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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 10361R-1 
 Symbol CG10361  Full Name CG10361 
 CG No CG10361  Old CG No CG10361 
 Synonyms CG10361 
 Accession No (Link to NCBI) NM_140252.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCCAGCTGGCCGGGATTAAGGA 
 Primer Seq. 3' 
aatctagaggtaccTGGCGATCAGCTTGAGGCGAG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CCAGCTGGCC GGGATTAAGG ATGCAGGCAC CTTTAAAGCA GAGCGAATCA TCACATCTTC 
0061 GCAGAGCACT CAGATAACCG TGCAAGGTAG CGATAAAAAG ATACTCAACT TTTGCGCCAA 
0121 CAACTACTTA GGACTGGCGA ACAATCCGGA GATCGTGGAG CACAGCCAGA AGCTGTTGGA 
0181 GCAGTATGGC GCTGGTCTGA GCTCAGTGCG GTTCATTTGC GGCACGCAGG ATATACACAA 
0241 GCAGCTGGAA AAGAAGATAG CCCAGTTCCA TGGGCGCGNA GGATACCATT CTGTACGCCT 
0301 CCTGTTTCGA CGCCAATGCC GGTATCTTTG AGGCCATCCT CACACCGGAG GATGCGGTCT 
0361 TCTCCGATGA ACTAAATCAC GCATCCATCA TTGACGGCAT CCGTCTGTGC AAGGCGAAGA 
0421 AACAGCGCTA TAGGCACCGG GACCTGGGCG ACTTGGAGGA GCAGCTAAAG GCCAGCGATG 
0481 CTCGCCTCAA GCTGATCGCC A 
 in silico PCR Fragment 
0001 CCAGCTGGCC GGGATTAAGG ATGCAGGCAC CTTTAAAGCA GAGCGAATCA TCACATCTTC 
0061 GCAGAGCACT CAGATAACCG TGCAAGGTAG CGATAAAAAG ATACTCAACT TTTGCGCCAA 
0121 CAACTACTTA GGACTGGCGA ACAATCCGGA GATCGTGGAG CACAGCCAGA AGCTGTTGGA 
0181 GCAGTATGGC GCTGGTCTGA GCTCAGTGCG GTTCATTTGC GGCACGCAGG ATATACACAA 
0241 GCAGCTGGAA AAGAAGATAG CCCAGTTCCA TGGGCGCG-A GGATACCATT CTGTACGCCT 
0301 CCTGTTTCGA CGCCAATGCC GGTATCTTTG AGGCCATCCT CACACCGGAG GATGCGGTCT 
0361 TCTCCGATGA ACTAAATCAC GCATCCATCA TTGACGGCAT CCGTCTGTGC AAGGCGAAGA 
0421 AACAGCGCTA TAGGCACCGG GACCTGGGCG ACTTGGAGGA GCAGCTAAAG GCCAGCGATG 
0481 CTCGCCTCAA GCTGATCGCC A 
 Assemble Data 
<PRE>

10361R-1.IR_full       1   CCAGCTGGCCGGGATTAAGGATGCAGGCACCTTTAAAGCAGAGCGAATCATCACATCTTC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10361R-1.in silico     1   CCAGCTGGCCGGGATTAAGGATGCAGGCACCTTTAAAGCAGAGCGAATCATCACATCTTC 60


10361R-1.IR_full       61  GCAGAGCACTCAGATAACCGTGCAAGGTAGCGATAAAAAGATACTCAACTTTTGCGCCAA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10361R-1.in silico     61  GCAGAGCACTCAGATAACCGTGCAAGGTAGCGATAAAAAGATACTCAACTTTTGCGCCAA 120


10361R-1.IR_full       121 CAACTACTTAGGACTGGCGAACAATCCGGAGATCGTGGAGCACAGCCAGAAGCTGTTGGA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10361R-1.in silico     121 CAACTACTTAGGACTGGCGAACAATCCGGAGATCGTGGAGCACAGCCAGAAGCTGTTGGA 180


10361R-1.IR_full       181 GCAGTATGGCGCTGGTCTGAGCTCAGTGCGGTTCATTTGCGGCACGCAGGATATACACAA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10361R-1.in silico     181 GCAGTATGGCGCTGGTCTGAGCTCAGTGCGGTTCATTTGCGGCACGCAGGATATACACAA 240


10361R-1.IR_full       241 GCAGCTGGAAAAGAAGATAGCCCAGTTCCATGGGCGCG<span class="snp"><tt>N</tt></span>AGGATACCATTCTGTACGCCT 300
                           ||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||
10361R-1.in silico     241 GCAGCTGGAAAAGAAGATAGCCCAGTTCCATGGGCGCG<span class="snp"><tt>-</tt></span>AGGATACCATTCTGTACGCCT 300


