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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

10479R-3

Symbol

CG10479

Full Name

CG10479

CG No

CG10479

Old CG No

CG10479

Synonyms

CG10479

Accession No (Link to NCBI)

NM_139749.2

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

pupal lethal

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCAATCGCTTTGGCGCCACGGA

Primer Seq. 3'

aatctagaggtaccTCTGCCAGCAGTTGAGGGACA

Predicted Fragment Size

IR fragment full Seq

0001 CAATCGCTTT GGcGCCACGG ATGGCATCAA CAAAAACACC AGCAAAAAGG ATACTCAGCA 
0061 AAAGAACAAA ATGCTACNCA ATNGCCATCA AATGTCCTGG CTACTCGGAC ATCTATGGTC 
0121 AGTACAATAA CGGACTCGTC CTTCGAGAAG TGCGATGAAT GGAGCAACAA GCTGCAATTC 
0181 CCAGCCGCCG GAGAAATGGA TAAACAGGAA CCGATCCAGA GTCGCACGCA CAAGGTCTAC 
0241 GACAGCATGA AGAGTTTTCT GCAGCGCAAA CTCTTCGCGC AACCCTCGCA TAAAGAGCAG 
0301 ATCTTAAACG AAGAACCCAC AAGCGACTAT GATGAGNAGG ACCTTCTCGA CTCCTCGTAC 
0361 CACGNCGACG CGGAAGAAGA GGAGGAGGAA CNCGATGCGG ACTGCAGCTG CAGCTCCACC 
0421 GGCAGTAGCA GCACCAGCAG CAGCAGCCCA ACCACGCCGA AACGATCACC CCAAAAGTCC 
0481 CTGCTGTCCC TCAACTGCTG GCAGA

in silico PCR Fragment

0001 CAATCGCTTT GGCGCCACGG ATGGCATCAA CAAAAACACC AGCAAAAAGG ATACTCAGCA 
0061 AAAGAACAAA ATGCTACGCA AT-GCCATCA AATGTCCTGG CTACTCGGAC ATCTATGGTC 
0121 AGTACAATAA -GGACTCGTC CTTCGAGAAG TGCGATGAAT GGAGCAACAA GCTGCAATTC 
0181 CCAGCCGCCG GAGAAATGGA TAAACAGGAA CCGATCCAGA GTCGCACGCA CAAGGTCTAC 
0241 GACAGCATGA AGAGTTTTCT GCAGCGCAAA CTCTTCGCGC AACCCTCGCA TAAAGAGCAG 
0301 ATCTTAAACG AAGAACCCAC AAGCGACTAT GATG---AGG ACCTTCTCGA CTCCTCGTAC 
0361 CACGCCGACG CGGAAGAAGA GGAGGAGGAA CGCGATGCGG ACTGCAGCTG CAGCTCCACC 
0421 GGCAGTAGCA GCACCAGCAG CAGCAGCCCA ACCACGCCGA AACGATCACC CCAAAAGTCC 
0481 CTGCTGTCCC TCAACTGCTG GCAGA

Assemble Data

<PRE>

10479R-3.IR_full       1   CAATCGCTTTGGCGCCACGGATGGCATCAACAAAAACACCAGCAAAAAGGATACTCAGCA 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10479R-3.in silico     1   CAATCGCTTTGGCGCCACGGATGGCATCAACAAAAACACCAGCAAAAAGGATACTCAGCA 60


10479R-3.IR_full       61  AAAGAACAAAATGCTAC<span class="snp"><tt>N</tt></span>CAAT<span class="snp"><tt>N</tt></span>GCCATCAAATGTCCTGGCTACTCGGACATCTATGGTC 120
                           |||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||
10479R-3.in silico     61  AAAGAACAAAATGCTAC<span class="snp"><tt>G</tt></span>CAAT<span class="snp"><tt>-</tt></span>GCCATCAAATGTCCTGGCTACTCGGACATCTATGGTC 120


10479R-3.IR_full       121 AGTACAATAA<span class="snp"><tt>C</tt></span>GGACTCGTCCTTCGAGAAGTGCGATGAATGGAGCAACAAGCTGCAATTC 180
                           ||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||
10479R-3.in silico     121 AGTACAATAA<span class="snp"><tt>-</tt></span>GGACTCGTCCTTCGAGAAGTGCGATGAATGGAGCAACAAGCTGCAATTC 180


10479R-3.IR_full       181 CCAGCCGCCGGAGAAATGGATAAACAGGAACCGATCCAGAGTCGCACGCACAAGGTCTAC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10479R-3.in silico     181 CCAGCCGCCGGAGAAATGGATAAACAGGAACCGATCCAGAGTCGCACGCACAAGGTCTAC 240


