NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

10667R-1

Symbol

Orc1

Full Name

Origin recognition complex subunit 1

CG No

CG10667

Old CG No

CG10667

Synonyms

ORC, rDmORC, DmORC1, ORC1, dORC1, CG10667, Orc1, DmOrc1p, orc1

Accession No (Link to NCBI)

NM_057955.3

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Cho Y, Lai CM, Lin KY, Hsu HJ. A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development. G3 (Bethesda) (2018) 8(7) 2345-2354 [ PubMed ID = 29764959 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgACGACACGCCAACAAACTGAA

Primer Seq. 3'

aatctagaggtaccCGCGATCCAGGCTGAGATTTA

Predicted Fragment Size

IR fragment full Seq

0001 ACGACACGCC AACAAACTGA AGTCCACGGC CTGCCCCATG TTTGTCAGCC GGTACAGGTT 
0061 TGTCAAGGTG AAGCGGagNT ANTTCGCTTG ATTCCCTTGG AAATTCACTT GGAGCAGCCG 
0121 GAGGACAATG CCAGGCCAAC CCGCTCATCT CGCAAATCCC TAACTGCACA CAGAGAATCC 
0181 AAGCGATCAA TATCCGCCCG CCACGATGAC ACAGCTGGCA ACAAGGGATC CTCAGTGGAA 
0241 AAACGACGAC GTGCGTCCAT GGCAGCCTCG AGTTCCGTGG AGTTCATCGA TGTCAACAGC 
0301 TTCATCTGCG AGAATAAAGT GTCGCCAATT AAGATCGTGG GCGGACGCAG TGTCGTCCGG 
0361 TTGTCTGAGA AGAAAAATGC TCCGGAGATC AATGCCAACT ATCTGCCGGC TTCGCCTCTC 
0421 ACTGAGAAGA ACGCCAAGGT GGAGACACCG AAAATCCCGG GCCTCGGCAG CCCGCCGCAA 
0481 CCTAAATCTC AGCCTGGATC GCG

in silico PCR Fragment

0001 ACGACACGCC AACAAACTGA AGTCCACGGC CTGCCCCATG TTTGTCAGCC GGTACAGGTT 
0061 TGTCAAGGTG AAGCGGAGCT A--TCGCTTG ATTCCCTTGG AAATTCACTT GGAGCAGCCG 
0121 GAGGACAATG CCAGGCCAAC CCGCTCATCT CGCAAATCCC TAACTGCACA CAGAGAATCC 
0181 AAGCGATCAA TATCCGCCCG CCACGATGAC ACAGCTGGCA ACAAGGGATC CTCAGTGGAA 
0241 AAACGACGAC GTGCGTCCAT GGCAGCCTCG AGTTCCGTGG AGTTCATCGA TGTCAACAGC 
0301 TTCATCTGCG AGAATAAAGT GTCGCCAATT AAGATCGTGG GCGGACGCAG TGTCGTCCGG 
0361 TTGTCTGAGA AGAAAAATGC TCCGGAGATC AATGCCAACT ATCTGCCGGC TTCGCCTCTC 
0421 ACTGAGAAGA ACGCCAAGGT GGAGACACCG -AAATCCCGG GCCTCGGCAG CCCGCCGCAA 
0481 CCTAAATCTC AGCCTGGATC GCG

Assemble Data

<PRE>

10667R-1.IR_full       1   ACGACACGCCAACAAACTGAAGTCCACGGCCTGCCCCATGTTTGTCAGCCGGTACAGGTT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10667R-1.in silico     1   ACGACACGCCAACAAACTGAAGTCCACGGCCTGCCCCATGTTTGTCAGCCGGTACAGGTT 60


10667R-1.IR_full       61  TGTCAAGGTGAAGCGGAG<span class="snp"><tt>N</tt></span>TA<span class="snp"><tt>N</tt></span><span class="snp"><tt>T</tt></span>TCGCTTGATTCCCTTGGAAATTCACTTGGAGCAGCCG 120
                           ||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||
10667R-1.in silico     61  TGTCAAGGTGAAGCGGAG<span class="snp"><tt>C</tt></span>TA<span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span>TCGCTTGATTCCCTTGGAAATTCACTTGGAGCAGCCG 120


10667R-1.IR_full       121 GAGGACAATGCCAGGCCAACCCGCTCATCTCGCAAATCCCTAACTGCACACAGAGAATCC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10667R-1.in silico     121 GAGGACAATGCCAGGCCAACCCGCTCATCTCGCAAATCCCTAACTGCACACAGAGAATCC 180


10667R-1.IR_full       181 AAGCGATCAATATCCGCCCGCCACGATGACACAGCTGGCAACAAGGGATCCTCAGTGGAA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10667R-1.in silico     181 AAGCGATCAATATCCGCCCGCCACGATGACACAGCTGGCAACAAGGGATCCTCAGTGGAA 240


