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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 10336R-3 
 Symbol CG10336  Full Name CG10336 
 CG No CG10336  Old CG No CG10336 
 Synonyms CG10336 
 Accession No (Link to NCBI) NM_165261.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees pupal semi-lethal 
 Map Viewer
 Reference
[Please submit your publication]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS ONE (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgATGGCATCCCTGTTCGGAGAT 
 Primer Seq. 3'
aatctagaggtaccTGTTGCTCATCCTGCGCCTCC 
 Predicted Fragment Size
500 
 IR fragment full Seq
0001 ATGGCATCCC TGTTCGGAGA TGATGGTGTG GATGACCTAT TCAATGACAA CATTCCGACT 
0061 GACCCGGATC AGCTGCCCAG CGATGGCGAA GGCGAAAAGC TGTTTGCGGA CGACGAGGAC 
0121 AACGGCGTAG AANCCTGGTA GCCAGGATGC CCAGATAGTG GAGCCTAAGA AGCGAGCTGT 
0181 GAGAAATCCC CGACCGCGAC TCACTGTGGA AACACTTCGA GGTCCTCGTG GCATCCAGAC 
0241 CATCGAAGAT TATTTTAAGG ACATCAAATT CAAGGGCAAG GGTTACGAGA AAACCGATCT 
0301 CGACGAGGTG CTTCGCCGCC TGCAGCACTG GGGCCATCGT ATGTATCCCA CCTACACCTT 
0361 TGACGATGTG CTTAACAATA TCGAACGGCT GGGCAAGAAG AAACCCCTCC AAGTGCACAT 
0421 GGCGCGATAT CGACTCGGTC AGCTGGAGCA GATGCGCGCC CACGAAGCGG AGGCCCTGGA 
0481 GGAGGCGCAG GATGAGCAAC A 
 in silico PCR Fragment
0001 ATGGCATCCC TGTTCGGAGA TGATGGTGTG GATGACCTAT TCAATGACAA CATTCCGACT 
0061 GACCCGGATC AGCTGCCCAG CGATGGCGAA GGCGAAAAGC TGTTTGCGGA CGACGAGGAC 
0121 AACGGCGTAG AA-CCTGGTA GCCAGGATGC CCAGATAGTG GAGCCTAAGA AGCGAGCTGT 
0181 GAGAAATCCC CGACCGCGAC TCACTGTGGA AACACTTCGA GGTCCTCGTG GCATCCAGAC 
0241 CATCGAAGAT TATTTTAAGG ACATCAAATT CAAGGGCAAG GGTTACGAGA AAACCGATCT 
0301 CGACGAGGTG CTTCGCCGCC TGCAGCACTG GGGCCATCGT ATGTATCCCA CCTACACCTT 
0361 TGACGATGTG CTTAACAATA TCGAACGGCT GGGCAAGAAG AAACCCCTCC AAGTGCACAT 
0421 GGCGCGATAT CGACTCGGTC AGCTGGAGCA GATGCGCGCC CACGAAGCGG AGGCCCTGGA 
0481 GGAGGCGCAG GATGAGCAAC A 
 Assemble Data

10336R-3.IR_full       1   ATGGCATCCCTGTTCGGAGATGATGGTGTGGATGACCTATTCAATGACAACATTCCGACT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10336R-3.in silico     1   ATGGCATCCCTGTTCGGAGATGATGGTGTGGATGACCTATTCAATGACAACATTCCGACT 60


10336R-3.IR_full       61  GACCCGGATCAGCTGCCCAGCGATGGCGAAGGCGAAAAGCTGTTTGCGGACGACGAGGAC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10336R-3.in silico     61  GACCCGGATCAGCTGCCCAGCGATGGCGAAGGCGAAAAGCTGTTTGCGGACGACGAGGAC 120


10336R-3.IR_full       121 AACGGCGTAGAANCCTGGTAGCCAGGATGCCCAGATAGTGGAGCCTAAGAAGCGAGCTGT 180
                           |||||||||||| |||||||||||||||||||||||||||||||||||||||||||||||
10336R-3.in silico     121 AACGGCGTAGAA-CCTGGTAGCCAGGATGCCCAGATAGTGGAGCCTAAGAAGCGAGCTGT 180


10336R-3.IR_full       181 GAGAAATCCCCGACCGCGACTCACTGTGGAAACACTTCGAGGTCCTCGTGGCATCCAGAC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10336R-3.in silico     181 GAGAAATCCCCGACCGCGACTCACTGTGGAAACACTTCGAGGTCCTCGTGGCATCCAGAC 240


