG TGACCACGTG TTTCCAGCAG TTGCGCNGAT AAACTCCAGT 
0121 CATCCGGTTC CTTTCGCAAG AGTTCCTCCA GCTGCCTCAA CCAGATCTTC GTAAGGTCCG 
0181 ATTTCTCCGC ATGCGGCGAA CGCTTTCGGA AGATCTTCAA ATCGGCGCGA AAAACCGAAT 
0241 TGCCGGAGTT GTGGAAGGTG CCATTGGTGG CCCATGAACT CACCAGCCGG CAGAGCAGCC 
0301 AGCAGTTGCA AGTCGTAGCC AGACTCTTCT CGTCCACTCA GATTTCCACC AAGGAAATCA 
0361 CCTACAACAG CAACAGCAAC ACCAGCGAGA ACAACATGAC CATCATCGAG AGCAACTATA 
0421 TATCCGTGCG CGAGGAATAT CCCGATATAG ATAGTGAGGT GCGCGCCATA TTGCTGAgCC 
0481 ACGCCCAGAA TGGAATCACG ATATCGAGCA TCAAGAGTGA

in silico PCR Fragment

0001 ATTCCCCAGC AAACCGATCA GTTATACCAG CACCAATACT TCCGCCAAAA CCTATTATCT 
0061 TAAGTCCGTG AAAAAGCGGG TGACCACGTG TTTCCAGCAG TTGCGC-GAT AAACTCCAGT 
0121 CATCCGGTTC CTTTCGCAAG AGTTCCTCCA GCTGCCTCAA CCAGATCTTC GTAAGGTCCG 
0181 ATTTCTCCGC ATGCGGCGAA CGCTTTCGGA AGATCTTCAA ATCGGCGCGA AAAACCGAAT 
0241 TGCCGGAGTT GTGGAAGGTG CCATTGGTGG CCCATGAACT CACCAGCCGG CAGAGCAGCC 
0301 AGCAGTTGCA AGTCGTAGCC AGACTCTTCT CGTCCACTCA GATTTCCACC AAGGAAATCA 
0361 CCTACAACAG CAACAGCAAC ACCAGCGAGA ACAACATGAC CATCATCGAG AGCAACTATA 
0421 TATCCGTGCG CGAGGAATAT CCCGATATAG ATAGTGAGGT GCGCGCCATA TTGCTGAGCC 
0481 ACGCCCAGAA TGGAATCACG ATATCGAGCA TCAAGAGTGA

Assemble Data


10901R-2.IR_full       1   ATTCCCCAGCAAACCGATCAGTTATACCAGCACCAATACTTCCGCCAAAACCTATTATCT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10901R-2.in silico     1   ATTCCCCAGCAAACCGATCAGTTATACCAGCACCAATACTTCCGCCAAAACCTATTATCT 60


10901R-2.IR_full       61  TAAGTCCGTGAAAAAGCGGGTGACCACGTGTTTCCAGCAGTTGCGCNGATAAACTCCAGT 120
                           |||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||
10901R-2.in silico     61  TAAGTCCGTGAAAAAGCGGGTGACCACGTGTTTCCAGCAGTTGCGC-GATAAACTCCAGT 120


10901R-2.IR_full       121 CATCCGGTTCCTTTCGCAAGAGTTCCTCCAGCTGCCTCAACCAGATCTTCGTAAGGTCCG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10901R-2.in silico     121 CATCCGGTTCCTTTCGCAAGAGTTCCTCCAGCTGCCTCAACCAGATCTTCGTAAGGTCCG 180


10901R-2.IR_full       181 ATTTCTCCGCATGCGGCGAACGCTTTCGGAAGATCTTCAAATCGGCGCGAAAAACCGAAT 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10901R-2.in silico     181 ATTTCTCCGCATGCGGCGAACGCTTTCGGAAGATCTTCAAATCGGCGCGAAAAACCGAAT 240


10901R-2.IR_full       241 TGCCGGAGTTGTGGAAGGTGCCATTGGTGGCCCATGAACTCACCAGCCGGCAGAGCAGCC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10901R-2.in silico     241 TGCCGGAGTTGTGGAAGGTGCCATTGGTGGCCCATGAACTCACCAGCCGGCAGAGCAGCC 300


