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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 10588R-1 
 Symbol CG10588  Full Name CG10588 
 CG No CG10588  Old CG No CG10588 
 Synonyms CG10588 
 Accession No (Link to NCBI) NM_141014.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgCATCGTGCGTCCAGAGAAAGT 
 Primer Seq. 3'
aatctagaggtaccCTATCATCGACGCCCTCTTTA 
 Predicted Fragment Size
500 
 IR fragment full Seq
0001 CATCGTGCGT CCAGAGAAAG TTTGAATTCC TCCACCGAGA ATTTTAATGG GAAATTGGCG 
0061 GCATGTGCAG TACTTGTTGG CGTTGGATCA TTTAGTGAAC CGCAACAATA TCAGGGATTA 
0121 GCACACTTTG TGGAACACAT GATATTTATG GGGTCTGAGA AATTCCCCGT AGAGAACGAA 
0181 TTCGATTCGT TTGTGACCAA AAGCGGCGGT TTCAGTAATG CGCATACTGA AAACGAGGAG 
0241 ACTTGCTTCT ACTTTGAGTT GGATCAAACT CACCTGGATA GGGGTATGGA TCTATTCATG 
0301 AACCTAATGA AGGCTCCACT AATGCTTCCC GATGCCATGA GTCGCGAACg CTCTGCCGTC 
0361 CAGTCGGAGT TCGAACAGAC CCATATGAGA GATGAGGTGC GGAGAGATCA GATCCTGGCG 
0421 AGCTTAGCCA GTGAGGGATA TCCACATGGC ACTTTCAGTT GGGGAAATTA TAAGACCCTT 
0481 AAAGAGGGCG TCGATGATAG  
 in silico PCR Fragment
0001 CATCGTGCGT CCAGAGAAAG TTTGAATTCC TCCACCGAGA ATTTTAATGG GAAATTGGCG 
0061 GCATGTGCAG TACTTGTTGG CGTTGGATCA TTTAGTGAAC CGCAACAATA TCAGGGATTA 
0121 GCACACTTTG TGGAACACAT GATATTTATG GGGTCTGAGA AATTCCCCGT AGAGAACGAA 
0181 TTCGATTCGT TTGTGACCAA AAGCGGCGGT TTCAGTAATG CGCATACTGA AAACGAGGAG 
0241 ACTTGCTTCT ACTTTGAGTT GGATCAAACT CACCTGGATA GGGGTATGGA TCTATTCATG 
0301 AACCTAATGA AGGCTCCACT AATGCTTCCC GATGCCATGA GTCGCGAACG CTCTGCCGTC 
0361 CAGTCGGAGT TCGAACAGAC CCATATGAGA GATGAGGTGC GGAGAGATCA GATCCTGGCG 
0421 AGCTTAGCCA GTGAGGGATA TCCACATGGC ACTTTCAGTT GGGGAAATTA TAAGACCCTT 
0481 AAAGAGGGCG TCGATGATAG  
 Assemble Data

10588R-1.IR_full       1   CATCGTGCGTCCAGAGAAAGTTTGAATTCCTCCACCGAGAATTTTAATGGGAAATTGGCG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10588R-1.in silico     1   CATCGTGCGTCCAGAGAAAGTTTGAATTCCTCCACCGAGAATTTTAATGGGAAATTGGCG 60


10588R-1.IR_full       61  GCATGTGCAGTACTTGTTGGCGTTGGATCATTTAGTGAACCGCAACAATATCAGGGATTA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10588R-1.in silico     61  GCATGTGCAGTACTTGTTGGCGTTGGATCATTTAGTGAACCGCAACAATATCAGGGATTA 120


10588R-1.IR_full       121 GCACACTTTGTGGAACACATGATATTTATGGGGTCTGAGAAATTCCCCGTAGAGAACGAA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10588R-1.in silico     121 GCACACTTTGTGGAACACATGATATTTATGGGGTCTGAGAAATTCCCCGTAGAGAACGAA 180


10588R-1.IR_full       181 TTCGATTCGTTTGTGACCAAAAGCGGCGGTTTCAGTAATGCGCATACTGAAAACGAGGAG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10588R-1.in silico     181 TTCGATTCGTTTGTGACCAAAAGCGGCGGTTTCAGTAATGCGCATACTGAAAACGAGGAG 240


