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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
10671R-1 |
| Symbol |
CG10671 |
Full Name |
CG10671 |
| CG No |
CG10671 |
Old CG No |
CG10671 |
| Synonyms |
i136, CG10671 |
| Accession No (Link to NCBI) |
NM_139687.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.<br> An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.<br> PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTCCGCTGCGTCCAAATTTGGG |
| Primer Seq. 3' |
aatctagaggtaccGTCCGTAGGAGTTCTCCACGA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 tCCGCTGCGT CCAAATTTGG GCGGTACTGC GGGAAGTCCC TCCTCCTCCG GTTCCAACAT
0061 GAATTTCAGG CCCGGAGGAC CTGACATCAC CAGGTCGGAA GCGCGCGGAA CGAGGCCCAC
0121 GGCCGCACCC ACTAGCATTC GCGAGATCCT GGTCATGGGC GTCATCCATT TGTGCAAGAA
0181 GACCATATTC TTCAATACGG ACCTCAAAGT AGCCTTGTAT CTGGGCAGTC TGTTTGTGAT
0241 CTCCGTAATC GGGGACTTTG TGCCCTTCCC CAAGACGTAC TTCGCCCGAT CGGGACAACC
0301 TGTTCAACCA GTACTTCGTG AAGATCGGCT GGGGCTGGAC GCTGCTCTTC GTGGTGCCCT
0361 TCCTGGTGCT CTCGGCCTAT ACGATTACGT GTGGCGACCA CAAACGGATG CTGCGAACAC
0421 CATTTCCCGC GCATCGTCAT AGCCACCTTC TTCTGGTTCT TCTGGACCAA GCTGTTTAAT
0481 GTCGTGGAGA ACTCCTACGG AC |
| in silico PCR Fragment |
0001 TCCGCTGCGT CCAAATTTGG GCGGTACTGC GGGAAGTCCC TCCTCCTCCG GTTCCAACAT
0061 GAATTTCAGG CCCGGAGGAC CTGACATCAC CAGGTCGGAA GCGCGCGGAA CGAGGCCCAC
0121 GGCCGCACCC ACTAGCATCC GCGAGATCCT GGTCATGGGC GTCATCCATT TGTGCAAGAA
0181 GACCATATTC TTCAATACGG ACCTCAAAGT AGCCTTGTAT CTGGGCAGTC TGTTTGTGAT
0241 CTCCGTAATC GGGGACTTTG TGCCCTTCCC CAAGACGTAC TTCGCCCGAT C-GGACAACC
0301 TGTTCAACCA GTACTTCGTG AAGATCGGCT GGGGCTGGAC GCTGCTCTTC GTGGTGCCCT
0361 TCCTGGTGCT CTCGGCCTAT ACGATTACGT GTGGCGACCA CAAACGGATG CTGCG-ACAC
0421 CATTTCCCGC GCATCGTCAT AGCCACCTTC TTCTGGTTCT TCTGGACCAA GCTGTTTAAC
0481 GTCGTGGAGA ACTCCTACGG AC |
| Assemble Data |
<PRE>
10671R-1.IR_full 1 TCCGCTGCGTCCAAATTTGGGCGGTACTGCGGGAAGTCCCTCCTCCTCCGGTTCCAACAT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10671R-1.in silico 1 TCCGCTGCGTCCAAATTTGGGCGGTACTGCGGGAAGTCCCTCCTCCTCCGGTTCCAACAT 60
10671R-1.IR_full 61 GAATTTCAGGCCCGGAGGACCTGACATCACCAGGTCGGAAGCGCGCGGAACGAGGCCCAC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10671R-1.in silico 61 GAATTTCAGGCCCGGAGGACCTGACATCACCAGGTCGGAAGCGCGCGGAACGAGGCCCAC 120
10671R-1.IR_full 121 GGCCGCACCCACTAGCAT<span class="snp"><tt>T</tt></span>CGCGAGATCCTGGTCATGGGCGTCATCCATTTGTGCAAGAA 180
||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||||||||
10671R-1.in silico 121 GGCCGCACCCACTAGCAT<span class="snp"><tt>C</tt></span>CGCGAGATCCTGGTCATGGGCGTCATCCATTTGTGCAAGAA 180
10671R-1.IR_full 181 GACCATATTCTTCAATACGGACCTCAAAGTAGCCTTGTATCTGGGCAGTCTGTTTGTGAT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10671R-1.in silico 181 GACCATATTCTTCAATACGGACCTCAAAGTAGCCTTGTATCTGGGCAGTCTGTTTGTGAT 240
10671R-1.IR_full 241 CTCCGTAATCGGGGACTTTGTGCCCTTCCCCAAGACGTACTTCGCCCGATC<span class="snp"><tt>G</tt></span>GGACAACC 300
|||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||
10671R-1.in silico 241 CTCCGTAATCGGGGACTTTGTGCCCTTCCCCAAGACGTACTTCGCCCGATC<span class="snp"><tt>-</tt></span>GGACAACC 300
10671R-1.IR_full 301 TGTTCAACCAGTACTTCGTGAAGATCGGCTGGGGCTGGACGCTGCTCTTCGTGGTGCCCT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10671R-1.in silico 301 TGTTCAACCAGTACTTCGTGAAGATCGGCTGGGGCTGGACGCTGCTCTTCGTGGTGCCCT 360
10671R-1.IR_full 361 TCCTGGTGCTCTCGGCCTATACGATTACGTGTGGCGACCACAAACGGATGCTGCG<span class="snp"><tt>A</tt></span>ACAC 420
|||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||
10671R-1.in silico 361 TCCTGGTGCTCTCGGCCTATACGATTACGTGTGGCGACCACAAACGGATGCTGCG<span class="snp"><tt>-</tt></span>ACAC 420
