NIG-Fly - Fly Stocks of National Institute of Genetics -

NIG-RNAi Strains Detail

Request : Click the "Order" button to request for this stock

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 10603R-4

Symbol mRpL13 Full Name mitochondrial ribosomal protein L13

CG No CG10603 Old CG No CG10603

Synonyms CG10603, L13, anon-EST:fe3C4, mRpL13

Accession No (Link to NCBI) NM_078874.2

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees pupal lethal

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATGTCCATTGCGAAGCGTGTG

Primer Seq. 3'

aatctagaggtaccTTGGGGAAGTTCTCCAGCGTT

Predicted Fragment Size

IR fragment full Seq

0001 ATGTCCATTG CGAAGCGTGT GCAGCAATGG GCAACGTTCG CGCGCACTTG GCACATTTAC 
0061 GATTGCACCT GGCAGAATCC ATTCGAGTCG GCAAAACTGG TTAAAACGCA TTTATTGGGC 
0121 CTCCAAAAGC CCATTTACCA CCCAATGAAT GACTGCGGGG ATCATGTGGT GCTGATCAAC 
0181 ACGCGGGAAA TCGCTTTGCC CGGTGACGAG TGGGTGAAGA GGGTTTACTT CCACCACACC 
0241 GGCTATCCTG GTGGCGCTTC GTGGACCCTG GCGTGGCAGC TGCACGAGAA GGATCCCACG 
0301 ATGGTGATGA AGAAGGCCGT GTACAACTCG ATGCGCGGAA ATCTGCAGCG CAGGCACACC 
0361 ATGCAAAGAC TGCACTTGTT CGCCGACGAT CAGGTGCCCG AGGAAATCCT GCAAAACGTC 
0421 ACGAATCAAA TCCGCNACGC CGCGCTCTAT TCCCCAGCGT CTGGATCATA TCGACAAAGA 
0481 AACGCTGGAG AACTTCCCCA A

in silico PCR Fragment

0001 ATGTCCATTG CGAAGCGTGT GCAGCAATGG GCAACGTTCG CGCGCACTTG GCACATTTAC 
0061 GATTGCACCT GGCAGAATCC ATTCGAGTCG GCAAAACTGG TTAAAACGCA TTTATTGGGC 
0121 CTCCAAAAGC CCATTTACCA CCCAATGAAT GACTGCGGGG ATCATGTGGT GCTGATCAAC 
0181 ACGCGGGAAA TCGCTTTGCC CGGTGACGAG TGGGTGAAGA GGGTTTACTT CCACCACACC 
0241 GGCTATCCTG GTGGCGCTTC GTGGACCCTG GCGTGGCAGC TGCACGAGAA GGATCCCACG 
0301 ATGGTGATGA AGAAGGCCGT GTACAACTCG ATGCGCGGAA ATCTGCAGCG CAGGCACACC 
0361 ATGCAAAGAC TGCACTTGTT CGCCGACGAT CAGGTGCCCG AGGAAATCCT GCAAAACGTC 
0421 ACGAATCAAA TCCGC-ACGC CGCGCTCTAT TCCCCAGCGT CTGGATCATA TCGACAAAGA 
0481 AACGCTGGAG AACTTCCCCA A

Assemble Data


10603R-4.IR_full       1   ATGTCCATTGCGAAGCGTGTGCAGCAATGGGCAACGTTCGCGCGCACTTGGCACATTTAC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10603R-4.in silico     1   ATGTCCATTGCGAAGCGTGTGCAGCAATGGGCAACGTTCGCGCGCACTTGGCACATTTAC 60


10603R-4.IR_full       61  GATTGCACCTGGCAGAATCCATTCGAGTCGGCAAAACTGGTTAAAACGCATTTATTGGGC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10603R-4.in silico     61  GATTGCACCTGGCAGAATCCATTCGAGTCGGCAAAACTGGTTAAAACGCATTTATTGGGC 120


10603R-4.IR_full       121 CTCCAAAAGCCCATTTACCACCCAATGAATGACTGCGGGGATCATGTGGTGCTGATCAAC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10603R-4.in silico     121 CTCCAAAAGCCCATTTACCACCCAATGAATGACTGCGGGGATCATGTGGTGCTGATCAAC 180


10603R-4.IR_full       181 ACGCGGGAAATCGCTTTGCCCGGTGACGAGTGGGTGAAGAGGGTTTACTTCCACCACACC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10603R-4.in silico     181 ACGCGGGAAATCGCTTTGCCCGGTGACGAGTGGGTGAAGAGGGTTTACTTCCACCACACC 240


