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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 10742R-2

Symbol Tsp3A Full Name Tetraspanin 3A

CG No CG10742 Old CG No CG10742

Synonyms CG10742, BACR7C10.6, EG:BACR7C10.6, Dm.Tsp3A, Tsp3A

Accession No (Link to NCBI) NM_080315.2

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCTACCGATATCAGGGTGCTGG

Primer Seq. 3'

aatctagaggtaccGATCGTCGCGGTACGAGTGGA

Predicted Fragment Size

IR fragment full Seq

0001 CTACCGATAT CAGGGTGCTG GGNTCGGGGG CGTCGGCATG GCCGNGNGGC GTGGCTACAG 
0061 CGGCATCGAG GTGCACGAGG TGATGCATCC GCATCACTTC ACCTACGTGA GCCAGTGCGT 
0121 CAAGTACATG ATCTTCCTGC TGAACTTCGT GTTCTGGCTC TTTGGCGGCC TGCTCCTGGG 
0181 CATTGGCGTC TATGCGTTCA GGGACAAGTG GGAGGACGCG AACGGATCGG TGCGTNNTNG 
0241 AGAACTTCTA CGACGTATTC CTCAATATCT CGCTGGTGAT GATCCTGGCC GCGCACGGTC 
0301 ATCATTTCTG GTCAGCTTCT CCGGCTGCGT gNGNNNACTG CGCGAGAACA CATTCCTACT 
0361 GAAGTTCTAC TCCATGTGCC TGCTGCTTTT CTTCCTGCTG GAGATGGCCA TCGCCATCGT 
0421 CTGCTTCGTG TGCCCGCAGT ACATGAACAC NCNTTTTGGA GAAGCAGTTC ACGCACAAGA 
0481 TCATCCACTC GTACCGCGAC GATC

in silico PCR Fragment

0001 CTACCGATAT CAGGGTGCTG GGCTCGGGGG CGTCGGCATG GCCGGGGGGC GTGGCTACAG 
0061 CGGCATCGAG GTGCACGAGG TGATGCATCC GCATCACTTC ACCTACGTGA GCCAGTGCGT 
0121 CAAGTACATG ATCTTCCTGC TGAACTTCGT GTTCTGGCTC TTTGGCGGCC TGCTCCTGGG 
0181 CATTGGCGTC TATGCGTTCA GGGACAAGTG GGAGGACGCG AACGGATCGG TGCG-GCTGG 
0241 AGAACTTCTA CGACGTATTC CTCAATATCT CGCTGGTGAT GATCCTGGCC G-GCACGGTC 
0301 ATC-TTTCTG GTCAGCTTCT CCGGCTGCGT GGGCGCACTG CGCGAGAACA CATTCCTACT 
0361 GAAGTTCTAC TCCATGTGCC TGCTGCTTTT CTTCCTGCTG GAGATGGCCA TCGCCATCGT 
0421 CTGCTTCGTG TGCCCGCAGT ACATGAACAC -CTTTTTGGA GAAGCAGTTC ACGCACAAGA 
0481 TCATCCACTC GTACCGCGAC GATC

Assemble Data


10742R-2.IR_full       1   CTACCGATATCAGGGTGCTGGGNTCGGGGGCGTCGGCATGGCCGNGNGGCGTGGCTACAG 60
                           |||||||||||||||||||||| ||||||||||||||||||||| | |||||||||||||
10742R-2.in silico     1   CTACCGATATCAGGGTGCTGGGCTCGGGGGCGTCGGCATGGCCGGGGGGCGTGGCTACAG 60


10742R-2.IR_full       61  CGGCATCGAGGTGCACGAGGTGATGCATCCGCATCACTTCACCTACGTGAGCCAGTGCGT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10742R-2.in silico     61  CGGCATCGAGGTGCACGAGGTGATGCATCCGCATCACTTCACCTACGTGAGCCAGTGCGT 120


10742R-2.IR_full       121 CAAGTACATGATCTTCCTGCTGAACTTCGTGTTCTGGCTCTTTGGCGGCCTGCTCCTGGG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10742R-2.in silico     121 CAAGTACATGATCTTCCTGCTGAACTTCGTGTTCTGGCTCTTTGGCGGCCTGCTCCTGGG 180


10742R-2.IR_full       181 CATTGGCGTCTATGCGTTCAGGGACAAGTGGGAGGACGCGAACGGATCGGTGCGTNNTNG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||   | |
10742R-2.in silico     181 CATTGGCGTCTATGCGTTCAGGGACAAGTGGGAGGACGCGAACGGATCGGTGCG-GCTGG 240


