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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 10366R-4 
 Symbol CG10366  Full Name CG10366 
 CG No CG10366  Old CG No CG10366 
 Synonyms CG10366 
 Accession No (Link to NCBI) NM_136151.1 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees male semi-lethal 
 Map Viewer
 Reference
[Please submit your publication]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS ONE (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS ONE (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgAGGTGGAGATGCACGCTGCTC 
 Primer Seq. 3'
aatctagaggtaccCTGCTTTTCCTCTGGCGTTAG 
 Predicted Fragment Size
500 
 IR fragment full Seq
0001 AGGTGGAGAT GCACGCTGCT CCGGCGGCTC CCCATGTCGA TCGAAATCCC AGCAGCGACT 
0061 GGCGACAGTG GTGTCGCCTG TGCGCCAAGG ACGACGTGCA AGGCAACTTG AATGTGTTCC 
0121 TCAAGAACGA GGATTCCGGC GAGGCAGCCT CCATGGANAC TGGCCACCGN CATTGGCAAG 
0181 TACTTCTGGG TGAATATAAC TCGAGGTGAA GAGCTGCCGG AGACGATATG CAGTGAGTGC 
0241 CTGTCGCTGG TCACCTCGCT GGTGAATTTC TCGGAACGGA TTACCCGCGT GCAGAAGCTT 
0301 TACTGCATCC TGCAGAGCAC GCCCAGCCAG GAGAGGCCAA ATNCTCCACG AGCTGCGCTC 
0361 GAAATTTGGT TTGCTGGACG AGGAGACGCA GACTGAGGTG CACTATGTGG ACAAGAAGCC 
0421 GAAGTTGGAT GAACAGCTCG AGTTTCTGCC AGAGGAGTCG CCTTACGAGC TGGCAAACCC 
0481 ACTAACGCCA GAGGAAAATG CAG 
 in silico PCR Fragment
0001 AGGTGGAGAT GCACGCTGCT CCGGCGGCTC CCCATGTCGA TCGAAATCCC AGCAGCGACT 
0061 GGCGACAGTG GTGTCGCCTG TGCGCCAAGG ACGACGTGCA AGGCAACTTG AATGTGTTCC 
0121 TCAAGAACGA GGATTCCGGC GAGGCAGCCT CCATGGA-AC TGGCCACCGC CATTGGCAAG 
0181 TACTTCTGGG TGAATATAAC TCGAGGTGAA GAGCTGCCGG AGACGATATG CAGTGAGTGC 
0241 CTGTCGCTGG TCACCTCGCT GGTGAATTTC TCGGAACGGA TTACCCGCGT GCAGAAGCTT 
0301 TACTGCATCC TGCAGAGCAC GCCCAGCCAG GAGAGGCCAA AT-CTCCACG AGCTGCGCTC 
0361 GAAATTTGGT TTGCTGGACG AGGAGACGCA GACTGAGGTG CACTATGTGG ACAAGAAGCC 
0421 GAAGTTGGAT GAACAGCTCG AGTTTCTGCC AGAGGAGTCG CCTTACGAGC TGGCAAACCC 
0481 ACTAACGCCA GAGGAAAA-G CAG 
 Assemble Data

10366R-4.IR_full       1   AGGTGGAGATGCACGCTGCTCCGGCGGCTCCCCATGTCGATCGAAATCCCAGCAGCGACT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10366R-4.in silico     1   AGGTGGAGATGCACGCTGCTCCGGCGGCTCCCCATGTCGATCGAAATCCCAGCAGCGACT 60


10366R-4.IR_full       61  GGCGACAGTGGTGTCGCCTGTGCGCCAAGGACGACGTGCAAGGCAACTTGAATGTGTTCC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10366R-4.in silico     61  GGCGACAGTGGTGTCGCCTGTGCGCCAAGGACGACGTGCAAGGCAACTTGAATGTGTTCC 120


10366R-4.IR_full       121 TCAAGAACGAGGATTCCGGCGAGGCAGCCTCCATGGANACTGGCCACCGNCATTGGCAAG 180
                           ||||||||||||||||||||||||||||||||||||| ||||||||||| ||||||||||
10366R-4.in silico     121 TCAAGAACGAGGATTCCGGCGAGGCAGCCTCCATGGA-ACTGGCCACCGCCATTGGCAAG 180


10366R-4.IR_full       181 TACTTCTGGGTGAATATAACTCGAGGTGAAGAGCTGCCGGAGACGATATGCAGTGAGTGC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10366R-4.in silico     181 TACTTCTGGGTGAATATAACTCGAGGTGAAGAGCTGCCGGAGACGATATGCAGTGAGTGC 240


