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Notification of resumption of orders:

Orders have been suspended as a response to the COVID-19 infection, but it will be resumed today, May 11. However, we would like to set our organizational framework that prioritizes the maintenance of stocks for the time being. In addition, due to delays in delivery of postal items, it is expected that the flies you ordered will not reach you in normal period. We apologize for the inconvenience.

Thank you for your understanding.

NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 10463R-1 
 Symbol CG10463  Full Name CG10463 
 CG No CG10463  Old CG No CG10463 
 Synonyms CG10463 
 Accession No (Link to NCBI) NM_136156.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication]
 
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgCAAACGCGGACAGAACAAGAA 
 Primer Seq. 3'
aatctagaggtaccCTGCATCTAATATCTCGGTGG 
 Predicted Fragment Size
500 
 IR fragment full Seq
0001 CAAACGCGGA CAGAACAAGA ACCGCCCAGT TTTCAAGGAC GAGCGGTACT CGCATCTGTG 
0061 CCACTCGCTG ATCGATGGAA CCGGTGGAGA ACCTTGTTCC CTGGCCAACT GTCGCTATGT 
0121 CCACGACCTG GACGCCTACT TGGCCGCCAA GGGAGAAGAT TTGGGCCCTG AATGTTATGT 
0181 GTACACCACC AAGGGCTACT GTGCCCGCGG TGTGAGTTGT CGCTTTGCAA AGGCCCACAC 
0241 TGATGAACAG GGCAGGAACC TCAAAAGGGA GGACTACGAT GAAAAGGCTC CTCCAACCAC 
0301 ATGCAATGGC GTCAGTTCAG AACTCCAAGT GCGTCTACGA AAACATGAAT ATGACTTCAG 
0361 TCGGAGCAAG GAGCTTATTA AAATGGCTGA AAAGCTGAGG GATGCCCGAA AACTAATGGA 
0421 ACAACAGGAG AAGGAAAAGA ATCTGTCTGA AGAGACTGGA GAAAAACAAC CTAGTGAAAC 
0481 CACCGAGATA TTAGATGCAG  
 in silico PCR Fragment
0001 CAAACGCGGA CAGAACAAGA ACCGCCCAGT TTTCAAGGAC GAGCGGTACT CGCATCTGTG 
0061 CCACTCGCTG ATCGATGGAA CCGGTGGAGA ACCTTGTTCC CTGGCCAACT GTCGCTATGT 
0121 CCACGACCTG GACGCCTACT TGGCCGCCAA GGGAGAAGAT TTGGGCCCTG AATGTTATGT 
0181 GTACACCACC AAGGGCTACT GTGCCCGCGG TGTGAGTTGT CGCTTTGCAA AGGCCCACAC 
0241 TGATGAACAG GGCAGGAACC TCAAAAGGGA GGACTACGAT GAAAATGCTC CTCCAACCAC 
0301 ATGCAATGGC GTCAGTTCAG AACTCCAAGT GCGTCTACGA AAACATGAAT ATGACTTCAG 
0361 TCGGAGCAAG GAGCTTATTA AAATGGCTGA AAAGCTGAGG GATGCCCGAA AACTAAAGGA 
0421 ACAACAGGAG AAGGAAAAGA ATCCGTCTGA AGAGACTGGA GAAAAACAAC CTAGTGAAAC 
0481 CACCGAGATA TTAGATGCAG  
 Assemble Data

10463R-1.IR_full       1   CAAACGCGGACAGAACAAGAACCGCCCAGTTTTCAAGGACGAGCGGTACTCGCATCTGTG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10463R-1.in silico     1   CAAACGCGGACAGAACAAGAACCGCCCAGTTTTCAAGGACGAGCGGTACTCGCATCTGTG 60


10463R-1.IR_full       61  CCACTCGCTGATCGATGGAACCGGTGGAGAACCTTGTTCCCTGGCCAACTGTCGCTATGT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10463R-1.in silico     61  CCACTCGCTGATCGATGGAACCGGTGGAGAACCTTGTTCCCTGGCCAACTGTCGCTATGT 120


10463R-1.IR_full       121 CCACGACCTGGACGCCTACTTGGCCGCCAAGGGAGAAGATTTGGGCCCTGAATGTTATGT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10463R-1.in silico     121 CCACGACCTGGACGCCTACTTGGCCGCCAAGGGAGAAGATTTGGGCCCTGAATGTTATGT 180