10336R-3.IR_full       241 CATCGAAGATTATTTTAAGGACATCAAATTCAAGGGCAAGGGTTACGAGAAAACCGATCT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10336R-3.in silico     241 CATCGAAGATTATTTTAAGGACATCAAATTCAAGGGCAAGGGTTACGAGAAAACCGATCT 300


10336R-3.IR_full       301 CGACGAGGTGCTTCGCCGCCTGCAGCACTGGGGCCATCGTATGTATCCCACCTACACCTT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10336R-3.in silico     301 CGACGAGGTGCTTCGCCGCCTGCAGCACTGGGGCCATCGTATGTATCCCACCTACACCTT 360


10336R-3.IR_full       361 TGACGATGTGCTTAACAATATCGAACGGCTGGGCAAGAAGAAACCCCTCCAAGTGCACAT 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10336R-3.in silico     361 TGACGATGTGCTTAACAATATCGAACGGCTGGGCAAGAAGAAACCCCTCCAAGTGCACAT 420


10336R-3.IR_full       421 GGCGCGATATCGACTCGGTCAGCTGGAGCAGATGCGCGCCCACGAAGCGGAGGCCCTGGA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10336R-3.in silico     421 GGCGCGATATCGACTCGGTCAGCTGGAGCAGATGCGCGCCCACGAAGCGGAGGCCCTGGA 480


10336R-3.IR_full       481 GGAGGCGCAGGATGAGCAACA 501
                           |||||||||||||||||||||
10336R-3.in silico     481 GGAGGCGCAGGATGAGCAACA 501

 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  NM_136051.1  CG10336-RB, transcript variant B (CG10336), mRNA 
100   482  NM_165261.1  CG10336-RA, transcript variant A (CG10336), mRNA 
0   NM_140591.2  CG13072-RA (PDCD-5), mRNA 
0   NM_206069.1  CG12919-RB, transcript variant B (egr), mRNA 
0   NM_165735.2  CG12919-RA, transcript variant A (egr), mRNA 
0   NM_132797.2  CG9198-RA, transcript variant A (shtd), mRNA 
0   NM_175991.1  CG10595-RA, transcript variant A (d), mRNA 
0   NM_205939.1  CG10595-RC, transcript variant C (d), mRNA 
0   NM_205940.1  CG10595-RB, transcript variant B (d), mRNA 
0   NM_079193.2  CG1167-RA (Ras64B), mRNA 
0   NM_130677.1  CG14418-RA (CG14418), mRNA 
0   NM_142486.2  CG7708-RA, transcript variant A (CG7708), mRNA 
0   NM_169832.1  CG7708-RB, transcript variant B (CG7708), mRNA 
0   12  NM_001032051.1  CG33715-RD, transcript variant D (Msp-300), mRNA 
0   NM_001032053.1  CG33715-RB, transcript variant B (Msp-300), mRNA 
0   NM_001032052.1  CG33715-RE, transcript variant E (Msp-300), mRNA 
0   NM_137518.2  CG15073-RA (CG15073), mRNA 
0   NM_136223.2  CG9335-RA (CG9335), mRNA 
0   NM_142874.2  CG6747-RA (Ir), mRNA 
0   NM_175995.1  CG32983-RA (CG32983), mRNA 
0   NM_078700.2  CG1849-RA (run), mRNA 
0   NM_136397.1  CG15236-RA, transcript variant A (CG15236), mRNA 
0   NM_165493.1  CG15236-RB, transcript variant B (CG15236), mRNA 
0   NM_176694.1  CG3126-RB, transcript variant B (C3G), mRNA 
0   NM_132122.2  CG3126-RA, transcript variant A (C3G), mRNA 
0   NM_142371.1  CG14330-RA (CG14330), mRNA 
0   NM_138090.1  CG12252-RA (CG12252), mRNA 
0   NM_137246.1  CG10734-RA (CG10734), mRNA 
0   NM_136634.2  CG1975-RA (Rep2), mRNA 
0   NM_132850.2  CG8995-RA (PGRP-LE), mRNA 
 Wing Disc
GFP
Puc
Caspase
DAPI
Merge
Figure
JNK signal
N
 
DB
 
DC
 
DM
 
VM
 
VB
 
Caspase-3 signal
N
++ 
DB
DC
++ 
DM
VM
 
VB
 
Dcompartment   Penetrance
     

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