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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

10738R-1

Symbol

CG10738

Full Name

CG10738

CG No

CG10738

Old CG No

CG10738

Synonyms

GYC, CG10738

Accession No (Link to NCBI)

NM_168547.1

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Swarup S, Pradhan-Sundd T, Verheyen EM.<br> Genome-wide identification of phospho-regulators of Wnt signaling in Drosophila.<br> Development (2015) 142(8) 1502-15 [ PubMed ID = 25852200 ] [ RRC reference ]

Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTGTCCTGCTCCAGCTGGAAAC

Primer Seq. 3'

aatctagaggtaccACTACGGACTTGGAGATCTGC

Predicted Fragment Size

IR fragment full Seq

0001 TGTCCTGCTC CAGCTGGAAA CTACCACTCA GGACTGCTGC ATCCACTGCT CCTGCTCCTC 
0061 TTTCTGCTCG CTTTTAGCAA CTTCCGGCCG ACTCATGGAG AGGTCTTCAC GCTGGGTTAC 
0121 CTCACGGCCT CGCAACGGCG GCCGGGNAAA CCTGGACTAC AACCGACCCG GTCTCACCAT 
0181 ATCACGGCGC CATCTCGCTG GCGGTGGAGG AGGTGAATGC CGGACGACTC AGGGATCGGG 
0241 GTCATTCCCT TCAGTTCGTC GTGGCCGAAA CTTACGGTGA GGAGGTGGTC AGCATCCGTC 
0301 AAACGGCGGC ACTGTGGACG CAGCAGGTGG CCGCCTATAT TGGCCCCCAG GANAACTTGC 
0361 GTACACGAAG GTCGCATNGC GGCCGCCTTT AACCTGCCCA TGATATCTTA TTACTGCANC 
0421 CATCGGGATC CCTCGAACAA GGCTGATTTT CCCACCTTTG CCAGGACACg NCCACCGGAC 
0481 ACGCAGATCT CCAAGTCCGT AGT

in silico PCR Fragment

0001 TGTCCTGCTC CAGCTGGAAA CTACCACTCA GGACTGCTGC ATCCACTGCT CCTGCTCCTC 
0061 TTTCTGCTCG CTTTTAGCAA CTTCCGGCCG ACTCATGGAG AGGTCTTCAC GCTGGGTTAC 
0121 CTCACGGCCT CGCAACGGCG GCCGGG-AAA CCTGGACTAC AACCGACCCG GTCTCACCAT 
0181 ATC-CGGCGC CATCTCGCTG GCGGTGGAGG AGGTGAATGC CGGACGACTC AGGGATCGGG 
0241 GTCATTCCCT TCAGTTCGTC GTGGCCGAAA CTTACGGTGA GGAGGTGGTC AGCATCCGTC 
0301 AAACGGCGGC ACTGTGGACG CAGCAGGTGG CCGCCTATAT TGGCCCCCAG GA-AACTTGC 
0361 GTACACGAAG GTCGCATGGC GGCCGCCTTT AACCTGCCCA TGATATCTTA TTACTGCACC 
0421 CATCGGGATC CCTCGAACAA GGCTGATTTT CCCACCTTTG CCAGGACACG TCCACCGGAC 
0481 ACGCAGATCT CCAAGTCCGT AGT

Assemble Data

<PRE>

10738R-1.IR_full       1   TGTCCTGCTCCAGCTGGAAACTACCACTCAGGACTGCTGCATCCACTGCTCCTGCTCCTC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10738R-1.in silico     1   TGTCCTGCTCCAGCTGGAAACTACCACTCAGGACTGCTGCATCCACTGCTCCTGCTCCTC 60


10738R-1.IR_full       61  TTTCTGCTCGCTTTTAGCAACTTCCGGCCGACTCATGGAGAGGTCTTCACGCTGGGTTAC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10738R-1.in silico     61  TTTCTGCTCGCTTTTAGCAACTTCCGGCCGACTCATGGAGAGGTCTTCACGCTGGGTTAC 120


10738R-1.IR_full       121 CTCACGGCCTCGCAACGGCGGCCGGG<span class="snp"><tt>N</tt></span>AAACCTGGACTACAACCGACCCGGTCTCACCAT 180
                           ||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||
10738R-1.in silico     121 CTCACGGCCTCGCAACGGCGGCCGGG<span class="snp"><tt>-</tt></span>AAACCTGGACTACAACCGACCCGGTCTCACCAT 180


10738R-1.IR_full       181 ATC<span class="snp"><tt>A</tt></span>CGGCGCCATCTCGCTGGCGGTGGAGGAGGTGAATGCCGGACGACTCAGGGATCGGG 240
                           |||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10738R-1.in silico     181 ATC<span class="snp"><tt>-</tt></span>CGGCGCCATCTCGCTGGCGGTGGAGGAGGTGAATGCCGGACGACTCAGGGATCGGG 240