10588R-1.IR_full       241 ACTTGCTTCTACTTTGAGTTGGATCAAACTCACCTGGATAGGGGTATGGATCTATTCATG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10588R-1.in silico     241 ACTTGCTTCTACTTTGAGTTGGATCAAACTCACCTGGATAGGGGTATGGATCTATTCATG 300


10588R-1.IR_full       301 AACCTAATGAAGGCTCCACTAATGCTTCCCGATGCCATGAGTCGCGAACGCTCTGCCGTC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10588R-1.in silico     301 AACCTAATGAAGGCTCCACTAATGCTTCCCGATGCCATGAGTCGCGAACGCTCTGCCGTC 360


10588R-1.IR_full       361 CAGTCGGAGTTCGAACAGACCCATATGAGAGATGAGGTGCGGAGAGATCAGATCCTGGCG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10588R-1.in silico     361 CAGTCGGAGTTCGAACAGACCCATATGAGAGATGAGGTGCGGAGAGATCAGATCCTGGCG 420


10588R-1.IR_full       421 AGCTTAGCCAGTGAGGGATATCCACATGGCACTTTCAGTTGGGGAAATTATAAGACCCTT 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10588R-1.in silico     421 AGCTTAGCCAGTGAGGGATATCCACATGGCACTTTCAGTTGGGGAAATTATAAGACCCTT 480


10588R-1.IR_full       481 AAAGAGGGCGTCGATGATAG 500
                           ||||||||||||||||||||
10588R-1.in silico     481 AAAGAGGGCGTCGATGATAG 500

 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  NM_141014.1  CG10588-RA (CG10588), mRNA 
0   NM_057981.3  CG4063-RA (ebi), mRNA 
0   NM_142335.2  CG5208-RA (CG5208), mRNA 
0   NM_001043313.1  CG34155-RA (CG34155), mRNA 
0   NM_166414.1  CG10067-RB, transcript variant B (Act57B), mRNA 
0   NM_079076.3  CG10067-RA, transcript variant A (Act57B), mRNA 
0   20  NM_136033.2  CG12750-RA (ncm), mRNA 
0   NM_135228.1  CG11321-RA, transcript variant A (CG11321), mRNA 
0   NM_164709.1  CG11321-RB, transcript variant B (CG11321), mRNA 
0   31  NM_132529.2  CG2025-RA (CG2025), mRNA 
0   NM_130646.2  CG14045-RA (CG14045), mRNA 
0   NM_079872.4  CG1873-RA, transcript variant A (Ef1alpha100E), mRNA 
0   NM_206592.1  CG1873-RD, transcript variant D (Ef1alpha100E), mRNA 
0   NM_206593.1  CG1873-RC, transcript variant C (Ef1alpha100E), mRNA 
0   NM_170570.1  CG1873-RB, transcript variant B (Ef1alpha100E), mRNA 
0   NM_144355.2  CG5442-RB, transcript variant B (SC35), mRNA 
0   NM_078717.2  CG3696-RA, transcript variant A (kis), mRNA 
0   NM_001043222.1  CG9610-RC (Poxm), mRNA 
0   NM_164918.1  CG5096-RA (CG5096), mRNA 
0   NM_079717.2  CG6703-RB, transcript variant B (Caki), mRNA 
0   NM_167955.1  CG1263-RB, transcript variant B (RpL8), mRNA 
0   NM_168466.2  CG6091-RB, transcript variant B (CG6091), mRNA 
0   NM_140225.3  CG6091-RA, transcript variant A (CG6091), mRNA 
0   NM_168465.2  CG6091-RC, transcript variant C (CG6091), mRNA 
0   NM_166452.1  CG9847-RB, transcript variant B (Fkbp13), mRNA 
0   NM_057625.3  CG9847-RA, transcript variant A (Fkbp13), mRNA 
0   NM_136955.2  CG8815-RA, transcript variant A (Sin3A), mRNA 
0   NM_165916.1  CG8815-RC, transcript variant C (Sin3A), mRNA 
0   NM_165915.1  CG8815-RB, transcript variant B (Sin3A), mRNA 
0   NM_165917.1  CG8815-RD, transcript variant D (Sin3A), mRNA 
 Wing Disc
GFP
Puc
Caspase
DAPI
Merge
Figure
JNK signal
N
 
DB
 
DC
+- 
DM
 
VM
 
VB
 
Caspase-3 signal
N
 
DB
 
DC
DM
 
VM
 
VB
 
Dcompartment   Penetrance
     

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