10361R-1.IR_full       301 CCTGTTTCGACGCCAATGCCGGTATCTTTGAGGCCATCCTCACACCGGAGGATGCGGTCT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10361R-1.in silico     301 CCTGTTTCGACGCCAATGCCGGTATCTTTGAGGCCATCCTCACACCGGAGGATGCGGTCT 360


10361R-1.IR_full       361 TCTCCGATGAACTAAATCACGCATCCATCATTGACGGCATCCGTCTGTGCAAGGCGAAGA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10361R-1.in silico     361 TCTCCGATGAACTAAATCACGCATCCATCATTGACGGCATCCGTCTGTGCAAGGCGAAGA 420


10361R-1.IR_full       421 AACAGCGCTATAGGCACCGGGACCTGGGCGACTTGGAGGAGCAGCTAAAGGCCAGCGATG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10361R-1.in silico     421 AACAGCGCTATAGGCACCGGGACCTGGGCGACTTGGAGGAGCAGCTAAAGGCCAGCGATG 480


10361R-1.IR_full       481 CTCGCCTCAAGCTGATCGCCA 501
                           |||||||||||||||||||||
10361R-1.in silico     481 CTCGCCTCAAGCTGATCGCCA 501

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_140252.1  CG10361-RA (CG10361), mRNA 
0   0  4  4  2  NM_168420.1  CG32067-RA, transcript variant A (simj), mRNA 
0   0  4  4  2  NM_140150.2  CG32067-RC, transcript variant C (simj), mRNA 
0   0  4  4  2  NM_168421.1  CG32067-RB, transcript variant B (simj), mRNA 
0   0  4  2  2  NM_139929.3  CG7387-RA (CG7387), mRNA 
0   0  3  2  2  NM_057378.2  CG4200-RA (sl), mRNA 
0   0  3  2  2  NM_140908.1  CG7570-RA (hale), mRNA 
0   0  1  6  5  NM_135223.2  CG11199-RA, transcript variant A (Liprin-alpha), mRNA 
0   0  1  6  5  NM_164707.1  CG11199-RB, transcript variant B (Liprin-alpha), mRNA 
0   0  1  5  8  NM_141520.2  CG9630-RA (CG9630), mRNA 
0   0  1  5  5  NM_141538.1  CG7352-RA (CG7352), mRNA 
0   0  1  5  3  NM_132217.2  CG10958-RA (CG10958), mRNA 
0   0  1  5  3  NM_166463.1  CG10433-RB, transcript variant B (CG10433), mRNA 
0   0  1  5  3  NM_137746.2  CG10433-RA, transcript variant A (CG10433), mRNA 
0   0  1  3  3  NM_132998.1  CG8326-RA (CG8326), mRNA 
0   0  1  3  2  NM_134738.2  CG4887-RA (CG4887), mRNA 
0   0  1  2  7  NM_132843.1  CG17209-RA (CG17209), mRNA 
0   0  1  2  6  NM_057805.2  CG9660-RA, transcript variant A (toc), mRNA 
0   0  1  2  5  NM_132385.2  CG2967-RA (CG2967), mRNA 
0   0  1  2  4  NM_001043221.1  CG7549-RC, transcript variant C (CG7549), mRNA 
0   0  1  2  4  NM_141518.1  CG7549-RA, transcript variant A (CG7549), mRNA 
0   0  1  2  4  NM_164524.1  CG9660-RD, transcript variant D (toc), mRNA 
0   0  1  2  4  NM_164525.1  CG9660-RC, transcript variant C (toc), mRNA 
0   0  1  2  4  NM_164526.1  CG9660-RB, transcript variant B (toc), mRNA 
0   0  1  2  4  NM_001042864.1  CG9660-RE, transcript variant E (toc), mRNA 
0   0  1  2  3  NM_168802.1  CG32210-RA (CG32210), mRNA 
0   0  1  2  3  NM_165552.2  CG18812-RC, transcript variant C (CG18812), mRNA 
0   0  1  2  3  NM_169212.1  CG7549-RB, transcript variant B (CG7549), mRNA 
0   0  1  2  2  NM_136607.1  CG8078-RA (CG8078), mRNA 
0   0  1  2  2  NM_141569.3  CG8202-RA (CG8202), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway dme00260 : Glycine, serine and threonine metabolism
dme01100 : Metabolic pathways

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