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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 10419R-3 
 Symbol CG10419  Full Name CG10419 
 CG No CG10419  Old CG No CG10419 
 Synonyms CG10419 
 Accession No (Link to NCBI) NM_140835.2 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees pupal lethal 
 Map Viewer
 Reference
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 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgATGCAGCATGAGCCCGAAGAT 
 Primer Seq. 3' 
aatctagaggtaccGTGTTGGGATCCTGCAGCCAC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 ATGCAGCATG AGCCCGAAGA TCAGACTTTT CAACTCCAGG CACTGGAGTA TCTGTGAGCC 
0061 GGATAGCAGT TTTGACCCCC AGAAGCCGCC AGAGTCCGGG GAAGAGTACC TAATGCACAT 
0121 GTTCTACGAG AGGAAGCGAT GTCCAGCTGT GGTGACCAAA AGATCATCCA AAATCAGGAA 
0181 TAACACGGGA AATACCACTT TAGAGATGTT GGACAATCCC GAGTTGCCTC CGTTCAAGTG 
0241 CCTGCTGCCC ACGCCCGAAT GGCGGGATGA GCAAGTTAAA TCCTTCCAAG CAGCCCGCTC 
0301 CCAGGTCCTA GTTCTTCGTA AGGAGCTGGC CAACAACAAC TACGACCAGT CCGGGGAACC 
0361 GCCTTTGACT TCCGACCAGG AGAAATGGAA GGAATTCTGC CGGAATCAGC AGCCCCTCCT 
0421 GAGCACCCTG CTCCATTTGA CCCAAAACGA CTTGGAGCTC TTGCTGGAAA TGCTCAGCAA 
0481 GTGGCTGCAG GATCCCAACA C 
 in silico PCR Fragment 
0001 ATGCAGCATG AGCCCGAAGA TCAGACTTTT CAACTCCAGG CACTGGAG-A TCTGTGAGCC 
0061 GGATAGCAGT TTTGACCCCC AGAAGCCGCC AGAGTCCGGG GAAGAGTACC TAATGCACAT 
0121 GTTCTACGAG AGGAAGCGAT GTCCAGCTGT GGTGACCAAA AGATCATCCA AAATCAGGAA 
0181 TAACACGGGA AATACCACTT TAGAGATGTT GGACAATCCC GAGTTGCCTC CGTTCAAGTG 
0241 CCTGCTGCCC ACGCCCGAAT GGCGGGATGA GCAAGTTAAA TCCTTCCAAG CAGCCCGCTC 
0301 CCAGGTCCTA GTTCTTCGTA AGGAGTTGGC CAACAACAAC TACGACCAGT CCGGGGAACC 
0361 ACCTTTGACT TCCGACCAGG AGAAATGGAA GGAATTCTGC CGGAATCAGC AGCCCCTCCT 
0421 GAGCACCCTG CTCCATTTGA CCCAAAACGA CTTGGAGCTC TTGCTGGAAA TGCTCAGCAA 
0481 GTGGCTGCAG GATCCCAACA C 
 Assemble Data 
<PRE>

10419R-3.IR_full       1   ATGCAGCATGAGCCCGAAGATCAGACTTTTCAACTCCAGGCACTGGAG<span class="snp"><tt>T</tt></span>ATCTGTGAGCC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||
10419R-3.in silico     1   ATGCAGCATGAGCCCGAAGATCAGACTTTTCAACTCCAGGCACTGGAG<span class="snp"><tt>-</tt></span>ATCTGTGAGCC 60


10419R-3.IR_full       61  GGATAGCAGTTTTGACCCCCAGAAGCCGCCAGAGTCCGGGGAAGAGTACCTAATGCACAT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10419R-3.in silico     61  GGATAGCAGTTTTGACCCCCAGAAGCCGCCAGAGTCCGGGGAAGAGTACCTAATGCACAT 120


10419R-3.IR_full       121 GTTCTACGAGAGGAAGCGATGTCCAGCTGTGGTGACCAAAAGATCATCCAAAATCAGGAA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10419R-3.in silico     121 GTTCTACGAGAGGAAGCGATGTCCAGCTGTGGTGACCAAAAGATCATCCAAAATCAGGAA 180


10419R-3.IR_full       181 TAACACGGGAAATACCACTTTAGAGATGTTGGACAATCCCGAGTTGCCTCCGTTCAAGTG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10419R-3.in silico     181 TAACACGGGAAATACCACTTTAGAGATGTTGGACAATCCCGAGTTGCCTCCGTTCAAGTG 240


10419R-3.IR_full       241 CCTGCTGCCCACGCCCGAATGGCGGGATGAGCAAGTTAAATCCTTCCAAGCAGCCCGCTC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10419R-3.in silico     241 CCTGCTGCCCACGCCCGAATGGCGGGATGAGCAAGTTAAATCCTTCCAAGCAGCCCGCTC 300


