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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 10602R-2

Symbol CG10602 Full Name CG10602

CG No CG10602 Old CG No CG10602

Synonyms pepCG10602, CG10602

Accession No (Link to NCBI) NM_136072.3

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCGTCGATGATATTGGCCAGAA

Primer Seq. 3'

aatctagaggtaccAGACATACGGACCACACAACT

Predicted Fragment Size

IR fragment full Seq

0001 CGTCGATGAT ATTGGCCAGA AGCTAACGCT GGAGTTGCCA TCTGGAACGG CTAAGGGAAG 
0061 CCTTAACGTT CGCATCGATT ACGAGACTTC AAGCAGCGCC NGTGGGTTGC AGTGGCTGAA 
0121 TCCCACCCAG ACTTTGGGCA AGGAGCATCC CTATATGTTC TCCCAATGCC AGGCAATCCA 
0181 CGCTCGCTCG GTGATTCCCT GCCAAGACAC GCCAGCTGTT AAGTTTACCT ATGATGCCAC 
0241 AGTGGAGCAT CCCAGCGAGC TCACGGCCCT GATGAGCGCC CTTATCGATA AGAAGGAACC 
0301 GGGAAAGACA CTATTCAAGC AGGAGGTGCC CATTCCCGCT TATCTGGTGG CCATTGCCAT 
0361 TGGCAAATTG GTTTCCCGCC CGCTGGGGGA GAACTCCAGT GTCTGGGCTG AGGAAGCCAT 
0421 CGTGGATGCC TGCGCCGAGG AGTTTTCCGA AACAGCTACC ATGCTAAAGA CTGCCACTGA 
0481 GTTGTGTGGT CCGTATGTCT

in silico PCR Fragment

0001 CGTCGATGAT ATTGGCCAGA AGCTAACGCT GGAGTTGCCA TCTGGAACGG CTAAGGGAAG 
0061 CCTTAACGTT CGCATCGATT ACGAGACTTC AAGCAGCGCC AGTGGGTTGC AGTGGCTGAA 
0121 TCCCACCCAG ACTTTGGGCA AGGAGCATCC TTATATGTTC TCCCAATGCC AGGCAATCCA 
0181 CGCTCGCTCG GTGATTCCCT GCCAAGACAC GCCAGCTGTT AAGTTTACCT ATGATGCCAC 
0241 AGTGGAGCAT CCCAGCGAGC TCACGGCCCT GATGAGCGCC CTTATCGATA AGAAGGAACC 
0301 GGGAAAGACA CTATTCAAGC AGGAGGTGCC CATTCCCGCT TATCTGGTGG CCATTGCCAT 
0361 TGGCAAATTG GTTTCCCGCC CGCTGGGGGA GAACTCCAGT GTCTGGGCTG AGGAAGCCAT 
0421 CGTGGATGCC TGCGCCGAGG AGTTTTCCGA AACAGCTACC ATGCTAAAGA CTGCCACTGA 
0481 GTTGTGTGGT CCGTATGTCT

Assemble Data


10602R-2.IR_full       1   CGTCGATGATATTGGCCAGAAGCTAACGCTGGAGTTGCCATCTGGAACGGCTAAGGGAAG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10602R-2.in silico     1   CGTCGATGATATTGGCCAGAAGCTAACGCTGGAGTTGCCATCTGGAACGGCTAAGGGAAG 60


10602R-2.IR_full       61  CCTTAACGTTCGCATCGATTACGAGACTTCAAGCAGCGCCNGTGGGTTGCAGTGGCTGAA 120
                           |||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||
10602R-2.in silico     61  CCTTAACGTTCGCATCGATTACGAGACTTCAAGCAGCGCCAGTGGGTTGCAGTGGCTGAA 120


10602R-2.IR_full       121 TCCCACCCAGACTTTGGGCAAGGAGCATCCCTATATGTTCTCCCAATGCCAGGCAATCCA 180
                           |||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||
10602R-2.in silico     121 TCCCACCCAGACTTTGGGCAAGGAGCATCCTTATATGTTCTCCCAATGCCAGGCAATCCA 180


10602R-2.IR_full       181 CGCTCGCTCGGTGATTCCCTGCCAAGACACGCCAGCTGTTAAGTTTACCTATGATGCCAC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10602R-2.in silico     181 CGCTCGCTCGGTGATTCCCTGCCAAGACACGCCAGCTGTTAAGTTTACCTATGATGCCAC 240