10603R-4.IR_full       241 GGCTATCCTGGTGGCGCTTCGTGGACCCTGGCGTGGCAGCTGCACGAGAAGGATCCCACG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10603R-4.in silico     241 GGCTATCCTGGTGGCGCTTCGTGGACCCTGGCGTGGCAGCTGCACGAGAAGGATCCCACG 300


10603R-4.IR_full       301 ATGGTGATGAAGAAGGCCGTGTACAACTCGATGCGCGGAAATCTGCAGCGCAGGCACACC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10603R-4.in silico     301 ATGGTGATGAAGAAGGCCGTGTACAACTCGATGCGCGGAAATCTGCAGCGCAGGCACACC 360


10603R-4.IR_full       361 ATGCAAAGACTGCACTTGTTCGCCGACGATCAGGTGCCCGAGGAAATCCTGCAAAACGTC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10603R-4.in silico     361 ATGCAAAGACTGCACTTGTTCGCCGACGATCAGGTGCCCGAGGAAATCCTGCAAAACGTC 420


10603R-4.IR_full       421 ACGAATCAAATCCGCNACGCCGCGCTCTATTCCCCAGCGTCTGGATCATATCGACAAAGA 480
                           ||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||
10603R-4.in silico     421 ACGAATCAAATCCGC-ACGCCGCGCTCTATTCCCCAGCGTCTGGATCATATCGACAAAGA 480


10603R-4.IR_full       481 AACGCTGGAGAACTTCCCCAA 501
                           |||||||||||||||||||||
10603R-4.in silico     481 AACGCTGGAGAACTTCCCCAA 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_078874.2	CG10603-RA (mRpL13), mRNA
26.97	130	1	1	1	NM_136072.3	CG10602-RA, transcript variant A (CG10602), mRNA
0	0	4	2	3	NM_001014670.2	CG5462-RH, transcript variant H (scrib), mRNA
0	0	4	2	3	NM_170277.1	CG5462-RC, transcript variant C (scrib), mRNA
0	0	4	2	3	NM_001014669.1	CG5462-RI, transcript variant I (scrib), mRNA
0	0	4	2	3	NM_170275.1	CG5462-RA, transcript variant A (scrib), mRNA
0	0	4	2	3	NM_001043296.1	CG5462-RG, transcript variant G (scrib), mRNA
0	0	4	2	3	NM_170276.1	CG5462-RB, transcript variant B (scrib), mRNA
0	0	4	2	3	NM_080015.2	CG5462-RD, transcript variant D (scrib), mRNA
0	0	3	3	3	NM_136472.3	CG1399-RB, transcript variant B (CG1399), mRNA
0	0	3	2	2	NM_136371.2	CG9403-RA, transcript variant A (jing), mRNA
0	0	3	2	2	NM_206027.1	CG9403-RD, transcript variant D (jing), mRNA
0	0	3	2	2	NM_206026.1	CG9403-RC, transcript variant C (jing), mRNA
0	0	2	7	14	NM_144448.2	CG1915-RC, transcript variant C (sls), mRNA
0	0	2	2	2	NM_135917.2	CG18480-RA (CG18480), mRNA
0	0	2	1	1	NM_057962.4	CG1782-RA (Uba1), mRNA
0	0	1	10	5	NM_169938.1	CG5670-RC, transcript variant C (Atpalpha), mRNA
0	0	1	10	5	NM_206527.1	CG5670-RF, transcript variant F (Atpalpha), mRNA
0	0	1	10	5	NM_206526.1	CG5670-RG, transcript variant G (Atpalpha), mRNA
0	0	1	10	5	NM_169936.1	CG5670-RA, transcript variant A (Atpalpha), mRNA
0	0	1	10	5	NM_206528.1	CG5670-RE, transcript variant E (Atpalpha), mRNA
0	0	1	10	5	NM_169937.1	CG5670-RB, transcript variant B (Atpalpha), mRNA
0	0	1	10	5	NM_206525.1	CG5670-RH, transcript variant H (Atpalpha), mRNA
0	0	1	10	5	NM_169939.1	CG5670-RD, transcript variant D (Atpalpha), mRNA
0	0	1	6	6	NM_078670.2	CG7092-RA (Dhc16F), mRNA
0	0	1	5	8	NM_144472.2	CG1898-RA (HBS1), mRNA
0	0	1	3	8	NM_142215.2	CG5205-RA (CG5205), mRNA
0	0	1	2	4	NM_169525.1	CG18290-RB, transcript variant B (Act87E), mRNA
0	0	1	2	4	NM_135408.2	CG9466-RA (CG9466), mRNA
0	0	1	2	4	NM_057743.3	CG18290-RA, transcript variant A (Act87E), mRNA

Back