10667R-1.IR_full       241 AAACGACGACGTGCGTCCATGGCAGCCTCGAGTTCCGTGGAGTTCATCGATGTCAACAGC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10667R-1.in silico     241 AAACGACGACGTGCGTCCATGGCAGCCTCGAGTTCCGTGGAGTTCATCGATGTCAACAGC 300


10667R-1.IR_full       301 TTCATCTGCGAGAATAAAGTGTCGCCAATTAAGATCGTGGGCGGACGCAGTGTCGTCCGG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10667R-1.in silico     301 TTCATCTGCGAGAATAAAGTGTCGCCAATTAAGATCGTGGGCGGACGCAGTGTCGTCCGG 360


10667R-1.IR_full       361 TTGTCTGAGAAGAAAAATGCTCCGGAGATCAATGCCAACTATCTGCCGGCTTCGCCTCTC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10667R-1.in silico     361 TTGTCTGAGAAGAAAAATGCTCCGGAGATCAATGCCAACTATCTGCCGGCTTCGCCTCTC 420


10667R-1.IR_full       421 ACTGAGAAGAACGCCAAGGTGGAGACACCG<span class="snp"><tt>A</tt></span>AAATCCCGGGCCTCGGCAGCCCGCCGCAA 480
                           ||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||
10667R-1.in silico     421 ACTGAGAAGAACGCCAAGGTGGAGACACCG<span class="snp"><tt>-</tt></span>AAATCCCGGGCCTCGGCAGCCCGCCGCAA 480


10667R-1.IR_full       481 CCTAAATCTCAGCCTGGATCGCG 503
                           |||||||||||||||||||||||
10667R-1.in silico     481 CCTAAATCTCAGCCTGGATCGCG 503

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_057955.3	CG10667-RA (Orc1), mRNA
0.2	1	2	2	2	NM_080259.1	CG14066-RA, transcript variant A (larp), mRNA
0.2	1	2	2	2	NM_170366.1	CG14066-RB, transcript variant B (larp), mRNA
0.2	1	2	2	2	NM_170365.1	CG14066-RC, transcript variant C (larp), mRNA
0	0	2	2	3	NM_138079.1	CG13582-RA (CG13582), mRNA
0	0	1	12	2	NM_164685.1	CG31642-RA (CG31642), mRNA
0	0	1	4	2	NM_135363.2	CG7806-RA (CG7806), mRNA
0	0	1	3	2	NM_142331.1	CG4009-RA (CG4009), mRNA
0	0	1	2	6	NM_141889.2	CG3169-RA (Spt3), mRNA
0	0	1	2	4	NM_079461.2	CG5219-RA (mRpL15), mRNA
0	0	1	2	4	NM_141855.2	CG6923-RA, transcript variant A (CG6923), mRNA
0	0	1	2	4	NM_169422.1	CG6923-RB, transcript variant B (CG6923), mRNA
0	0	1	2	3	NM_170044.2	CG4677-RB, transcript variant B (lmd), mRNA
0	0	1	2	3	NM_079733.3	CG4677-RA, transcript variant A (lmd), mRNA
0	0	1	2	2	NM_142439.1	CG7998-RA (CG7998), mRNA
0	0	1	2	2	NM_131979.1	CG5062-RA (CG5062), mRNA
0	0	0	8	3	NM_078900.2	CG12754-RA (Or42b), mRNA
0	0	0	6	3	NM_206294.1	CG33275-RA, transcript variant A (CG33275), mRNA
0	0	0	6	3	NM_137344.2	CG30456-RA (CG30456), mRNA
0	0	0	6	3	NM_206295.1	CG33275-RB, transcript variant B (CG33275), mRNA
0	0	0	6	3	NM_206293.1	CG33275-RC, transcript variant C (CG33275), mRNA
0	0	0	6	2	NM_136110.2	CG17564-RB (CG17564), mRNA
0	0	0	6	2	NM_142125.2	CG7825-RA (Rad17), mRNA
0	0	0	5	5	NM_001014610.1	CG33546-RB, transcript variant B (gfzf), mRNA
0	0	0	5	5	NM_132334.2	CG17252-RA (BCL7-like), mRNA
0	0	0	5	5	NM_079806.2	CG31062-RA (side), mRNA
0	0	0	5	2	NM_141572.1	CG11768-RA (CG11768), mRNA
0	0	0	4	6	NM_134516.2	CG32529-RA, transcript variant A (CG32529), mRNA
0	0	0	4	3	NM_136508.2	CG8711-RA (cul-4), mRNA
0	0	0	4	2	NM_143716.2	CG6754-RB (nbs), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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