10419R-3.IR_full       301 CCAGGTCCTAGTTCTTCGTAAGGAG<span class="snp"><tt>C</tt></span>TGGCCAACAACAACTACGACCAGTCCGGGGAACC 360
                           |||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||
10419R-3.in silico     301 CCAGGTCCTAGTTCTTCGTAAGGAG<span class="snp"><tt>T</tt></span>TGGCCAACAACAACTACGACCAGTCCGGGGAACC 360


10419R-3.IR_full       361 <span class="snp"><tt>G</tt></span>CCTTTGACTTCCGACCAGGAGAAATGGAAGGAATTCTGCCGGAATCAGCAGCCCCTCCT 420
                           <span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10419R-3.in silico     361 <span class="snp"><tt>A</tt></span>CCTTTGACTTCCGACCAGGAGAAATGGAAGGAATTCTGCCGGAATCAGCAGCCCCTCCT 420


10419R-3.IR_full       421 GAGCACCCTGCTCCATTTGACCCAAAACGACTTGGAGCTCTTGCTGGAAATGCTCAGCAA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10419R-3.in silico     421 GAGCACCCTGCTCCATTTGACCCAAAACGACTTGGAGCTCTTGCTGGAAATGCTCAGCAA 480


10419R-3.IR_full       481 GTGGCTGCAGGATCCCAACAC 501
                           |||||||||||||||||||||
10419R-3.in silico     481 GTGGCTGCAGGATCCCAACAC 501

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_140835.2  CG10419-RA (CG10419), mRNA 
0   0  5  2  2  NM_164563.2  CG3980-RA, transcript variant A (CG3980), mRNA 
0   0  5  2  2  NM_134967.2  CG3980-RB, transcript variant B (CG3980), mRNA 
0   0  3  2  4  NM_140582.2  CG5215-RA, transcript variant A (Zn72D), mRNA 
0   0  3  2  4  NM_176332.1  CG5215-RB, transcript variant B (Zn72D), mRNA 
0   0  2  4  6  NM_142815.2  CG6949-RA (mRpL45), mRNA 
0   0  2  4  2  NM_132155.2  CG4617-RA (CG4617), mRNA 
0   0  2  2  2  NM_132014.2  CG4119-RA (CG4119), mRNA 
0   0  2  2  2  NM_206244.1  CG33232-RC, transcript variant C (CG33232), mRNA 
0   0  2  2  2  NM_206246.1  CG33232-RA, transcript variant A (CG33232), mRNA 
0   0  2  2  2  NM_001031933.1  CG33232-RD, transcript variant D (CG33232), mRNA 
0   0  2  2  2  NM_206245.1  CG33232-RB, transcript variant B (CG33232), mRNA 
0   0  1  5  4  NM_135125.1  CG14000-RA (CG14000), mRNA 
0   0  1  4  7  NM_136593.1  CG8181-RA (CG8181), mRNA 
0   0  1  4  3  NM_132956.1  CG9086-RA (CG9086), mRNA 
0   0  1  2  5  NM_135412.1  CG9487-RA (CG9487), mRNA 
0   0  1  2  4  NM_130606.2  CG3573-RA (CG3573), mRNA 
0   0  1  2  4  NM_139361.1  CG12090-RB, transcript variant B (CG12090), mRNA 
0   0  1  2  4  NM_167888.1  CG12090-RC, transcript variant C (CG12090), mRNA 
0   0  1  2  4  NM_167889.1  CG12090-RA, transcript variant A (CG12090), mRNA 
0   0  1  2  4  NM_167188.1  CG32703-RA (CG32703), mRNA 
0   0  1  2  2  NM_164853.1  CG3747-RB, transcript variant B (Eaat1), mRNA 
0   0  1  2  2  NM_058080.2  CG3747-RA, transcript variant A (Eaat1), mRNA 
0   0  1  2  2  NM_164854.1  CG3747-RC, transcript variant C (Eaat1), mRNA 
0   0  0  34  193  NM_078751.3  CG3047-RA (Sgs1), mRNA 
0   0  0  9  2  NM_078926.3  CG1712-RA, transcript variant A (Gr43a), mRNA 
0   0  0  9  2  NM_001043066.1  CG1712-RB, transcript variant B (Gr43a), mRNA 
0   0  0  6  21  NM_206332.1  CG18331-RA (CG18331), mRNA 
0   0  0  6  6  NM_001015169.1  CG41063-PA.3 (CG41063), mRNA 
0   0  0  5  4  NM_132009.2  CG11473-RA (CG11473), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway

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