10742R-2.IR_full       241 AGAACTTCTACGACGTATTCCTCAATATCTCGCTGGTGATGATCCTGGCCGCGCACGGTC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||
10742R-2.in silico     241 AGAACTTCTACGACGTATTCCTCAATATCTCGCTGGTGATGATCCTGGCCG-GCACGGTC 300


10742R-2.IR_full       301 ATCATTTCTGGTCAGCTTCTCCGGCTGCGTGNGNNNACTGCGCGAGAACACATTCCTACT 360
                           ||| ||||||||||||||||||||||||||| |   ||||||||||||||||||||||||
10742R-2.in silico     301 ATC-TTTCTGGTCAGCTTCTCCGGCTGCGTGGGCGCACTGCGCGAGAACACATTCCTACT 360


10742R-2.IR_full       361 GAAGTTCTACTCCATGTGCCTGCTGCTTTTCTTCCTGCTGGAGATGGCCATCGCCATCGT 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10742R-2.in silico     361 GAAGTTCTACTCCATGTGCCTGCTGCTTTTCTTCCTGCTGGAGATGGCCATCGCCATCGT 420


10742R-2.IR_full       421 CTGCTTCGTGTGCCCGCAGTACATGAACACNCNTTTTGGAGAAGCAGTTCACGCACAAGA 480
                           |||||||||||||||||||||||||||||| | |||||||||||||||||||||||||||
10742R-2.in silico     421 CTGCTTCGTGTGCCCGCAGTACATGAACAC-CTTTTTGGAGAAGCAGTTCACGCACAAGA 480


10742R-2.IR_full       481 TCATCCACTCGTACCGCGACGATC 504
                           ||||||||||||||||||||||||
10742R-2.in silico     481 TCATCCACTCGTACCGCGACGATC 504

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	4	NM_080315.2	CG10742-RA (Tsp3A), mRNA
1.65	8	32	77	65	NM_079585.2	CG4591-RA (Tsp86D), mRNA
0	0	4	3	2	NM_165005.1	CG31762-RD, transcript variant D (aret), mRNA
0	0	3	7	7	NM_132290.1	CG7267-RB (CG7267), mRNA
0	0	3	3	4	NM_165233.1	CG5674-RC, transcript variant C (CG5674), mRNA
0	0	3	3	4	NM_136012.4	CG5674-RA, transcript variant A (CG5674), mRNA
0	0	3	3	4	NM_165232.1	CG5674-RB, transcript variant B (CG5674), mRNA
0	0	3	3	3	NM_170095.1	CG31141-RA (CG31141), mRNA
0	0	3	2	6	NM_167240.1	CG1799-RA, transcript variant A (ras), mRNA
0	0	3	2	6	NM_079907.4	CG1799-RB, transcript variant B (ras), mRNA
0	0	3	2	6	NM_167241.1	CG1799-RC, transcript variant C (ras), mRNA
0	0	3	2	4	NM_168695.2	CG11905-RB, transcript variant B (CG11905), mRNA
0	0	3	2	4	NM_168696.2	CG11905-RF, transcript variant F (CG11905), mRNA
0	0	3	2	2	NM_144110.1	CG16836-RA (CG16836), mRNA
0	0	2	5	3	NM_078543.2	CG15793-RA (Dsor1), mRNA
0	0	2	4	7	NM_134787.2	CG31670-RA (CG31670), mRNA
0	0	2	3	5	NM_139421.3	CG5687-RA (CG5687), mRNA
0	0	2	3	4	NM_079974.2	CG3870-RA (chrw), mRNA
0	0	2	3	2	NM_164379.1	CG31920-RB (CG31920), mRNA
0	0	2	2	3	NM_170320.1	CG31065-RA (CG31065), mRNA
0	0	1	5	3	NM_166569.1	CG12781-RB, transcript variant B (nahoda), mRNA
0	0	1	5	3	NM_079091.2	CG12781-RA, transcript variant A (nahoda), mRNA
0	0	1	4	9	NM_167188.1	CG32703-RA (CG32703), mRNA
0	0	1	4	4	NM_139394.1	CG18171-RA (CG18171), mRNA
0	0	1	4	3	NM_132573.1	CG17788-RA (CG17788), mRNA
0	0	1	3	6	NM_135764.3	CG5287-RA (CG5287), mRNA
0	0	1	3	5	NM_176494.2	CG10851-RG, transcript variant G (B52), mRNA
0	0	1	3	5	NM_176492.2	CG10851-RD, transcript variant D (B52), mRNA
0	0	1	3	5	NM_176493.2	CG10851-RF, transcript variant F (B52), mRNA
0	0	1	3	5	NM_001014619.1	CG10851-RH, transcript variant H (B52), mRNA

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