NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

10672R-1

Symbol

CG10672

Full Name

CG10672

CG No

CG10672

Old CG No

CG10672

Synonyms

CG10672

Accession No (Link to NCBI)

NM_139689.2

Inserted Chr.

lll

Insertional Mutation

lethal

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTGAGCAAACAACTGGTGGTCA

Primer Seq. 3'

aatctagaggtaccCTCATCGCACTCGAGGACACC

Predicted Fragment Size

IR fragment full Seq

0001 TGAGCAAACA ACTGGTGGTC AGGGCGCCCA AGATCCGTCT GAATGCCAGC GCTGTGAGTG 
0061 GAAGTGGTCA AAGTTCAAGT TTCGATCAAA ACAGCAACAA TTACAGCCGA AAACTTGTTG 
0121 GCCCTAATCT CAATCAATGC CACAAACGCT TGTCCAGCAG TAGTCAAAGT TCGACAGCCG 
0181 GCACGATGAA GCGTTTGGCA GGAAAAGTAG CCGTGGTCAC CGCCTCCACC GATGGCATTG 
0241 GTTTCGCGAT TGCCAAAAGG CTGGCCGAAG ATGGCGCCGC AGTGGTCATC AGCAGTCGCA 
0301 AGCAAAAGAA TGTGGACTCT GCTCTGGCGG AgCTGCCGCA AACTGAACTT GAATGTCCAC 
0361 GGTCTGAAGT GCCATGTCAG TGAACCCGAG GATCGCAAAC AGCTCTTTGA GGAGACCATT 
0421 AGCAAGTTTG GCAAGCTTAA CATTCTGGTC AGCAATGCAG CCACCAATCc TGCGGTCGGC 
0481 GGTGTCCTCG AGTGCGATGA G

in silico PCR Fragment

0001 TGAGCAAACA ACTGGTGGTC AGGGCGCCCA AGATCCGTCT GAATGCCAGC GCTGTGAGTG 
0061 GAAGTGGTCA AAGTTCAAGT TTCGATCAAA ACAGCAACAA TTACAGCCGA AAACTTGTTG 
0121 GCCCTAATCT CAATCAATGC CACAAACGCT TGTCCAGCAG TAGTCAAAGT TCGACAGCCG 
0181 GCACGATGAA GCGTTTGGCA GGAAAAGTAG CCGTGGTCAC CGCCTCCACC GATGGCATTG 
0241 GTTTCGCGAT TGCCAAAAGG CTGGCCGAAG ATGGCGCCGC AGTGGTCATC AGCAGTCGCA 
0301 AGCAAAAGAA TGTGGACTCT GCTCTGGCGG AGCTG-CGCA AACTGAACTT GAATGTCCAC 
0361 GGTCTGAAGT GCCATGTCAG CGAACCCGAG GATCGCAAAC AGCTCTTTGA GGAGACCATT 
0421 AGCAAGTTTG GCAAGCTCAA CATTCTGGTC AGCAATGCAG CCACCAATCC TGCGGTCGGC 
0481 GGTGTCCTCG AGTGCGATGA G

Assemble Data

<PRE>

10672R-1.IR_full       1   TGAGCAAACAACTGGTGGTCAGGGCGCCCAAGATCCGTCTGAATGCCAGCGCTGTGAGTG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10672R-1.in silico     1   TGAGCAAACAACTGGTGGTCAGGGCGCCCAAGATCCGTCTGAATGCCAGCGCTGTGAGTG 60


10672R-1.IR_full       61  GAAGTGGTCAAAGTTCAAGTTTCGATCAAAACAGCAACAATTACAGCCGAAAACTTGTTG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10672R-1.in silico     61  GAAGTGGTCAAAGTTCAAGTTTCGATCAAAACAGCAACAATTACAGCCGAAAACTTGTTG 120


10672R-1.IR_full       121 GCCCTAATCTCAATCAATGCCACAAACGCTTGTCCAGCAGTAGTCAAAGTTCGACAGCCG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10672R-1.in silico     121 GCCCTAATCTCAATCAATGCCACAAACGCTTGTCCAGCAGTAGTCAAAGTTCGACAGCCG 180


10672R-1.IR_full       181 GCACGATGAAGCGTTTGGCAGGAAAAGTAGCCGTGGTCACCGCCTCCACCGATGGCATTG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10672R-1.in silico     181 GCACGATGAAGCGTTTGGCAGGAAAAGTAGCCGTGGTCACCGCCTCCACCGATGGCATTG 240


10672R-1.IR_full       241 GTTTCGCGATTGCCAAAAGGCTGGCCGAAGATGGCGCCGCAGTGGTCATCAGCAGTCGCA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10672R-1.in silico     241 GTTTCGCGATTGCCAAAAGGCTGGCCGAAGATGGCGCCGCAGTGGTCATCAGCAGTCGCA 300


