NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

10480R-1

Symbol

Bj1

Full Name

Bj1 protein

CG No

CG10480

Old CG No

CG10480

Synonyms

RanGEF, RCC1, CG18640 (Bj1), sdt, BJ1, inxs, CG10480, Bj1

Accession No (Link to NCBI)

NM_079219.2

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCGCAGAAAGGCCCTGACTAAT

Primer Seq. 3'

aatctagaggtaccCGCTTGTTACCATCGATGGTG

Predicted Fragment Size

IR fragment full Seq

0001 CGCAGAAAGG CCCTGACTAA TAACAACAAT GCCGGCGAAG cGGAGCAGCA GCCACCGAAN 
0061 GCGAAGCNCG CCAGAATCGC TTTTCATCTG GAGCTGCCCA AGCGGAGGAC CGTGCTCGGC 
0121 AACGTGCTAG TCTGCGGCAA CGGCGATGTG GGCCAACTTG GTCTCGGCGA GGACATACTG 
0181 GAGCGCAAGC GTCTCAGCCC TGTGGCGGGC ATCCCGGATG CCGTGGACAT ATNCGNCGGT 
0241 GGCATGCACA ACCTGGTGNT GACCAAGTCA GGGGACATTT ACTCATTCGG TTGCAATGAC 
0301 GAAGGTGCTC TAGGCCGTGA CACCAGCGAG GATGGCAGCG AGTCAANGCC GGATCTTATC 
0361 GATTTGCCCG GCAAGGCTCT GTGCATTTCG GCGGGAGACN CGCATTCCGC TTGTCTGCTG 
0421 GAGGATGGCC GTGTCTTCGC TTGGGGCTCG TTCCGGGACT CTCATGGCAA CATGGGCCTC 
0481 ACCATCGATG GTAACAAGCG

in silico PCR Fragment

0001 CGCAGAAAGG CCCTGACTAA TAACAACAAT GCCGGCGAAG CGGAGCAGCA GCCACCGAAG 
0061 GCGAAGCGCG CCAGAATCGC TTTTCATCTG GAGCTGCCCA AGCGGAGGAC CGTGCTCGGC 
0121 AACGTGCTAG TCTGCGGCAA CGGCGATGTG GGCCAACTTG GTCTCGGCGA GGACATACTG 
0181 GAGCGCAAGC GTCTCAGCCC TGTGGCGGGC ATCCCGGATG CCGTGGACAT ATCCGCCGGT 
0241 GGCATGCACA ACCTGGTGCT GACCAAGTCA GGGGACATTT ACTCATTCGG TTGCAATGAC 
0301 GAAGGTGCTC TAGGCCGTGA CACCAGCGAG GATGGCAGCG AGTCAAAGCC GGATCTTATC 
0361 GATTTGCCCG GCAAGGCTCT GTGCATTTCG GCGGGAGACT CGCATTCCGC TTGTCTGCTG 
0421 GAGGATGGCC GTGTCTTCGC TTGGGGCTCG TTCCGGGACT CTCATGGCAA CATGGGCCTC 
0481 ACCATCGATG GTAACAAGCG

Assemble Data

<PRE>

10480R-1.IR_full       1   CGCAGAAAGGCCCTGACTAATAACAACAATGCCGGCGAAGCGGAGCAGCAGCCACCGAA<span class="snp"><tt>N</tt></span> 60
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>
10480R-1.in silico     1   CGCAGAAAGGCCCTGACTAATAACAACAATGCCGGCGAAGCGGAGCAGCAGCCACCGAA<span class="snp"><tt>G</tt></span> 60


10480R-1.IR_full       61  GCGAAGC<span class="snp"><tt>N</tt></span>CGCCAGAATCGCTTTTCATCTGGAGCTGCCCAAGCGGAGGACCGTGCTCGGC 120
                           |||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||||||||||
10480R-1.in silico     61  GCGAAGC<span class="snp"><tt>G</tt></span>CGCCAGAATCGCTTTTCATCTGGAGCTGCCCAAGCGGAGGACCGTGCTCGGC 120


10480R-1.IR_full       121 AACGTGCTAGTCTGCGGCAACGGCGATGTGGGCCAACTTGGTCTCGGCGAGGACATACTG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10480R-1.in silico     121 AACGTGCTAGTCTGCGGCAACGGCGATGTGGGCCAACTTGGTCTCGGCGAGGACATACTG 180


10480R-1.IR_full       181 GAGCGCAAGCGTCTCAGCCCTGTGGCGGGCATCCCGGATGCCGTGGACATAT<span class="snp"><tt>N</tt></span>CG<span class="snp"><tt>N</tt></span>CGGT 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||<span class="snp"><tt>&nbsp;</tt></span>||||
10480R-1.in silico     181 GAGCGCAAGCGTCTCAGCCCTGTGGCGGGCATCCCGGATGCCGTGGACATAT<span class="snp"><tt>C</tt></span>CG<span class="snp"><tt>C</tt></span>CGGT 240