10366R-4.IR_full       241 CTGTCGCTGGTCACCTCGCTGGTGAATTTCTCGGAACGGATTACCCGCGTGCAGAAGCTT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10366R-4.in silico     241 CTGTCGCTGGTCACCTCGCTGGTGAATTTCTCGGAACGGATTACCCGCGTGCAGAAGCTT 300


10366R-4.IR_full       301 TACTGCATCCTGCAGAGCACGCCCAGCCAGGAGAGGCCAAATNCTCCACGAGCTGCGCTC 360
                           |||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||
10366R-4.in silico     301 TACTGCATCCTGCAGAGCACGCCCAGCCAGGAGAGGCCAAAT-CTCCACGAGCTGCGCTC 360


10366R-4.IR_full       361 GAAATTTGGTTTGCTGGACGAGGAGACGCAGACTGAGGTGCACTATGTGGACAAGAAGCC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10366R-4.in silico     361 GAAATTTGGTTTGCTGGACGAGGAGACGCAGACTGAGGTGCACTATGTGGACAAGAAGCC 420


10366R-4.IR_full       421 GAAGTTGGATGAACAGCTCGAGTTTCTGCCAGAGGAGTCGCCTTACGAGCTGGCAAACCC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10366R-4.in silico     421 GAAGTTGGATGAACAGCTCGAGTTTCTGCCAGAGGAGTCGCCTTACGAGCTGGCAAACCC 480


10366R-4.IR_full       481 ACTAACGCCAGAGGAAAATGCAG 503
                           |||||||||||||||||| ||||
10366R-4.in silico     481 ACTAACGCCAGAGGAAAA-GCAG 503

 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  NM_136151.1  CG10366-RA (CG10366), mRNA 
2.07   10  10  NM_136107.2  CG17568-RA (CG17568), mRNA 
0   20  NM_141726.1  CG6254-RA (CG6254), mRNA 
0   NM_132957.1  CG9059-RB, transcript variant B (CG9059), mRNA 
0   NM_167553.1  CG9059-RA, transcript variant A (CG9059), mRNA 
0   11  NM_080191.2  CG4148-RA (wek), mRNA 
0   NM_175991.1  CG10595-RA, transcript variant A (d), mRNA 
0   NM_205940.1  CG10595-RB, transcript variant B (d), mRNA 
0   NM_078805.3  CG5838-RA (Dref), mRNA 
0   11  NM_001014630.1  CG33547-RA (Rim), mRNA 
0   NM_169185.1  CG31513-RA (CG31513), mRNA 
0   NM_169197.2  CG31187-RA (CG31187), mRNA 
0   NM_132483.2  CG1737-RA (CG1737), mRNA 
0   NM_167239.2  CG32677-RA (CG32677), mRNA 
0   NM_141568.2  CG9797-RA (CG9797), mRNA 
0   NM_132346.1  CG3003-RB (CG3003), mRNA 
0   NM_079337.2  CG9206-RA (Gl), mRNA 
0   NM_078922.2  CG4486-RA (Cyp9b2), mRNA 
0   13  NM_137342.2  CG30460-RC, transcript variant C (CG30460), mRNA 
0   13  NM_206150.1  CG30460-RB, transcript variant B (CG30460), mRNA 
0   13  NM_206149.1  CG30460-RA, transcript variant A (CG30460), mRNA 
0   NM_165836.1  CG7776-RB, transcript variant B (E(Pc)), mRNA 
0   NM_078974.2  CG7776-RA, transcript variant A (E(Pc)), mRNA 
0   NM_206148.1  CG30460-RD, transcript variant D (CG30460), mRNA 
0   NM_078681.2  CG32540-RA (CCKLR-17D3), mRNA 
0   NM_057981.3  CG4063-RA (ebi), mRNA 
0   NM_176557.1  CG33106-RB, transcript variant B (mask), mRNA 
0   NM_176556.1  CG33106-RA, transcript variant A (mask), mRNA 
0   NM_165571.1  CG1877-RC, transcript variant C (lin19), mRNA 
0   NM_165570.1  CG1877-RB, transcript variant B (lin19), mRNA 
 Wing Disc
GFP
Puc
Caspase
DAPI
Merge
Figure
JNK signal
N
 
DB
 
DC
 
DM
 
VM
 
VB
 
Caspase-3 signal
N
DB
DC
DM
++ 
VM
++ 
VB
 
Dcompartment   Penetrance
   

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