10672R-1.IR_full       301 AGCAAAAGAATGTGGACTCTGCTCTGGCGGAGCTG<span class="snp"><tt>C</tt></span>CGCAAACTGAACTTGAATGTCCAC 360
                           |||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||
10672R-1.in silico     301 AGCAAAAGAATGTGGACTCTGCTCTGGCGGAGCTG<span class="snp"><tt>-</tt></span>CGCAAACTGAACTTGAATGTCCAC 360


10672R-1.IR_full       361 GGTCTGAAGTGCCATGTCAG<span class="snp"><tt>T</tt></span>GAACCCGAGGATCGCAAACAGCTCTTTGAGGAGACCATT 420
                           ||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||
10672R-1.in silico     361 GGTCTGAAGTGCCATGTCAG<span class="snp"><tt>C</tt></span>GAACCCGAGGATCGCAAACAGCTCTTTGAGGAGACCATT 420


10672R-1.IR_full       421 AGCAAGTTTGGCAAGCT<span class="snp"><tt>T</tt></span>AACATTCTGGTCAGCAATGCAGCCACCAATCCTGCGGTCGGC 480
                           |||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||
10672R-1.in silico     421 AGCAAGTTTGGCAAGCT<span class="snp"><tt>C</tt></span>AACATTCTGGTCAGCAATGCAGCCACCAATCCTGCGGTCGGC 480


10672R-1.IR_full       481 GGTGTCCTCGAGTGCGATGAG 501
                           |||||||||||||||||||||
10672R-1.in silico     481 GGTGTCCTCGAGTGCGATGAG 501

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_139689.2	CG10672-RA (CG10672), mRNA
0	0	8	2	2	NM_136552.1	CG8642-RA (CG8642), mRNA
0	0	3	3	7	NM_079437.2	CG32211-RA (Taf6), mRNA
0	0	3	2	2	NM_176009.1	CG33129-RE, transcript variant E (CG33129), mRNA
0	0	2	5	8	NM_144448.2	CG1915-RC, transcript variant C (sls), mRNA
0	0	2	5	2	NM_169651.2	CG6156-RA, transcript variant A (CG6156), mRNA
0	0	2	5	2	NM_142199.3	CG6156-RB, transcript variant B (CG6156), mRNA
0	0	2	4	5	NM_079937.2	CG1915-RA, transcript variant A (sls), mRNA
0	0	2	3	3	NM_140083.1	CG18179-RA (CG18179), mRNA
0	0	2	2	5	NM_137768.1	CG13492-RA, transcript variant A (CG13492), mRNA
0	0	2	2	5	NM_166487.2	CG13492-RB, transcript variant B (CG13492), mRNA
0	0	1	5	18	NM_166019.1	CG18076-RH, transcript variant H (shot), mRNA
0	0	1	5	15	NM_166018.1	CG18076-RC, transcript variant C (shot), mRNA
0	0	1	5	14	NM_166015.1	CG18076-RG, transcript variant G (shot), mRNA
0	0	1	5	14	NM_166016.1	CG18076-RB, transcript variant B (shot), mRNA
0	0	1	5	14	NM_166017.1	CG18076-RE, transcript variant E (shot), mRNA
0	0	1	5	14	NM_079009.2	CG18076-RA, transcript variant A (shot), mRNA
0	0	1	5	10	NM_168964.2	CG11352-RB, transcript variant B (jim), mRNA
0	0	1	5	10	NM_143699.2	CG11352-RC, transcript variant C (jim), mRNA
0	0	1	5	7	NM_131931.1	CG12692-RB (CG12692), mRNA
0	0	1	5	2	NM_165525.1	CG11141-RA, transcript variant A (CG11141), mRNA
0	0	1	5	2	NM_136438.2	CG11141-RB, transcript variant B (CG11141), mRNA
0	0	1	4	8	NM_165966.1	CG3991-RD, transcript variant D (TppII), mRNA
0	0	1	4	7	NM_057899.2	CG3991-RA, transcript variant A (TppII), mRNA
0	0	1	3	7	NM_166013.1	CG30485-RA (CG30485), mRNA
0	0	1	3	3	NM_058088.3	CG8730-RA (drosha), mRNA
0	0	1	3	2	NM_136955.2	CG8815-RA, transcript variant A (Sin3A), mRNA
0	0	1	3	2	NM_165916.1	CG8815-RC, transcript variant C (Sin3A), mRNA
0	0	1	3	2	NM_165915.1	CG8815-RB, transcript variant B (Sin3A), mRNA
0	0	1	3	2	NM_165917.1	CG8815-RD, transcript variant D (Sin3A), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

dme00830 : Retinol metabolism
dme01100 : Metabolic pathways
dme04146 : Peroxisome

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