10479R-3.IR_full       241 GACAGCATGAAGAGTTTTCTGCAGCGCAAACTCTTCGCGCAACCCTCGCATAAAGAGCAG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10479R-3.in silico     241 GACAGCATGAAGAGTTTTCTGCAGCGCAAACTCTTCGCGCAACCCTCGCATAAAGAGCAG 300


10479R-3.IR_full       301 ATCTTAAACGAAGAACCCACAAGCGACTATGATG<span class="snp"><tt>A</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>N</tt></span>AGGACCTTCTCGACTCCTCGTAC 360
                           ||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||
10479R-3.in silico     301 ATCTTAAACGAAGAACCCACAAGCGACTATGATG<span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span>AGGACCTTCTCGACTCCTCGTAC 360


10479R-3.IR_full       361 CACG<span class="snp"><tt>N</tt></span>CGACGCGGAAGAAGAGGAGGAGGAAC<span class="snp"><tt>N</tt></span>CGATGCGGACTGCAGCTGCAGCTCCACC 420
                           ||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||
10479R-3.in silico     361 CACG<span class="snp"><tt>C</tt></span>CGACGCGGAAGAAGAGGAGGAGGAAC<span class="snp"><tt>G</tt></span>CGATGCGGACTGCAGCTGCAGCTCCACC 420


10479R-3.IR_full       421 GGCAGTAGCAGCACCAGCAGCAGCAGCCCAACCACGCCGAAACGATCACCCCAAAAGTCC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10479R-3.in silico     421 GGCAGTAGCAGCACCAGCAGCAGCAGCCCAACCACGCCGAAACGATCACCCCAAAAGTCC 480


10479R-3.IR_full       481 CTGCTGTCCCTCAACTGCTGGCAGA 505
                           |||||||||||||||||||||||||
10479R-3.in silico     481 CTGCTGTCCCTCAACTGCTGGCAGA 505

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	12	NM_139749.2	CG10479-RA (CG10479), mRNA
0.62	3	21	77	226	NM_057771.2	CG1864-RB, transcript variant B (Hr38), mRNA
0.62	3	11	36	55	NM_132636.2	CG12723-RA (CG12723), mRNA
0.62	3	8	38	95	NM_080020.3	CG11491-RE, transcript variant E (br), mRNA
0.62	3	8	38	95	NM_166898.1	CG11491-RG, transcript variant G (br), mRNA
0.62	3	7	45	161	NM_001038740.1	CG32776-RD, transcript variant D (CG32776), mRNA
0.62	3	7	45	161	NM_206622.2	CG32776-RB, transcript variant B (CG32776), mRNA
0.62	3	6	35	127	NM_142259.2	CG10278-RA (GATAe), mRNA
0.62	3	6	31	95	NM_001038741.1	CG32776-RC, transcript variant C (CG32776), mRNA
0.62	3	6	31	95	NM_166996.2	CG32776-RA, transcript variant A (CG32776), mRNA
0.62	3	6	17	62	NM_136636.3	CG13739-RA (CG13739), mRNA
0.62	3	6	17	35	NM_169285.3	CG8144-RC, transcript variant C (ps), mRNA
0.62	3	6	17	35	NM_169284.2	CG8144-RD, transcript variant D (ps), mRNA
0.62	3	6	17	35	NM_001043326.1	CG8144-RI, transcript variant I (ps), mRNA
0.62	3	5	33	140	NM_080712.5	CG1693-RA, transcript variant A (tty), mRNA
0.62	3	5	33	140	NM_167744.3	CG1693-RB, transcript variant B (tty), mRNA
0.62	3	5	21	96	NM_057502.2	CG1322-RB, transcript variant B (zfh1), mRNA
0.62	3	5	21	58	NM_137930.2	CG3124-RA (CG3124), mRNA
0.62	3	5	18	91	NM_170522.2	CG1322-RC, transcript variant C (zfh1), mRNA
0.62	3	5	13	50	NM_170523.2	CG1322-RA, transcript variant A (zfh1), mRNA
0.62	3	4	24	90	NM_142180.2	CG4285-RA (CG4285), mRNA
0.62	3	4	17	49	NM_134555.1	CG15454-RA (CG15454), mRNA
0.62	3	3	7	54	NM_080325.3	CG6450-RC (lva), mRNA
0.62	3	3	6	26	NM_137583.3	CG11228-RA (hpo), mRNA
0.62	3	2	6	20	NM_132472.1	CG12625-RA (CG12625), mRNA
0.41	2	5	3	13	NM_134680.1	CG13691-RA (BBS8), mRNA
0.41	2	3	17	55	NM_057385.4	CG11121-RA (so), mRNA
0.41	2	3	4	12	NM_168153.1	CG32406-RA (CG32406), mRNA
0.41	2	2	37	141	NM_135793.2	CG16970-RA (CG16970), mRNA
0.41	2	2	5	31	NM_139967.1	CG7037-RB, transcript variant B (Cbl), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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