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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 10479R-2 
 Symbol CG10479  Full Name CG10479 
 CG No CG10479  Old CG No CG10479 
 Synonyms CG10479 
 Accession No (Link to NCBI) NM_139749.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication]
 
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgCAATCGCTTTGGCGCCACGGA 
 Primer Seq. 3'
aatctagaggtaccTCTGCCAGCAGTTGAGGGACA 
 Predicted Fragment Size
500 
 IR fragment full Seq
0001 CAATCGCTTT GGcGCCACGG ATGGCATCAA CAAAAACACC AGCAAAAAGG ATACTCAGCA 
0061 AAAGAACAAA ATGCTACNCA ATNGCCATCA AATGTCCTGG CTACTCGGAC ATCTATGGTC 
0121 AGTACAATAA CGGACTCGTC CTTCGAGAAG TGCGATGAAT GGAGCAACAA GCTGCAATTC 
0181 CCAGCCGCCG GAGAAATGGA TAAACAGGAA CCGATCCAGA GTCGCACGCA CAAGGTCTAC 
0241 GACAGCATGA AGAGTTTTCT GCAGCGCAAA CTCTTCGCGC AACCCTCGCA TAAAGAGCAG 
0301 ATCTTAAACG AAGAACCCAC AAGCGACTAT GATGAGNAGG ACCTTCTCGA CTCCTCGTAC 
0361 CACGNCGACG CGGAAGAAGA GGAGGAGGAA CNCGATGCGG ACTGCAGCTG CAGCTCCACC 
0421 GGCAGTAGCA GCACCAGCAG CAGCAGCCCA ACCACGCCGA AACGATCACC CCAAAAGTCC 
0481 CTGCTGTCCC TCAACTGCTG GCAGA 
 in silico PCR Fragment
0001 CAATCGCTTT GGCGCCACGG ATGGCATCAA CAAAAACACC AGCAAAAAGG ATACTCAGCA 
0061 AAAGAACAAA ATGCTACGCA AT-GCCATCA AATGTCCTGG CTACTCGGAC ATCTATGGTC 
0121 AGTACAATAA -GGACTCGTC CTTCGAGAAG TGCGATGAAT GGAGCAACAA GCTGCAATTC 
0181 CCAGCCGCCG GAGAAATGGA TAAACAGGAA CCGATCCAGA GTCGCACGCA CAAGGTCTAC 
0241 GACAGCATGA AGAGTTTTCT GCAGCGCAAA CTCTTCGCGC AACCCTCGCA TAAAGAGCAG 
0301 ATCTTAAACG AAGAACCCAC AAGCGACTAT GATG---AGG ACCTTCTCGA CTCCTCGTAC 
0361 CACGCCGACG CGGAAGAAGA GGAGGAGGAA CGCGATGCGG ACTGCAGCTG CAGCTCCACC 
0421 GGCAGTAGCA GCACCAGCAG CAGCAGCCCA ACCACGCCGA AACGATCACC CCAAAAGTCC 
0481 CTGCTGTCCC TCAACTGCTG GCAGA 
 Assemble Data
<PRE>

10479R-2.IR_full       1   CAATCGCTTTGGCGCCACGGATGGCATCAACAAAAACACCAGCAAAAAGGATACTCAGCA 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10479R-2.in silico     1   CAATCGCTTTGGCGCCACGGATGGCATCAACAAAAACACCAGCAAAAAGGATACTCAGCA 60


10479R-2.IR_full       61  AAAGAACAAAATGCTAC<span class="snp"><tt>N</tt></span>CAAT<span class="snp"><tt>N</tt></span>GCCATCAAATGTCCTGGCTACTCGGACATCTATGGTC 120
                           |||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||
10479R-2.in silico     61  AAAGAACAAAATGCTAC<span class="snp"><tt>G</tt></span>CAAT<span class="snp"><tt>-</tt></span>GCCATCAAATGTCCTGGCTACTCGGACATCTATGGTC 120


10479R-2.IR_full       121 AGTACAATAA<span class="snp"><tt>C</tt></span>GGACTCGTCCTTCGAGAAGTGCGATGAATGGAGCAACAAGCTGCAATTC 180
                           ||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||
10479R-2.in silico     121 AGTACAATAA<span class="snp"><tt>-</tt></span>GGACTCGTCCTTCGAGAAGTGCGATGAATGGAGCAACAAGCTGCAATTC 180


10479R-2.IR_full       181 CCAGCCGCCGGAGAAATGGATAAACAGGAACCGATCCAGAGTCGCACGCACAAGGTCTAC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10479R-2.in silico     181 CCAGCCGCCGGAGAAATGGATAAACAGGAACCGATCCAGAGTCGCACGCACAAGGTCTAC 240


