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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

10671R-3

Symbol

CG10671

Full Name

CG10671

CG No

CG10671

Old CG No

CG10671

Synonyms

i136, CG10671

Accession No (Link to NCBI)

NM_139687.2

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTCCGCTGCGTCCAAATTTGGG

Primer Seq. 3'

aatctagaggtaccGTCCGTAGGAGTTCTCCACGA

Predicted Fragment Size

IR fragment full Seq

0001 tCCGCTGCGT CCAAATTTGG GCGGTACTGC GGGAAGTCCC TCCTCCTCCG GTTCCAACAT 
0061 GAATTTCAGG CCCGGAGGAC CTGACATCAC CAGGTCGGAA GCGCGCGGAA CGAGGCCCAC 
0121 GGCCGCACCC ACTAGCATTC GCGAGATCCT GGTCATGGGC GTCATCCATT TGTGCAAGAA 
0181 GACCATATTC TTCAATACGG ACCTCAAAGT AGCCTTGTAT CTGGGCAGTC TGTTTGTGAT 
0241 CTCCGTAATC GGGGACTTTG TGCCCTTCCC CAAGACGTAC TTCGCCCGAT CGGGACAACC 
0301 TGTTCAACCA GTACTTCGTG AAGATCGGCT GGGGCTGGAC GCTGCTCTTC GTGGTGCCCT 
0361 TCCTGGTGCT CTCGGCCTAT ACGATTACGT GTGGCGACCA CAAACGGATG CTGCGAACAC 
0421 CATTTCCCGC GCATCGTCAT AGCCACCTTC TTCTGGTTCT TCTGGACCAA GCTGTTTAAT 
0481 GTCGTGGAGA ACTCCTACGG AC

in silico PCR Fragment

0001 TCCGCTGCGT CCAAATTTGG GCGGTACTGC GGGAAGTCCC TCCTCCTCCG GTTCCAACAT 
0061 GAATTTCAGG CCCGGAGGAC CTGACATCAC CAGGTCGGAA GCGCGCGGAA CGAGGCCCAC 
0121 GGCCGCACCC ACTAGCATCC GCGAGATCCT GGTCATGGGC GTCATCCATT TGTGCAAGAA 
0181 GACCATATTC TTCAATACGG ACCTCAAAGT AGCCTTGTAT CTGGGCAGTC TGTTTGTGAT 
0241 CTCCGTAATC GGGGACTTTG TGCCCTTCCC CAAGACGTAC TTCGCCCGAT C-GGACAACC 
0301 TGTTCAACCA GTACTTCGTG AAGATCGGCT GGGGCTGGAC GCTGCTCTTC GTGGTGCCCT 
0361 TCCTGGTGCT CTCGGCCTAT ACGATTACGT GTGGCGACCA CAAACGGATG CTGCG-ACAC 
0421 CATTTCCCGC GCATCGTCAT AGCCACCTTC TTCTGGTTCT TCTGGACCAA GCTGTTTAAC 
0481 GTCGTGGAGA ACTCCTACGG AC

Assemble Data

<PRE>

10671R-3.IR_full       1   TCCGCTGCGTCCAAATTTGGGCGGTACTGCGGGAAGTCCCTCCTCCTCCGGTTCCAACAT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10671R-3.in silico     1   TCCGCTGCGTCCAAATTTGGGCGGTACTGCGGGAAGTCCCTCCTCCTCCGGTTCCAACAT 60


10671R-3.IR_full       61  GAATTTCAGGCCCGGAGGACCTGACATCACCAGGTCGGAAGCGCGCGGAACGAGGCCCAC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10671R-3.in silico     61  GAATTTCAGGCCCGGAGGACCTGACATCACCAGGTCGGAAGCGCGCGGAACGAGGCCCAC 120


10671R-3.IR_full       121 GGCCGCACCCACTAGCAT<span class="snp"><tt>T</tt></span>CGCGAGATCCTGGTCATGGGCGTCATCCATTTGTGCAAGAA 180
                           ||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||
10671R-3.in silico     121 GGCCGCACCCACTAGCAT<span class="snp"><tt>C</tt></span>CGCGAGATCCTGGTCATGGGCGTCATCCATTTGTGCAAGAA 180


10671R-3.IR_full       181 GACCATATTCTTCAATACGGACCTCAAAGTAGCCTTGTATCTGGGCAGTCTGTTTGTGAT 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10671R-3.in silico     181 GACCATATTCTTCAATACGGACCTCAAAGTAGCCTTGTATCTGGGCAGTCTGTTTGTGAT 240


