NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

10712-1R-1

Symbol

Chro

Full Name

Chromator

CG No

CG10712

Old CG No

CG10712

Synonyms

Chriz, CG10712, unnamed, anon-EST:fe2E3, Chro

Accession No (Link to NCBI)

NM_141169.2

Inserted Chr.

lll

Insertional Mutation

lethal

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTGGACGTTCTTGTATGTGGAC

Primer Seq. 3'

aatctagaggtaccTGAGGAAGGCGTTGGTGTACT

Predicted Fragment Size

IR fragment full Seq

0001 TNGACGTTCT TGTATgTGGA CGATGTCTCC GGGCCTACAA CTTCGTGGAA GAGTTTCAGG 
0061 CCCACAAAGA GGACGCCTGC GAAAAGGAAA ATGCAAATTT GAAGGAATCG CTCGATCCCA 
0121 AGCCCACCAT CTGGGCTTTT ACGCTATGGA AAGCTACTCA GCTGCACTCG CGCAAGGACG 
0181 CGTCCGGCAA CTCGTGGGCC TTGTACCAGC ATTGGGTAAA ACTAGACGAT AGCGTCCGCG 
0241 AAGCCGTGGA TTGTGGCTGG GAAGACCATA CAGTCATTCG GCAAAATTGC ACACGGACAA 
0301 CTCCAGGACA TGCCTGTGCG AATAACAAAG ACTGTGGTCA ATCCAAACAA TAACAACACA 
0361 TCAAATAGCA ACAACAACAA CACGAGCGTC TCCCCTACCA GAAAATCACC AACCGGTAAG 
0421 CTGCCTACGT TAGCCAATCA ACTAAAGGAT ACAGAAAACG AAAGACCCAA GTCCAAGCCG 
0481 AGTACACCAA CGCCTTCCTC A

in silico PCR Fragment

0001 TGGACGTTCT TGTATGTGGA CGATGTCTCC GGGCCTACAA CTTCGTGGAA GAGTTTCAGG 
0061 CCCACAAAGA GGACGCCTGC GAAAAGGAAA ATGCAAATTT GAAGGAATCG CTCGATCCCA 
0121 AGCCCACCAT CTGGGCTTTT ACGCTATGGA AAGCTACTCA GCTGCACTCG CGCAAGGACG 
0181 CGTCCGGCAG CTCGTGGGCC TTGTACCAGC ATTGGGTAAA ACTAGACGAT AGCGTCCGCG 
0241 -AGCCGTGGA TTGTGGCTGG GAAGACCATA CAGTCATTCG GCAAAATTGC ACACGGACAA 
0301 CTCCAGGACA TGCCTGTGCG AATAACAAAG ACTGTGGTCA ATCCAAACAA TAACAACACA 
0361 TCAAATAGCA ACAACAACAA CACGAGCGTC TCCCCTACCA GAAAATCACC AACCGGTAAG 
0421 CTGCCTACGT TAGCCAATCA ACTAAAGGAT ACAGAAAACG AAAGACCCAA GTCCAAGCCG 
0481 AGTACACCAA CGCCTTCCTC A

Assemble Data

<PRE>

10712-1R-1.IR_full       1   T<span class="snp"><tt>N</tt></span>GACGTTCTTGTATGTGGACGATGTCTCCGGGCCTACAACTTCGTGGAAGAGTTTCAGG 60
                             |<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10712-1R-1.in silico     1   T<span class="snp"><tt>G</tt></span>GACGTTCTTGTATGTGGACGATGTCTCCGGGCCTACAACTTCGTGGAAGAGTTTCAGG 60


10712-1R-1.IR_full       61  CCCACAAAGAGGACGCCTGCGAAAAGGAAAATGCAAATTTGAAGGAATCGCTCGATCCCA 120
                             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10712-1R-1.in silico     61  CCCACAAAGAGGACGCCTGCGAAAAGGAAAATGCAAATTTGAAGGAATCGCTCGATCCCA 120


10712-1R-1.IR_full       121 AGCCCACCATCTGGGCTTTTACGCTATGGAAAGCTACTCAGCTGCACTCGCGCAAGGACG 180
                             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10712-1R-1.in silico     121 AGCCCACCATCTGGGCTTTTACGCTATGGAAAGCTACTCAGCTGCACTCGCGCAAGGACG 180


10712-1R-1.IR_full       181 CGTCCGGCA<span class="snp"><tt>A</tt></span>CTCGTGGGCCTTGTACCAGCATTGGGTAAAACTAGACGATAGCGTCCGCG 240
                             |||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||||||||
10712-1R-1.in silico     181 CGTCCGGCA<span class="snp"><tt>G</tt></span>CTCGTGGGCCTTGTACCAGCATTGGGTAAAACTAGACGATAGCGTCCGCG 240