10479R-2.IR_full       241 GACAGCATGAAGAGTTTTCTGCAGCGCAAACTCTTCGCGCAACCCTCGCATAAAGAGCAG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10479R-2.in silico     241 GACAGCATGAAGAGTTTTCTGCAGCGCAAACTCTTCGCGCAACCCTCGCATAAAGAGCAG 300


10479R-2.IR_full       301 ATCTTAAACGAAGAACCCACAAGCGACTATGATG<span class="snp"><tt>A</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>N</tt></span>AGGACCTTCTCGACTCCTCGTAC 360
                           ||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||
10479R-2.in silico     301 ATCTTAAACGAAGAACCCACAAGCGACTATGATG<span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span>AGGACCTTCTCGACTCCTCGTAC 360


10479R-2.IR_full       361 CACG<span class="snp"><tt>N</tt></span>CGACGCGGAAGAAGAGGAGGAGGAAC<span class="snp"><tt>N</tt></span>CGATGCGGACTGCAGCTGCAGCTCCACC 420
                           ||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||
10479R-2.in silico     361 CACG<span class="snp"><tt>C</tt></span>CGACGCGGAAGAAGAGGAGGAGGAAC<span class="snp"><tt>G</tt></span>CGATGCGGACTGCAGCTGCAGCTCCACC 420


10479R-2.IR_full       421 GGCAGTAGCAGCACCAGCAGCAGCAGCCCAACCACGCCGAAACGATCACCCCAAAAGTCC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10479R-2.in silico     421 GGCAGTAGCAGCACCAGCAGCAGCAGCCCAACCACGCCGAAACGATCACCCCAAAAGTCC 480


10479R-2.IR_full       481 CTGCTGTCCCTCAACTGCTGGCAGA 505
                           |||||||||||||||||||||||||
10479R-2.in silico     481 CTGCTGTCCCTCAACTGCTGGCAGA 505

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  12  NM_139749.2  CG10479-RA (CG10479), mRNA 
0.62   21  77  226  NM_057771.2  CG1864-RB, transcript variant B (Hr38), mRNA 
0.62   11  36  55  NM_132636.2  CG12723-RA (CG12723), mRNA 
0.62   38  95  NM_080020.3  CG11491-RE, transcript variant E (br), mRNA 
0.62   38  95  NM_166898.1  CG11491-RG, transcript variant G (br), mRNA 
0.62   45  161  NM_001038740.1  CG32776-RD, transcript variant D (CG32776), mRNA 
0.62   45  161  NM_206622.2  CG32776-RB, transcript variant B (CG32776), mRNA 
0.62   35  127  NM_142259.2  CG10278-RA (GATAe), mRNA 
0.62   31  95  NM_001038741.1  CG32776-RC, transcript variant C (CG32776), mRNA 
0.62   31  95  NM_166996.2  CG32776-RA, transcript variant A (CG32776), mRNA 
0.62   17  62  NM_136636.3  CG13739-RA (CG13739), mRNA 
0.62   17  35  NM_169285.3  CG8144-RC, transcript variant C (ps), mRNA 
0.62   17  35  NM_169284.2  CG8144-RD, transcript variant D (ps), mRNA 
0.62   17  35  NM_001043326.1  CG8144-RI, transcript variant I (ps), mRNA 
0.62   33  140  NM_080712.5  CG1693-RA, transcript variant A (tty), mRNA 
0.62   33  140  NM_167744.3  CG1693-RB, transcript variant B (tty), mRNA 
0.62   21  96  NM_057502.2  CG1322-RB, transcript variant B (zfh1), mRNA 
0.62   21  58  NM_137930.2  CG3124-RA (CG3124), mRNA 
0.62   18  91  NM_170522.2  CG1322-RC, transcript variant C (zfh1), mRNA 
0.62   13  50  NM_170523.2  CG1322-RA, transcript variant A (zfh1), mRNA 
0.62   24  90  NM_142180.2  CG4285-RA (CG4285), mRNA 
0.62   17  49  NM_134555.1  CG15454-RA (CG15454), mRNA 
0.62   54  NM_080325.3  CG6450-RC (lva), mRNA 
0.62   26  NM_137583.3  CG11228-RA (hpo), mRNA 
0.62   20  NM_132472.1  CG12625-RA (CG12625), mRNA 
0.41   13  NM_134680.1  CG13691-RA (BBS8), mRNA 
0.41   17  55  NM_057385.4  CG11121-RA (so), mRNA 
0.41   12  NM_168153.1  CG32406-RA (CG32406), mRNA 
0.41   37  141  NM_135793.2  CG16970-RA (CG16970), mRNA 
0.41   31  NM_139967.1  CG7037-RB, transcript variant B (Cbl), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway

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