10602R-2.IR_full       241 AGTGGAGCATCCCAGCGAGCTCACGGCCCTGATGAGCGCCCTTATCGATAAGAAGGAACC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10602R-2.in silico     241 AGTGGAGCATCCCAGCGAGCTCACGGCCCTGATGAGCGCCCTTATCGATAAGAAGGAACC 300


10602R-2.IR_full       301 GGGAAAGACACTATTCAAGCAGGAGGTGCCCATTCCCGCTTATCTGGTGGCCATTGCCAT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10602R-2.in silico     301 GGGAAAGACACTATTCAAGCAGGAGGTGCCCATTCCCGCTTATCTGGTGGCCATTGCCAT 360


10602R-2.IR_full       361 TGGCAAATTGGTTTCCCGCCCGCTGGGGGAGAACTCCAGTGTCTGGGCTGAGGAAGCCAT 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10602R-2.in silico     361 TGGCAAATTGGTTTCCCGCCCGCTGGGGGAGAACTCCAGTGTCTGGGCTGAGGAAGCCAT 420


10602R-2.IR_full       421 CGTGGATGCCTGCGCCGAGGAGTTTTCCGAAACAGCTACCATGCTAAAGACTGCCACTGA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10602R-2.in silico     421 CGTGGATGCCTGCGCCGAGGAGTTTTCCGAAACAGCTACCATGCTAAAGACTGCCACTGA 480


10602R-2.IR_full       481 GTTGTGTGGTCCGTATGTCT 500
                           ||||||||||||||||||||
10602R-2.in silico     481 GTTGTGTGGTCCGTATGTCT 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_136072.3	CG10602-RA, transcript variant A (CG10602), mRNA
100	482	0	0	0	NM_165269.1	CG10602-RD, transcript variant D (CG10602), mRNA
100	482	0	0	0	NM_165268.1	CG10602-RC, transcript variant C (CG10602), mRNA
100	482	0	0	0	NM_165267.1	CG10602-RB, transcript variant B (CG10602), mRNA
0	0	4	3	2	NM_135209.1	CG11320-RA (CG11320), mRNA
0	0	3	4	2	NM_057693.3	CG4799-RA (Pen), mRNA
0	0	3	3	5	NM_135382.1	CG13386-RA (CG13386), mRNA
0	0	2	5	3	NM_134829.2	CG4272-RA, transcript variant A (CG4272), mRNA
0	0	2	5	3	NM_164480.1	CG4272-RB, transcript variant B (CG4272), mRNA
0	0	2	2	2	NM_137865.1	CG13518-RA (Obp58b), mRNA
0	0	1	6	5	NM_057454.3	CG16720-RA, transcript variant A (5-HT1A), mRNA
0	0	1	6	5	NM_166322.1	CG16720-RB, transcript variant B (5-HT1A), mRNA
0	0	1	3	7	NM_165887.1	CG30047-RA (CG30047), mRNA
0	0	1	3	2	NM_142050.2	CG9347-RA (ninaB), mRNA
0	0	1	3	2	NM_057942.3	CG5227-RC, transcript variant C (sdk), mRNA
0	0	1	3	2	NM_134315.2	CG5227-RD, transcript variant D (sdk), mRNA
0	0	1	3	2	NM_134314.2	CG5227-RB, transcript variant B (sdk), mRNA
0	0	1	3	2	NM_057941.3	CG5227-RA, transcript variant A (sdk), mRNA
0	0	1	2	3	NM_142366.1	CG5863-RA (CG5863), mRNA
0	0	1	2	3	NM_078645.2	CG4252-RA (mei-41), mRNA
0	0	0	5	16	NM_132034.1	CG3108-RA (CG3108), mRNA
0	0	0	5	5	NM_167233.1	CG32687-RA (CG32687), mRNA
0	0	0	5	5	NM_139823.1	CG14825-RA (BBS1), mRNA
0	0	0	5	4	NM_078612.2	CG8470-RA (mRpS30), mRNA
0	0	0	5	3	NM_176156.1	CG30044-RA (CG30044), mRNA
0	0	0	4	6	NM_169499.1	CG8449-RA (CG8449), mRNA
0	0	0	4	4	NM_165538.1	CG1600-RA, transcript variant A (CG1600), mRNA
0	0	0	4	4	NM_165539.1	CG1600-RB, transcript variant B (CG1600), mRNA
0	0	0	4	4	NM_136449.2	CG1600-RC, transcript variant C (CG1600), mRNA
0	0	0	4	3	NM_140898.3	CG32217-RA (Su(Tpl)), mRNA

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