10901R-2.IR_full       301 AGCAGTTGCAAGTCGTAGCCAGACTCTTCTCGTCCACTCAGATTTCCACCAAGGAAATCA 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10901R-2.in silico     301 AGCAGTTGCAAGTCGTAGCCAGACTCTTCTCGTCCACTCAGATTTCCACCAAGGAAATCA 360


10901R-2.IR_full       361 CCTACAACAGCAACAGCAACACCAGCGAGAACAACATGACCATCATCGAGAGCAACTATA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10901R-2.in silico     361 CCTACAACAGCAACAGCAACACCAGCGAGAACAACATGACCATCATCGAGAGCAACTATA 420


10901R-2.IR_full       421 TATCCGTGCGCGAGGAATATCCCGATATAGATAGTGAGGTGCGCGCCATATTGCTGAGCC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10901R-2.in silico     421 TATCCGTGCGCGAGGAATATCCCGATATAGATAGTGAGGTGCGCGCCATATTGCTGAGCC 480


10901R-2.IR_full       481 ACGCCCAGAATGGAATCACGATATCGAGCATCAAGAGTGA 520
                           ||||||||||||||||||||||||||||||||||||||||
10901R-2.in silico     481 ACGCCCAGAATGGAATCACGATATCGAGCATCAAGAGTGA 520

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	501	0	0	2	NM_169248.2	CG10901-RA, transcript variant A (osk), mRNA
100	501	0	0	2	NM_206464.1	CG10901-RC, transcript variant C (osk), mRNA
0.79	4	3	14	82	NM_080364.3	CG5461-RA, transcript variant A (bun), mRNA
0.59	3	4	7	48	NM_165853.1	CG13188-RA, transcript variant A (CG13188), mRNA
0.59	3	4	7	29	NM_136870.1	CG13188-RB, transcript variant B (CG13188), mRNA
0.39	2	7	28	82	NM_057376.2	CG7734-RC, transcript variant C (shn), mRNA
0.39	2	7	28	82	NM_134279.2	CG7734-RB, transcript variant B (shn), mRNA
0.39	2	7	28	82	NM_057375.3	CG7734-RA, transcript variant A (shn), mRNA
0.39	2	7	28	82	NM_206091.1	CG7734-RD, transcript variant D (shn), mRNA
0.39	2	6	35	73	NM_132235.2	CG32717-RB, transcript variant B (sdt), mRNA
0.39	2	4	29	63	NM_132236.3	CG32717-RA, transcript variant A (sdt), mRNA
0.39	2	4	29	63	NM_206653.1	CG32717-RE, transcript variant E (sdt), mRNA
0.19	1	10	8	40	NM_057895.3	CG14938-RA, transcript variant A (crol), mRNA
0.19	1	10	8	40	NM_057897.3	CG14938-RB, transcript variant B (crol), mRNA
0.19	1	10	8	40	NM_164971.1	CG14938-RD, transcript variant D (crol), mRNA
0.19	1	10	8	40	NM_057896.3	CG14938-RC, transcript variant C (crol), mRNA
0.19	1	5	35	99	NM_206620.4	CG32782-RD, transcript variant D (tlk), mRNA
0.19	1	5	35	99	NM_130717.3	CG32782-RC, transcript variant C (tlk), mRNA
0.19	1	5	19	55	NM_206303.1	CG6964-RD, transcript variant D (Gug), mRNA
0.19	1	5	17	44	NM_176300.1	CG6964-RC, transcript variant C (Gug), mRNA
0.19	1	5	17	44	NM_079249.2	CG6964-RB, transcript variant B (Gug), mRNA
0.19	1	5	17	44	NM_145106.1	CG6964-RA, transcript variant A (Gug), mRNA
0.19	1	3	2	3	NM_140708.2	CG6485-RA (CG6485), mRNA
0.19	1	2	2	8	NM_135894.2	CG15270-RA, transcript variant A (CG15270), mRNA
0.19	1	2	2	8	NM_205997.1	CG15270-RB, transcript variant B (CG15270), mRNA
0	0	21	67	155	NM_132288.1	CG15365-RA (CG15365), mRNA
0	0	13	35	98	NM_079508.2	CG2534-RA, transcript variant A (cno), mRNA
0	0	13	35	98	NM_169025.1	CG2534-RB, transcript variant B (cno), mRNA
0	0	12	66	133	NM_139493.2	CG2083-RA (CG2083), mRNA
0	0	12	38	51	NM_136647.2	CG8809-RA (Camta), mRNA

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