10480R-1.IR_full       241 GGCATGCACAACCTGGTG<span class="snp"><tt>N</tt></span>TGACCAAGTCAGGGGACATTTACTCATTCGGTTGCAATGAC 300
                           ||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||
10480R-1.in silico     241 GGCATGCACAACCTGGTG<span class="snp"><tt>C</tt></span>TGACCAAGTCAGGGGACATTTACTCATTCGGTTGCAATGAC 300


10480R-1.IR_full       301 GAAGGTGCTCTAGGCCGTGACACCAGCGAGGATGGCAGCGAGTCAA<span class="snp"><tt>N</tt></span>GCCGGATCTTATC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||
10480R-1.in silico     301 GAAGGTGCTCTAGGCCGTGACACCAGCGAGGATGGCAGCGAGTCAA<span class="snp"><tt>A</tt></span>GCCGGATCTTATC 360


10480R-1.IR_full       361 GATTTGCCCGGCAAGGCTCTGTGCATTTCGGCGGGAGAC<span class="snp"><tt>N</tt></span>CGCATTCCGCTTGTCTGCTG 420
                           |||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||
10480R-1.in silico     361 GATTTGCCCGGCAAGGCTCTGTGCATTTCGGCGGGAGAC<span class="snp"><tt>T</tt></span>CGCATTCCGCTTGTCTGCTG 420


10480R-1.IR_full       421 GAGGATGGCCGTGTCTTCGCTTGGGGCTCGTTCCGGGACTCTCATGGCAACATGGGCCTC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10480R-1.in silico     421 GAGGATGGCCGTGTCTTCGCTTGGGGCTCGTTCCGGGACTCTCATGGCAACATGGGCCTC 480


10480R-1.IR_full       481 ACCATCGATGGTAACAAGCG 500
                           ||||||||||||||||||||
10480R-1.in silico     481 ACCATCGATGGTAACAAGCG 500

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	1	NM_079219.2	CG10480-RA, transcript variant A (Bj1), mRNA
100	482	0	0	1	NM_168152.1	CG10480-RC, transcript variant C (Bj1), mRNA
100	482	0	0	1	NM_168151.1	CG10480-RB, transcript variant B (Bj1), mRNA
0.2	1	3	4	2	NM_137830.1	CG3701-RA (CG3701), mRNA
0	0	3	3	2	NM_132183.1	CG15324-RA (CG15324), mRNA
0	0	3	2	2	NM_143605.2	CG11339-RA (CG11339), mRNA
0	0	2	3	2	NM_169375.1	CG31299-RB, transcript variant B (nocturnin), mRNA
0	0	2	3	2	NM_169374.1	CG31299-RA, transcript variant A (nocturnin), mRNA
0	0	1	4	3	NM_078882.2	CG10757-RA, transcript variant A (mRpS18B), mRNA
0	0	1	4	3	NM_165321.1	CG10757-RB, transcript variant B (mRpS18B), mRNA
0	0	1	3	6	NM_167965.1	CG32296-RA (Mrtf), mRNA
0	0	1	3	4	NM_132310.2	CG2194-RB, transcript variant B (Reg-3), mRNA
0	0	1	3	4	NM_167182.1	CG2194-RC, transcript variant C (Reg-3), mRNA
0	0	1	3	4	NM_080323.2	CG10798-RA (dm), mRNA
0	0	1	3	3	NM_139993.1	CG13305-RA (CG13305), mRNA
0	0	1	3	2	NM_176679.2	CG4122-RC, transcript variant C (svr), mRNA
0	0	1	3	2	NM_206596.1	CG4122-RG, transcript variant G (svr), mRNA
0	0	1	3	2	NM_080293.3	CG4122-RB, transcript variant B (svr), mRNA
0	0	1	3	2	NM_206599.1	CG4122-RD, transcript variant D (svr), mRNA
0	0	1	3	2	NM_166846.3	CG4122-RA, transcript variant A (svr), mRNA
0	0	1	2	9	NM_143765.2	CG12218-RA (mei-P26), mRNA
0	0	1	2	4	NM_139447.1	CG2034-RA (CG2034), mRNA
0	0	1	2	3	NM_205934.1	CG13391-RB, transcript variant B (Aats-ala), mRNA
0	0	1	2	3	NM_078787.2	CG13391-RA, transcript variant A (Aats-ala), mRNA
0	0	1	2	2	NM_140466.1	CG5295-RA (CG5295), mRNA
0	0	1	2	2	NM_167176.1	CG7033-RB, transcript variant B (CG7033), mRNA
0	0	1	2	2	NM_132296.2	CG7033-RA, transcript variant A (CG7033), mRNA
0	0	1	2	2	NM_132871.2	CG8939-RA (CG8939), mRNA
0	0	1	2	2	NM_176715.1	CG7033-RC, transcript variant C (CG7033), mRNA
0	0	0	8	9	NM_139489.1	CG9977-RA (CG9977), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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