10712-1R-1.IR_full       241 <span class="snp"><tt>A</tt></span>AGCCGTGGATTGTGGCTGGGAAGACCATACAGTCATTCGGCAAAATTGCACACGGACAA 300
                             <span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10712-1R-1.in silico     241 <span class="snp"><tt>-</tt></span>AGCCGTGGATTGTGGCTGGGAAGACCATACAGTCATTCGGCAAAATTGCACACGGACAA 300


10712-1R-1.IR_full       301 CTCCAGGACATGCCTGTGCGAATAACAAAGACTGTGGTCAATCCAAACAATAACAACACA 360
                             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10712-1R-1.in silico     301 CTCCAGGACATGCCTGTGCGAATAACAAAGACTGTGGTCAATCCAAACAATAACAACACA 360


10712-1R-1.IR_full       361 TCAAATAGCAACAACAACAACACGAGCGTCTCCCCTACCAGAAAATCACCAACCGGTAAG 420
                             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10712-1R-1.in silico     361 TCAAATAGCAACAACAACAACACGAGCGTCTCCCCTACCAGAAAATCACCAACCGGTAAG 420


10712-1R-1.IR_full       421 CTGCCTACGTTAGCCAATCAACTAAAGGATACAGAAAACGAAAGACCCAAGTCCAAGCCG 480
                             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10712-1R-1.in silico     421 CTGCCTACGTTAGCCAATCAACTAAAGGATACAGAAAACGAAAGACCCAAGTCCAAGCCG 480


10712-1R-1.IR_full       481 AGTACACCAACGCCTTCCTCA 501
                             |||||||||||||||||||||
10712-1R-1.in silico     481 AGTACACCAACGCCTTCCTCA 501

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	2	NM_168975.1	CG10712-RB, transcript variant B (Chro), mRNA
100	482	0	0	2	NM_168976.1	CG10712-RC, transcript variant C (Chro), mRNA
100	482	0	0	1	NM_141169.2	CG10712-RA, transcript variant A (Chro), mRNA
0.2	1	3	10	39	NM_136854.1	CG13194-RA (pyr), mRNA
0.2	1	3	3	13	NM_143154.3	CG5053-RA (CG5053), mRNA
0.2	1	3	3	2	NM_169294.1	CG9399-RB, transcript variant B (CG9399), mRNA
0.2	1	3	3	2	NM_141656.1	CG9399-RA, transcript variant A (CG9399), mRNA
0.2	1	2	4	7	NM_205989.2	CG4551-RB, transcript variant B (smi35A), mRNA
0.2	1	2	4	7	NM_078840.4	CG4551-RA, transcript variant A (smi35A), mRNA
0.2	1	2	4	7	NM_205988.2	CG4551-RC, transcript variant C (smi35A), mRNA
0.2	1	2	4	7	NM_001038818.1	CG4551-RE, transcript variant E (smi35A), mRNA
0.2	1	2	4	7	NM_001038817.1	CG4551-RD, transcript variant D (smi35A), mRNA
0.2	1	2	3	7	NM_165017.1	CG12287-RB, transcript variant B (pdm2), mRNA
0	0	6	23	90	NM_167335.1	CG4013-RC, transcript variant C (Smr), mRNA
0	0	6	23	90	NM_167334.1	CG4013-RB, transcript variant B (Smr), mRNA
0	0	6	23	90	NM_080536.2	CG4013-RA, transcript variant A (Smr), mRNA
0	0	4	11	21	NM_132538.1	CG15737-RA (CG15737), mRNA
0	0	3	15	35	NM_001042820.1	CG34145-RA (CG34145), mRNA
0	0	3	7	16	NM_167685.1	CG12701-RB, transcript variant B (CG12701), mRNA
0	0	3	7	16	NM_134512.4	CG12701-RA, transcript variant A (CG12701), mRNA
0	0	3	7	9	NM_133043.2	CG5744-RB, transcript variant B (Frq1), mRNA
0	0	3	7	9	NM_001038764.1	CG5744-RC, transcript variant C (Frq1), mRNA
0	0	3	4	4	NM_079055.2	CG4943-RA (lack), mRNA
0	0	3	3	19	NM_057539.3	CG9310-RA, transcript variant A (Hnf4), mRNA
0	0	2	13	58	NM_132370.1	CG2989-RA (CG2989), mRNA
0	0	2	7	19	NM_079445.2	CG8567-RA, transcript variant A (Deaf1), mRNA
0	0	2	7	19	NM_001038944.1	CG8567-RB, transcript variant B (Deaf1), mRNA
0	0	2	7	18	NM_169565.1	CG9924-RA, transcript variant A (CG9924), mRNA
0	0	2	7	18	NM_001038965.1	CG9924-RE, transcript variant E (CG9924), mRNA
0	0	2	7	18	NM_169564.1	CG9924-RD, transcript variant D (CG9924), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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