10671R-1.IR_full 421 CATTTCCCGCGCATCGTCATAGCCACCTTCTTCTGGTTCTTCTGGACCAAGCTGTTTAA<span class="snp"><tt>T</tt></span> 480
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>
10671R-1.in silico 421 CATTTCCCGCGCATCGTCATAGCCACCTTCTTCTGGTTCTTCTGGACCAAGCTGTTTAA<span class="snp"><tt>C</tt></span> 480
10671R-1.IR_full 481 GTCGTGGAGAACTCCTACGGAC 502
||||||||||||||||||||||
10671R-1.in silico 481 GTCGTGGAGAACTCCTACGGAC 502
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_168110.2 |
CG10671-RB, transcript variant B (CG10671), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_139687.2 |
CG10671-RA, transcript variant A (CG10671), mRNA |
| 0.2 |
1 |
2 |
5 |
2 |
NM_169715.1 |
CG17931-RA, transcript variant A (CG17931), mRNA |
| 0.2 |
1 |
2 |
5 |
2 |
NM_142280.1 |
CG17931-RB, transcript variant B (CG17931), mRNA |
| 0 |
0 |
6 |
2 |
3 |
NM_139854.1 |
CG8583-RA (sec63), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_132888.1 |
CG13957-RA (CG13957), mRNA |
| 0 |
0 |
3 |
2 |
7 |
NM_143388.1 |
CG14527-RA (CG14527), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_143453.1 |
CG7582-RA (CG7582), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_057737.2 |
CG15804-RA, transcript variant A (Dhc62B), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_206236.1 |
CG15804-RB, transcript variant B (Dhc62B), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_001038885.1 |
CG2803-RB, transcript variant B (Tina-1), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_138139.3 |
CG2803-RA, transcript variant A (Tina-1), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_141691.1 |
CG8500-RA (CG8500), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_164941.1 |
CG17118-RA (CG17118), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_141676.2 |
CG12950-RA (CG12950), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_165504.1 |
CG30160-RA (CG30160), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_165503.1 |
CG18816-RB, transcript variant B (Tsp42Eb), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_080272.2 |
CG18816-RA, transcript variant A (Tsp42Eb), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_142062.1 |
CG14363-RA (CG14363), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_057333.3 |
CG5711-RA (Arr1), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_167985.1 |
CG14955-RB, transcript variant B (CG14955), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_139512.1 |
CG14955-RA, transcript variant A (CG14955), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_140879.1 |
CG9392-RA (CG9392), mRNA |
| 0 |
0 |
0 |
5 |
10 |
NM_165961.1 |
CG3845-RA, transcript variant A (l(2)01424), mRNA |
| 0 |
0 |
0 |
5 |
10 |
NM_001038854.1 |
CG3845-RC, transcript variant C (l(2)01424), mRNA |
| 0 |
0 |
0 |
5 |
10 |
NM_143770.2 |
CG3845-RB, transcript variant B (l(2)01424), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_130546.2 |
CG11409-RB (CG11409), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_142703.2 |
CG5911-RA, transcript variant A (ETHR), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_135287.1 |
CG13792-RA (CG13792), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_142874.2 |
CG6747-RA (Ir), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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JNK signal
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Caspase-3 signal
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Pathways (updated: 2024/04/26)
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| KEGG Pathway |
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