10738R-1.IR_full       241 GTCATTCCCTTCAGTTCGTCGTGGCCGAAACTTACGGTGAGGAGGTGGTCAGCATCCGTC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10738R-1.in silico     241 GTCATTCCCTTCAGTTCGTCGTGGCCGAAACTTACGGTGAGGAGGTGGTCAGCATCCGTC 300


10738R-1.IR_full       301 AAACGGCGGCACTGTGGACGCAGCAGGTGGCCGCCTATATTGGCCCCCAGGA<span class="snp"><tt>N</tt></span>AACTTGC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||
10738R-1.in silico     301 AAACGGCGGCACTGTGGACGCAGCAGGTGGCCGCCTATATTGGCCCCCAGGA<span class="snp"><tt>-</tt></span>AACTTGC 360


10738R-1.IR_full       361 GTACACGAAGGTCGCAT<span class="snp"><tt>N</tt></span>GCGGCCGCCTTTAACCTGCCCATGATATCTTATTACTGCA<span class="snp"><tt>N</tt></span>C 420
                           |||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|
10738R-1.in silico     361 GTACACGAAGGTCGCAT<span class="snp"><tt>G</tt></span>GCGGCCGCCTTTAACCTGCCCATGATATCTTATTACTGCA<span class="snp"><tt>C</tt></span>C 420


10738R-1.IR_full       421 CATCGGGATCCCTCGAACAAGGCTGATTTTCCCACCTTTGCCAGGACACG<span class="snp"><tt>N</tt></span>CCACCGGAC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||
10738R-1.in silico     421 CATCGGGATCCCTCGAACAAGGCTGATTTTCCCACCTTTGCCAGGACACG<span class="snp"><tt>T</tt></span>CCACCGGAC 480


10738R-1.IR_full       481 ACGCAGATCTCCAAGTCCGTAGT 503
                           |||||||||||||||||||||||
10738R-1.in silico     481 ACGCAGATCTCCAAGTCCGTAGT 503

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_140396.1	CG10738-RB, transcript variant B (CG10738), mRNA
100	482	0	0	0	NM_168547.1	CG10738-RA, transcript variant A (CG10738), mRNA
0	0	5	3	3	NM_170557.1	CG11525-RD, transcript variant D (CycG), mRNA
0	0	5	3	3	NM_170555.1	CG11525-RB, transcript variant B (CycG), mRNA
0	0	5	3	3	NM_079870.2	CG11525-RA, transcript variant A (CycG), mRNA
0	0	5	3	3	NM_170556.1	CG11525-RC, transcript variant C (CycG), mRNA
0	0	3	4	4	NM_132959.2	CG8949-RA (CG8949), mRNA
0	0	3	2	2	NM_079051.2	CG4903-RA (MESR4), mRNA
0	0	3	2	2	NM_144343.2	CG8250-RA (Alk), mRNA
0	0	3	2	2	NM_135892.2	CG4185-RA (NC2beta), mRNA
0	0	2	6	8	NM_132734.2	CG9411-RA (CG9411), mRNA
0	0	2	3	10	NM_139458.2	CG1317-RB (CG1317), mRNA
0	0	2	3	7	NM_142055.1	CG9322-RA (CG9322), mRNA
0	0	2	3	3	NM_143811.2	CG5704-RA (CG5704), mRNA
0	0	2	2	2	NM_079476.2	CG5683-RB, transcript variant B (Aef1), mRNA
0	0	2	2	2	NM_142359.2	CG31256-RA (Brf), mRNA
0	0	2	2	2	NM_168904.2	CG5683-RC, transcript variant C (Aef1), mRNA
0	0	2	2	2	NM_168903.2	CG5683-RA, transcript variant A (Aef1), mRNA
0	0	2	2	2	NM_135717.2	CG6504-RA (Pkd2), mRNA
0	0	2	2	2	NM_079296.2	CG8019-RA (hay), mRNA
0	0	1	6	8	NM_134512.4	CG12701-RA, transcript variant A (CG12701), mRNA
0	0	1	6	8	NM_167685.1	CG12701-RB, transcript variant B (CG12701), mRNA
0	0	1	4	5	NM_141666.2	CG9492-RA (CG9492), mRNA
0	0	1	4	4	NM_001042838.1	CG40500-RC, transcript variant C (CG40500), mRNA
0	0	1	4	4	NM_001042840.1	CG40500-RA, transcript variant A (CG40500), mRNA
0	0	1	4	4	NM_001042839.1	CG40500-RB, transcript variant B (CG40500), mRNA
0	0	1	4	4	NM_001042841.1	CG40500-RD, transcript variant D (CG40500), mRNA
0	0	1	3	11	NM_001043216.1	CG34113-RO, transcript variant O (CG34113), mRNA
0	0	1	3	10	NM_132288.1	CG15365-RA (CG15365), mRNA
0	0	1	3	6	NM_001043217.1	CG34113-RP, transcript variant P (CG34113), mRNA

Wing Disc

GFP

Puc

Caspase

DAPI

Merge

Figure

JNK signal

Caspase-3 signal

Dcompartment		Penetrance

Pathways (updated: 2024/04/26)

KEGG Pathway

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