10463R-1.IR_full       181 GTACACCACCAAGGGCTACTGTGCCCGCGGTGTGAGTTGTCGCTTTGCAAAGGCCCACAC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10463R-1.in silico     181 GTACACCACCAAGGGCTACTGTGCCCGCGGTGTGAGTTGTCGCTTTGCAAAGGCCCACAC 240


10463R-1.IR_full       241 TGATGAACAGGGCAGGAACCTCAAAAGGGAGGACTACGATGAAAAGGCTCCTCCAACCAC 300
                           ||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||
10463R-1.in silico     241 TGATGAACAGGGCAGGAACCTCAAAAGGGAGGACTACGATGAAAATGCTCCTCCAACCAC 300


10463R-1.IR_full       301 ATGCAATGGCGTCAGTTCAGAACTCCAAGTGCGTCTACGAAAACATGAATATGACTTCAG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10463R-1.in silico     301 ATGCAATGGCGTCAGTTCAGAACTCCAAGTGCGTCTACGAAAACATGAATATGACTTCAG 360


10463R-1.IR_full       361 TCGGAGCAAGGAGCTTATTAAAATGGCTGAAAAGCTGAGGGATGCCCGAAAACTAATGGA 420
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||
10463R-1.in silico     361 TCGGAGCAAGGAGCTTATTAAAATGGCTGAAAAGCTGAGGGATGCCCGAAAACTAAAGGA 420


10463R-1.IR_full       421 ACAACAGGAGAAGGAAAAGAATCTGTCTGAAGAGACTGGAGAAAAACAACCTAGTGAAAC 480
                           ||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||
10463R-1.in silico     421 ACAACAGGAGAAGGAAAAGAATCCGTCTGAAGAGACTGGAGAAAAACAACCTAGTGAAAC 480


10463R-1.IR_full       481 CACCGAGATATTAGATGCAG 500
                           ||||||||||||||||||||
10463R-1.in silico     481 CACCGAGATATTAGATGCAG 500

 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  NM_136156.1  CG10463-RA (CG10463), mRNA 
0   NM_135671.1  CG12602-RA (CG12602), mRNA 
0   NM_079403.3  CG6292-RB, transcript variant B (CycT), mRNA 
0   NM_001015497.1  CG40448-PA.3 (CG40448), mRNA 
0   NM_143320.2  CG18437-RA (CG18437), mRNA 
0   NM_175925.1  CG1651-RA, transcript variant A (Ank), mRNA 
0   NM_175926.1  CG1651-RB, transcript variant B (Ank), mRNA 
0   NM_175928.1  CG1651-RD, transcript variant D (Ank), mRNA 
0   NM_175927.1  CG1651-RC, transcript variant C (Ank), mRNA 
0   NM_140427.1  CG6513-RA, transcript variant A (endos), mRNA 
0   NM_168572.1  CG6513-RB, transcript variant B (endos), mRNA 
0   NM_078796.3  CG13106-RA (Or30a), mRNA 
0   NM_138138.3  CG2811-RA (CG2811), mRNA 
0   NM_080326.4  CG4944-RB, transcript variant B (cib), mRNA 
0   NM_167001.1  CG4944-RA, transcript variant A (cib), mRNA 
0   NM_057242.3  CG7765-RA (Khc), mRNA 
0   NM_167506.1  CG18734-RA, transcript variant A (Fur2), mRNA 
0   NM_078644.2  CG18734-RD, transcript variant D (Fur2), mRNA 
0   NM_206763.1  CG18734-RG, transcript variant G (Fur2), mRNA 
0   NM_167508.1  CG18734-RC, transcript variant C (Fur2), mRNA 
0   NM_167510.1  CG18734-RF, transcript variant F (Fur2), mRNA 
0   NM_167507.1  CG18734-RB, transcript variant B (Fur2), mRNA 
0   NM_167509.1  CG18734-RE, transcript variant E (Fur2), mRNA 
0   NM_168492.1  CG32099-RA (eap), mRNA 
0   NM_078651.2  CG18582-RA (mbt), mRNA 
0   NM_140026.2  CG5133-RA (Doc1), mRNA 
0   NM_001038886.1  CG34038-RA (CG34038), mRNA 
0   NM_132114.1  CG14442-RA (CG14442), mRNA 
0   NM_139981.2  CG6831-RA (rhea), mRNA 
0   NM_141509.2  CG7900-RA (CG7900), mRNA 

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