10671R-3.IR_full       241 CTCCGTAATCGGGGACTTTGTGCCCTTCCCCAAGACGTACTTCGCCCGATC<span class="snp"><tt>G</tt></span>GGACAACC 300
                           |||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||
10671R-3.in silico     241 CTCCGTAATCGGGGACTTTGTGCCCTTCCCCAAGACGTACTTCGCCCGATC<span class="snp"><tt>-</tt></span>GGACAACC 300


10671R-3.IR_full       301 TGTTCAACCAGTACTTCGTGAAGATCGGCTGGGGCTGGACGCTGCTCTTCGTGGTGCCCT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10671R-3.in silico     301 TGTTCAACCAGTACTTCGTGAAGATCGGCTGGGGCTGGACGCTGCTCTTCGTGGTGCCCT 360


10671R-3.IR_full       361 TCCTGGTGCTCTCGGCCTATACGATTACGTGTGGCGACCACAAACGGATGCTGCG<span class="snp"><tt>A</tt></span>ACAC 420
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||
10671R-3.in silico     361 TCCTGGTGCTCTCGGCCTATACGATTACGTGTGGCGACCACAAACGGATGCTGCG<span class="snp"><tt>-</tt></span>ACAC 420


10671R-3.IR_full       421 CATTTCCCGCGCATCGTCATAGCCACCTTCTTCTGGTTCTTCTGGACCAAGCTGTTTAA<span class="snp"><tt>T</tt></span> 480
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>
10671R-3.in silico     421 CATTTCCCGCGCATCGTCATAGCCACCTTCTTCTGGTTCTTCTGGACCAAGCTGTTTAA<span class="snp"><tt>C</tt></span> 480


10671R-3.IR_full       481 GTCGTGGAGAACTCCTACGGAC 502
                           ||||||||||||||||||||||
10671R-3.in silico     481 GTCGTGGAGAACTCCTACGGAC 502

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_168110.2	CG10671-RB, transcript variant B (CG10671), mRNA
100	482	0	0	0	NM_139687.2	CG10671-RA, transcript variant A (CG10671), mRNA
0.2	1	2	5	2	NM_169715.1	CG17931-RA, transcript variant A (CG17931), mRNA
0.2	1	2	5	2	NM_142280.1	CG17931-RB, transcript variant B (CG17931), mRNA
0	0	6	2	3	NM_139854.1	CG8583-RA (sec63), mRNA
0	0	3	3	2	NM_132888.1	CG13957-RA (CG13957), mRNA
0	0	3	2	7	NM_143388.1	CG14527-RA (CG14527), mRNA
0	0	2	4	2	NM_143453.1	CG7582-RA (CG7582), mRNA
0	0	2	3	2	NM_057737.2	CG15804-RA, transcript variant A (Dhc62B), mRNA
0	0	2	3	2	NM_206236.1	CG15804-RB, transcript variant B (Dhc62B), mRNA
0	0	2	2	5	NM_001038885.1	CG2803-RB, transcript variant B (Tina-1), mRNA
0	0	2	2	5	NM_138139.3	CG2803-RA, transcript variant A (Tina-1), mRNA
0	0	1	3	4	NM_141691.1	CG8500-RA (CG8500), mRNA
0	0	1	3	4	NM_164941.1	CG17118-RA (CG17118), mRNA
0	0	1	2	6	NM_141676.2	CG12950-RA (CG12950), mRNA
0	0	1	2	3	NM_165504.1	CG30160-RA (CG30160), mRNA
0	0	1	2	3	NM_165503.1	CG18816-RB, transcript variant B (Tsp42Eb), mRNA
0	0	1	2	3	NM_080272.2	CG18816-RA, transcript variant A (Tsp42Eb), mRNA
0	0	1	2	3	NM_142062.1	CG14363-RA (CG14363), mRNA
0	0	1	2	2	NM_057333.3	CG5711-RA (Arr1), mRNA
0	0	1	2	2	NM_167985.1	CG14955-RB, transcript variant B (CG14955), mRNA
0	0	1	2	2	NM_139512.1	CG14955-RA, transcript variant A (CG14955), mRNA
0	0	0	6	4	NM_140879.1	CG9392-RA (CG9392), mRNA
0	0	0	5	10	NM_165961.1	CG3845-RA, transcript variant A (l(2)01424), mRNA
0	0	0	5	10	NM_001038854.1	CG3845-RC, transcript variant C (l(2)01424), mRNA
0	0	0	5	10	NM_143770.2	CG3845-RB, transcript variant B (l(2)01424), mRNA
0	0	0	5	5	NM_130546.2	CG11409-RB (CG11409), mRNA
0	0	0	5	5	NM_142703.2	CG5911-RA, transcript variant A (ETHR), mRNA
0	0	0	5	5	NM_135287.1	CG13792-RA (CG13792), mRNA
0	0	0	5	4	NM_142874.2	CG6747-RA (Ir), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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